• Microbiology and Biotechnology Letters
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• v.16 no.4
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• pp.282-286
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• 1988

Growth kinetic parameters for mass cultivation of Chinese Hamster Ovary (CHO) cells are estimated by measuring oxygen uptake rates. It Is found that there is strong correlation between cell growth and oxygen consumption, showing that correlation factor is 0.83. Derived linear model predicts actual cell density very well. It tells that oxygen uptake rate can play important role in indirectly measuring cell density when conventional method of estimating cell density is no longer meaningful due to heavy cell clumpings. Cell yield per oxygen consumption, $Y_{\chi}o$ and mass transfer coefficient for oxygen, Ka are also estimated as 1.26$\times$10$^4$cells/mmole $O_2$ consumed and 1.01/h, respectively. Average specific growth rate over all runs is 2.891/day for CHO cells with producting 2 grams of tPA per day under continuous perfusion operations.

• The Korean Journal of Physiology
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• v.24 no.2
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• pp.249-260
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• 1990

Maximal cardiac output and oxygen uptake $(VO_{2max})$ were measured during treadmill exercise for seven top-class marathoners and nine non-athletes using impedance cardiograph developed by one of the authors (DW Kim). Results of this study are summarized as belows. 1) New shoes with sponge and silicon rubber attached to the soles were developed to reduce motion artifact during treadmill exercise. Ensemble everaging techneque with the developed shoes was also used to improve the measurement of stroke volume using impedance cardiography. 2) Maximal cardiac output of the athletes, 14.98 L/min, was significantly higher than that of the non-athletes, 13.46 L/min. As maximal heart rate of the marathoners is lower than that of non-athletes, stroke volume of the former is significantly larger than that of the latter. 3) $VO_{2max}$ of the marathoners, 59.38 ml/kg/min, was higher than that of the non-athletes, 40.22 ml/kg/min. At the anaerobic threshold. $VO_{2max}$ of the former was 62.3% of $VO_{2max}$ and this was significantly higher than that of the non-athletes, 57.2%, This results indicates that the marathoners have higher aerobic capacity than the non-athletes. 4) The marathoners showed larger $VO_2$ than the non-athletes at the same cardiac output, indicating that a-v $O_2$ of the former is higher than that of the latter. 5) Maximal systolic pressure of the marathoners was higher than that of the non-athletes, and so was maximal rate-pressure products. These results indicate that heart oxygen consumption rate $(hVO_2)$ of the marathoner is higher than that of the non-athletes is mainly due to higher stroke volume. And higher oxygen consumption of the marathoners is due to higher stroke volume. And higher oxygen consumption of the marathoners is due to their larger a-v $O_2$. The marathoners show both higher threshold and $VO_{2max}$. Especially, measurement of cardiac output during treadmill exercise by improved impedance cardiography is expected to contribute in study of cardiac function of athletes.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2002.05a
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• pp.3-9
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• 2002

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1996.06a
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• pp.147-148
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• 1996

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.83.1-83.1
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• 2007

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• Journal of the Ergonomics Society of Korea
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• v.25 no.2
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• pp.11-22
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• 2006

The muscular-skeletal disorders(MSDs) that have become a major issue recently in Korean industrial safety area are mainly caused by manual material handling task. The objective of this study is to provide scientific data for the establishment of work safety standard for Korean workers through the experiments of lifting task under various conditions, in order to prevent the muscular-skeletal disorders in the industrial work site. Eight male college students were recruited as participants. Three different lifting frequencies(1, 3, 5 lifts/min) and three twisting angles(including the sagittal plane and two asymmetric angles; i.e., 0°, 45°, 90°) for symmetric and asymmetric tasks, respectively, with three lifting range from floor to knuckle height, knuckle to shoulder, floor to shoulder height for one hour's work shift using free style lifting technique were studied. The maximum acceptable weight of load(MAWL) was determined under the different task conditions, and the oxygen consumption, heart rate, and RPE were measured or recorded while subjects were lifting their MAWLs. The results showed that: (1) The MAWLs were significantly decreased as the task frequency and task angle increased.; (2) The heart rate, oxygen consumption, RPE significantly increased with an increase in lifting frequency although maximum acceptable weight of lift decreased.; (3) The highest heart rate and oxygen consumption was recorded at the lifting range of floor to shoulder, followed by floor to knuckle and knuckle to shoulder.; (4) The RPE value showed that subjects perceived more exertion at the high frequency rate of lifting task and lifting range of floor to shoulder height. (5) The modeling for MAWL using isometric strength, task angle and lifting frequency were developed. It is expected that use of the results provided in this study may prove helpful in reducing MMH hazards, especially from lifting tasks for Korean, and can be used as a basis for pre-employment screening.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.2
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• pp.100-105
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• 2005

