• Title/Summary/Keyword: oviposition

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Relationship between Intersequence Pauses, Laying Persistency and Concentration of Prolactin during the Productive Period in White Leghorn Hens

  • Reddy, I.J.;David, C.G.;Singh, Khub
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.686-691
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    • 2005
  • Prolactin is considered to influence the taking of pauses in between ovulatory sequences in White Leghorn hens. Therefore modulating concentrations of prolactin using bromocriptine - a dopamine agonist during early life (17 to 36 weeks of age) could overcome the inhibitory effects of high concentration of prolactin on ovarian activity. The effect of modulation of prolactin concentration on egg production, sequence length and inter sequence pauses were studied by analyzing the oviposition records from 19 to 72 weeks were studied and compared with untreated controls. Bromocriptine administered subcutaneously (100 $\mu$g kg$^{-1}$ body weight or orally through feed (640 $\mu$g day$^{-1}$ bird$^{-1}$) resulted in a steady and sustained decrease in prolactin levels (p<0.01) during and after the withdrawal of treatment up to one reproductive cycle (72 weeks of age). The treated birds had comparatively longer sequences (p<0.01) and fewer pauses (p<0.01). Egg production increased (p<0.01) by fourteen per cent through subcutaneous administration and eleven per cent through oral feeding, over the control birds. It is concluded that the physiological pauses that occur during ovulatory sequences can be disrupted effectively using bromocriptine. Prolactin levels are modulated which may interfere with the follicular recruitment and subsequent oviposition thereby improve egg laying potential of the bird.

Gene functional analysis of Harmonia axyridis by in vitro transcription

  • Park, Sang-Eun;Youn, Young-Nam
    • Korean Journal of Agricultural Science
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    • v.46 no.3
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    • pp.471-488
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    • 2019
  • Random genes were screened in two transforming ways to investigate the new genes of a ladybug using the Harmonia axyridis cDNA library stock cell cloned in the LITMUS 28i vector in a previous study. Phenotypic variation was observed after injection of the synthesized double-stranded RNA through the in vitro transcription process. The cDNA library of H. axyridis was transformed into E. coli $DH5{\alpha}$ and 10B competent cells by heat shock. Analysis of the nucleotide sequences of the 42 clones with the insert DNAs revealed that 21 clones were homologous with the genes of insects, and only one clone had a gene from H. axyridis. Thirteen of the 21 insect genes were homologous with genes from coleopteran insects. Fourteen genes were selected, which were identified by the gene screening results, and were synthesized as double-stranded RNA through in vitro transcription. One microgram of the synthesized double-stranded RNA between segments T1 and T2 were injected using a syringe into each anesthetized fourth larvae which were under 2 days old. As a result, a phenotypic variation appeared in the larva injected with the two genes. While the eggs of H. axyridis injected with distilled water hatched out three days after oviposition, the eggs of H. axyridis injected with dsHma 06 did not hatch but become shrivel a week after oviposition. Most of the H. axyridis injected with dsHma 08 died and were unable to complete the pupation or eclosion during ecdysis.

Studies on bionomical characteristics of Papilio xuthus (호랑나비(Papilio xuthus)의 생육특성에 관한 연구)

  • Lee, Sang-Hyun;Kim, Se-Gwon;Kim, Nam-Ee;Bae, Kyoung-Sin;Choi, Young-Cheol
    • Journal of Sericultural and Entomological Science
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    • v.51 no.2
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    • pp.173-179
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    • 2013
  • This study was investigation for primary bionomical characteristics, emergence rate of wintering type pupa and oviposition preference, of Papilio xuthus. The emergence rate of wintering type pupa stored at cold chamber($4{\pm}1^{\circ}C$) was 85%, after 10 months. The average emergence rate of wintering type pupa during storage was 89.6%, and the emergence periods were $7.9{\pm}1.9days$ in room temperature. Mating ratio in large oviposition room, $6,000{\times}6,000{\times}3,500$ mm($W{\times}L{\times}H$), was $86.7{\pm}5.8%$ higher than $63.3{\pm}15.3%$ in small room, $2,500{\times}3,000{\times}2,000$ mm($W{\times}L{\times}H$). But the number of eggs in the large room were $137.0{\pm}16.5$ fewer than $141.7{\pm}20.4$ in the small room. As a result of oviposition preference experiment on three different host plants, 10 mated female laid $141.7{\pm}27.8$ on the leaves of Phellodendron amurensew more than $67.7{\pm}20.6$ on Zanthoxylum schinifolium and $77.0{\pm}21.8$ on Citrus unshiu. The egg periods were $4.4{\pm}0.8$ days after oviposition, and the hatchability was 92.2% in $25^{\circ}C$ room condition. The larval period were $19.9{\pm}2.1$ days in the high temperature, long day condition($25^{\circ}C$, 14L : 10D). The headwidth of each developmental larval stage were $0.72{\pm}0.02$(1st), $1.19{\pm}0.02$(2nd), $1.65{\pm}0.05$(3rd), $2.43{\pm}0.07$(4th), $3.21{\pm}0.12$(5th). The pupal ratio was 91.6%. The pupal period were $8.8{\pm}0.9$ days, and the emergence rate was 92.2%.

