• Journal of Microbiology and Biotechnology
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• 제29권2호
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• pp.244-255
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• 2019

Xylose isomerase (XI; E.C. 5.3.1.5) catalyzes the isomerization of xylose to xylulose, which can be used to produce bioethanol through fermentation. Therefore, XI has recently gained attention as a key catalyst in the bioenergy industry. Here, we identified, purified, and characterized a XI (PbXI) from the psychrophilic soil microorganism, Paenibacillus sp. R4. Surprisingly, activity assay results showed that PbXI is not a cold-active enzyme, but displays optimal activity at $60^{\circ}C$. We solved the crystal structure of PbXI at $1.94-{\AA}$ resolution to investigate the origin of its thermostability. The PbXI structure shows a $({\beta}/{\alpha})_8$-barrel fold with tight tetrameric interactions and it has three divalent metal ions (CaI, CaII, and CaIII). Two metal ions (CaI and CaII) located in the active site are known to be involved in the enzymatic reaction. The third metal ion (CaIII), located near the ${\beta}4-{\alpha}6$ loop region, was newly identified and is thought to be important for the stability of PbXI. Compared with previously determined thermostable and mesophilic XI structures, the ${\beta}1-{\alpha}2$ loop structures near the substrate binding pocket of PbXI were remarkably different. Site-directed mutagenesis studies suggested that the flexible ${\beta}1-{\alpha}2$ loop region is essential for PbXI activity. Our findings provide valuable insights that can be applied in protein engineering to generate low-temperature purpose-specific XI enzymes.

• 대한건축학회논문집:계획계
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• 제34권9호
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• pp.87-96
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• 2018

The purpose of this study is to get a more fundamental definition of the term Architecture and Geonchuk through analyzing the origin and reception of the terminus technicus Architektonike. In Latin world the greek term Architektonike was adopted and replaced the term Constructio by Vitruvius and has played a significant role in the history of architecture. In the late 19th Century however, the word Architecture was translated into Geonchuk(建築) and has been dominantly used in countries such as Korea, Japan, and China. But the difference of the denotation between them has not yet been fully evaluated. The term Geonchuk signifies much the same as the Tektonik and remains as such in the architectural discourse. In this context, this study goes back to refer to the philosophy of Metaphysics by Aristotle. In his book the term Architektonike was defined for the first time as terminus technicus, and the philosophies in later Ages of Leibniz or of Kant adopted it to signify the world view in accordance with the time. However, the original thought of the Architektonike disappeared in architectural practice and has been used in most cases as a substitute for Oikodomike which denotes house-building. The usage of this word in later time shows that it lost its foundation to be interpreted not only in terms of principium cognoscendi, but also in terms of principium reale. From this the literal usage of the term architecture and Geonchuk has seldom to do with its original meaning and denotation of Architektonike and also they lost the status of terminus technicus for the discourse of arche which encompasses the meaning of both principle and universal order.

• 대한건축학회논문집:계획계
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• 제34권12호
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• pp.135-144
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• 2018

This paper deals with preservation and restoration as a universal approach to conserve architectural heritage. The questions on how to preserve or restore them have been always major issues for many old buildings. Reading changes in ways of the thinking to solve such matters in the past helps to grasp the fundamental concepts to conserve cultural heritage at this point in time. The method is an important stage that leads to change our current attitude. Both the ways of the thinking for preservation and restoration should be re-interpreted to preserve memory or to restore identity depending on the current situation, and even should no longer be understood as two opposite options. Therefore, this paper focuses on the epistemological notion and reveals the origin and premise of modern historical perception that has become disconnected from the past works. By taking the writings of $Eug{\grave{e}}ne$-Emmanuel Viollet le Duc, John Ruskin, and Camillo Boito into consideration, the thesis shows that their thought, in the common denominator of the time, is a kind of reflection of consciousness according to particular historical contexts and that their ideas echo three dialectical paradigms derived from past and present, memory and forgetfulness, and history and truth.

