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Development and Evaluation of Validity of Dish Frequency Questionnaire (DFQ) and Short DFQ Using Na Index for Estimation of Habitual Sodium Intake (나트륨 섭취량 추정을 위한 음식섭취빈도조사지와 Na Index를 이용한 간이음식섭취빈도조사지의 개발 및 타당성 검증에 관한 연구)

  • Son, Sook-Mee;Huh, Gwui-Yeop;Lee, Hong-Sup
    • Korean Journal of Community Nutrition
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    • v.10 no.5
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    • pp.677-692
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    • 2005
  • The assessment of sodium intake is complex because of the variety and nature of dietary sodium. This study intended to develop a dish frequency questionnaire (DFQ) for estimating the habitual sodium intake and a short DFQ for screening subjects with high or low sodium intake. For DFQ112, one hundred and twelve dish items were selected based on the information of sodium content of the one serving size and consumption frequency. Frequency of consumption was determined through nine categories ranging from more than 3 times a day to almost never to indicate how often the specified amount of each food item was consumed during the past 6 months. One hundred seventy one adults (male: 78, female: 93) who visited hypertension or health examination clinic participated in the validation study. DFQ55 was developed from DFQ112 by omitting the food items not frequently consumed, selecting the dish items that showed higher sodium content per one portion size and higher consumption frequency. To develop a short DFQs for classifying subjects with low or high sodium intakes, the weighed score according to the sodium content of one protion size was given to each dish item of DFQ25 or DFQ14 and multiplied with the consumption frequency score. A sum index of all the dish items was formed and called sodium index (Na index). For validation study the DFQ112, 2-day diet record and one 24-hour urine collection were analyzed to estimate sodium intakes. The sodium intakes estimated with DFQ112 and 24-h urine analysis showed $65\%$ agreement to be classified into the same quartile and showed significant correlation (r=0.563 p<0.05). However, the actual amount of sodium intake estimated with DFQ112 (male: 6221.9mg, female: 6127.6mg) showed substantial difference with that of 24-h urine analysis (male: 4556.9mg, female: 5107.4mg). The sodium intake estimated with DFQ55 (male: 4848.5mg, female: 4884.3mg) showed small difference from that estimated with 24-h urine analysis, higher proportion to be classfied into the same quartile and higher correlation with the sodium intakes estimated with 24-h urine analysis and systolic blood pressure. It seems DFQ55 can be used as a tool for quantitative estimation of sodium intake. Na index25 or Na index14 showed $39\~50\%$ agreement to be classified into the same quartile, substantial correlations with the sodium intake estimated with DFQ55 and significant correlations with the sodium intake estimated with 24-h urine analysis. When point 119 for Na index25 was used as a criterion of low sodium intake, sensitivity, specificity and positive predictive value was $62.5\%,\;81.8\%\;and\;53.2\%$, respectively. When point 102 for Na index14 was used as a criterion of high sodium intake, sensitivity, specificity and positive predictive value were $73.8\%,\;84.0\%,\;62.0\%$, respectively. It seems the short DFQs using Na index 14 or Na index25 are simple, easy and proper instruments to classify the low or high sodium intake group.

Studies on the Processing of Chestnut (Castanea pubinervis Schneid) Part 1. Trials on the Raw Material Adaptability for Processing and Colored Products Development (밤(Castanea pubinervis schneid) 가공(加工)에 관(關)한 연구(硏究) 제1보 밤의 가공(加工) 적성(適性) 및 유색(有色) 가공품(加工品) 개발(開發)을 중심(中心)으로)

  • Suh, K.S.;Han, P.J.;Lee, S.J.
    • Korean Journal of Food Science and Technology
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    • v.6 no.2
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    • pp.98-108
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    • 1974
  • Fourteen varieties of Korean chestnut were subjected to the test of adaptability for processing and the possibilities of colored products development. The fruit size of Korean chestnut ranged $11{\sim}18g$ and these small fruits could not be expected to be utilized for the processing of Marron glaces which requires larger size as $25{\sim}30g$. As the storge period is extended the discoloration tendency of chestnut flesh was increasingly developed, however, the fresh chestnut has shown layer-separating phenomenon and ragged surface of fruit which delivers disagreeable appearance to the finished product. The principal factors of discoloration occurred during processing were the behavior of tannin and darkening rate shown on flesh differed each other among varieties; the Chukpa and Yuma variety have exhibited the most serious discoloration and the Taab-b variety, the lightest. Taab-b variety in this connection could be expected to be available for Kanroni processing. For the industrialization of chestnut processing the flame-scorched peeling method is advisable. The capacity of this method is proportional to the square of scorching radius and highly flexible in controlling its performance. As for the processing of colored product, the sugar dehydration and coating and the sugar penetration method demand further study in basical views; however, the canned product of chestnut-redbean has shown the possibility of being utilized as a substitute for or paralleled use with the sugar-syruped canned product which so far has been considered as the only item of export to Japan.

