• 제목/요약/키워드: p-center problem

검색결과 347건 처리시간 0.03초

A Rapid and Simple Method for Construction and Expression of a Synthetic Human Growth Hormone Gene in Escherichia coli

  • Roytrakul, Sittiruk;Eurwilaichitr, Lily;Suprasongsin, Chittiwat;Panyim, Sakol
    • BMB Reports
    • /
    • 제34권6호
    • /
    • pp.502-508
    • /
    • 2001
  • A cDNA, encoding the human growth hormone (hGH), was synthesized based on the known 191 amino acid sequence. Its codon usage was optimized for a high level expression in Escherichia coli. Unique restriction sites were incorporated throughout the gene to facilitate mutagenesis in further studies. To minimize an initiation translation problem, a 624-bp cassette that contained a ribosome binding site and a start codon were fused to the hGH-coding sequence that was flanked between the EcoRI and HindIII sites. The whole fragment was synthesized by an overlapped extension of eight long synthetic oligonucleotides. The four-short duplexes of DNA, which were first formed by annealing and filling-in with a Klenow fragment, were assembled to form a complete hGH gene. The hGH was cloned and expressed successfully using a pET17b plasmid that contained the T7 promoter. Recombinant hGH yielded as much as 20% of the total cellular proteins. However, the majority of the protein was in the form of insoluble inclusion bodies. N-terminal amino acid sequencing also showed that the hGH produced in E. coli contained formyl-methionine. This study provides a useful model for synthesis of the gene of interest and production of recombinant proteins in E. coli.

  • PDF

초고속 영구자석 동기기의 기초자기회로설계 (Initial Magnetic-Circuit Design of High Speed Permanent-Magnet Synchronous Machine)

  • 주대석;홍도관;우병철;우경일;박한석
    • 전기학회논문지P
    • /
    • 제64권1호
    • /
    • pp.7-13
    • /
    • 2015
  • This paper presents mathematical models for high speed permanent-magnet synchronous machine. The mathematical method with two successive steps is used to estimate design parameter as well as the output power. At first, mathematical model for a linkage flux problem is employed to calculate the number of winding turns and stack length of armature core. The magnetic circuit model for an induced voltage and the electric circuit model for a current are modeled. The output powers of the electrical generator were evaluated by the mathematical techniques. The results of this mathematical methods predict the specifications of the machine and can be applied in the design stage of the electrical machine.

계통(系統)의 안전성(安全性) 제어(制御)를 고려(考慮)한 동적(動的) 전압안정도(電壓安定度)에 관(關)한 연구(硏究) (A Study on the Dynamic Voltage Stability Considering the Power System Security Control)

  • 이근준;황종영;이길순;정태호;김건중;김용배
    • 대한전기학회:학술대회논문집
    • /
    • 대한전기학회 1994년도 추계학술대회 논문집 학회본부
    • /
    • pp.54-60
    • /
    • 1994
  • The cause of black out of Tokyo Power in 1987 has been identified as the voltage stability problem. After this event many researchers has been interested in voltage stability or voltage collapse phenomena. The voltage instability is different Com the transient stability in the sense of reactive power mismatch and the long duration time. In this study, we developed efficient tool for analyze and control the dynamic voltage instability. To analize specific condition of dynamic voltage stability, quasi-dynamic simulation method is developed. To provide proper mathmatical model for dynamic voltage stability, generator, SVC, OLTC, induction motor models are introducted. To provide specified dynamic voltage stability, the authors considered to use reactive loss function(${\partial}Q/{\partial}p_L$) as reactive power facility control index. This program was tested and identified its usefulness in real KEPCO system.

  • PDF

설문조사를 통한 생체 신장 공여자의 기증 후 건강상태와 건강증진 생활방식 (Health Status and Health-promoting Lifestyle for Living Donors after Kidney Donation Through Survey)

