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Method Development for Determination of Chlorogenic Acid and Arbutin Contents in Fruits by UHPLC-MS/MS (UHPLC-MS/MS를 이용한 과일류 중 클로로젠산 및 알부틴 동시분석법 개발)

  • Choi, Young-Ju;Jeon, Jong-Sup;Kim, Woon-Ho;Jung, You-Jung;Ryu, Ji-Eun;Choi, Jong-Chul;Chae, Kyung-Suk;Lee, Jin-Hee;Do, Young-Sook;Park, Young-Bae;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.34 no.5
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    • pp.413-420
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    • 2019
  • In this study, a sample preparation method and a simultaneous determination method by ultra-high performance liquid chromatography coupled with tandem mass spectrometry for 9 isomers of chlorogenic acid and arbutin in fruits were developed. The samples were extracted using 90% methanol (pH 3.0), with the solutions being shaken and then sonicated for 10 min each. After centrifugation at 4,000 rpm for 10 min, the extraction was concentrated under a vacuum at $40^{\circ}C$ using a vacuum evaporator. The residue was dissolved in 5 mL of 5% methanol and filtered through a $0.45{\mu}m$ membrane before UHPLC-MS/MS analysis. The separations were performed on a C18 column with gradient elution of water (containing 0.1% formic acid) and methanol (containing 0.1% formic acid). The specificity, linearity, limit of detection, limit of quantification, accuracy, and precision of the proposed methods were also evaluated.

Effects of Chitosan, Grain Amino Acid and Wood Vinegar Foliar Spray on the Quality and Storability of Grapes(Campbell Early) (키토산, 곡물아미노산, 목초액의 엽면살포가 포도(Campbell Early)의 품질 및 저장성에 미치는 영향)

  • Ju, In-Ok;Jung, Gi-Tai;Cheong, Seong-Soo;Moon, Young-Hun;Ryu, Jeong;Choi, Joung-Sik
    • Food Science and Preservation
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    • v.14 no.2
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    • pp.119-123
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    • 2007
  • Sprays containing chitosan, grain amino acids, or wood vinegar, were applied to vine leaves of the Campbell Early grape variety, and effects on the quality and storability of grapes were investigated. Weights of grape clusters and individual bemies did not differ significantly from the values seen when traditional agnicultural chemical treatment was used. The percentage of clusters over 300g in weight was, however, higher after spraying with chitosan, grain amino acids, or wood vinegar, than after agricultural chemical treatment, Grape moisture contents, levels of soluble solids, and reducing sugar concentrations, did not differ when the traditional treatment and the newer sparys were compared. Among minerals, the levels of potassium, iron and zinc measured in fresh grapes were increased by the clitosan, grain amino acids, and wood vinegar spray. After 8 weeks of MA storage, reducing sugar levels decreased, and titratable acidities increased, compared to levels measured at the beginning of storage. This was true regardless of the method of vine treatment the hardness of berries decreased slightly over 4-6 weeks of storage, and increased thereafter. The weight losses of grapes were relatively low(0.28-0.35%) on storage after any vine treatment tested. Grapes from vines sprayed with chitosan or grain amino acids showed a lower decay rate than did fruit from vines that had received a traditional agricultural chemical treatment. Sensory evaluation results indicated that the marketability of grapes from vines treated with traditional agricultural chemicals was better than that of grapes from vines sprayed with chitosan, grain amino acids, or wood vinegar.

Effect of Packaging Systems with High CO2 Treatment on the Quality Changes of Fig (Ficus carica L) during Storage (저장 중 무화과(Ficus carica L) 선도유지를 위한 고농도 이산화탄소 처리된 포장 시스템 적용 연구)

