• 한국응용곤충학회지
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• 제54권3호
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• pp.199-209
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• 2015

가루깍지벌레는 열대지역에서 일반적인 해충으로 관상식물과 과수류의 큰 문제를 일으키는 해충이다. 캄보디아와 라오스에는 현재 가루깍지벌레과 22종 및 12종이 각각 보고되어 있다. 최근 이들 지역에서 가루깍지벌레과 연구를 수행하여 13종이 채집되었으며, 그 중 캄보디아에서 3종인 Dysmicoccus neobrevipes (Beardsley, 1959), Phenacoccus solani (Ferris, 1918), Rastrococcus iceryoides (Green, 1908) 와 라오스에서 6종인 Dysmicoccus neobrevipes (Beardsley, 1959), Paracoccus invectus (Williams, 2004), Phenacoccus solenopsis (Tinsley, 1898), Planococcus litchi (Cox, 1989), Planococcus minor (Maskell, 1897), Pseudococcus jackbeardsleyi (Gimpel & Miller, 1996)가 이들 국가로부터 처음 보고된다.

• Journal of Plant Biotechnology
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• 제38권3호
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• pp.234-240
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• 2011

The genus Paeonia belongs to the family Paeoniaceae having significant medicinal and ornamental importance. The present investigation was undertaken with an aim to understand phylogenetic relationships of three Paeonia species (P. lactiflora, P. obovata, and P. suffruticosa) that are widely distributed in China, Korea, and Japan, using nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequence and to compare the phylogeny results with investigations reported earlier using existed sequences of the same species. The size variation obtained among sequenced nrDNA ITS region was narrow and ranged from 722 to 726 bp. The highest interspecific genetic distance (GD) was found between P. lactiflora and P. suffruticosa or P. obovata. The phylogram obtained using our nrDNA ITS sequences showed non-congruence with previous hypothesis of the phylogeny between section Paeonia and section Moutan of genus Paeonia. This result was supported by the phylogenetic relations showed in the phylogram constructed with existed sequences in NCBI. The present study suggested that P. obovata belonging to section Paeonia was phylogenetically closer to P. suffruticosa representing section Moutan of genus Paeonia than P. lactiflora belonging to section Paeonia. The main reason of the paraphyly of section Paeonia is thought to be nucleotide additivity directly caused by origin hybridization. This study provides more sequence sources of genus Paeonia, and will help for further studies in intraspecies population, and their phylogentic analysis and molecular evolution.

• 화훼연구
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• 제16권4호
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• pp.275-283
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• 2008

태국에서 도입한 Curcuma 18종 및 품종의 생육 및 개화특성을 조사하여 용도별로 우수 품종을 선발하였다. Curcuma alismatifolia 'Chiangmai Pink', 'Tropic Snow', 'Chiangmai Dark Pink', 'Lady Di', 'Siam Violet Lily' 등은 화경장이 약 40~55cm 정도이고 절화수명도 매우 길어 절화용으로 적합하였으며, C. alismatifolia 'Cambodian Red', 'Cambodian Scarlet'와 C. sparganifolia 'Siam Ruby' 및 C. thorelii 'Chiangmai Snow'는 10-20cm 정도로 화경장이 짧아 분화용으로 적합하였다. 꽃(화서)의 수명은 대부분 30일 이상 유지되었다.

• 생약학회지
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• 제52권4호
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• pp.212-218
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• 2021

Fatsia japonica is grown wild to Eastern Asia, including Korea, Japan, and Taiwan and it is known as ornamental plant, and it is also known that pharmacological action. In this study, we have selected the stem of F. japonica with consideration about biological activities and amount of yield. In addition, four compounds (1-4) were isolated from the stem of F. japonica. Extensive spectroscopic and chemical studies established the structures of these compounds as maltose (1), begoniifolide A (2), leiyemudanoside B (3), leonticin F (4). All of the compounds were investigated for their anti-inflammatory, anti-neuroinflammatory, and neuro-protective effects on RAW264.7, BV2, and HT22 cells. However, among four compounds, there were no effects by maltose (1), begoniifolide A (2), leiyemudanoside B (3), leonticin F (4) on the anti-inflammatory, anti-neuroinflammatory, and neuro-protective action. This is the first report on the isolation of maltose (1), begoniifolide A (2), leiyemudanoside B (3), leonticin F (4) from the stem of F. japonica. Begoniifolide A (2), leiyemudanoside B (3), leonticin F (4) were isolated for the first time from this plant. It might be necessary to continue the further studies to find the biological active compounds isolated from the stem of F. japonica.

• The Plant Pathology Journal
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• 제40권1호
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• pp.40-47
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• 2024

Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.