  • 남민경;이두인;권오정
    • 대한이식학회지
    • /
    • 제28권3호
    • /
    • pp.144-153
    • /
    • 2014
  • Background: Normal renal function and health have been recognized as important factors in living donors after kidney donation. The purpose of this study was to evaluate the health status and health-promoting lifestyle in living donors after kidney donation. Methods: A total of 678 living-kidney donors were counted in our center from January 1990 to December 2011. Only 84 donors agreed to participate in the survey by telephone. We received consent for participation in our survey from 48 donors (57.1%). Data were collected from May to August 2013 using donor characteristics, health status, and Health Promoting Lifestyle Profile I (HPLP-I). Results: The donors were predominantly female (62.5%) and the average age was 48.9±11.8 years, and the average period after nephrectomy was 9.7±5.7 years. The characteristics of donors included ideal body weight (37.5%), overweight (37.5%) in body mass index, and good health status (81.3%). Most donors underwent an annual medical check-up (56.2%), no health problem (81.3%), and no disease (64.6%). However, one patient was treated with dialysis for renal failure due to diabetes. The total average score for HPLP-I was 128.3±13.9. Higher than average scores (116.3±19.1) were observed for the general middle-aged woman. There were statistically significant differences in self-realization and nutrition in subsection of HPLP-I. Self-realization showed a higher score for Christian (F=2.743, P=0.041) and good health (F=3.389, P=0.017). Nutrition showed a higher score for overweight, obesity (F=6.783, P=0.000), and older than 60 (F=3.854, P=0.009). Conclusions: Most living kidney donors were healthy after their donation and had relatively high scores for health-promoting lifestyle. However, one patient had a serious health problem. In addition, younger, longer period after donation, and the rare health examination of donors showed a lower health-promoting lifestyle. Designed and continuous health-care management after transplantation is needed for kidney donors.

일개 국립결핵병원에 입원치료를 받은 폐결핵환자의 임상양태에 관한 연구 - 1995년과 2002년 비교연구 - (Clinical Observational study of Pulmonary Tuberculosis for admitted patients at a National Tuberculosis Hospital - Comparison with the previous results in 1995 -)

  • 박승규;이인희;김병주
    • Tuberculosis and Respiratory Diseases
    • /
    • 제58권4호
    • /
    • pp.392-398
    • /
    • 2005
  • 배 경 : 1995년 제7차 전국 결핵실태조사가 마지막으로 시행된 이 후 2000년 6월 결핵정보감시체계가 도입되었다. 결핵정보감시체계에서는 기존의 전국결핵실태조사에 포함된 균양성 유병율, 약제내성율 등 중요한 역학적 지표를 알 수 없는 한계를 가지고 있다. 본 연구의 목적은 1995년 12월과 2002년도 12월 국립마산결핵병원에 입원치료중인 환자의 임상양태를 조사 비교함으로 우리나라의 다제내성 결핵을 포함한 결핵실태추이의 한 단면을 알아보고자 함이었다. 방 법 : 2002년 12월 현재 국립마산결핵병원에 입원치료 중인 결핵환자 중 NTM으로 최종 확인된 6명을 제외한 331명을 대상으로 임상양태를 조사하고 이를 1995년에 보고된 자료와 비교조사 하였다. 결 과 : 1995년 자료와 비교할 때 평균연령은 3.6세 많았으며, 결핵치료 과거력과 이전 사용 약제 수는 각각 $2.0{\pm}1.7$회, $6.1{\pm}2.3$제에서 $1.7{\pm}1.8$회, $4.6{\pm}3.6$제로 감소하였다(각각 p<0.05, p<0.001). 전체적인 약제내성율은 비슷하였으나(81.0% vs 77.6%), 초회내성율과 초회다제내성율은 각각 10.5%와 2.4%에서 21.4%와 16.5%로 현저히 증가하였다(각각 p=0.012, p=0.001). 또한 1995년도에는 보건소에서 진단을 받은 경우가 65%였으나 2002년도에는 40.5%로 크게 감소하였다(p<0.001). 결 론 : 초회 내성율이 증가하고, 민간의료기관에서 진료하는 환자의 비중이 증가하는 등 우리나라의 결핵관리상황이 이전과 다르게 변해가고 있으므로 이에 대처할 수 있는 민간 및 공공의료기관이 협력할 수 있는 국가결핵관리프로그램의 수정 및 보완이 필요할 것으로 생각된다.

한방건강증진센터 설립에 대한 인식 및 요구조사 (A Need Assessment on Establishment of Oriental Health Promotion Center)

