• Journal of Environmental Health Sciences
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• v.9 no.1
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• pp.89-93
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• 1983

Various analytical methods for p-Nitrophenol derivatives have been reported as follows. 1) Thin-Layer Chromatography, 2) Gas Chromatography, 3) Cholinesterase Activity Determination, 4) Diazo Method, 5) Nitrophenol Method, 6) Indophenol Method. But these methods are mainly analyse total quantity of p-Nitrophenol and are not available for the seperation and pose some analytical problems associated with extensive clean up procedure. A rapid and simple method was developed for the seperation and quantitation for the p-Nitrophenol and it's derivatives by HPLC. Also an experiment was undertaken by the authors for the quantitation of the p-Nitrophenol in the blood of the intoxicated body. Levels of p-Nitrophenol ranging from approximately 0.10 to $1.69 \mu g/ml$ for Parathion and $3.44 \mu g/ml$ for EPN in each sample were measured with the average recovery of $95.5\pm0.52%$

• Membrane and Water Treatment
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• v.13 no.2
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• pp.97-104
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• 2022

With a small particle size, specific surface area and chemical nature, Pd/Cu-Fe nanocomposites can efficiently remove the organic compounds. In order to understand the applicability for in situ remediation of contaminated groundwater, the degradation of p-nitrophenol by Pd/Cu-Fe nanoparticles was investigated. The degradation results demonstrated that these nanoparticles could effectively degrade p-nitrophenol and near 90% of degradation efficiency was achieved by Pd/Cu-Fe nanocomposites for 120 min treatment. The efficiency of degradation increased significantly when the Pd content increased from 0.05 wt.% and 0.10 wt.% to 0.20 wt.%. Meanwhile, the removal percentage of p-nitrophenol increased from 75.4% and 81.7% to 89.2% within 120 min. Studies on the kinetics of p-nitrophenol that reacts with Pd/Cu-Fe nanocomposites implied that their behaviors followed the pseudo-first-order kinetics. Furthermore, the batch experiment data suggested that some factors, including Pd/Cu-Fe availability, temperature, pH, different ions (SO42-, PO43-, NO3-) and humic acid content in water, also have significant impacts on p-nitrophenol degradation efficiency. The recyclability of the material was evaluated. The results showed that the Pd/Cu-Fe nanoparticles have good recycle performance, and after three cycles, the removal rate of p-nitrophenol is still more than 83%.

• Bulletin of the Korean Chemical Society
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• v.29 no.5
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• pp.985-987
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• 2008

Parathion is an organophosphate pesticide being legally applied for the purpose of agriculture and is being manufactured in Korea. A gas chromatography/mass spectrometric method was developed for the determination of parathion urinary metabolite, p-nitrophenol. p-Nitrophenol was extracted from weak acidic urine, and then measured by gas chromatography-mass spectrometry (selected ion monitoring). The recovery of pnitrophenol in the overall procedure was 88.2%. The detection limit of the assay was 1.0 $\mu$ g/L based upon assayed urine of 2.0 mL. The method was applied to the determination of p-nitrophenol in urine of workers of a parathion industry. Spot urines of workers of a parathion industry were sampled at the end of shift and pnitrophenol was analyzed using above developed method. p-Nitrophenol could be detected in all of the urine samples at concentrations varying from 3.0 to 681 $\mu$ g/L.

• Analytical Science and Technology
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• v.17 no.1
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• pp.16-22
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• 2004

Inclusion complexes of the p-nitrophenol with ${\beta}$-cyclodextrin (CD), sulfated ${\beta}$-CD, and ${\beta}$-CD polymer cross-linked with epichlorohydrine (EP) were prepared and characterized by Raman spectroscopy. The intensity of vibration peaks of the C-O and C-N at 1284 and $856cm^{-1}$ of the p-nitrophenol in the presence of EP-linked CD polymer was remarkably increased, respectively. The vibration modes at 1284 and $856cm^{-1}$ are assigned to the out-of phase C-C-O stretching mode and the C-N stretching mode, respectively. The vibration peaks at 1284 and $856cm^{-1}$ increased with increasing the content of EP-linked CD polymers, while decreased with increasing the p-nitrophenol content. Furthermore, the vibration mode of the $NO_2$ symmetric stretch at $1344cm^{-1}$ enhanced with increasing the content of p-nitrophenol.