  • Kim, Jung-Soo;Chung, Dae-Sung;Lee, Youn Suk
    • Food Science and Preservation
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    • v.19 no.6
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    • pp.799-806
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    • 2012
  • This experiment was conducted to establish the optimum conditions for high $CO_2$ gas treatment in combination with a proper gas-permeable packaging film to maintain the quality of fig fruit (Ficus carica L). Among the fig fruits with different high $CO_2$ treatments, the quality change was most effectively controlled during storage in the 70%-$CO_2$-treated fig fruit. Harvested fig fruit was packaged using microperforated oriented polypropylene (MP) film to maintain the optimum gas concentrations in the headspace of packaging for the modified-atmosphere system. MP film had an oxygen transmission rate of about $10,295cm^3/m^2$/day/atm at $25^{\circ}C$. The weight loss, firmness, soluble-solid content (SSC), acidity (pH), skin color (Hunter L, a, b), and decay ratio of the fig fruits were monitored during storage at 5 and $25^{\circ}C$. The results of this study showed that the OPP film, OPP film + 70% $CO_2$, and MP film+70% $CO_2$ were highly effective in reducing the loss rate, firmness and decay occurrence rate of fig fruits that were packaged with them during storage. In the case of using treatments with packages of OPP film and OPP film+70% $CO_2$, however, adverse effects like package bursting or physiological injury of the fig may occur due to the gas pressure or long exposure to $CO_2$. Therefore, the results indicated that MP film containing 70% $CO_2$ can be used as an effective treatment to extend the freshness of fig fruits for storage at a proper low temperature.

Compaction Characteristics of Multi-cropping Paddy Soils in South-eastern Part of Korea (우리나라 동남부 다모작 논토양의 경반화 특성)

  • Yun, Eul-Soo;Jung, Ki-Yeul;Park, Ki-Do;Sonn, Yeon-Kyu;Park, Chang-Yeong;Hwang, Jae-Bog;Nam, Min-Hee
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.688-695
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    • 2011
  • This study was carried out for some survey about soil compaction in the multi-cropping system of paddy field. Investigated sites were 90 farmer's fields in south-eastern part of Korea. The tillage practices season was different according to cropping system of paddy; in spring for mono rice cultivation and in autumn for the multi-cropping field. The average tillage depth in investigated sites was about 25 cm, however, it is different between the farmer's tillage practices and soil characteristics. It is high correlation to tillage deep and minimum resistance of penetration. The reaching soil deep to maximum resistance of penetration was about 27 cm, and average penetration resistance of the deep is 1.8~2.0 MPa for moderately fine-textured soils and more than 3.0 MPa for moderately coarse-textured soils. The difference of penetration resistance between cultivating and compacted layer was in order to sandy loam > clayey loam > clayey, and the difference was lesser in poorly drained soils than somewhat poorly ones. In the rice mono cropping field, the maximum resistance in no-tillage for 15 years was 1.18~1.25 Mpa at 20~25 cm in soil deep, however, the resistance of field with every year tillage practices was 2.03~2.21 Mpa. In the extremely compacted sandy loam textured soils, the penetration resistance at 30 cm in soil depth was drastically reduced by the subsoil from 5.2 Mpa to 3.2 Mpa, and the watermelon root in plastic film house was deep elongated.

Effect of Swine Liquid Manure on Soil Chemical Properties and Growth of Rice (Oryza sativa L.) (양돈분뇨 발효액비 시용이 토양 화학성과 벼 (Oryza sativa L.) 생육에 미치는 영향)

  • Lee, Kyu-Hoi;Yoo, Jae-Hong;Park, Eun-Ju;Jung, Yeong-In;Tipayno, S.C.;Shagol, C.C.;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.945-953
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    • 2010
  • This study was conducted to evaluate the effect of swine liquid manure (SLM) on rice grown in Yeonggwang-gun in 2008. The treatments consisted of SLM and chemical fertilizer (CF) based on the recommended amount of nitrogen (11 kg N $10a^{-1}$). The Total N content of the SLM used was 2,881 mg $L^{-1}$. Plant height at the early stage of growth and tiller number were not significantly different between plots applied with swine liquid manure and those with chemical fertilizer in all areas. Plant height at the later stage of growth, lodging and yield were not significantly different between plots applied with swine liquid manure and those with chemical fertilizer in three areas (Baeksu, Gunnam, Beopseong). Plant height at the later stage of growth, as well as lodging were higher in SLM plots than in chemically fertilized plots in Yeonggwang and Yeomsan. However, grain yield was lower in SLM plots than in chemically fertilized plots in these areas. Soil organic matter content and exchangeable cations increased in the swine liquid manure applied plots. Moreover, heavy metal content did not increase in the plots treated with swine liquid manure. Further research to determine the suitable rate of swine liquid manure is needed to reduce lodging damage and to increase the yield and quality of rice.