  • 이향련;김귀분;조결자;신혜숙;김광주;문희자;박신애;김윤희;강현숙
    • 동서간호학연구지
    • /
    • 제5권1호
    • /
    • pp.90-101
    • /
    • 2000
  • The study attempts to examine the degree of cognition and demand on health promotion center of oriental nursing. It puts unique nursing intervention using traditional health promotion connected with oriental medicine to practical use for residents' health promotion and prevention of disease. With the study design of cross-sectional descriptive study, 516 residents who live in 26 Dongs, Dongdaemoon-gu were selected. The tool of study consists of 30 questions which the study team made for the degree of cognition and demand on health promotion center of oriental nursing. Cronbach's $\alpha$ in the degree of cognition was .8028. Collection of research data had been done from October 1 to October 30, 1999 with help of Dongdaemoon-gu office after pretest from 20 residents. Collected data were analyzed into the number and percentage in the characteristic of a subject and connected with demand on the establishment of center, the mean and the standard deviation in the degree of cognition and F-tests in the difference of the degree of cognition by characteristics. The results were as follow; 1) The characteristic of subject of this study was male 50.6%, and average age was 38.5 years old and 30-39 years old occupied the highest percentage with 31.6%. The married were 71.8%, over high school graduates was 85.6%, monthly income from 500 thousand won to 2 million won was 86.1%. 50.8% was the type living with parents, children and sibling. 2) When they were sick, the institution which residents used at first was a pharmacy(69.2%) and hospital(27.5%), but oriental medicine hospital was just 1.4%. As for subjective health condition, 82.5% answered over average, and 28.7% answered that they had chronic illness such as arthritis, chronic digestion problem, hypertension and so on. As for information collection on health, mass communication(34.9%) and medically concerned people(28.1%) occupied relatively high rate. Free health diagnosis system(36.8%) and establishment of health promotion center(31.5%) among welfare programs that residents want to enjoy were high ranked. The rate using a special institution for health was 17.8%, and among these institutions, the rate using aerobic exercises, health center(7.0%) and steamed room(5.4%) was high. Besides, other institutions such as breathing at the abdomen, finger-pressure therapy, meridian massage, foot massage, and so on were being used. 3) As the average of the degree of cognition on health promotion center of oriental medicine was 2.92, the degree of cognition was medium. The description, "health promotion center of oriental medicine is necessary for health keeping of healthy people, including people who have a problem in health" showed the highest degree of cognition(3.04, ${\pm}0.64$). 4) As for the intention on using health promotion center of oriental nursing, 61.4% said "yes", "no" was just 1.4%. The services that people relatively high wanted to be served from the center were measures reducing stress(68.0%) (relaxation therapy, meditation, breathing at the abdomen and so on), acupuncture(66.5%), finger-pressure(61.6%), moxibustion(57.6%), meridian massage(44.2%), postpartum care(40.3%) and so on. 5) As for the degree of cognition on the establishment of health promotion center of oriental nursing by characteristics of subject, there was significant difference(F=4.03, p=.046) between male(3.01) and female(2.91). But there was no significant difference by age, marital status, level of educational achievement and monthly income. As the above result, cognition on the establishment of health promotion center of oriental nursing was relatively low because people were not familiar with about the health promotion center of oriental nursing yet. However once the center will be established, the degree of demand on the center will be relatively high. So positive advertisement will be necessary, and the management of useful programs will be also required in order to make people recognize the advantage when they actually will use the center. On the other hand, as the subject of the study consists of many young people of below 30, the health problem came to be low. And in the case of sampling, the study using random sampling that can represent population will be required.

  • PDF

농촌지역 보건소 환경에 있어서의 건강가치관에 관한 일 조사연구 (A Study on Value Orientation of Health of Rural Health Center Milieu)