Host Dependent Oviposition and Development of Azuki Bean Weevil (Callosobruchus chinensis L.) in Different Leguminous Seeds (기주에 따른 팥바구미(Callosobruchus chinensis L.)의 산란 선호성 및 성장)

  • Kim, Seul Gi;Lee, Su Mi;Park, Jun Hong;Song, Kuk;Shin, Byung Sik
    • Korean Journal of Environmental Biology
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    • v.34 no.4
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    • pp.346-352
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    • 2016
  • To investigate the oviposition preference and development of azuki bean weevil (Callosobruchus chinensis L.), the following six different leguminous seeds were used in this study: red bean (Vigna angularis (Willd.) Ohwi & Ohashi), black soybean (Glycine max (L.) Merr.), soybean (Glycine max (L.) Merr.), seoritae (Glycine max (L.) Merr.), small black bean (Rhynchosia nulubilis) and kidney bean (Phaseolus vulgaris var. humilis Alef.). In the study of oviposition preference, the numbers of eggs per leguminous seed on red bean, black soybean, soybean, seoritae and small black bean were 1.23, 0.61, 0.69, 1.05 and 1.13, respectively. The maximum daily number of eggs was observed at 48 hours and the minimum was at 96 hours. According to each host leguminous seed, developmental time for each host seed was different. The shortest adult emergence time was on red bean (25.27 days). The other five leguminous seeds increased or doubled the adult emergence time. Adult emergence rates feeding on red bean, seoritae, black soybean, soybean, small black bean were 83.33%, 28.23%, 27.87%, 20.44%, and 11.59%, respectively. Emergence rate on red bean was four times higher than the rate on other seeds. The longevity of emerged female adults was almost all longer than that of males. The male adults weighed the lowest of feeding on small black bean. Female adults weighed the lowest of feeding on soybean. Adult weights were the heaviest for both males and females feeding on red bean. As a result, hosts of azuki bean weevil could decrease oviposition rate, emergence rate, adult longevity, and adult weight but increase emergence time. Especially in kidney bean, adult was not completely emerged. No eggs were laid. These results suggest that there might be emergence inhibitors in kidney bean. These imformation might be used to control damages caused by azuki bean weevils.

Using Viable Eggs to Determine Oviposition Models and Life Table Analysis of Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) (톱다리개미허리노린재의 수정란을 이용한 산란모형과 생명표분석)

  • Ahn, Jeong Joon;Choi, Kyoung San;Koh, Sang Wook
    • Korean journal of applied entomology
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    • v.58 no.2
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    • pp.111-120
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    • 2019
  • Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) is an economically important insect pest of soybean and fruit trees. We investigated the temperature effects on the adult fecundity and longevity, and determined the parameters of oviposition models and life table at different constant temperatures 15.8, 19.7, 24.0, 27.8, 32.6, 34.0, and $35.5^{\circ}C$. R. pedestris females reproduced successfully from 19.7 to $35.5^{\circ}C$ except $15.8^{\circ}C$. The longevity of R. pedestris was longest at $15.8^{\circ}C$ and it decreased with increasing temperature (76.6 days at $19.7^{\circ}C$ and 20.6 days at $35.5^{\circ}C$). The number of total eggs and viable eggs was highest at $24.0^{\circ}C$ (193.5 and 151.2). Egg hatchability was highest at $27.8^{\circ}C$ (84.0%). We compared the results of oviposition models and life table parameters using both total eggs and viable eggs. The parameter value (c: the maximum reproductive capacity) (190 eggs) of temperature dependent total fecundity model using total eggs was higher than that of the model using viable eggs. When we analyzed the life table parameter the values of net reproductive rate and mean generation time using viable eggs were lower than those using total eggs. The oviposition models and life table analysis using viable eggs will be helpful to understand the real population transition of R. pedestris in agricultural system.