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The Comparison of Glomerular Filteration Rate by Kidney Depth in Dynamic kidney Scan (동적신장검사에서 신장깊이에 따른 사구체여과율 비교)

  • Hwang, Ju-Won;Lim, Young-Hyen;Yun, Jong-Jun;Lee, Hwa-Jin;Lee, Mu-Seok;Jung, Ji-Uk;Park, Se-Yun
    • The Korean Journal of Nuclear Medicine Technology
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    • v.18 no.2
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    • pp.73-77
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    • 2014
  • Purpose Find out about the significance of the GFR values calculated by the kidney depth is measured by comparing the values obtained for kidney depth was measured GFR in the CT image kidney depth and is calculated by Tonnesen law in $^{99m}Tc$-DTPA dynamic kidney scan with each applies. Materials and Methods Among patients with normal value (75~120 mL/min) computed GFR conducted of dynamic renal scan to visit from February 2013 to February 2014 and donor GFR values in patients with normal value. The mean age was 46.9 years with 14 men 13 females. We used abdomen CT image which checked before conducting dynamic Kidney scan for measuring the depth of kidney. We only used CT image that contains renal hilum and measured outermost front of the kidney from the skin surface (a) and the final surface (b) caculated the average depth of [(a + b) / 2] respectively. Using the same ROI in order to limit the change in GFR values by the other additional element was set before and after the depth value was excluded from the GFR falls kidney disease. Results Using Tonnesen law the average value was caculated 5.94 cm from the right kidney 5.90 cm from the left kidney. It was 6.83 cm, 8.71 cm in the left kidney and the right kidney average value of the depth measured on the basis of the CT image. The respective increase in left kidney 0.93 cm and right kidney 2.77 cm calculated on the basis of CT image actually measured values. GFR was calculated as the average depth of the subject calculated by the method Tonnesen $83.3{\pm}9.79mL/min$. $98.6{\pm}14.07mL/min$ GFR was applied to calculate the average depth of the subjects using the CT image, is the difference appears 15.26 mL/min was increased after seting up depth value, P value was less than 0.01 which is significant. Conclusion The difference between GFR before-after setting up depth value cause that the different of depth value. Is a measured depth of the extension value of the calculated estimates Whereas Tonnesen kidney depth method is to use in calculating the value of GFR in a typical dynamic elongation test depth derived using the CT image depth. Is thought to be able to calculate more accurately the GFR value by the distance to the center of kidney more accurately measured in the skin thereby.

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Epidemiological Studios on Clonorchis sinensis Infection along the Nam-river in Gyeongnam Province, Korea (남강유역 간흡충감염의 역학적 조사연구)

