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http://dx.doi.org/10.11001/jksww.2013.27.6.703

Effects of pH on the growth, total nitrogen, total phosphorus and organic compound removal in heterotrophic culture of Chlorella sorokiniana applied wastewater treatment  

Park, Jeong-Eun (Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University)
Cho, Yong-Beom (Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University)
Zhang, Shan (Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University)
Hwang, Sun-Jin (Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University)
Publication Information
Journal of Korean Society of Water and Wastewater / v.27, no.6, 2013 , pp. 703-709 More about this Journal
Abstract
Among many microalgae cultivation types, heterotrophic culture with low cost carbon sources and energy saving culture method is crucial. A result of estimating the effects of pH on wastewater treatment using heterotrophic growing microalgae Chlorella sorokiniana shows that there was no difference in microalgae growth amount and nitrogen, phosphorus removal rate by wide range of pH(5 ~ 9). From pH 5 to 9, total nitrogen, phosphorous and glucose removal rates were 10.5 mg-N/L/d, 2 mg-P/L/d, 800 ~ 1000 mg/L respectively. This study reveals that C. sorokiniana cannot metabolite glycerol heterotrophically, however, glucose and acetate were proper carbon sources for growth and T-N, T-P and TOC removal. This research highlights the potential of heterotrophic microalgal growth with wastewater treatment plant with wide range of pH and carbon sources.
Keywords
Microalgae; Chlorella sorokiniana; Wastewater treatment; Heterotrophic culture;
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1 APHA- AWA-WEF, 2005. Standard Methods for the Examination of Water and Wastewater, 21st ed. American Public Health Association, Washington DC.
2 Barsanti, L., Gualtieri, P. (2006) Algae:Anatomy, Biochemistry, and Biotechnology, pp.213-214, CRC Press, Boca Raton, FL.
3 Boyle, N.R., Morgan, J.A. (2009) Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii, BMC syst. Biol. 3, 4.   DOI   ScienceOn
4 Brennan, L., Owende, P. (2010) Biofuels from microalgae-A review of technologies for production, processing, and extraction of biofuels and co-products, Renewable and Sustainable Energy Reviews, Vol. 14, pp.557-577.   DOI   ScienceOn
5 de-Ba shan, L.E., Hernandez, J.P., Morey, T., Bashan, Y. (2004) Microalgae growth-promoting bacteria as "helpers" for microalgae: a novel approach for removing ammonium and phosphorus from municipal wastewater. Water Research. 38, pp.466-474.   DOI   ScienceOn
6 Droop, M.R. (1974) Heterotrophy of carbon. In:stewart, W.D.P. (Ed.), Algal Physiology and Biochemistry. Blackwell Scientific, Oxford, UK, pp.530-559.
7 Harun, R., Singh, M., Forde, G.M., Danquah, M.K. (2010) Bioprocess engineering of microalgae to produce a variety of consumer products.Renewable and Sustainable Energy Reviews, 14, pp.1037-1047.   DOI   ScienceOn
8 Javanm ardian, M., Palsson, B.O. (1991) Highdensity photoautotrophic algal cultures: design, construction, and operation of a novel photobioreactor system. Biotechnology and Bioengineering, 38, pp.1182-1189   DOI   ScienceOn
9 Kaplan , D., Richmond, A.E., Dubinsky, Z. & Aaronson, A. (1986) Algal Nutrition. In:Richmond, A. (Ed.), Handbook for Microalgal Mass Culture, pp.147-198, CRC Press, Boca Raton, FL.
10 Komor , E., Tanner, W. (1976) The determination of the membrane potential of Chlorella vulgaris: evidence for electrogenic sugar transport, European Journal of Biochemistry, 70, pp.197-204.   DOI   ScienceOn
11 Perez- Garcia, O., Escalante, F. M. E., de- Bashan, L. E., Bashan, Y. (2011) Heterotrophic cultures of microalgae: Metabolism and potential products. Water Research. 45, pp.11-36.   DOI   ScienceOn
12 Komor , E., Schobert, C., Cho, B.H. (1985) Sugar specificity and sugar-proton interaction for the hexose-proton-symport system of Chlorella. European Journal of Biochemistry, 146, pp.649-656.   DOI   ScienceOn
13 Kwon, S. H., Lee, E. M., Cho, D. C. (2012) Optimal culturing and enhancement of lipid accumulation in a microalga Botryococcus braunii, Journal of Korean Environmental Sciences, 21(7), pp.779-785.   DOI   ScienceOn
14 Neilson , A.H., Lewin, R.A. (1974) The uptake and utilization of organic carbon by algae: an essay in comparative biochemistry. Phycologia, 13, pp.227-264.   DOI
15 Prescott, L., Harley, J. P., Klein, D, A. (2003) Microbiology, 5th ed. pp.114-116, McGraw- Hill, New York
16 Pulz, O . (2001) Photobioreactors: production systems for phototrophic microorganisms. Applied Microbiology and Biotechnology. 57, pp.287-293.   DOI   ScienceOn
17 Radmer, R.J., Parker, B.C. (1994) Commercial applications of algae: opportunities and constraints. Journal of Applied Phycology, 6, pp.93-98   DOI   ScienceOn
18 Tuchm an, N. C. (1996) "The role of heterotrophy in algae. In : Stevenson, R. J., M. L. Bothwell, and R. L. Lowe(eds.)", Algal Ecology : Freshwater Benthic Ecosystems, pp. 299-319, Academic Press, New York.
19 Wang, H., Xiong, H., Hui, Z., Zeng, X. (2012) Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresource Technology, 104, pp.215-220.   DOI   ScienceOn
20 de-Ba shan, L.E., Bashan, Y., Moreno, M., Lebsky, V.K., Bustillos, J.J. (2002) Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growthpromoting bacterium Azospirillum brasilense. Can. J. Microbiol. 48, pp.514-521.   DOI   ScienceOn
21 Komor , E., Tanner, W. (1974) The hexose-proton symport system of Chlorella vulgaris: specificity, stoichiometry and energetics of sugar-induced proton uptake, European Journal of Biochemistry, 44, pp.219-223.   DOI   ScienceOn
22 Mata, T. M., Martins, A. A., Caetano, N. S. (2010) Microalgae for biodiesel production and other applications: A review, Renewable and Sustainable Energy Reviews, 14(1), pp.217-232.   DOI   ScienceOn
23 Watanabe, M. (2005) Freshwater culture media. Alagal Culturing Techniques, Elsevier, Amsterdam