• 환경위생공학
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• 제14권4호
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• pp.143-149
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• 1999

In the tungsten industry as light source material, tungsten filament which used as light source material could form after molybdenumwire which used as the center supporter for coil shape tungsten wire was removed. This process uses hydrogen peroxide, Ozone and UV(Ultraviolet)Lamp, for the quantity of hydrogen peroxide decrease. The results were as follows : 1. An incandescent electric Lamp type : FL(FL-20) type : A standard of commodity (P.W.: $19{\pm}1.0mg$, $C.R:4.5{\pm}0.3{\Omega}$) 1) Only hydrogen peroxide treated ; Reaction Time : 65Min., P.W.: 18.60mg, $C.R.:4.60{\Omega}$ 2) Ozone/Ultraviolet/70% of hydrogen peroxide; Reaction Time : 64Min., P.W.: 18.61mg, C.R.: $4.61{\Omega}$ 2. A Fluorescent Lamp Type : GLS(GLS-40) Type : A standard of commodity(P.W.: $11.8{\pm}0.2mg$$65{\pm}1.5{\Omega}$) 1) Only hydrogen peroxide treated ; Reaction Time: 72Min, P.W.:11.88mg, C.R.: $65.62\Omega$ 2) Ozone/Ultraviolet/70% of hydrogen peroxide;Reaction Time:71Min., P.W.:11.88mg, C.R.: $65.63\Omega$

• 한국식품과학회지
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• 제23권4호
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• pp.452-458
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• 1991

체다치즈 숙성기간 중 존재하는 저온성세균을 분리하여 단백질분해능이 우수한 균주를 선발하였고 이 선발된 균주가 생산하는 효소의 특성을 연구하였다. 체다치즈 숙성기간 중 미생물의 변화를 관찰한 결과 내냉성세균이 일정하게 유지되고 있었는데 그 중 200개의 균주를 1차 선발한 후 단백질분해능이 우수한 균주 3개를 분리, 동정한 결과 P. fluorescens 두 종과 A. denitrificans였다. 그 중 활력이 가장 높은 P. fluorescens 65가 생산하는 효소를 gel filtration에 의해 정제한 결과 $190{\sim}230ml$ elution volume에서 protease가 용출되었고 SDS-PAGE로 분석한 결과 분자량은 47,000인 것으로 나타났으며 아미노산 조성은 Glu(14.96%)와 Ser(13.83%)의 함량이 제일 높았고 Met, Trp은 존재하지 않았다. P. fluorescens 65의 성장곡선에 따른 효소활력을 실험한 결과 세포증식이 활발한 대수증식기에서 단백질분해효소를 많이 분비함이 나타났으며 pH는 변화가 없이 일정하였다. Extracellular protease 65의 반응 최적온도는 $45{\sim}50^{\circ}C$ 사이었으며 최적 pH는 6.0으로 나타났다.

• Korean Chemical Engineering Research
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• 제53권3호
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• pp.391-396
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• 2015

n-부텐의 산화탈수소화에서 제조방법이 촉매의 반응활성에 미치는 영향을 조사하기 위하여 $BiFe_{0.65}MoP_{0.1}$ 산화물 촉매를 모델 촉매로 선정하여 공침법, 시트르산법, 수열합성법, 주형법 등의 방법으로 촉매를 제조하였다. 제조한 촉매의 물리 화학적 특성을 알아보고 반응 활성과 연관시키기 위하여 X-선 회절분석(XRD), 질소 흡착 탈착분석($N_2$ sorption), 암모니아/1-부텐-승온탈착분석($NH_3/1$-butene-TPD) 등의 특성분석을 수행하였다. 공침법으로 제조한 촉매의 활성이 가장 높게 관찰되었으며, 14시간 동안의 산화탈수소화 반응 기준으로 n-부텐의 전환율은 79.5%, 1,3-부타디엔의 선택도는 85.1%, 1,3-부타디엔 수율은 67.7%의 수치를 보였다. 암모니아 승온탈착 실험으로부터 촉매의 반응 활성은 촉매의 산특성과 밀접하게 관련이 있으며, 공침법으로 제조한 산화물 촉매가 다른 합성방법으로 제조한 촉매와 비교하여 가장 큰 산량을 갖는 것으로 관찰되었다. 또한, 1-부텐의 승온탈착 분석결과, 촉매의 활성은 흡착된 1-부텐과 촉매의 표면반응에 기인한 중간체의 흡 탈착 특성, 즉 약하게 흡착된 중간체(< $200^{\circ}C$)의 상대적인 양과 강하게 흡착된 중간체의 탈착 온도(> $200^{\circ}C$)와 밀접하게 관련이 있었다.