In Silico Analysis and Molecular Docking Comparison of Mosquito Oviposition Pheromone and Beta-asarone on the Mosquito Odorant Binding Protein-1 (In Silico 분자결합 분석방법을 활용한 MOP와 베타아사론의 열대집모기 후각단백질 활성 부위에 대한 결합 친화도 비교 분석)

  • Kim, Dong-Chan
    • Journal of Life Science
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    • v.28 no.2
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    • pp.195-200
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    • 2018
  • Beta-asarone is the well-known active ingredient of Rhizoma acori graminei. In this study, we investigated and compared the binding affinity of mosquito oviposition pheromone (MOP; (5R,6S)-6-acetoxy-5-hexadecanolide) and beta-asarone on the A domain of the mosquito odorant binding protein 1 (CquiOBP1) by in silico computational docking studies. The three-dimensional crystallographic structure of CquiOBP1 was obtained from the PDB database (PDB ID: 3OGN). In silico computational auto-docking analysis was performed using PyRx, Autodock Vina, Discovery Studio Version 4.5, and the NX-QuickPharm option based on scoring functions. The beta-asarone showed optimum binding affinity (docking energy) with CquiOBP1 as -6.40 kcal/mol as compared to the MOP (-6.00 kcal/mol). Among the interacting amino acids (LEU76, LEU80, ALA88, MET89, HIS111, TRP114, and TYR122), tryptophan 114 in the CquiOBP1 active site significantly interacted with both MOP and beta-asarone. Amino acids substitution (mutation) from non-polar groups to the polar (or charged) groups of the CquiOBP1 dramatically changed the X, Y, Z grid position and binding affinity of both ligands. These results significantly indicated that beta-asarone could be a more potent ligand to the CquiOBP1 than MOP. Therefore, the extract of Rhizoma acori graminei or beta-asarone can be applied to the fields of insecticidal and repellant biomaterial development.

Egg-forming and Preservation Methods of the Emma Field Cricket Eggs, Teleogrylius emma (Orthoptera: Gryllidae) (왕귀뚜라미(Teleogryiius emma)알의 실내 인공 채란 및 저장)

  • Kim Namjung;Hong Seong-Jin;Seol Kwang-Youl;Kwon Ohseok;Kim Seong-Hyun
    • Korean journal of applied entomology
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    • v.44 no.1 s.138
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    • pp.61-65
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    • 2005
  • Egg-forming and preservation methods for the indoor-rearing system of the emma field cricket, Teleogryllus emma, were investigated. The oviposition preference experiment on different mats, soil(natural oviposition mat) and $oasis^{\circledR}$(artificial oviposition mat), showed that T. emma preferred to oviposit on the $oasis^{\circledR}$. On the other hand, $oasis^{\circledR}$ had more inactive number of eggs laid per female and hatchability than another. However, the number of eggs laid per female and hatchability within 50 days after emergence were better than soil. The experiment on the hatching of the eggs showed that eggs could be stocked at $10^{\circ}C$ for 40 days with 14 day pre-period after laying, representing $62.1\%$ hatchability, when considering generation shortening. On the other hand, When it had been kept in cold storage for 90 days at $7.5^{\circ}C$ with 14 day pre-period after laying it, appeared to be the most suitable for long period-storage method by hatchability $65.1\%$.

