• Microbiology and Biotechnology Letters
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• v.26 no.4
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• pp.275-282
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• 1998

To investigate the increased acid tolerance of a acid-resistant mutant Leuconostoc paramesenteroides P-100 improved as a kimchi starter, proton permeability, ATPase acitivity, glycolysis activity, $Mg^2$sup +/ releasement, and membrane fatty acid composition were studied and comprised with its wild type Leuconostoc paramesenteroides Pw. In the proton permeability experiment, the maximum values of the average half time (t$\_$1/2/) of pH equilibration through the cell membrane of the Pw and the P-100 were about 6.4 min and 7.8 min in 150 mM KCI solution, respectively. In the 3% NaCl solution, the t$\_$1/2/ values of the Pw and the P-100 were 5.5 min and 6.9 min, respectively. The values and pHs of maximal specific activities of ATPase originated from the Pw and the P-100 were 0.5 unit/mg protein and 0.78 unit/mg protein at pH 6.0, respectively. The result of pH dependence of glycolysis showed that the P-100 had higher activities than that of Pw except at pH 7.0. The releases of magnesium from the Pw and the P-100 were observed about 54.5% and 23.2% at pH 4.0 after 2 hours, respectively. The results of comparison of membrane fatty acid composition of the Pw with the P-100 showed that C$\_$8:0/, C$\_$9:0/, C$\_$10:0/, C$\_$11:0/, C$\_$18:0/, and C$\_$19:0,cyclo/ were major different fatty acids between two strains and the content of C$\_$18:1/, and C$\_$19:0,cyclo/ were 2.8%, N.D (not detected) in the Pw and 0.4%, 2.3% in the P-100. These results indicated that acid tolerance of the P-100 was significantly improved in comparison with its wild type Pw.

• Korean Journal of Environmental Agriculture
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• v.17 no.4
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• pp.352-357
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• 1998

This experiment was conducted to find out the effects of major nutrient levels(N, P, K) on ozone susceptibility of tomato plants(Lycopersicon esculentum Mill, cv. Pink Glory). Plants were grown in water culture system. A half-strength of Hoagland's nutrient solution was considered as a standard formulation($N_{100}$ $P_{100}$ $K_{100}$). The levels of major nutrients were adjusted through addition or removal of several fertilizer salts from the standard solution. Top growth was significantly decreased at the low nitrogen level or phosphorus removal condition. P- and K-contents of leaves were greatly decreased by removal of salts containing P and K from the nutrient solution. The rate of ozone injury was significantly increased when potassium was removed. However, the influence of nitrogen and phosphorus levels or high potassium level on injury occurrence did not show statistical significance compared to the standard solution. Ozone exposure resulted in reduction of chlorophyll, and increase of ethylene production, electrolyte leakage and malondialdehyde(MDA) contents. These changes were much more enhanced in plants grown at the potassium removal solution. Whereas the activity of superoxide dismutase(SOD) was low at the potassium removal treatment and this tendency remained after ozone exposure. These results indicated that potassium nutrient level in tomato plants is closely associated with the susceptibility to ozone injury.

• The Korean Journal of Food And Nutrition
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• v.31 no.5
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• pp.587-597
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• 2018

The quality characteristics of pudding products comprising of Korean Olbyossal (traditional steamed rice), added at different percentages, were examined to explore the application of Olbyossal to develop different types of processed food products. Five groups of pudding were produced containing 0%, 25%, 50%, 75%, and 100% of Olbyossal. Evaluation of their quality characteristics and antioxidant activities revealed that the control group (with no added Olbyossal) had the highest moisture content and pH (p<0.001). The sugar content and turbidity was highest in the 100% Olbyossal group and lowest in the control group (p<0.001). The salinity was lowest in the 100% Olbyossal group (p<0.001). In terms of color values, the L-value (p<0.001) and a-value were highest in the control group, while the b-value was highest in the 100% group (p<0.001). Texture properties revealed that hardness (p<0.001), springiness (p<0.01), and chewiness (p<0.001) decreased as the percentage of Olbyossal increased. Increase in adhesiveness was noted with an increase in the percentage of Olbyossal (p<0.01). Based on the sensory evaluation of Olbyossal pudding in terms of appearance (p<0.05), flavor (p<0.05), taste (p<0.01), texture (p<0.001), and overall quality (p<0.001), the 100% group showed the best results. Examination of Olbyossal pudding revealed that antioxidant activities, radical scavenging activities for DPPH and superoxide anion, and reducing power increased as the percentage of Olbyossal increased (p<0.001). Therefore, pudding containing 100% Olbyossal is considered as the ideal product with reference to the high level of preference and product quality.