  • 김순자
    • 대한간호학회지
    • /
    • 제5권1호
    • /
    • pp.17-30
    • /
    • 1975
  • The concept and definition of nursing and her role have been changing in accordance with the socio-cultural factors of the initial society. At present, nursing is conceptualized as a health care profession assisting man to restore, maintain and promote health by providing knowledge, wilt strength and resources through various processes of interaction. Man′s behavior, of individual and group activities for health inclusive, is driven by the initial man′s value orientation. The purpose of this study is to investigate the value orientation regarding health of rural health center milieu in order to give data for; 1. the planning for the delivery of community nursing service, 2. the health education plan at all level of nursing care activities, and 3. the planning of curriculum for nursing education. A hundred opinion leaders among the labor population residing in rural areas (P-group), hundred and six professional nurses at rural health centers (N-group) were indirectly interviewed through questionaries. And ninety five of N-group were interviewed likewise of their perceptions of P-group (NP-group) from July 15, to October 15, 1974. The result is as follows: 1. Maintenance of health is revealed to be the most valued component for man′s happiness in all the three groups. (P-group: 7.30 S. D.=1.31), (N-group :7.84 S. D. =49), and (NP-group : 5.93 S. D. =2.28) 2. The average value score of the maintenance of health revealed significant difference by P〈.001 level between each of the three groups. (Between N-group and P-group : T= -4.07 P and NP ; T=-6.93, N and NP: T=-9.35) 3. Basic health maintenance activities necessary for maintenance and promotion of personal health were moderately valued by all the three groups, P-group ; 3.74 (S. D. =.43) , N-group: 3.52 (S. D. =.34), NP-group: 3.07 (S. D. :.55) Among the 8 categories of basic health maintenance activities, "food intake" was highly valued by P-group (mean value score; 4.00 S. D=.51) , "exercise and rest" and "personal cleanliness" was highly valued by both P-group (4.02, 4.08) and N-group (4.08, 4.22). 4. The mean value score of basic health maintenance activities revealed significant difference by P〈.001 level between each of the three groups (between P-group and N-group: T=-4, 07, N-and NP: T=-6.93, P and NP T=-9.35) 5. Among the 30 questionaries, dynamic activities for health maintenance were more valued in comparison to passive activities in an tile three groups. 6. In N-group, correlation between the value of basic health maintenance activities and the personal health status personal revealed moderate significance. Correlation between the value of basic health maintenance activities and the age revealed low significance. 7. In group, correlation between the value of basic health maintenance activities and; perception of personal health status revealed non significance, between the age and sex revealed low sign affiance, and between the educational status revealed moderate significance. Recommendations are as follows ; 1. The efforts towards alteration of value orientation concerning health in general in community nursing practice de focussed be that of family Planning. 2. In order to prepare professional nurses competent in understanding individual and group, social science and behavioral science be strength ended in planning nursing curriculum. 3. Milieu of nursing experience during nursing education be Planned to begin at simple nursing problem and move towards complex, f. e. home care towards health crisis situation in order to achieve dynamic role mastery.

  • PDF

종합병원 종사자의 강인성, 대응행위 및 조직몰입과의 관계 (A Study of Relationships Hardiness, Coping Behaviors and Organizational Commitment of General Hospital Employees)

  • 황서연;서은희;박선일
    • 간호행정학회지
    • /
    • 제19권2호
    • /
    • pp.196-206
    • /
    • 2013
  • Purpose: This descriptive correlation study was done to identify the hardiness, coping behavior, and organizational commitment of general hospital employees and show how these variables affect increases in role performance and problem solving ability to have a positive influence on organizational harmony. Methods: A quantitative, descriptive research design was used with a sample of 368 employees working in general hospitals in M and C cities. A survey was used to collect the data. Results: The score for perception of hardiness of general hospital employees was 2.85, and coping behavior was 2.40, both out of a possible 4 points, and organizational commitment, 3.03 out of a possible 5 points. There were statistically significant positive correlations between hardiness and coping behavior (r=.33, p<.001), also between hardiness and organizational commitment (r=.51, p<.001), and also between coping behavior and organizational commitment (r=.22, p<.001). Conclusion: The results of the study indicate that hardiness, coping behavior, and organizational commitment in general hospital employees have positive correlations, and thus hospital administrators should explore ways of increase individual employee hardiness and coping behavior, and make efforts to harmonize their organizations by enhancing organizational commitment.

GAITRite 시스템 분석을 통한 퇴행성 슬관절염 환자의 보행특성 연구 (Study on the Gait Characteristics in Knee Osteoarthritis Patients with GAITRite System Analysis)

  • 황보각;김병조;배성수
    • The Journal of Korean Physical Therapy
    • /
    • 제16권1호
    • /
    • pp.183-207
    • /
    • 2004
  • The purpose of this study was to describe and compare the temporo-spatial gait characteristics of healthy elderly people with those osteoarthritis patients. 100 patients reported knee osteoarthritis, diagnosed at the hospital or clinic located in Daegu and Kyungbuk province and 100 normal elderly subjects were participated in this study. Temporal and spatial parameters of gait were analysed for using the computerized GAITRite system. The system integrates specific components of locomotions to provide a single, numerical representation of gait, the Functional Ambulation Performance score. Differences in gait characteristics between the two groups were examined using a correlated t-test and Pearson Correlation(p<.05). Significant differences were observed between the groups for temporal parameters(step time, double support time, stance phase, mean velocity) and spatial parameters(step length, step/extremity ratio)(p<.05). Also there was difference in the functional ambulation performance score between normal elderly subjects and knee osteoarthritis patients(p<.05). Consequently, it may help detect the abnormal gait pattern indicated the main problem in degenerative knee osteoarthritis patients as well as provide data analysing the pathokinesiologic components by comparing normal elderly.