• Molecules and Cells
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• 제40권7호
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• pp.476-484
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• 2017

C-X-C chemokine receptor 4 (CXCR4) stimulates cancer metastasis. NF-${\kappa}B$ regulates CXCR4 expression in cancer cells, and O-GlcNAc modification of NF-${\kappa}B$ promotes its transcriptional activity. Here, we determined whether CXCR4 expression is affected by O-GlcNAcylation of NF-${\kappa}B$ in lung metastasis of cervical cancer. We found elevated levels of O-linked-N-actylglucosamine transferase (OGT) and O-GlcNAcylation in cervical cancer cells compared to those in non-malignant epithelial cells and detected increased expression of NF-${\kappa}B$ p65 (p65) and CXCR4 in cervical cancer cells. Knockdown of OGT inhibited the O-GlcNAcylation of p65 and decreased CXCR4 expression levels in HeLa cells. Thiamet G treatment increased O-GlcNAcylated p65, which subsequently enhanced CXCR4 expression levels. Inhibition of O-GlcNAcylation by 6-Diazo-5-oxo-L-norleucine (DON) treatment decreased p65 activation, eventually inhibiting CXCR4 expression in HeLa cells. Lung tissues from mice engrafted with OGT-knockdown HeLa cells (shOGT) exhibited lower expression of Ki-67 and HPV E6 and E7 oncogenes compared to lung tissues from mice engrafted with control HeLa cells (shCTL). In addition, lung tissues from mice engrafted with shOGT cells exhibited lower p65 and CXCR4 immunoreactivity compared to tissues from mice engrafted with shCTL cells. Taken together, our data suggest that p65 O-GlcNAcylation promotes lung metastasis of cervical cancer cells by activating CXCR4 expression.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.2379-2383
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• 2012

Objective: To investigate possible signal pathway involvement in multi-drug resistant P-glycoprotein (P-gp) expression induced by cyclooxygenase-2 (COX-2) in a human gastric adenocarcinoma cell line stimulated with pacliaxel (TAX). Methods: The effects of TAX on SGC7901 cell growth with different doses was assessed by MTT assay, along with the effects of the COX-2 selective inhibitor NS-398 and the nuclear factor-KB (NF-KB) pathway inhibitor pyrrolidine dithiocarbamate (PDTC). Influence on COX-2, NF-KB p65 and P-gp expression was determined by Western blotting. Results: TAX, NS-398 and PDTC all reduced SGC7901 growth, with dosedependence. With increasing dose of TAX, the expression of COX-2, p65 and P-gp showed rising trends, this being reversed by NS-398. PDTC also caused decrease in expression of p65 and P-gp over time. Conclusion: COX-2 may induce the expression of P-gp in SGC7901 cell line via the NF-kappa B pathway with pacliaxel stimulation.

• Journal of Microbiology and Biotechnology
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• 제13권5호
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• pp.680-686
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• 2003

This study was carried out to characterize the antilisterial substances produced by Leuconostoc sp. W65 and to evaluate the effects of pH, temperature, and time on inhibitory activity using response surface methodology. Leucocin W65, an antilisterial substance produced by Leuconostoc sp. W65, markedly inhibited the growth of Listeria monocytogenes, L. innocua, and L. ivanovii, whereas other pathogens including Gram-negative bacteria were not susceptible. The pH was the most effective factor with regard to bacteriocin activity, while temperature and time of heat treatment had no significant effect. Fifty percent of inhibitory activity remained after 22.8 min at pH 4.2 and $121^{\circ}C$. Leucocin W65 was purified by ammonium sulfate precipitation, hydrophobic interaction chromatography, and tricine-SDS-PAGE. Compositional analysis originally estimated the peptide to be 56 amino acids in length without asparagine, glutamine, and tryptophane. The sequence of partial N-terminal amino acid residues of purified bacteriocin was identified as follows: $NH_{2}-XGXAGVXPXGGQQPXVPLXYP$.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.65-65
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• 2019