• Food Engineering Progress
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• v.15 no.4
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• pp.338-345
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• 2011

The pH, acidity, protein, color, reducing sugar, total sugar, alcohol and organoleptic characteristics of Takju containing 100% short grain rice (100 S), 100% long grain rice (100 L), 50 S+50% puffed rice powder (50 P) and 50 L+50 P were examined. After a drastic decrease during the initial stage of fermentation, the pH gradually increased except 100 L. Protein peaked at day 1 and decreased. A higher protein was noticed in puffed samples than in rice samples (p < 0.05). The Hunter "L" value decreased after a rise during the beginning of fermentation. Puffed samples showed higher reducing sugar than rice at day 0. Total sugar was similar to reducing sugar. A drastic increase in alcohol concentration was detected at day 2. Higher alcohol was observed in short grain than long grain and also in puffed samples than in rice (p < 0.05). A significant difference between 100 S and 100 L was observed in a sensory evaluation (p < 0.01); while no preference was detected.

• Biomedical Science Letters
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• v.19 no.4
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• pp.344-347
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• 2013

The S100A8 and S100A9 proteins play important roles in inflammatory diseases. The house dust mite acts as a major allergen that induces allergic diseases. We investigated the effect of the house dust mite on S100A8 and S100A9 protein expression in monocytes. We also examined the effect of CCL2, a powerful monocyte chemoattractant, on the expression of both proteins. Extract of Dermatophagoides pteronissinus (DP), recombinant Der p 1 and Der p 2, or CCL2 had no effect on S100A8 and S100A9 expression in human monocytic THP-1 cells. Monocytes were isolated from healthy donors and treated with DP, Der p 1, and Der p 2. S100A8 expression in monocytes increased after a 24 h stimulation with DP, Der p 1, and Der p 2, and CCL2 also increased S100A8 production. However, S100A9 expression in monocytes was not altered by DP, Der p 1, Der p 2, or CCL2. These results indicate that house dust mite and CCL2 may trigger an inflammatory response by altering S100A8 expression.

• Korean Journal of Veterinary Research
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• v.30 no.2
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• pp.163-170
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• 1990