  • PDF

어머니의 자기 지각 성향과 자녀 문제에 대한 지각 성향간의 관계 - MMPI와 KPI-C의 타당도 척도를 중심으로 - (RELATIONSHIP OF MOTHERS' SELF-PERCEPTION WITH PERCEPTION OF CHILDREN'S PROBLEMS - FOCUSING ON VALIDITY SCALES OF MMPI & KPI-C -)

  • 김근향;정유숙;홍성도;김이영;김지혜
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
    • /
    • 제9권2호
    • /
    • pp.174-179
    • /
    • 1998
  • 본 연구의 목적은 어머니의 자기지각 성향과 자녀 문제에 대한 지각 성향간의 관계를 규명하는 것이다. 소아 정신과를 방문한 아동 62명을 대상으로 어머니의 다면적 인성검사(MMPI)의 타당도 척도와 그 어머니가 보고한 자녀의 한국아동인성검사(KPI-C)의 타당도 척도간의 상관을 구하였고 t-test를 통해 KPI-C 세 타당도 척도(T-R, L, F) 각각에서 MMPI 타당도 척도 각각의 상·하위 집단간에 차이가 있는지를 알아 보았다. MMPI의 K 척도와 KPI-C의 거짓말(L) 척도간에 유의한 정적상관, MMPI의 F 척도와 KPI-C의 L 척도간에 유의한 부적상관을 보였다. t-test결과, KPI-C의 L 척도는 MMPI의 F, K 척도의 상 ${\cdot}$ 하위집단간에 유의한 차이를 보였고 KPI-C의 F 척도는 MMPI L 척도의 상 ${\cdot}$ 하위 집단간에 유의한 차이를 보였다. 즉, 자신의 심리적인 문제에 대해 정교한 방어기제를 사용하는 어머니가 자녀의 심리적인 문제에 대해서도 방어적으로 지각하는 성향이 있는 반면, 자신의 문제를 고통스럽고 과장되게 지각하는 어머니는 자녀의 심리적인 문제에 대해서 과장되게 지각하지는 않음이 시사되었다. 또한 자신의 심리적인 문제를 부인하고 단순한 방어기제를 사용하는 어머니는 자녀의 문제에 대해서는 그 정도를 심각하게 지각하지 않음이 드러났다. 따라서 본 연구결과는 MMPI 타당도 척도 중에서 무엇보다도 K 척도가 상승하는 어머니가 자녀의 문제를 과소평가할 가능성이 있다는 것을 감안하여 그러한 어머니가 보고한 자녀문제의 심각도와 내용을 신중하게 평가하여야 할 것을 시사한다.의 효과에 대해 비교했을 때 품행장애가 공존된 집단에서 치료전에 비해서 아동행동조사표의 문제행동목록 중 공격성 요인에서 유의한 감소(p<.05)가 보였으며, 사회적 능력 항목중 활동성과 사회성에서 유의한 차이(p<.0001)를 보였다. 지능저하등 다른질환군이 동반된 군에서는 사회기술 평가 척도의 사회기술 항목에서 유의한 증가(p<.05)를 보였다. 그러나 각 집단간의 치료효과에 대해서는 세 집단사이에서 유의한 차이는 보이지 않았다. 위 결과로 보아 주의력결핍 과잉운동장애 아동에서 사회기술훈련 치료프로그램은 통계적으로 유의미할 만한 사회기술 향상은 보이지 않았다. 반면 품행장애가 동반된 군에서 공격적 행동의 감소를 가져오고 인지능력이 떨어진 군에서 사회기술 호전을 보이는 등 아동의 특성 및 동반증상에 따라 사회기술의 호전 및 증상의 변화에 도움이 됨을 확인할 수 있었다. 문제이며, 이것을 예방하고 치료하기 위해서는 우리 의사들의 적극적 관심이 필요하다고 생각하였다.)에서 자기파괴적인 행동이 있었으며 46명(29.3%)에서 충동성이 동반되었고 35명(22.3%)에서 유뇨증이 관찰되었다. 환자의 발병연령과 내원시 연령, 병의 이병 기간, 강박증상의 정도 사이에 통계적으로 유의한 양성의 상관관계가 있었고 과잉행동성과 음성의 상관관계가 있었다. 과잉행동성과 충동성, 강박성, 야뇨증, 자기파괴적 행동사이에 통계적으로 유의한 양성의 상관관계가 있었다. 환자의 강박증상의 정도와 과잉행동성, 수면장애, 자기파괴적 행동 사이에 통계적으로 유의한 양성의 상관관계가 있었다. 본 연구 결과 저자들은 외래에 내원한 뚜렛장애 환자의 임상적 특성이나 동반된 행동상 문제들이 이전 연구와 크게 상이하지 않음을

  • PDF