Study on the Change of Acid Soluble Phosphorus and Influence of Irradiation on Embryonic Developmental Silkworm Egg, Bombyx mori L. (Part. II)

가잠란발생과정에서 Acid Soluble Phosphorus의 변동과 방사선 영향에 관한 연구 (제2보)

On the seventh day of mounting male and feamale cocoons are distinguished. A part of those male and female pupae is irradiated for 40 min. with 1,000r-ray irradiation. Those eggs obtained from the irradiated pupae are divided into six groups of 5, 15, 45, 90, 720 and 2,400 minutes after laying. The cahanges of accid soluble phosphorus in the each group of eggs are as follows. 1. In the control group of the maximum quantity in acid soluble phosphorus was 1,456r/100mg D.P. in the 2,400 minutes group, while the minimum quantity was 1,233r/100mg D.P. in the 720 minutes group. 2. In the mated group of ＊irradiated female moths and control male moths, the quantity of acid soluble phoshorus was small as compared with the control group in the five minute group of 1,288r/100mg D.P. and in the 15 minutes group of 1,299r/100mg D.P. while the quantity increased to 1,484r/100mg D.P. in the 45 minutes group. The quantity began to decrease again to 1,400r/100mg D.P. in the 2,400 minutes group which was smaller than that of the control group. The quantity was slightly small in the silkworm eggs laid in the early stage when compared with that of mated group of ＊irradiated male moths and control female moths. Both groups showed the larger quantity in the 720 minutes group than that of the control group. The quantity of acid soluble phosphorus in the mated group of control female moths .and ＊irradiated male moths was larger in variation than in the mated group of control male moths and ＊irradiated female moths. 3. In the mated group of ＊irradiated male and female moths the largerst quantity was 1,760r/100ms D.P. in 2,400 minutes group and second was 1,736r/100mg D.P. in 90 minutes group, and third was 1,288r/100mg D. P., in 5 minutes group. Generally the quantity was small in silkworm eggs laid by the irradiated one in the early stage than the control group, but in the later period of egg the quantity began to increase more than that of control group.

상족후 7일째 수견하고 자웅을 감별하여 일부는 방사선(${\gamma}$-선) 1,000${\gamma}$에서 40분간 조사시켜 화아 교미후 산란후 시간을 5분, 15분, 45분, 90분, 720분, 2,400분의 6구로 나누어 란내 acid soluble phosphorus의 변동을 분석 검토한 결과 다음과 같은 결과를 얻었다. 1. 표준구(대조구)에서는 산란후 90분에 1,288${\gamma}$/100 mg D.P.로 점차 감소하여 720분에 1,232${\gamma}$/100mg D.P.로 가장 적은량을 나타냈으나 2,400분에 1,456${\gamma}$/100mg D.P.로 가장 많은 량을 나타냄을 알 수 있었다. 2. 방사선처리한 ♀에 표준구(무처리) ♂놈을 교미시킨 구에서는 표준구(대조구)에 비하여 산란초기 5분에 1,288${\gamma}$/100mg D.P. 15분에 1,299${\gamma}$/100mg D.P.로 다소 적은량을 나타냈으나 45분에 1,484${\gamma}$/100mg D.P.로 증가된 량을 보이다가 2,400분에 1,400${\gamma}$/100mg D.P.로 표준구(대조구)보다 적은량을 나타냈다. 그러나 표준구에 ♀놈에 방사선처리구에 ♂놈을 교미시킨 구와 비교할 때 초기에는 다소 적은 량이었으나 큰 차리는 확인할 수 없었으며 양구 다같이 산란후 15분에서 720분까지 양적으로 표준구(대조구) 많았음을 인정할 수 있고, 방사선처리한 ♀놈에 표준구(무처리)의 ♂놈을 교미시킨 구보다 표준구의 ♀놈에 방사선처리한 ♂놈을 교미시킨 구에서 변동이 크고, lipid phosphorus(Part I)의 경우와 상반된 변동이 이루어지는것을 알 수 있었다. 3. ♀.♂ 다같이 방사선을 처리하여 교미시킨 구에서 산란후 90분에 1,736${\gamma}$/100mg D.P, 2,400분에 1,760 ${\gamma}$/100mg D.P.로 많은 량을 나타냈고 5분에 1,288${\gamma}$/100mg D.P.로 가장 적은량을 나타냈으며 전반적으로 보아 산란초기에는 표준구보다 적었으나 그 후에는 표준구보다 크게 증가하고 변동이 심한것을 확인할 수 있었다.