• The Plant Pathology Journal
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• 제40권1호
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• pp.73-82
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• 2024

Gardenia (Gardenia jasminoides) is a popular and economically vital plant known for its ornamental and medicinal properties. Despite its widespread cultivation, there has been no documentation of plant viruses on gardenia yet. In the present study, gardenia leaves exhibiting symptoms of plant viral diseases were sampled and sequenced by both metatranscriptome and small RNA sequencing. As a consequence, bean common mosaic virus (BCMV) was identified in gardenia for the first time and named BCMV-gardenia. The full genome sequence of BCMV-gardenia is 10,054 nucleotides (nt) in length (excluding the poly (A) at the 3' termini), encoding a large polyprotein of 3,222 amino acids. Sequence analysis showed that the N-termini of the polyprotein encoded by BCMV-gardenia is less conserved when compared to other BCMV isolates, whereas the C-termini is the most conserved. Maximum likelihood phylogenetic analysis showed that BCMVgardenia was clustered closely with other BCMV isolates identified outside the leguminous plants. Our results indicated that the majority of BCMV-gardenia virus-derived small interfering RNAs (vsiRNAs) were 21 nt and 22 nt, with 21 nt being more abundant. The first nucleotide at the 5' termini of vsiRNAs derived from BCMV-gardenia preferred U and A. The ratio of vsiRNAs derived from sense (51.1%) and antisense (48.9%) strands is approaching, and the distribution of vsiRNAs along the viral genome is generally even, with some hot spots forming in local regions. Our findings could provide new insights into the diversity, evolution, and host expansion of BCMV and contribute to the prevention and treatment of this virus.

• Mycobiology
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• 제48권1호
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• pp.44-50
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• 2020

Calonectria pseudonaviculata and C. henricotiae are two closely related fungal species responsible for boxwood blight disease of ornamental shrubs (Buxus spp.) in the U.S. and Europe. A previous study has shown isolates of the latter species, which is restricted to Europe, to be less sensitive to tetraconazole, an azole fungicide. In this study, we have analyzed the CYP51 paralogs for polymorphism in 26 genomes, representing geographically disparate populations of C. pseudonaviculata (n = 19) and C. henricotiae (n = 7), from the U.S., Europe, Asia, and New Zealand. The presence of a CYP51A pseudogene and lack of a functional CYP51A paralog in all C. pseudonaviculata genomes examined is a novel discovery for fungi and could have implications for the evolution of resistance to antifungal chemicals.

• 대한화학회지
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• 제57권4호
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• pp.455-460
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• 2013

A novel and simple method for the synthesis of 2-amino-4H-pyran-3,4,5-tricarboxylate derivative and the evaluation of their antibacterial activity against Pseudomonas syringae, Xanthomonas citi and Pectobacterium carotovorum are reported. The structure of the isolated compounds has been determined by means of $^1H/^{13}C$ NMR and FT-IR Spectroscopy. The reaction of alkyl isocyanides with acetylenic esters in the presence of dimethyl acetone-1,3-dicarboxylate in the present of $BF_3.SiO_2$ at ambient temperature. Some of the compound showed significant inhibition to growth of bacteria.

• Mycobiology
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• 제51권3호
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• pp.122-138
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• 2023

Elsinoë are plant pathogenic fungi that cause scabs, spotted anthracnose, and some morphological distortions on various plants, including woody plants, economically important crops, and ornamental plants. Taxonomical reexamination of Elsinoë species in Japan has not yet been conducted based on the modern species criteria. In this study, several Japanese isolates were reexamine based on the morphological and molecular-phylogenetic analysis of the internal transcribed spacer region (ITS), large subunit gene (LSU)m and protein-coding gene such as RNA polymerase II subunit (rpb2) and Translation elongation factor 1-alpha (tef). Japanese isolates were divided into four clades and three new species, Elsinoë hydrangeae, E. sumire, and E. tanashiensis were proposed. One species, Sphaceloma akebiae, was transferred to the genus Elsinoë.

• 한국자원식물학회지
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• 제32권4호
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• pp.312-317
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• 2019

본 연구는 기존에 절화용으로 개발되지 않았던 암대극을 새로운 관상식물로 개발하기 위해 수행되었다. 실험은 보존용액에 따른 절화 암대극의 영향을 구명하고자 수분흡수율, 절화수명, 상대생체중을 조사하였다. 수확 후 절화는 8-hydroxyquinoline sulfate (8-HQS) 10, 50, $100mg{\cdot}L^{-1}$, silver thiosulfate (STS) 0.1, 0.2 mM, Chrysal, Floralife의 보존용액에 처리되었다. 실험은 온도 $22.6^{\circ}C$, 상대습도 45%, 일장 9/15h, 광도 $9.89{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ 환경에서 수행되었다. 절화 암대극의 수분흡수율은 8-HQS $10mg{\cdot}L^{-1}$ 보존용액에 처리 시 무처리된 절화보다 유의하게 높게 측정되었다. 그러나 절화 암대극의 수명은 무처리와 8-HQS $10mg{\cdot}L^{-1}$ 보존용액 처리 시 차이가 관찰되지 않았다. 절화 수명은 STS 보존용액 처리 시 처리농도가 증가함에 따라 감소하였다. 절화 암대극의 상대 생체중은 상업용 절화수명연장제인 Chrysal 및 Floralife 보존용액 처리 시 무처리보다 유의하게 감소하였다. 이러한 결과는 절화 암대극의 사용에 있어 유용한 자료가 될 것이다.