  • 배경훈;안영겸
    • Parasites, Hosts and Diseases
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    • v.21 no.2
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    • pp.116-186
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    • 1983
  • An epidemiological study on Clonorchis sinensis infection along the Nam-river (total length ; 186km) flowing in Gyeongsang-nam-do, southern part of Korea, was carried out. Formalin-ether concentration technique and Stoll's egg counting method were employed to figure out the prevalence of C. sinensis infection. For the detection of cercariae from Parafossarulus sp., the snail host of C. sinensis, each snail was placed in aerated tap water, and examined for expelled cercariae. For observing the metacercariae the fresh water fish favorably eaten in raw conditions wore prepared by means of pressing the muscles between two slide glasses and/or by digesting them with artificial gastric juice. The fresh water fish were fed to the rabbits to get the worms and to identify the morphology of adult C. sinensis. The results are summarized as follows: 1. Overall Prevalence of C. sinensis infection was 38.75 from 5,291 examinees; 44.15 (1,408 out of 3,196) in male and 30.4% (637 out of 2,095) in female. 2. The prevalence rates were 42.0% at the upper stream, 41.2% around the vicinity of Jinyanglake, 34.2% at Jinju city, 34.2% at middle stream and 40.3% at down stream regions, respectively. 3. By age, the highest positive rate (53.4∼54.3%) was observed in 30 to 59 years of age. In this age group, the rate in males was 59.7∼62.2%, and in females 42.2∼44.4%, In the age group of less than 19 years it was 7.5∼20.9%. 4. By social strata, the positive rate was 16.5% in the primary school children, 22.6% in school students, 46.2% in teachers and local officers and 49.6% in the general inhabitants. 5. The quantitative examinations with the stool collected from clonorchiasis cases revealed that the light infection (less than 4,000/EPG) was 53.6%, moderate infection (4,001∼10,000 /EPG) 30.3% and heavy infection (more than 10,001/EPG) 16.1%, respectively. More than half of total cases examined were light infection, and 73.2% of female examinees were lightly infected with this cuke. 6. The average value of EPG was 4,963 (male, 6,057; female, 2,557 and the highest value was obtained from the age group of 30 to 59 years (5,240∼6,454). 7. The prevalence of Metagonimus yokogawai infection in humans was 5.5%, and 89.8% of metagonimiasis cases wore double-infected arith C. sinensis. The highest prevalence rate was observed in Jinju city (11.2%). 8. Total of 5,005 Parafossarulus sp., the snail intermediate host of C. sinensis, were examined for the detection of cercariae. The cercarial expulsion rate was 0.34%, and the snails collected in Jin-yang-lake side and in the down stream expelled mainly the cercariae of C, sinensis. 9. About 788 cercariaejday (range: 127∼1,503) were expelled daily from a snail naturally infected with C. sinensis. The snails which released more than 1,000 cercariae/day were 30.8% out of total collected. A snail uniquely released 5,840 cercariae/day in this study. 10. The other trematode cercariae besides C. sinensis were also detected, and the rates out of total snails were the cercariae of Losogenes liberum 6.71%, Cyathocotyle orientalis 0.26%, Notocotylus attenuatus 2.52% and Mucobucaris 0.54%, 11. Ten out of 18 species of fresh water fish caught along the river harbored the metacercariae of C, sinensis. The highest rate of metacercarial infection in fish was detected in Pseudorasbora larva (85.9%). The fish mainly eaten by the inhabitants along the Nam-river, and the metacercarial infection rates were: Zacco platypus 8.0%, Hemibarbus sp. 18.2∼26.7%, Gnathepcgcn sp. 37.5%, Ischikauia steenackeri 42.9% and Pseudogobio esocinus 16.7%. 12. Out of 36 P. larva, the number of metacercariae were about 109 (ranEe; 18∼446) per fish and 27 per gram of flesh. The fish caught in spring harbored the highest number of metacercariae. As indicated above, the prevalence of C. sinensis infection in the inhabitants residing around the Nam-river was relatively high. The farther toward the upper stream areas, the higher was the positive rate of C. sinensis infection, and most of clonorchiasis cases were lightly infected. The snail hosts of C. sinensis distributed all around the Nam-river. Several species of freshwater fish were infected with the metacercariae of C. sinensis and the infection rates were relatively high. To prevent C. sinensis infection in the endemic areas, the effective health education system is suggested as a control measure, although mass treatment is also expected to be useful, using chemotherapeutic agents such as "praziquantel", a recently developed anthelminthic for C. sinensis inftraction.

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Effects of Planting Density on Plant Growth and Tuber Yield of Rehmannia glutinosa (지황(地黃) 재식밀도(栽植密度)가 생육(生育) 및 수량(收量)에 미치는 영향(影響))

  • Choi, In-Sik;Song, In-Kyu;Kim, Jun-Ho;Cho, Jin-Tae;Hong, Yu-Ki;Park, Song-Kyu;Park, Jong-Kooi
    • Korean Journal of Medicinal Crop Science
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    • v.1 no.1
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    • pp.70-73
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    • 1993
  • This experiment was carried out to investigate the effect of planting density on thegrowth and yield of Rehmannia glutinosa. A local variety was planted on the 100cm row, with different planting density of 20, 30 and 40 plants per $m^2$. Nitrogen, phosporus and potassium fertilizers were applied as 12, 12, and 16 kg /10a, respectively with compost of 1,000kg /10a. Plant height became shorter with increase in the planting density from 20 to 40 $plants\;/\;m^2$. But emergence date, leaf length and width were not significantly changed with planting density. As the planting density was increased, tuber diameter got slender and tuber number was decreased, but tuber length was not affected. The tuber yield was 793kg /10a at the planting density of 20 $plants\;/\;m^2$ and it increased 40% at 30 $plants\;/\;m^2$ and 45% at 40 $plants\;/\;m^2$ Regarding on the farmer's income, optimum planting density was estimated 30 $plants\;/\;m^2$.

