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Response Surface Optimization of Fermentation Parameters for Citric Acid Production in Solid Substrate Fermentation (고체발효에서 반응표면분석법을 이용한 구연산 생산 최적화)

  • Kim, Jin-Woo
    • Korean Chemical Engineering Research
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    • v.50 no.5
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    • pp.879-884
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    • 2012
  • In this present study, Aspergillus niger NRRL 567 was cultivated on an inert support material and the effects of various fermentation parameters including temperature, nutrient solution pH, inoculation level, and moisture content were observed and optimized by one-factor-at-a-time (OFAT) and response surface methodology (RSM), sequentially. It was found that the incubation temperature of $30^{\circ}C$ with 75% moisture content, nutrient solution pH of 7.1 and inoculation level of $4.0{\times}10^6$ spores/ml were the most favorable. Again, fermentation parameters were optimized using RSM. The determined optimum condition is $26.5^{\circ}C$, pH 9.9, 75.1%, and $6.0{\times}10^6$ spores/ml. Under this optimized condition, A. niger NRRL 567 produced 118.8 g citric acid/kg dry peat moss at 72 hr. Maximum citric acid production of optimized condition by RSM represented a 1.6-fold increase compared to that obtained from control experiment.

Quality Characteristics of Curcuma Longa L. Cookies Prepared with Various Levels of Rice Flour (쌀가루 첨가량을 달리한 울금 쿠키의 품질특성)

  • Choi, Sang-Ho
    • Culinary science and hospitality research
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    • v.18 no.3
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    • pp.215-226
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    • 2012
  • Physicochemical and sensory characteristics of Curcuma Longa L cookies prepared with various levels of rice flour were investigated in this study. There were significant differences in the density of dough, and it had the highest value of 1.32 in the control group. The pH and moisture content of dough increased by addition of rice flour. Spread factor of cookies was significantly decreased by addition of rice flour. DPPH radical scavenging activity of the control group was 58.72%, whereas cookies with rice flour ranged from 59.26~61.41%. The L value of brightness was increased, but a and b value were decreased significantly by addition of rice flour. The hardness of the control group was higher than that of the cookies prepared with different levels of rice flour. Sensory evaluation scores in terms of appearance, flavor, taste, texture and overall preference for cookies showed that 50% and 75% substituted sample groups were higher than those of the other groups. The results of this study suggest that addition of 50~75% rice flour is the best substitution ratio for rice cookies added with Curcuma Longa L.

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Tuna by-product meal as a dietary protein source replacing fishmeal in juvenile Korean rockfish Sebastes schlegeli

  • Kim, Kyoung-Duck;Jang, Ji Won;Kim, Kang-Woong;Lee, Bong-Joo;Hur, Sang Woo;Han, Hyon-Sob
    • Fisheries and Aquatic Sciences
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    • v.21 no.8
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    • pp.29.1-29.8
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    • 2018
  • This study was conducted to evaluate the use of tuna by-product meal (TBM) as a substitute for fishmeal in juvenile Korean rockfish (Sebastes schlegeli) diets. Five isonitrogenous (52% crude protein) and isocaloric (4.9 kcal/g) diets were prepared. Control diet (Con) was formulated to contain brown fishmeal, whereas 25, 50, 75, and 100% of fishmeal was substituted with the same percentage of TBM in the TBM25, TBM50, TBM75, and TBM100 diets, respectively. Three replicate groups of fish (initial weight, $29.5{\pm}0.6g$) were fed one of the five diets for 12 weeks. The mean weight gain of fish fed the Con and TBM25 diets was significantly higher than that of fish fed the TBM100 diet (P < 0.05), but it was not significantly different from the weight gain of fish fed the TBM50 and TBM75 diets. The feed efficiency and protein efficiency ratios of fish fed the Con diet were significantly higher than those of fish fed the TBM100 diet (P < 0.05), but were not significantly different from those fed the TBM25, TBM50, and TBM75 diets. The TBM25, TBM50, TBM75, and TBM100 diets had significantly (P < 0.05) lower incidence cost and higher profit index than the Con diet. These results suggest that the tuna by-product meal used in this study could replace up to 75% of fishmeal protein in the diet without reducing growth and feed utilization in juvenile Korean rockfish weighing 29-53 g. By considering feed cost, the TBM 75 diet is recommendable for Korean rockfish farming.