Two species of fish and five species of marine invertebrate showed different tolerances to the toxicity of composite slag and bituminaus coal. Especially, Hemicentrotus pulcherrimus and young Haliotis discus hannai displayed marked differences in tolerance from. H. pulcherrimus and young H. discus hannai showed lethal effects at higher concentrations than those concentrations of the composite slag in the 1.0 and $0.4\%$ range, respectively. H. pulcherrimus showed no lethal effects at a lower concentration of $1.0\%$ composite slag and some differences in the rate of oxygen consumption with this concentration of composite slag. The lethal effects of bituminous coal on marine and fisheries organisms, even with higher concentrations, were not observed. At a higher concentration than that of 500 mg/L (ppm) of bituminous coal, decrease effects appeared in the rate of oxygen consumption of the experimental organisms. Taking into consideration that the experimental concentration of composite slag and bituminous coal were impracticable in the ocean, the results of this experiment suggest that composite slag and bituminous coal pose no real threat to marine or fisheries organisms.

• Fisheries and Aquatic Sciences
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• v.5 no.3
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• pp.191-199
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• 2002

Pacific oysters Crassostrea gigas (Thunberg) were collected on April, 1999 and March­September, 2000 from Goseung Bay along the southern coast of Korea. The oysters tested cp;;ected from a depth of 0.5-2 m in which they cultured by a long line hanging method. The oxygen consumption rates (OCR) of oysters held under constant temperature and darkness (CC), were determined using an automatic intermittent-flow-respirometer (AIFR). Depending on holding periods after oyster collection, the experiments were divided into two groups: Group 7-d (held to ambient temperature for ca. 7 days) and Group 2l-d (held to ambient temperature for ca. 21 days). The OCR for Group 7-d single oyster displayed two peaks every day under CC, while Group 2l-d single oyster showed one peak every day. It is likely that the rhythmic patterns 02.6-12.8 hours) of the OCR in the Group 7-d single oyster may have been influenced by tidal currents at the sampling site. The rhythmic patterns (24.3-24.7 hours) in the Group 2l-d single oyster may have been shifted from two peaks to one peak each day under CC. The present study concludes that the OCR rhythm of wild oysters in nature is governed by two lunar-day clocks (24.8 hours); one driving one peak and the other driving the second peak. When oysters are subjected to the long-term CC conditions, one of the two-clock systems is depressed or only intermittently becomes active. Jpwever. the OCR rhythms by two to three oysters occurred arrhythmic patterns during the experiments and exhibited some evidence of weak rhythmicity of compared to those of a single oyster. It could be partly due to differences group effects.

• Korean journal of applied entomology
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• v.29 no.4
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• pp.286-293
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• 1990

Studies were carried out to investigate the change in protiens and carbohydrates from diapausing pupae of the Oriental tobacco budworm, Heliothis assulta Guenee. The oxygen consumption rate of non-diapausing pupae through the whole pupal period showed an U-shaped curve, while that of diapausing pupae decreased to a mean level of 20$\mu$1/g/hr. But the rate of oxygen consumption increased in diapausing pupae before their emergence. The body weight of diapausing pupae showed almost no change during 12 days after pupation. The total contents of major carbohydrates and soluble proteins were higher in diapausing pupae than those in non-diapausing pupae and the change in carbohydrate and protein contents was more dynamic in non-diapausing pupae than that of diapausing pupae. the electrophoretic band patterns of proteins were similar in both of diapausing and non-diapausing pupae. Diapausing pupae increased their haemolmph osmolarity further when they were exposed to low temperatures.

• Ocean and Polar Research
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• v.27 no.1
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• pp.1-13
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• 2005

The samples for organic carbon analysis were collected between $5^{\circ}\;and\;17^{\circ}N$ along $131.5^{\circ}W$ in the northeast Pacific KODOS (Korea Deep Ocean Study) area. The mean concentration of total organic carbon (TOC) in the surface mixed layer $({\sim}50 m)$ was $100.13{\pm}2.05{\mu}M-C$, while the mean concentration of TOC in the lower 500m of the water column was $50.19{\pm}4.23{\mu}M-C$. A strong linear regression between TOC and temperature $(r^2=0.70)$ showed that TOC distribution was controlled by physical process. Results from the linear regression between chlorophyll-a and TOC, and between chlorophyll-a and particulate organic carbon (POC), decreasing of dissolved organic carbon (DOC) in the surface layer caused by non-biological photo-oxidation process. Below the surface layer, biological production and consumption occurred. DOC accumulation dominated in the depth range of $30{\sim}50m$ and DOC consumption occurred in the depth range of $50{\sim}200m$. TOC was inversely correlated with apparent oxygen utilization (AOU) and TOC/AOU molar ratios ranged from -0.077 to -0.21. These ratios indicated that TOC oxidation was responsible fur $10.9{\sim}30.1%$ (mean 20.2%) of oxygen consumption in the NE Pacific KODOS area. In the euphotic zone, distributions of dissolved and particulate organic matter were controlled by photo-chemical, chemical, biological and physical processes.