In this study, we evaluated the anti-inflammatory effect of extracts of leaves (LCLE) and branches (LCBE) from L. caerulea in LPS-stimulated RAW264.7 cells. Inhibitory effect of LCLE and LCBE against LPS-induced overproduction of NO, iNOS and $IL-1{\beta}$ was higher than LCFE. Furthermore, LCLE and LCBE significantly inhibited the overexpression of COX-2, IL-6 and $TNF-{\alpha}$ in LPS-stimulated RAW264.7 cells. LCLE and LCBE did not inhibited LPS-induced degradation of $I{\kappa}B-{\alpha}$, but blocked the nuclear accumulation of p65. LCLE did not inhibited LPS-induced phosphorylation of ERK1/2 and p38, while LCBE significantly attenuated phosphorylation level of p38. LCLE and LCBE increased HO-1 protein level and decrease of iNOS and $IL-1{\beta}$ expression by LCLE and LCBE was inhibited by HO-1 knockdown. The inhibition of p38 by SB203580 and ROS by NAC blocked HO-1 expression by LCLE and LCBE. LCLE and LCBE increased p38 phosphorylation and the inhibition of ROS by NAC blocked p38 phosphorylation LCLE and LCBE. LCLE and LCBE induced nuclear accumulation of Nrf2, but this was significantly reversed by the inhibition of p38 and ROS. In addition, LCLE and LCBE increased ATF3 expression and decrease of iNOS and $IL-1{\beta}$ expression by LCLE and LCBE was inhibited by ATF3 knockdown. Collectively, LCLE and LCBE inhibited LPS-induced $NF-{\kappa}B$ activation by blocking p65 nuclear accumulation, increased HO-1 expression by ROS/p38/Nrf2 activation, and increased ATF3 expression. Furthermore, LCBE inhibited LPS-induced p38 phosphorylation.

• 한국임상수의학회지
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• 제28권2호
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• pp.190-195
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• 2011

본 연구에서 돼지 PBMC에 t10c12-CLA 처리는 TNF-${\alpha}$생산을 증가시켰으나, LPS 자극 PBMC에서는 TNF-${\alpha}$생산을 감소시켰다. t10c12-CLA 처리는 PBMC의 inhibitory ${\kappa}B$ ($I{\kappa}B$)-${\alpha}$ 단백질 분해를 증가시키고 NF-${\kappa}B$ p65 활성 수준을 증가시켰다. 그러나 LPS 자극 PBMC에서는 상반되는 효과가 관찰되었다. 특히, LPS 비자극 PBMC에서 t10c12-CLA는 NF-${\kappa}B$ 저해제인 caffeic acid phenethyl ester (CAPE)를 처리한 경우 NF-${\kappa}B$ p65 활성 수준을 증가시켰으나 반대로 LPS로 자극한 CAPE 처리 PBMC에서는 NF-${\kappa}B$ p65 활성 수준을 억제시켰다. 이상의 결과는 t10c12-CLA가 돼지 PBMC에 있어 LPS 자극 유무에 따라 다른 효과를 가질 수 있으며, 이는 NF-${\kappa}B$ p65 활성도의 변화와 관련성이 있음을 보여주고 있다.

• Korean Chemical Engineering Research
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• 제53권6호
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• pp.824-830
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• 2015