Efficacy evaluation of novel organic iron complexes in laying hens: effects on laying performance, egg quality, egg iron content, and blood biochemical parameters

  • Jiuai Cao;Jiaming Zhu;Qin Zhou;Luyuan Zhao;Chenhao Zou;Yanshan Guo;Brian Curtin;Fei Ji;Bing Liu;Dongyou Yu
    • Animal Bioscience
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    • v.36 no.3
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    • pp.498-505
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    • 2023
  • Objective: This study was conducted to determine the optimal dose of novel iron amino acid complexes (Fe-Lys-Glu) by measuring laying performance, egg quality, egg iron (Fe) concentrations, and blood biochemical parameters in laying hens. Methods: A total of 1,260 18-week-old healthy Beijing White laying hens were randomly divided into 7 groups with 12 replicates of 15 birds each. After a 2-wk acclimation to the basal diet, hens were fed diets supplemented with 0 (negative control, the analyzed innate iron content was 75.06 mg/kg), 15, 30, 45, 60, and 75 mg Fe/kg as Fe-Lys-Glu or 45 mg Fe/kg from FeSO4 (positive control) for 24 wk. Results: Results showed that compared with the negative and positive control groups, dietary supplementation with 30 to 75 mg Fe/kg from Fe-Lys-Glu significantly (linear and quadratic, p<0.05) increased the laying rate (LR) and average daily egg weight (ADEW); hens administered 45 to 75 mg Fe/kg as Fe-Lys-Glu showed a remarkable (linear, p<0.05) decrease in feed conversion ratio. There were no significant differences among all groups in egg quality. The iron concentrations in egg yolk and serum were elevated by increasing Fe-Lys-Glu levels, and the highest iron content was found in 75 mg Fe/kg group. In addition, hens fed 45 mg Fe/kg from Fe-Lys-Glu had (linear and quadratic, p<0.05) higher yolk Fe contents than that with the same dosage of FeSO4 supplementation. The red blood cell (RBC) count and hemoglobin content (linear and quadratic, p<0.05) increased obviously in the groups fed with 30 to 75 mg Fe/kg as Fe-Lys-Glu in comparison with the control group. Fe-Lys-Glu supplementation also (linear and quadratic, p<0.05) enhanced the activity of copper/zinc-superoxide dismutase (Cu/Zn-SOD) in serum, as a result, the serum malonaldehyde content (linear and quadratic, p<0.05) decreased in hens received 60 to 75 mg Fe/kg as Fe-Lys-Glu. Conclusion: Supplementation Fe-Lys-Glu in laying hens could substitute for FeSO4 and the optimal additive levels of Fe-Lys-Glu are 45 mg Fe/kg in layers diets based on the quadratic regression analysis of LR, ADEW, RBC, and Cu/Zn-SOD.

Response of Growth Characteristics and Yield of Silage Corns at Different Regions (재배지역별 사일리지용 옥수수 일반생육 및 수량 반응)

  • Son, Beom-Young;Kim, Jung-Tae;Lee, Jin-Seok;Baek, Seong-Bum;Kim, Wook-Han;Kim, Jong-Duk;Ko, Ki-Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.3
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    • pp.251-260
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    • 2011
  • This study was carried out to compare growth characteristics and yield of silage corn hybrids at four different regions (Hwaseong, Dangjin, Gochang and Pyeongchang). Two domestic corn hybrids, 'Kwangpyeongok' and 'Gangdaok', and two imported corn hybrids, 'P3394' and '32P75' were used in this study. Plant height and ear height ratio were not significantly different among corn hybrids. 'Kwangpyeongok' was the strongest of all corn hybrids for lodging. The others except 'Kwangpyeongok' were similar to lodging. All corn hybrids were excellent to stay-green. Incidence of southern corn leaf blight (Bipolaris maydis) was low at all corn hybrids. '32P75' was 10.1%, the most of all corn hybrids for incidence of BSDV (black streaked dwarf virus). 'Kwangpyeongok' and 'Gangdaok' were stronger than '32P75' for BSDV. Fresh yield of 'Kwangpyeongok' and 'Gangdaok' were the most of all corn hybrids as 74.85 and 76.68 ton/ha and 'P3394' was the least of all corn hybrids as 64.97 ton/ha. Dry matter yield as 18.83~21.92ton/ha and TDN yield as 12.73~14.52 ton/ha were not significantly different among corn hybrids.

