DOI QR코드

DOI QR Code

Effects of pH on the growth, total nitrogen, total phosphorus and organic compound removal in heterotrophic culture of Chlorella sorokiniana applied wastewater treatment

pH와 탄소원이 Chlorella sorokiniana의 heterotrophic 배양 및 하폐수고도처리능에 미치는 영향

  • Park, Jeong-Eun (Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University) ;
  • Cho, Yong-Beom (Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University) ;
  • Zhang, Shan (Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University) ;
  • Hwang, Sun-Jin (Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University)
  • Received : 2013.11.19
  • Accepted : 2013.12.02
  • Published : 2013.12.15

Abstract

Among many microalgae cultivation types, heterotrophic culture with low cost carbon sources and energy saving culture method is crucial. A result of estimating the effects of pH on wastewater treatment using heterotrophic growing microalgae Chlorella sorokiniana shows that there was no difference in microalgae growth amount and nitrogen, phosphorus removal rate by wide range of pH(5 ~ 9). From pH 5 to 9, total nitrogen, phosphorous and glucose removal rates were 10.5 mg-N/L/d, 2 mg-P/L/d, 800 ~ 1000 mg/L respectively. This study reveals that C. sorokiniana cannot metabolite glycerol heterotrophically, however, glucose and acetate were proper carbon sources for growth and T-N, T-P and TOC removal. This research highlights the potential of heterotrophic microalgal growth with wastewater treatment plant with wide range of pH and carbon sources.

Keywords

References

  1. APHA- AWA-WEF, 2005. Standard Methods for the Examination of Water and Wastewater, 21st ed. American Public Health Association, Washington DC.
  2. Barsanti, L., Gualtieri, P. (2006) Algae:Anatomy, Biochemistry, and Biotechnology, pp.213-214, CRC Press, Boca Raton, FL.
  3. Boyle, N.R., Morgan, J.A. (2009) Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii, BMC syst. Biol. 3, 4. https://doi.org/10.1186/1752-0509-3-4
  4. Brennan, L., Owende, P. (2010) Biofuels from microalgae-A review of technologies for production, processing, and extraction of biofuels and co-products, Renewable and Sustainable Energy Reviews, Vol. 14, pp.557-577. https://doi.org/10.1016/j.rser.2009.10.009
  5. de-Ba shan, L.E., Bashan, Y., Moreno, M., Lebsky, V.K., Bustillos, J.J. (2002) Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growthpromoting bacterium Azospirillum brasilense. Can. J. Microbiol. 48, pp.514-521. https://doi.org/10.1139/w02-051
  6. de-Ba shan, L.E., Hernandez, J.P., Morey, T., Bashan, Y. (2004) Microalgae growth-promoting bacteria as "helpers" for microalgae: a novel approach for removing ammonium and phosphorus from municipal wastewater. Water Research. 38, pp.466-474. https://doi.org/10.1016/j.watres.2003.09.022
  7. Droop, M.R. (1974) Heterotrophy of carbon. In:stewart, W.D.P. (Ed.), Algal Physiology and Biochemistry. Blackwell Scientific, Oxford, UK, pp.530-559.
  8. Harun, R., Singh, M., Forde, G.M., Danquah, M.K. (2010) Bioprocess engineering of microalgae to produce a variety of consumer products.Renewable and Sustainable Energy Reviews, 14, pp.1037-1047. https://doi.org/10.1016/j.rser.2009.11.004
  9. Javanm ardian, M., Palsson, B.O. (1991) Highdensity photoautotrophic algal cultures: design, construction, and operation of a novel photobioreactor system. Biotechnology and Bioengineering, 38, pp.1182-1189 https://doi.org/10.1002/bit.260381010
  10. Kaplan , D., Richmond, A.E., Dubinsky, Z. & Aaronson, A. (1986) Algal Nutrition. In:Richmond, A. (Ed.), Handbook for Microalgal Mass Culture, pp.147-198, CRC Press, Boca Raton, FL.
  11. Komor , E., Tanner, W. (1974) The hexose-proton symport system of Chlorella vulgaris: specificity, stoichiometry and energetics of sugar-induced proton uptake, European Journal of Biochemistry, 44, pp.219-223. https://doi.org/10.1111/j.1432-1033.1974.tb03476.x
  12. Komor , E., Tanner, W. (1976) The determination of the membrane potential of Chlorella vulgaris: evidence for electrogenic sugar transport, European Journal of Biochemistry, 70, pp.197-204. https://doi.org/10.1111/j.1432-1033.1976.tb10970.x
  13. Komor , E., Schobert, C., Cho, B.H. (1985) Sugar specificity and sugar-proton interaction for the hexose-proton-symport system of Chlorella. European Journal of Biochemistry, 146, pp.649-656. https://doi.org/10.1111/j.1432-1033.1985.tb08700.x
  14. Kwon, S. H., Lee, E. M., Cho, D. C. (2012) Optimal culturing and enhancement of lipid accumulation in a microalga Botryococcus braunii, Journal of Korean Environmental Sciences, 21(7), pp.779-785. https://doi.org/10.5322/JES.2012.21.7.779
  15. Mata, T. M., Martins, A. A., Caetano, N. S. (2010) Microalgae for biodiesel production and other applications: A review, Renewable and Sustainable Energy Reviews, 14(1), pp.217-232. https://doi.org/10.1016/j.rser.2009.07.020
  16. Neilson , A.H., Lewin, R.A. (1974) The uptake and utilization of organic carbon by algae: an essay in comparative biochemistry. Phycologia, 13, pp.227-264. https://doi.org/10.2216/i0031-8884-13-3-227.1
  17. Perez- Garcia, O., Escalante, F. M. E., de- Bashan, L. E., Bashan, Y. (2011) Heterotrophic cultures of microalgae: Metabolism and potential products. Water Research. 45, pp.11-36. https://doi.org/10.1016/j.watres.2010.08.037
  18. Prescott, L., Harley, J. P., Klein, D, A. (2003) Microbiology, 5th ed. pp.114-116, McGraw- Hill, New York
  19. Pulz, O . (2001) Photobioreactors: production systems for phototrophic microorganisms. Applied Microbiology and Biotechnology. 57, pp.287-293. https://doi.org/10.1007/s002530100702
  20. Radmer, R.J., Parker, B.C. (1994) Commercial applications of algae: opportunities and constraints. Journal of Applied Phycology, 6, pp.93-98 https://doi.org/10.1007/BF02186062
  21. Tuchm an, N. C. (1996) "The role of heterotrophy in algae. In : Stevenson, R. J., M. L. Bothwell, and R. L. Lowe(eds.)", Algal Ecology : Freshwater Benthic Ecosystems, pp. 299-319, Academic Press, New York.
  22. Wang, H., Xiong, H., Hui, Z., Zeng, X. (2012) Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresource Technology, 104, pp.215-220. https://doi.org/10.1016/j.biortech.2011.11.020
  23. Watanabe, M. (2005) Freshwater culture media. Alagal Culturing Techniques, Elsevier, Amsterdam

Cited by

  1. Decolorization and degradation analysis of Disperse Red 3B by a consortium of the fungus Aspergillus sp. XJ-2 and the microalgae Chlorella sorokiniana XJK vol.9, pp.25, 2013, https://doi.org/10.1039/c9ra01169b