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Study on the Propagation of Acanthopanax Plants II. Characteristics of Seed and Growth of Embryo in Stratifying Treatment (오갈피나무속(屬)(Acanthopanax) 식물의 번식(繁殖)에 관한 연구(硏究) II. 종자형질(種子形質)과 배(胚) 발육(發育)특성)

  • Ahn, Sang-Deuk
    • Korean Journal of Medicinal Crop Science
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    • v.1 no.1
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    • pp.16-23
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    • 1993
  • Seeds of Acanthopanx plants need about 2 years to germinate after sowing even if full matured fruits were harvested. I leave a room for doubt that this phenomenon could be brought by the immatured embryo in seed. If it is true, stratification method for the seed propagation of Acanthopanax plants would be used more effectively to promote the growth of embryo in relatively short time. Before stratification, seeds were devided into two parts. One of them was treated for 24hrs in the concentration of $GA_3$ 100 ppm. After stratification of non-treated and treated seeds, seeds for microtechnique were taken on interval of one month for three months, and fixed in Farmer's solution. The seed sizes of Acanthopanax plants were in biggest order A. sessiliflorus, A. seoulense, A. chiisanensis, A. koreanum and A. sieboldianum. The dehiscence phenomenon of seed coat didn't show in most of the seeds that stratified, but A. koreanum only dehisced in seeds treated in $GA_3$ 100ppm. The embryos of the stratified seeds that treated in $GA_3$ showed nomal growth, complete cotyledons and procambium in hypocotyl in any species, but the non-treated seeds could not expect the germination after sowing due to poor growing of the embryo in all species. In view of the results so far achieved, it has taken long times from sowing to germinate because the seed of Acanthopanax plants has immatured embryo.

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The Landscape of Seonyoo-do Park Captured in One-Person Media Focusing on Blogs (1인 미디어 블로그(Blog)가 포착한 선유도공원 경관)

  • Bark, Sun-Hee;Kim, Yun-Geum
    • Journal of the Korean Institute of Landscape Architecture
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    • v.39 no.3
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    • pp.64-73
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    • 2011
  • This study starts from the hypothesis that the information society has affected the layman's interpretation and production of content. Specifically, the manner and contents of communication concerning the landscape of Soonyoo-do Park in blogs are surveyed and the possibilities and limitations of this phenomenon are discussed. The following topics are dealt with. Firstly, what is the landscape of Seonyoodo Park as captured by bloggers? What type of landscape do bloggers respond to? Secondly, what is the unique way that bloggers capture and interpret the landscape? Thirdly, What are the possibilities and limitations discovered from the landscape as captured and interpreted by bloggers? Thus, 1,000 blog posts concerning Soonyoo-do Park, as culled from the Internet, were categorized into three areas, First are blog posts browsed by keywords such as 'photo', 'a photographer's visit', 'a good p1ace for taking photos', and 'landscape'. These are focused on the visual aspects of the landscape. The second category is posts under the keywords 'domestic travel', 'Seoul travel', 'travel', and 'recommendation'. They contain introductory information on Seonyoodo Park; that is, they focus cm the more utilitarian functions of Seonyoodo Park as a place. The third one is posts that record personal experiences. The subjects for photography are the bloggers themselves and their companions. As a result of studying the way bloggers deal with landscape, it was found that first, people have developed the ability to capture the landscape and interpret the landscape actively and independently. This process can be regarded as the reproduction of landscape and place. In addition, the recording of their appreciation and feeling overlaps with evaluation and assumption. One negative aspect, however, is that many bloggers dramatize and repeat similar scenes. This can be seen as a make-up of image. The limitations of this study include difficulty in interpretation because blogs, which are the objects of this study, are very subjective and personal. In addition, it was not easy to categorize posts because there were diverse images and a broad range of writing. Nevertheless, practitioners of landscape architecture should continue to monitor and use one-person media like blogs, because the relationship between modern man and the landscape can be better understood through them.