• 현장농수산연구지
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• 제24권1호
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• pp.36-40
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• 2022

The purpose of this study was to evaluate hippological differences between Jeju ponies and Jeju Pony crossbreds population. This is the first repot of the differences use an anhidrosis in Korean native horses. The precise prevalence of anhidrosis is unknown; however, it has been estimated that 6-20% of horses may be affected. However, there is no report about the incidence of the disease in pony breeds. We performed diagnosis by clinical signs (sweating) to investigate the incidence of anhidrosis in Jeju Ponies(n=340) and Jeju Pony crossbreds (n=536) at Jeju Race Park from July to September in 2012 as a way of find of hippological difference. Results of this study showed that 74 (21.8%) of the 340 examined Jeju Ponies and 61 (11.4%) of the 536 examined Jeju Pony crossbreds had anhidrosis. So, the former had almost two times higher than the later. Among 74 Jeju Ponies those had the disease, 50 were male (23.1%) and 24 were female (19.4%). Among 61 Jeju Pony crossbreds those had the disease, 22 were male (9.5%) and 39 were female (12.8). In Jeju Ponies, anhidrosis were most common in above the age of 5, followed by 4, 2, 3-years-old. For Jeju Pony crossbreds, the disease were most common in 2-years-old, followed by above the age of 5, 4, 3-years-old. In two breeds, 3-year-old animals were most rare respectively. There was no predilection of age and sex which is correlated with another study. In conclusion, the incidence of anhidrosis in the ponies were considerably similar foreign countries. However, the authors thought that causes of the difference of the breeds were origin and genetic differences.

• 한방재활의학과학회지
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• 제32권2호
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• pp.139-154
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• 2022

Objectives This study was conducted to confirm anatomical information near the treatment areas of visceral chuna manual therapy and to secure stability and effectiveness during the treatment. Methods For 50 healthy adult men and women, a total of 13 ultrasound images were taken of the 7 treatment areas which are the representative treatment areas of visceral Chuna manual therapy. Results The treatment area of the bottom of the liver can be palpated around the right side ST19 and CV14. The treatment area of the gallbladder and the common bile duct can be palpated around the right side SP16 and ST20. The treatment area of the cardia and the pylorus can be palpated around the left side KI21, right side ST20, ST21, KI19, KI20, CV12, and CV13. The origin point of the mesentery root can be moved to the left and lower left from CV12 and can be palpated. The treatment area of the ileocecal valve and the cecum can be palpated around the right side SP14. The treatment area for the colic flexure can be palpated around the both side LR13. The treatment support point for the kidneys can be palpated around both side BL51. Conclusions It is thought that if the ultrasound image data from the meridians around the treatment site is used as an auxiliary, it will be more effective in terms of safety and effectiveness during the treatment of visceral Chuna manual therapy.

• 한국자연치유학회지
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• 제10권1호
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• pp.56-65
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• 2021

목적: 본 연구는 아유르베다 사상을 통해 현대 서양 과학과 철학의 한계의 원인을 고찰하고, 치유사업 발전을 위한 홀리즘의 길을 모색하고자 하였다. 이에 현대 서양 문명이 오늘의 한계에 이르게 된 배경과 치유사업의 현실이 그맥을 함께 할 것이라 추론하였다. 방법: 이를 살펴보기 위해 동서양의 '몸과 마음에 대한 인식'의 차이와 '존재의 근원에 대한 관점'의 차이를 살펴보고 이에 따른 아유르베다 5원소설의 특징을 살펴보았다. 결과: 그 과정을 통해 서양의 '심신이원론'과 서양 4원소설에서의 '공(空)요소'의 부재'를 한계의 원인으로 지목하게 되었다. '공(空)요소'가 보이지 않는 세계, 비물질의 세계라는 점에서 공의 부재가 서양 문명의 한계인 동시에 국가 치유사업 현실의 문제라고 결론짓게 되었다. 결론: 본 연구가 아유르베다 사상의 지속적인 연구와 자연치유 분야의 연구로 이어져 치유사업의 발전의 기초정보가 되고, 국민의 건강과 웰빙·자기치유를 기대하여 본다.

• Korean Journal of Acupuncture
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• 제40권1호
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• pp.13-17
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• 2023

Objectives : Our goals were to examine how the well points in the fingers came to be and how their placements have changed, as well as to determine how they relate to the EX-UE11 points. Methods : We reviewed the classic textbooks to understand the origin and the changes of locations of the well points in the fingers. We also compared the location and indications between well points in the fingers and EX-UE11 points. Results : At first, the tips of the fingers, which are now thought to be the locations of EX-UE11 points, were once described as containing well points. Currently, well points are positioned 0.1 F cun distal-medial (or lateral) to the medial (or lateral) corner of the nail. In addition to the locational commonality, we found similarities between the well points in the fingers and the EX-UE11 points in terms of their indications; for example, bloodletting at these places is frequently utilized to treat emergencies, including acute stroke and fever. Conclusions : We suggest that it is highly likely that well points in the fingers and EX-UE11 points were initially the same acupuncture point and later classified into two different acupuncture points, given their identical locations and indications. If the clinical relevance between the change process of the well points' locations in the fingers and the EX-UE11 is studied in the historical literature, it is anticipated that the significance and clinical application of well points can be expanded.