THE EFFECT OF RICE STRAW-POULTRY MANURE SILAGE AND BARLEY ON THE NITROGEN DIGESTION AND MICROBIAL PROTEIN SYNTHESIS IN THE RUMEN OF SHEEP

  • Lee, Nam-Hyung;Yoon, Chil-Surk
    • Asian-Australasian Journal of Animal Sciences
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    • v.2 no.4
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    • pp.615-623
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    • 1989
  • Three sheep fitted with rumen cannulae and abomasal cannulae were given daily 750 g (DM) of three diets consisting of straw-manure silage and barley mixture in the ratios of 75:25, 50:50 and 25:75. As the proportion of barley in the diet increased, there was an increase in the amount of OM apparently digested in the rumen and thole tract (P<.01). But ADF digestion was decreased. For the 25:75 diet the $NH_3-N$ content in the rumen showed the highest value, but the total VFA was the lowest. The rumen volume and dilution rate increased with increasing ratio of silage in diets. There were no significant differences between diets in abomasal NAN flow, and the bacterial-N for the 25:75 diet was 7.3 g N as compared with 9.2-9.6 g N for the other diets (P<.01). Rates of bacterial nitrogen synthesis in the rumen were 30.5, 24.1 and 14.9 g N per Kg OM apparently digested in the rumen for the 75:25, 50:50 and 25:75 diets, respectively.

The Effect of Metformin in Non-Obese Women with Polycystic Ovary Syndrome; Pilot Study (비만하지 않은 다낭성난소증후군 환자에서 메트포민 효용성의 예비 연구)

  • Kim, Hyeong-Ok;Kim, Kye-Hyun
    • Clinical and Experimental Reproductive Medicine
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    • v.35 no.3
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    • pp.223-229
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    • 2008
  • Objective: This pilot study was performed to investigate the effect of metformin on insulin resistance, hormone levels, and lipid profiles in non-obese patients with polycystic ovary syndrome. Methods: This study included 16 non-obese patients with polycystic ovary syndrome diagnosed at our hospital from June 2006 to September 2007. Blood samples were collected before and 6 months after metformin treatment for analysis of fasting serum glucose levels, fasting serum insulin levels, a glycemic response to 75 g oral glucose tolerance test (OGTT), and hormonal blood profile including FSH, LH, estradiol, testosterone, free testosterone, serum lipid profiles. Insulin resistance was estimated by calculating fasting glucose/insulin ratio (FGIR), 2 hr glucose/insulin ratio after 75 g glucose load. And we investigated insulin resistance and pancreatic beta cell function by calculating HOMA beta cell function and HOMA IR. Results: After the treatment of metformin, there was significant increase in 2 hr glucose/insulin ratio after 75 g glucose load (p=0.04) and decrease in HOMA IR (p=0.000). But serum lipid profiles did not change significantly. Also the metformin treatment induced a significant reduction in serum free testosterone and LH levels, and LH/FSH ratio (p=0.001, p=0.000, p=0.034). Conclusion: This pilot study showed that metformin might be effective in improving insulin sensitivity, ameliorating hyperandrogenemia in non-obese patients with polycystic ovary syndrome. Further investigations with larger number of patients and long-term observations are necessary to determine the role of metformin.