An Analysis of Determinants of Smoking and Drinking of Community people in Rural area (농촌주민의 흡연 및 음주 실태와 이에 영향을 미치는 요인분석)

  • 남철현
    • Korean Journal of Health Education and Promotion
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    • v.6 no.1
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    • pp.48-59
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    • 1989
  • The study was designed. to analyse the factors effecting to smoking and drinking of community people in rural area. The study has been surveyed through interviews by trained college students for 1,846 residents who live in rural area(Eup or Myun office is located)for 20days(from 3rd. to 22M. in August, 1988) The summary and conclusions are as follows. 1. The number of answers on the questionaires was 1846. And the percent of man was 55.7 %(woman 44.3 %). Among age group 20-29 years group with 30.4 % was larger than any other group. Anaverage age of answers was 38.6 years. 2.In the period of residence, 21.5 % of the residents lived within 5 years(This group was larger than any other group). In occupations, workers in agriculture and fishing was more than other job occupants. In education, the percent of high school graduates was 44.0 %(higher than any other group) 3.The smoking rate of rural residents was 41.8 %and man's smoking rate was 76.0 % (woman's smoking rate was 7.6 %). These rates were higher than the rates in 1985 (The smoking rate of nation was 32.0 %). First of all, woman's smoking rate increased rapidly 4.As for smoking amount, in male 52.8% of smokers smoked a cigarette case (20 cigarettes) in a day, and 16.9 % of smokers smoked more than one cigarette case. In female 42.5 % of woman smokers smoked a half case in a day and the rate of non-smokers diminished to 77.8 % 5.The rate of non-smokers in 21-29 years group was higher than any other group and a smoking rate increased as an age increased. However, a smoking amount decreased as an age increased. This suggests that people are more concerning about their health as their ages increase.. 6.The smoking rate of college graduates was 58.8 %(higher than any other group) and in a smoking amount 36.4 % of college graduates, 29.8 % of high school graduates smoked more than a cigarette case in a day. This shows that people in a higher education group smoke more than those in other groups. 7.As for non-smoking rate, students, service job workers, company employees, and teachers was 54.1%, 43.4%. 40.1% and 39.5%. respectively. As for smoking rate, workers in agriculture and fishing was the highest level of all job employees. Public officers smoked less than the workers in agriculture and the smoking rate of teachers was less than that of public officers. with regard to smoking amount. above one cigarette case in a day was 39.9 % in public officers, 39.2 % workers in agriculture and fishing, 37.9 % in businessmen, 34.2 % in teachers, 31.9 % in service job employees and 31.6 % in a company employees. 8.The variables which had an effect on smoking were sex (B=.1701), job(B=.1688), education(B=.1671), age(B=.1125). These variables were significant in P<0.05 statistically. Explanatory variance level was 19% 9.The drinking rate of community residents was 61.8% and man's drinking rate was 81.7%(woman's drinking rate was 38.9%). As drinking rate 18.0%(the highest rate) of man drinkers drank 3 bottles of beer in a general drinking and 12.1% of them drank more than 10 bottles. 12.1% (the highest rate)of woman drinkers drank 2 cups of beer. The rising rate of woman's drinking of alcohol was remarkable. 10.Each non-drinking rate of age groups was 27.6% (in 20-30 years group), 28.0% (in 30-39 years group), 28.9%(in 40-49 years group) and 32,6%(in 50-59 years group), 10,7%(the highest rate) of 20-29 and 30-39 years groups drank above 3 bottles. 7.5% of 20-29 years group and 7.7% of 30-39 years group drank above 10 bottles. In 40-49 years group, 14.4 % of them drank a bottle and 8.1% of them drank above 10 bottles. In 50-59years group, 14,2% of them drank 2 bottles and 5.3 % of them drank above 10 bottles. This shows that a drinking rate decresed as an age increased. 11.Non-drinking rate was higher as an education level was lower. Each non-drinking rate of non-educated group, elementary school group, middle school group and high school group was 41.0 % of high school graduates and 14.5 % of college graduates drank 3 bottles of beer. 9.7 %(the highest rate) of college graduates drank above 10 bottles, in general drinking. 12.Each non-drinking rate of businessmen, farmers, service job workers, and students was 31.3%, 28.2%, 26.8% and 25.9%. However, Each drinking rate of public officers, company employees and teachers was 73.3 %, 72.2 % and 68.4 %. This tells us that the drinking rate of mental workers is higher than that of physical workers. 14.9 % of farmers and 14.4 % of public officers drank 3 bottles at a time. 10.5 % of teachers and 9.9 % of public officers drank above 10 bottles. 13.The variables which affect on drinking were sex(B=.1545), education(B=.1476), job(B=.1064), and age(B=.1052). These variables were significant in the level of 0.05 % and explanatory variance level was 18 %. 14.Government have to educate people and demonstrate the health hazards caused by smoking and over-drinking. Especially, No-smoking campaign for woman and education not to over drink for professional job workers are necessary.