Chemical components and hepato-protective effect of Lentinula edodes fermented by lactic acid bacteria (표고 유산균 발효물의 성분 및 간기능 보호 효과)

  • Im, Seung-Bin;Kim, Kyung-Je;Jin, Seong-Woo;Koh, Young-Woo;Ha, Neul-I;Jeong, Hee-Gyeong;Lee, Jae-Keun;Yun, Kyeong-Won;Seo, Kyoung-Sun
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.191-199
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    • 2021
  • This study was conducted to improve the useful components and biological activity of Lentinula edodes fermented by lactic acid bacteria (LAB). Three LAB strains (Lactobacillus brevis KCCM 11904, L. plantarum KCCM 354469, and L. fermentum KCCM 12116) were inoculated and used for L. edodes hot water extract (10%, 20%, 30%) fermentation. LAB fermentation of L. edodes hot water extracts decreased pH and thus were more acidic than non-fermented L. edodes hot water extract. β-glucan and ergothioneine contents were increased by L. edodes in a concentration-dependent manner. The ergothioneine and β-glucan contents were highest in fermented with 30% L. edodes hot water extract fermented by L. plantarum and L. brevis (40.48 mg/100 g and 13.94%, respectively). The hepato-protective effect of fermented L. edodes hot water extracts by the three LAB were tested using Sprague-Dawley rat primary hepatocytes. In primary hepatocytes obtained following liver injury induced by acetaminophen, fermented L. edodes hot water extracts by the three LAB showed protective effects, as evident by reduction of the aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase liver markers. The collective results indicate that the fermented L. edodes hot water extracts obtained using LAB are potentially valuable in preventing or treating liver disease.

Physiochemical properties of danyangju and iyangju prepared using uncooked germinated brown rice (무증자 발아현미를 이용한 단양주와 이양주의 이화학적 특성)

  • Ryu, Ji Soo;Shin, Jee Eun;Cho, Min A;Shin, Jang ho;Choi, Hyeon-Son
    • Korean Journal of Food Science and Technology
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    • v.53 no.5
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    • pp.648-656
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    • 2021
  • This study analyzed the physicochemical properties of Danyangju and Iyangju fermented using uncooked germinated brown rice. Total bacteria and yeast from Danyangju and Iyangju showed the maximal value on day 1 of fermentation, but their numbers decreased by the seventh day. On the seventh day, the total bacteria were higher in Iyangju than in Danyangju, but yeast was the lowest in Iyangju. Both Danyangju and Iyangju showed decreased whiteness in color, whereas redness and yellowness increased with fermentation. Sugar contents, reducing sugar, acidity, alcohol, and polyphenol contents increased during fermentation in both Danyangju and Iyangju; this increase was higher in Iyangju than in Danyangju. Iyangju and Danyangju showed gradual increases in α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity until day 5. The γ-aminobutyric acid (GABA) content increased with fermentation in Iyangju and Danyangju, and Iyangju showed over 10-fold increase after fermentation.

Production of yuzu granules using enzyme treated yuzu pulp powder and evaluation of its physiochemical and functional characterization (유자박 식이섬유를 이용한 유자과립 제조 및 이화학적 특성조사)

  • Seong, Hyeon Jun;Lee, Bo-Bae;Kim, Duck-Hyun;Lee, Seung-Hyun;Ha, Ji-Young;Nam, Seung-Hee
    • Korean Journal of Food Science and Technology
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    • v.53 no.4
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    • pp.382-390
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    • 2021
  • In this study, solubilized yuzu pulp powder (EYP) was produced using enzyme treated yuzu pulp powder (YP) and used to manufacture yuzu granules (0-20% EYP content). The physicochemical, product stability, and functional properties of Yuzu granules were compared among five enzyme treatments. Among the five treatments, CL had the highest YP solubilization yield (48.68%). Microstructural observation of EYP using FE-SEM revealed that its surface became irregular and porous after enzymatic treatment. Compared to YP, EYP had 2 times lower insoluble dietary fibers and 3 times lower hemicellulose and cellulose content. Among the yuzu granules, IV (yuzu granules with 15% EYP) had an excellent water and oil holding capacity and flowability. IV granule had the highest narirutin and hesperidin content of 3.4 mg and 2.2 mg/g DW, respectively and the highest antioxidant (68.4%) and tyrosinase inhibitory activities (82.5%). Therefore, EYP or granule with EYP can be used as a functional component in food industry or pharmaceutical field.