Effects of Mustard Seed Extract on Physicochemical and Storage Characteristics of Dry-aged Pork Loin Ham

  • Han-Gyeol Cho;Hack-Youn Kim
    • Food Science of Animal Resources
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    • v.43 no.6
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    • pp.961-974
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    • 2023
  • This study investigated the effects of mustard seed extracts on physicochemical and storage characteristics of dry-aged pork loin ham during the aging period. In experiment 1, antioxidant activity was assessed for mustard seed extracted with varying ethanol concentrations and the results showed high antioxidant activity at 25%, 50%, and 75% ethanol concentrations. In experiment 2, pork loin was treated with mustard seed extracts obtained using different ethanol concentrations: not treated (control), 25% (MS25), 50% (MS50), and 75% (MS75). Physicochemical and storage characteristics of pork loin ham were measured in wk 0, 2, 4, and 6. The pH, aw, CIE b*, thiobarbituric acid reactive substances and volatile basic nitrogen values were lower in treated samples compared to the control (p<0.05). In conclusion, applying mustard seed extracts, particularly MS75, in the dry-aged pork loin ham production process could enhance storage stability and improve color attributes without having negative impacts on product quality.

The Study of Polar Effects of Aluminium Sulfate on the Reduction of Phenolic Resin Spreading Content for the Manufacture of Plywood (황산(黃酸)알루미늄의 극성효과(極性效果)로 인(因)한 합판용(合板用) 페놀 수지(樹脂) 도포량(塗布量)의 감소(減少)에 관(關)한 연구(硏究))

  • Lee, Joung-Sin;Lee, Hwa-Hyoung
    • Journal of the Korean Wood Science and Technology
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    • v.14 no.2
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    • pp.29-35
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    • 1986
  • This study was carried out to examine the effect of aluminium sulfate addition to the alkali-acid catalyst phenolic resin for the manufacture of the kapur (Dryobalanops spp.) plywood on the reduction of phenolic resin spreading. On the manufacture of plywood, Adhesive Contents such as 50g/$m^2$, 75g/$m^2$ and 150g/$m^2$ were treated. The spreading adhesive content of 50g/$m^2$ and 75g/$m^2$ had been controlled to about 150g/$m^2$ added with the water in order to get sufficient spreading and controlled to pH 4.5 with aluminium sulfate [$Al_2(SO_4)_3$]. The results are summarized as follows: 1. Specific gravities of air dried plywood manufactured from each adhesive ranged from 0.77 to 0.86 and their moisture contents met the KS requirements. 2. In dry and wet shear strengths, 150 $P_{Al{\cdot}Ac}$ adhesive showed the highest and 75 $P_{Al{\cdot}Ac{\cdot}Am}$ adhesive indicated higher value than 150 $P_{Al{\cdot}Ac}$ adhesive. 3. In case of glue shear strength after boiling test, 150 $P_{Al{\cdot}Ac}$ adhesive was the best and adding of aluminium sulfate was not effective on reinforcement of boiling water resistance of phenolic resin, but met KS requirements. 4. 75 $P_{Al{\cdot}Ac{\cdot}Am}$ adhesive showed the good shear strength and met KS requirements. Therefore, adding of aluminium sulfate was very efficient for economical plywood manufacture.

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A Clinical Study on Presbyopia in Wearing the Near Spectacle for the First Time (근용 안경을 최초 장용하는 노안에 대한 임상적 연구)

  • Yoo, Jong-Sook;Lim, Hyeon-Seon;Lee, Soo-Cheon;Hwang, Jung-Hee;Kim, Hyo-Jung
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.4
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    • pp.103-107
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    • 2008
  • Purpose: This research measured the near addition powers of presbyopia in wearing the near spectacle for the first time. Methods: We evaluated age, sex, working group, spherical power and cylindrical power and measured the power of refractive error performed the both eyes (310 eyes) of 155 subjects subjectively noncycloplegic and power of addition using the cross cylinder method. Results: There were measured the near addition powers in the range of +0.75D ~ +1.75D. In case of the average addition, they were measured with +1.06D in subjects under 43 years of age, +1.08D in 44 to 46 years, +1.23D in 47 to 49 years and +1.46D in over 50 years of age. The average age in subjects was 47.6 years old and 74 male, 81 female, 71 near sighted working group and 83 far sighted working group. Conclusions: Near addition was correlated with age, working group and cylindrical power (P<0.05, P<0.05, P<0.05).

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