• Journal of Radiation Industry
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• v.5 no.4
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• pp.353-357
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• 2011

Degradation of p-nitrophenol has been carried out using only gamma irradiation or gamma irradiation with $H_2O_2$ or $Na_2S_2O_8$. Effects of different operating parameters such as initial concentration ($50mg\;l^{-1}$, $100mg\;l^{-1}$, $200mg\;l^{-1}$, $300mg\;l^{-1}$, $400mg\;l^{-1}$, $500mg\;l^{-1}$ and $600mg\;l^{-1}$) on the extent of degradation has been investigated. At 5 kGy, $50mg\;l^{-1}$ p-nitrophenol was completely degraded, and the radiolytic degradation of p-nitrophenol was described by the pseudo-first-order kinetic model. The combination of gamma irradiation with $H_2O_2$ or $Na_2S_2O_8$ leads to an enhanced effect, which remarkably increased the degradation efficiency of p-nitrophenol and TOC removal. However, at high $H_2O_2$ concentration, the efficacy of p-nitrophenol degradation is reduced because ${\cdot}OH$ radicals are scavenged by $H_2O_2$ and $Na_2S_2O_8$.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.17 no.4
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• pp.300-309
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• 2007

Although parathion is an organophosphate pesticide being legally applied for the purpose of agriculture and is being manufactured, parathion in the air and urinary p-nitrophenol, a metabolite of parathion, were not analysed in Korea. Air of the parathion manufacturing workplace was sampled by OVS-2 tubes using NIOSH 5600 and spot urine of workers was sampled at the end of shift. Parathion and urinary p-nitrophenol were analysed by GC/MS (5973 MSD connected with Agilent 6890 GC) and the protocol was included in this study. It was found that this protocol should be so sensitive that determining parathion in the air and urinary p-nitrophenol below level of ACGIH TLV and BEI be adequate. Another finding was that total sampling volume of air of NIOSH 5600 of 240 L should be adjusted to be less than 120 L due to breakthrough.

• Journal of Preventive Medicine and Public Health
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• v.25 no.2 s.38
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• pp.180-188
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• 1992

The effects of multiple exposures to pesticides on plasma cholinesterase(ChE) activities and urinary p-nitrophenol excretion were evaluated in rats. Rats were received single dose i.p. with $LD_{50}/100(mg/kg)$ of organophosphorous(OP), organophosphorous-organochroline(OP-OC), organophosphorous-carbamate(OP-CAB), organophosphorous-organoarsenate(OP-OA) pesticides for 4 consecutive days. In repeated administration of pesticides, plasma ChE activities were decreased, but urinary p-nitrophenol were increased after the first injection and then decreased gradually The recovery rates of ChE activities and p-nitrophenol excretion at 48 hours after the fourth Injection were delayed in comparision with the baseline value of 24 hours before the first injection. Statistical significances were found between OP and other groups except OP-OA group after the second injection in plasma ChE activities, but in urinary p-nitrophenol excretion there was statistical significance only between OP and OP-CAB.