Economic Analysis, Growth and Pests of Wheat (Triticum aestivum L.) in Gelatin·Chitin Microorganisms-treated Organic Culture (젤라틴·키틴분해미생물을 이용한 밀 유기재배와 관행재배의 생육, 병해충 발생조사 및 경제성 분석)

  • Ahn, Philip;Lee, Jiho;Cha, Kwang-Hong;Seo, Dong-Jun;An, Kyu-Nam;Yoon, Chang-Yong;Kim, Kil-Yong;Jung, Woo-Jin
    • Korean Journal of Organic Agriculture
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    • v.29 no.2
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    • pp.223-240
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    • 2021
  • This study was carried out to investigate the economic value of organic wheat production using gelatin·chitin microorganisms in Gwangsan-gu, Gwangju city. The soil condition of experiment field was clay loam Jisan series. The organically cultivated fields were sprayed gelatin and chitin degrading bacteria. The test was performed at conventionally cultivated field and organically cultivated field. Emergence of weed on organically cultivated field was significantly higher than conventionally cultivated field which sprayed herbicide before seeding. Weed emergence have a critical impact on grain yield. Occurrence of diseases and insect pests were higher than conventionally cultivated fields. In 2019, the amount of lodging in conventionally cultivated field were higher than conventionally cultivated field. In 2020, lodging and wet injury were occur in both field. Comparing yield element between organically and conventionally cultivated experimental area, grain yield in organically cultivated field was shown slightly higher amount than conventionally cultivated field. However in the actual yield of 2019, organically cultivated field shows 20% deceased yield because of overgrown weed. In 2020, weed emergence and yellow mosaic virus by wet injury cause 30% decease in the grain yield in organically cultivated field. Content of protein, carbohydrates, ash, water and fat in the grain were not different significance. In 2019, net incomes of conventionally cultivated wheat was 461,031 won/0.1 ha while organically cultivated wheat was 443,437 won/0.1 ha. In the rate of income, conventionally cultivated field was 83.0% as against organically cultivated field (73.3%). In 2020, net incomes of organically cultivated wheat was 437,812 won/0.1 ha while conventionally cultivated wheat was 418,281 won/0.1 ha. In the rate of income, conventionally cultivated field was 81.6% as against organically cultivated field (73.0%).

A Study on the Botany of New Natural Habitats of Abeliophyllum distichum Nakai in the Byeonsanbando National Park (변산반도국립공원 내 새로운 미선나무 자생지의 식물학적 연구)

  • Oh, Hyun Kyung;Soh, Min Seok;Rho, Jae Hyun
    • Korean Journal of Heritage: History & Science
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    • v.44 no.2
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    • pp.4-25
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    • 2011
  • This study was performed in 2010 to examine the flora and vegetation structure and chemical characteristics of soil in the growing community of Abeliophyllum distichum, located in the Byeonsanbando National Park. This Abeliophyllum distichum community has more individual numbers in Cheongrim-ni and Jungkye-ri, Byeonsan-myeon, and Buan-gun area, which is designated as a Natural Monument (No. 370), and also where the habitat conditions for Abeliophyllum distichum is more favorable. The authors recorded 100 taxa with 45 families, 82 genus, 93 species, 4 varieties, and 3 forms. Among them, species such as Abeliophyllum distichum (critically endangered), Asarum maculatum (near threatened) and Chionanthus retusa (near threatened), which are categorized as rare plants, were recorded. According to the list of Korean endemic plants, 4 taxa, particularly Philadelphus schrenckii, Abeliophyllum distichum, Weigela subsessilis, and Lonicera subsessili, were recorded. The community of Abeliophyllum distichum is located in the northwest slope of Baekcheon watershed and the community is comprised of healthy soil. The community structure was classified into three: the Castanea crenata community, Zelkova serrata community, and Quercus serrata community. The Castanea crenata community is composed of the Cornus walteri, Platycarya strobilacea, Zelkova serrata, Rhamnella frangulioides, arranged in terms of importance percentage. The Zelkova serrata community is composed of Celtis sinensis, Quercus aliena, Styrax japonica, and Acer pseudo-sieboldianum, also according to importance percentage. As for the Quercus serrata community, it is composed of Quercus variabilis, Castanea crenata, and Prunus sargentii, also arranged in terms of importance percentage. The importance percentage of Abeliophyllum distichum is 6.6% in the Castanea crenata community, 5.6% in the Zelkova serrata community and 5.1% in the Quercus serrata community. Moreover, in order of chemical characteristics of soil pH, electrical conductivity, available phosphoric, organic matter, and exchangeable cation (K, Ca, Mg) are analyzed. The No. 3 site was relatively higher than other districts of the same chemical characteristics of soil.