The blood chemical values and blood enzyme activities were examined from 74 healthy mixed breed dogs in the area of Seoul. The results obtained are summarized as follows; 1. Mean${\pm}$SD values and ranges of glucose were $61.97{\pm}8.41mg/100ml$ and 47.28~81.67mg/100ml, of blood urea nitrogen(BUN) $15.99{\pm}2.31mg/100ml$ and 8.21~21.31mg/100ml, of total protein(TP) $8.17{\pm}0.93g/100ml$ and 6.06~9.91g/100ml, of albumin $4.16{\pm}0.47g/100ml$ and 2.81~5.15g/100ml, of globulin, $4.01{\pm}0.64g/100ml$ and 2.72~5.54g/100ml, of albumin/globulin(A/G) ratio $1.06{\pm}0.17$ and 0.71~1.42, of cholesterol(Chol) $187.33{\pm}19.78mg/100ml$ and 128.70~222.90mg/100ml, of total bilirubin(TB) $0.73{\pm}0.14mg/100ml$ and 0.43~1.16mg/100ml, of phosphorus(Pi) $5.25{\pm}1.00mg/100ml$ and 2.61~7.72mg/100ml, of calcium(Ca) $10.76{\pm}1.08mg/100ml$ and 8.24~12.60mg/100ml, of triglyceride(TG) $89.48{\pm}21.16mg/100ml$ and 47.80~133.00mg/100ml, respectively. 2. The glucose value in the age group of 7~12 months was higher (p<0.01) but in the age group of 3~4 years was lower (p<0.05) than the total glucose value. The TP value in the age group of 7~12 months was lower (p<0.01) but in the age group of 1~2 years was higher (p<0.05) than the total TP value. The globulin value in the age group of 7~12 months was lower (p<0.01) but in the group of 1~2 years was higher (p<0.01) than the total globulin value. The A/G ratio value in the age group of 7~12 months was higher (p<0.05) but in the age group of 1~2 years was lower (p<0.05) than the total A/G ratio value. The phosphorus values in the age group of 1~2 years and the age group of 3~4 years were lower (p<0.01, p<0.001) than the total phosphorus value. The calcium value in the age group of less than 6 months was higher (p<0.05) but in the age group of 7~12 months was lower (p<0.001) than the total calcium value. 3. Mean${\pm}$S.D. values and ranges of alkaline phosphatase(AP) were $72.47{\pm}19.73IU/l$, and 28.13~105.00IU/l, of lactic dehydrogense(LDH) $159.46{\pm}45.11IU/l$ and 60.63~265.30IU/l, of serum aspartate aminotransferase(AST) $38.64{\pm}8.62IU/l$ and 21.47~70.58IU/l, of serum alanine aminotransferase(ALT) $34.88{\pm}11.30IU/l$ and 14.51~73.17IU/l, respectively. 4. The AP value in the age group of 7~12 months was higher (p<0.05) but in the age group of 1~2 years was lower (p<0.01, p<0.001) than the total AP value. The LDH value in the age group of less than 6 months was higher (p<0.001) but in the age group of 1~2 years and the age group of 3~4 years were lower (p<0.05) than the total LDH value. The serum AST value in the age group of 3~4 years was lower (p<0.01) than the total SGOT value. The serum ALT value in the age group of 7~12 months was higher(p<0.05) than the total SGPT value.

• Journal of Sericultural and Entomological Science
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• v.14 no.1
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• pp.9-13
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• 1972

On the seventh day of mounting male and feamale cocoons are distinguished. A part of those male and female pupae is irradiated for 40 min. with 1,000r-ray irradiation. Those eggs obtained from the irradiated pupae are divided into six groups of 5, 15, 45, 90, 720 and 2,400 minutes after laying. The cahanges of accid soluble phosphorus in the each group of eggs are as follows. 1. In the control group of the maximum quantity in acid soluble phosphorus was 1,456r/100mg D.P. in the 2,400 minutes group, while the minimum quantity was 1,233r/100mg D.P. in the 720 minutes group. 2. In the mated group of ＊irradiated female moths and control male moths, the quantity of acid soluble phoshorus was small as compared with the control group in the five minute group of 1,288r/100mg D.P. and in the 15 minutes group of 1,299r/100mg D.P. while the quantity increased to 1,484r/100mg D.P. in the 45 minutes group. The quantity began to decrease again to 1,400r/100mg D.P. in the 2,400 minutes group which was smaller than that of the control group. The quantity was slightly small in the silkworm eggs laid in the early stage when compared with that of mated group of ＊irradiated male moths and control female moths. Both groups showed the larger quantity in the 720 minutes group than that of the control group. The quantity of acid soluble phosphorus in the mated group of control female moths .and ＊irradiated male moths was larger in variation than in the mated group of control male moths and ＊irradiated female moths. 3. In the mated group of ＊irradiated male and female moths the largerst quantity was 1,760r/100ms D.P. in 2,400 minutes group and second was 1,736r/100mg D.P. in 90 minutes group, and third was 1,288r/100mg D. P., in 5 minutes group. Generally the quantity was small in silkworm eggs laid by the irradiated one in the early stage than the control group, but in the later period of egg the quantity began to increase more than that of control group.