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Effect of Small Black Soybean Powder on Blood Glucose and Insulin Sensitivity in Streptozotocin-Induced Diabetic Rats (Streptozotocin-유도 당뇨모델을 이용한 쥐눈이콩 분말의 혈당강하 및 인슐린 감수성의 효과)

  • Lee, Dae-Hoon;Kwak, Dong-Hoon;Kim, Sung-Min;Ju, Eun-Jin;Choi, Han-Gil;Kim, Ok-Hee;Hwang, Jin-Bong;Bae, Nahm-Gung;Jung, Kyu-Yong;Han, Jin-Chul;Park, Hum-Dai;Choo, Young-Kug
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.10
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    • pp.1618-1625
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    • 2004
  • Beans are acknowledged to be food resources, which have more abundant proteins and fats. The constituent parts of beans (i.e. aspartic aid, glycine, arginine) are effective against diabetes, and dietary fiber contained in the beans has an important property to maintain insulin sensitivity. Based on these, using streptozotocin (STZ)-induced diabetic rats, this study examined how the rat-eye soybean, which is principal products of the Imsil province, is effective to attenuate and/or prevent the development of diabetes mellitus. We divided rats into the non-diabetic and diabetic group, and diabetic group was further subdivided into six experimental groups [DC, diabetic control; DI, diabetes with insulin treatment (4∼6 IU/rat); DB, diabetes with black bean; DY, diabetes with yellow soybean; DS, diabetes with rat-eye soybean; DSS, diabetes with vinegar-fermented rat-eye soybean. All bean treatment (1.5 mg/l g body weight).]. Food efficiency ratio (FER), body weight and insulin sensitivity in diabetic rats were significantly reduced compared to those in normal control animals. These reductions were obviously attenuated by administration of a variety of beans used in this study (20∼30%), and the recovery effects were comparable to the results obtained by insulin treatment. Taken together, this study suggests that all beans used may have an essential property to improve and/or attenuate the development of diabetes mellitus in rats.

Effect of Sucrose Concentration on Survival After Frozen-thawed of Bovine IVF Blastocysts in Ethylene Glycol Based Freezing Medium for Slow-Cooling (소 체외수정란의 Slow Freezing을 위해서 Ethylene Glycol 동결보호제에 Sucrose 첨가 농도에 의한 동결효율)

  • 조상래;김현종;최창용;진현주;손동수;최선호
    • Journal of Animal Science and Technology
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    • v.48 no.6
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    • pp.797-804
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    • 2006
  • The present study was undertaken to investigate the post-thawed survivability of bovine embryo depending on different dose of ethylene glycol and sucrose. Ovaries were collected at local slaughterhouse and the cumulus-oocyte-complexes aspirated from ovaries were in vitro matured, fertilized and cultured at 39°C in an atmosphere of 5% CO2 incubator. For conventional slow-freezing, d 7 or 8 expanded blastocysts were collected. Embryos were equilibrated in 1.5 M and 1.8 M ethylene glycol(EG) with 0.1 M and 0.3 M sucrose in Dulbecco's phosphate-buffered saline(D-PBS) supplemented with 0.5% bovine serum albumin. Embryos were then loaded individually into 0.25ml-straw and placed directly into cooling chamber of programmable freezer precooled to 󰠏7°C, after 2 min, the straw was seeded, maintained at 󰠏7°C for 8 min, and then cooled to 󰠏35°C at 0.3°C/min, plunged and stored in liquid nitrogen for at least 3 days. For thawing, the straw containing embryos were warmed in air for 10 sec and exposed to 37°C water for 20 sec. Straws were then removed from 37°C water. Rates of blastocyst survive and hatching were evaluated at 24 to 72 h post-warming. No difference of the survivability was shown between 1.5 M and 1.8 M EG (71 and 70%, respectively). Addition of 0.1 M sucrose to 1.5 M and 1.8 M ethylene glycol in the freezing solution did not differ significantly embryo survival (74 and 77%, respectively), whereas survival rates was higher(89%) in freezing solution contained 0.3M sucrose to 1.8M EG compared with 0.3M sucrose to 1.5M EG group(71%). However, there was no difference in the overall total cell number between the two groups (122±1.8 vs 131±1.4, respectively). In conclusion, the results suggest that 0.3 M sucrose in 1.8 M EG may be optimal condition for freezing and thawing methods with in vitro produced embryos and may be applied to on-farm conditions for embryo transfer.