Characteristic of Oviposition and Effect of Density Suppression by Yellow-colored Sticky Trap on Ricania shantungensis (Hemiptera: Ricaniidae) in Blueberry (블루베리에서 갈색날개매미충의 산란특성 및 황색끈끈이트랩의 산란 억제효과)

  • Kim, Dong Hwan;Kim, Hyeong Hwan;Yang, Chang Yeol;Kang, Taek Jun;Yoon, Jung Beom;Seo, Mi Hye
    • The Korean Journal of Pesticide Science
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    • v.20 no.4
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    • pp.281-285
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    • 2016
  • This study was conducted to investigate the characteristics of oviposition and the effect of density suppression by yellow-colored sticky trap on Ricania shantungensis in blueberry. The occurrence of an egg mass of R. shantungensis in the upper, middle and lower region were 56.6~60.2%, 23.8~28.1% and 11.7~19.7%, respectively. The number of egg masses in the branch was investigated. Percentage of the branch with one egg mass was greatest (50.9%) than with two (20.5%), three (14.6%) and over four (14.0%). The effect of yellow-colored sticky trap to reduce the number of R. shantungensis egg masses in blueberry was also investigated. In a month after yellow sticky trap installation, 17.1 adults of R. shantungensis were attracted per trap. Moreover, the number of egg masses on a tree in yellow-colored sticky trap plot was much lower (0.4) than control (1.3). Consequently, this result shows that use of yellow-colored sticky trap may contribute to decrease ovipisotion rate of R. shantungensis in blueberry.

Effects of rearing density on food consumption, adult mortality and mean number of hatchlings of Gryllus bimaculatus (Orthoptera: Gryllidae) (쌍별귀뚜라미, Gryllus bimaculatus (Orthoptera: Gryllidae)의 사육밀도가 섭식량, 성충사망률 및 부화 약충수에 미치는 영향)

  • Park, Young-Kyu;Lee, Hyung-Gun;Choi, Young-Cheol
    • Journal of Sericultural and Entomological Science
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    • v.51 no.2
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    • pp.89-94
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    • 2013
  • The aim of the present study is to investigate food consumption, hatching periods, adult mortality, the mean number of hatchlings and ovipositional trend of Gryllus bimaculatus De Geer. The rearing and experiments were conducted in the rearing room ($28{\pm}2^{\circ}C$, $60{\pm}10%$RH). As the adult rearing density increased from 50 to 400, it did not affect the average daily food consumption of adult crickets which was $0.067{\pm}0.008$ g a day. However, the daily radish consumption decreased from 0.128 to 0.068 g per day. 95.8% of eggs hatched by the sixth day after the beginning of hatching. The average mortality of the adults gradually increased, and reached 93.9% by the 8th week. Total number of hatchlings per female was $172.2{\pm}10.6$ ($mean{\pm}SD$) in 200 females rearing cages, which was noticeably less than $248.8{\pm}17.8$ ($mean{\pm}SD$) in 100 females rearing cages. Sex ratio of the crickets given in the rearing cages did not affect both the number of hatchlings and ovipositon rate. Oviposition rate periodically fluctuated as progressed, and it reached 81.5% of the total oviposition by the $3^{rd}$ week since the beginning of ovipoisiton.

Oviposition by the Adults of a Green Lacewing, Chrysopa formosa Brauer (Neuroptera: Chrysopidae), Reared on Artificial Diets (인공식이물(人工食餌物)에 의한 갑오풀잠자리 성충(成蟲)의 산란(産卵))

  • Park, Seung-Chang;Woo, Kun-Suk
    • Korean journal of applied entomology
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    • v.24 no.1 s.62
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    • pp.34-38
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    • 1985
  • Effects of some artificial diets containing pulverized silkworm pupae On the oviposition of a green lacewing, Chrysopa formosa, were studied. The pulverized silkworm pupae with honey seemed to be a good diet for the egg-laying activity of field-collected adults in the beginning of rearing, but their fecundity progressively decreased. Newly emerged adults. did. not lay eggs when fed the above diet, but they were able to lay eggs when the silkworm pupae were defatted and provided with honey. An adult female laid an average of 166.8, 110.2, 96.1, and 66.1 eggs after spending 5, 9, 9, and 11 days for the initiation of oviposition when supplied with various diets i.e. the cabbage aphid, defatted silkworm pupae with additive constituents, mixture of intact and defatted silkworm pupae with additive constituents, and defatted silkworm pupae with honey, respectively.

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