• Journal of Environmental Science International
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• v.7 no.2
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• pp.141-148
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• 1998

Effects of alumlnlum(AA), soil pH and calcium(Cal on growth response and heavy metal accumulation and regulation of nitrogen fixation In Melilotus suaveolens seedlings, a biennial legume plant dominating in the disturbed area, were quantitatively analyzed during the growing periods. Accumulation of metals In each organ of M. suaveojens was Increased UC the lowering of pH. Al contents In leaf and root treat- ed with 30ppm Al at pH 4.2 on the 28th day after treatment were 8 and 11 folds higher than those of control, while the contents with 100ppm AA were 21 and 24 folds as compared to control. The significent inhibition in Al contents was induced by 100ppm AA and Ca at pH 6.5. Increased metals inhibited higher at pH 4.2 and the growth inhibition due to Al was reversed by the addition of Ca, suggesting that growth correlates to the pH value. Chlorophyll contents in leaves increased during growth stave were inhibited by Al treatments. The biomass was decreased UD the lowering of pH and the increase of concentrations. 100ppm Ca treatment resulted in 5.1-5.9% increase of the biomass as compared to that of 100ppm Al. Specific nitrogen fuation activities In nodules In the 100ppm Al at pH 4.2 and pH 6.5 were reduced to 35. 2% and 52.2% of control, on the 28th day after treatment, while the combination of Al and Ca induced reduced by 10% as compared to control with the treatment of 100ppm AA at pH 4.2 due to the inhibition bination of 100ppm Al and Ca and lowering of pH.

• Food Science and Preservation
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• v.19 no.6
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• pp.833-840
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• 2012

In this study, purple sweet potato muffins were made with the addition of 0, 25, 50, 75, and 100% rice flour, and their quality characteristics were determined. The weight and height increased with the increase in the amount of rice flour added. The moisture contents decreased significantly. As the amount of rice flour added increased, the pH and acidity decreased. The lightness and yellowness of the rice flour decreased significantly, but the redness increased with increasing purple sweet potato content. The textural properties, such as the cohesiveness, chewiness, and brittleness, were significantly lower in the groups with added rice flour. The DPPH radical scavenging activity was significantly higher in the purple sweet potato muffins to which 50 and 75% rice flour were added compared to those in the control. The results of the sensory evaluation showed that there was no significant difference in flavor among the samples. The chewiness was higher with 50, 75, and 100% rice flour than with 0 and 25% rice flour. The groups with 50 and 75% rice flour had significantly higher scores for overall acceptability compared to the control group.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1239-1244
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• 2003

Microcapsules were prepared by coacervation method using acetone/liquid paraffin system to control the ripening of kimchi. Eudragit E100, which was soluble at below pH 5.0 in aqueous solution, was used to make microcapsules to be sensitive to acidity of kimchi. The microcapsules with Eudragit E100 containing grapefruit seed extract (GFSE) showed the highest yield of 92.13%, the size of microcapsules was decreasing as increasing the amount of aluminium stearate, a dispersing agent. Morphology of the microcapsules determined by scanning electron microscopy showed spherical forms. GFSE, encapsulated antimicrobial agents, was quickly released at acidic buffer (pH 4,5,6) within 1 storage day. However, 70% of encapsulated GFSE in Eudragit E100 microcapsules was continuously released at pH 7 till 3 days, and it was sustained till 9 days. Characteristics of kimchi containing microcapsules of GFSE were analysed with ripening period. Decease of pH in kimchi was retarded with the added GFSE microcapsules till 2 days of fermentation, but GFSE did not affect pH in kimchi after 3 days. Total numbers of microorganisms and lactic acid microorganisms in kimchi were decreased with increasing the amount of the added GFSE microcapsules, however, the effect of controlled released GFSE from pH sensitive Eudragit E100 microcapsules was hard to detect. These results suggest the possibility of pH sensitive microcapsules for high qualify of kimchi.