1-부텐의 산화탈수소화에서 다양한 인 전구체가 촉매의 반응활성에 미치는 영향을 조사하기 위하여 $BiFe_{0.65}MoP_{0.1}$ 산화물 촉매를 모델 촉매로 선정하여 인산수소암모늄, 인산수소이암모늄, 인산, 트리에틸인산, 오산화인 등의 인 전구체를 사용하어 촉매를 제조하고 산화탈수소화 반응을 수행하였다. 제조한 촉매의 물리 화학적 특성을 알아보기 위하여 X-선 회절분석(XRD), 질소 흡착 탈착분석($N_2$ sorption), 원소분석(ICP), 전자주사현미경(SEM), 승온재산화분석(TPRD) 등의 특성분석을 수행하였다. 제조한 촉매의 물리적 특성은 인 전구체에 따른 큰 차이는 관찰되지 않았지만 산화탈수소화 반응에서 촉매의 활성은 사용된 인 전구체의 특성에 따라 다르게 관찰되었다. 인산을 전구체로 사용하여 제조한 $BiFe_{0.65}MoP_{0.1}$ 산화물 촉매가 사용된 촉매 중에서 가장 우수한 활성을 나타내었으며, 14시간 동안의 산화탈수소화 반응기준으로 n-부텐의 전환율은 79.5%, 1,3-부타디엔 수율은 67.7%의 수치를 보였다. 인 전구체의 양이온의 특성에 따라 촉매의 격자 구조가 영향을 받는 것으로 추측되며, 이러한 격자 구조의 차이는 촉매의 재산화 능력에 영향을 주는 것으로 사료된다. 환원 처리된 촉매의 승온재산화 실험으로부터 촉매의 반응활성은 촉매의 재산화 능력과 밀접하게 관련이 있었으며, 인산을 전구체로 사용하여 제조한 산화물 촉매가 다른 인 전구체와 비교하여 가장 좋은 재산화 능력을 나타내었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8579-8587
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• 2016

Background: The molecular mechanisms linking breast cancer progression and inflammation still remain obscure. The aim of the present study was to investigate the possible association of angiopoeitin like protein 4 (ANGPTL4) and its regulatory factor, hypoxia inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$), with the inflammatory markers nuclear factor kappa B/p65 (NF-${\kappa}B$/P65) and interleukin-1 beta (IL-$1{\beta}$) in order to evaluate their role in inflammation associated breast cancer progression. Materials and Methods: Angiopoietin-like protein 4 (ANGPTL4) mRNA expressions were evaluated using quantitative real time PCR and its protein expression by immunohistochemistry. DNA binding activity of NF-${\kappa}B$/P65 was evaluated by transcription factor binding immunoassay. Serum levels of ANGPTL4, HIF-$1{\alpha}$ and IL-$1{\beta}$ were immunoassayed. Tumor clinico-pathological features were investigated. Results: ANGPTL4 mRNA expressions and serum levels were significantly higher in high grade breast carcinoma ($1.47{\pm}0.31$ and $184.98{\pm}18.18$, respectively) compared to low grade carcinoma ($1.21{\pm}0.32$ and $171.76{\pm}7.58$, respectively) and controls ($0.70{\pm}0.02$ and $65.34{\pm}6.41$, respectively), (p<0.05). Also, ANGPTL4 high/moderate protein expression was positively correlated with tumor clinico-pathological features. In addition, serum levels of HIF-$1{\alpha}$ and IL-$1{\beta}$ as well as NF-${\kappa}B$/P65 DNA binding activity were significantly higher in high grade breast carcinoma ($148.54{\pm}14.20$, $0.79{\pm}0.03$ and $247.13{\pm}44.35$ respectively) than their values in low grade carcinoma ( $139.14{\pm}5.83$, $0.34{\pm}0.02$ and $184.23{\pm}37.75$, respectively) and controls ($33.95{\pm}3.11$, $0.11{\pm}0.02$ and $7.83{\pm}0.92$, respectively), (p<0.001). Conclusion: ANGPTL4 high serum levels and tissue expressions in advanced grade breast cancer, in addition to its positive correlation with tumor clinico-pathological features and HIF-$1{\alpha}$ could highlight its role as one of the signaling factors involved in breast cancer progression. Moreover, novel correlations were found between ANGPTL4 and the inflammatory markers, IL-$1{\beta}$ and NF-${\kappa}B$/p65, in breast cancer, which may emphasize the utility of these markers as potential tools for understanding interactions for axes of carcinogenesis and inflammation contributed for cancer progression. It is thus hoped that the findings reported here would assist in the development of new breast cancer management strategies that would promote patients' quality of life and ultimately improve clinical outcomes. However, large-scale studies are needed to verify these results.