• Journal of Preventive Medicine and Public Health
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• v.7 no.1
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• pp.115-121
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• 1974

In order to evaluate the health hazard due to exposure to organophosphorus insecticides, we measured the blood cholinesterase activity ana urinary para-nitrophenol among 56 exposed subjects. They are orchard workers, rice plant workers and smithion factory workers. The clinical symptoms were also checked by physicians. We also measured the blood cholinesterase activity and urinary para-nitrophenol excretion of 20 urban people and 15 rural people who had never been exposed to organophosphorus insecticides in order to compare them according to age, sex and geographical differences. And these results were also compared with those of exposed groups. The results obtained were as follows. 1. The normal plasma cholinesterase activity and cell cholinestrase activity were $0.861{\pm}0.148\;{\Delta}pH/hr$ and $0.822{\pm}0.154\;{\Delta}pH/hr$. And normal para-nitrophenol in urine was $1.21{\pm}0.52mg/liter$. 2. No significant difference was existed in blood cholinesterase activities and urinary para-nitrophenol excre tion according to sex, age and geographical difference. 3. The plasma cholinesterase activity and cell cholinesterase activity of orchard workers, rice plant workers and smithion factory workers were $0.682{\pm}0.189\;{\Delta}pH/hr,\;0.775{\pm}0.160\;{\Delta}pH/hr,\;0.754{\pm}0.123\;{\Delta}pH/hr,\;and\;0.691{\pm}0.082\;{\Delta}pH/hr,\;0.756{\pm}0.117\;{\Delta}pH/hr,\;0.739{\pm}0.117\;{\Delta}pH/hr$. And significant decreses in blood cholinesterase activities were existed among orchard workers and smithion factory workers compared with control group. 4, The urinary para-nitrophenol excretions of orchard workers, rice plant workers and smithion factory workers were $1.33{\pm}0.66mg/liter,\;1.19{\pm}0.88mg/liter\;and\;1.37{\pm}0.67mg/liter$ and there were no significant difference between exposed groups and control group. 5. The clinical symptoms complained during and after organophosphorus insecticides exposure were frequently ranked by headache (67.7%) and vertigo (64.5%) and muscular ataxia and weakness (51.6%).

• Analytical Science and Technology
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• v.9 no.3
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• pp.235-243
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• 1996

A carbon paste electrode were chemically modified using ${\alpha}$-cyclodextrin. Characteristics of chemically modified carbon paste electrodes were studied on the basis of the inclusion complex formation of ${\alpha}$-cyclodextrin and p-nitrophenol in solution. Cyclic voltammetry and differential pulse voltammetry were used to monitor the efficiency of the chemical modification. When the ${\alpha}$-cyclodextrin and carbon powder ratio of 2 : 1 in weight were used, the reduction peak current of p-nitrophenol was decreased almost completely, whereas those of o-nitrophenol and hydroquinone were not changed much. This result is due to the large difference in the inclusion complex formation constants of p-nitrophenol and the other probes with ${\alpha}$-cyclodextrin. Taking advantage of this difference, we can determine the concentration of o-nitrophenol even in the presence of p-nitrophenol.

• Applied Chemistry for Engineering
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• v.19 no.3
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• pp.351-356
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• 2008

Titania nanoparticles were prepared by controlled hydrolysis of titanium tetraisopropoxide (TTIP) in water-in-oil (W/O) and microemulsion stabilized with a nonionic surfactant, N P-10 (Polyoxyethylene Nonylphenol Ether: $C_9H_{19}C_6H_4(OCH_2CH_2)_{10}OH$)). The nanosized particles prepared in W/O microemulsion were characterized by FT-IR, TEM, XRD, TGA, and DTA. In addition, the photocatalytic degradation of p-nitrophenol has been studied by using a batch reactor in the presence of UV light in order to compare the photocatalytic activity of prepared nanosized titania. The nanaosized titania particles calcined at $300{\sim}600^{\circ}C$ showed an anatase structure, but it transformed to a rutile phase above $700^{\circ}C$ of calacination temperature. With an increase of $W_o$ ratio, the crystallite size increased but photocalytic activity decreased. The titania synthesized at $W_o=5$, R = 2, and calcined at $400{\sim}500^{\circ}C$ showed the highest activity on the photocatalytic degradation of p-nitrophenol.