• 현장농수산연구지
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• 제25권4호
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• pp.39-43
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• 2024

Little research has been done on investigating the specific characteristics of Jeju Ponies (Korean native horse) and Jeju Pony crossbreds. The authors performed evaluation of skin thickness of the skin of these breeds (n=205) at Jeju Race Park as a way of find of hippological difference. Results of this study showed that the Jeju Pony's skin were thicker than the Jeju Pony crossbred. The male's skin were thicker than the female's skin in two breeds respectively. Especially, in the Jeju Pony show significant differences (P < 0.05). In Jeju Pony crossbreds, as the skin thickness were thinner with age increase. 2-year-old group's skin significantly thinner than 3 and over 4-year-old groups. However, the Jeju Pony's skin were not significantly different by age. In conclusion, the authors thought that causes of the difference of the breeds were origin and genetic differences among the horse and the pony. Furthermore, the modified umbilical herniorrhaphy with rubber rings must be used cautiously in the Jeju Pony. It should be taken into account the animals' skin thickness when the leather goods created by using their skin.

• 한국식품영양과학회지
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• 제39권5호
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• pp.709-718
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• 2010

본 조사 연구는 경남, 부산지역 및 대구, 경북지역의 한우 소비자 400명을 대상으로 설문조사를 통하여, 한우의 구매 특성과 소비자 만족도를 조사였다. 불충실하게 응답한 37부를 제외하고 총 363부를 선정하여 통계 분석한 결과는 다음과 같다. 설문조사에 대한 응답자들의 성별은 남성 107명(29.5%), 여성 256명(70.5%)이었으며, 연령은 20대 70명(19.3%), 30대 97명(26.7%), 40대 131명(36.1%), 50대 이상 65명(17.9%)이었다. 한우 구매횟수는 연령이 많을수록 구매횟수가 더 많은 것으로 나타났으며(p<0.05), 한우 구입처는 농축협 한우전문점(54.5%)이 가장 많았다(p<0.05). 한우의 구매 부위는 등심(43.5%), 갈비(22.9%), 안심(10.5%), 양지(9.9%), 우둔 사태(4.7%), 부산물, 기타(3.3%), 목심(2.8%), 채끝 설도(2.5%) 순으로 나타났다. 수입쇠고기의 구매경험에서는 '가끔 있다'와 '있다'가 각각 24.55%와 22.3%를 나타내었으며, 그 이유로 '가격이 싸서'(73.3%)가 대부분이었다. 수입쇠고기의 둔갑판매에 대하여서는 '가끔 그렇다(69.1%)'로 응답하였으며, 이에 대한 예방책으로 쇠고기생산이력추적시스템(61.7%), 쇠고기원산지표시제(17.1%) 순으로 나타났다. 전반적으로 쇠고기생산이력추적시스템과 원산지표시제 도입에 따른 소비자 신뢰도가 70% 이상이라고 볼 때, 이에 응답율은 각각 61.1%와 75.5%로 나타났다(p<0.05). 한우 브랜드 육으로서 성장하기 위한 선행요인은 한우고기의 맛(3.90점), 고객만족도(3.20점), 안전성(3.20점), 고객신뢰도(3.20점)순위로 나타났다. 한우에 대한 전반적인 소비자의 안전성은 학력(p<0.05)과 직업(p<0.01)에 따라 크게 달랐으며, 고객만족도는 연령과 학력, 직업에 큰 영향을 받는 것으로 나타났고(p<0.05), 고객신뢰도는 학력과 수입, 직업에 크게 영향을 받는 것으로 나타나 통계적인 차이를 나타내었다(p<0.05). 이상의 결과를 종합해볼 때, 한우소비자들은 수입쇠고기의 둔갑판매에 대한 우려가 높은 편이지만 쇠고기생산이력추적시스템 제도와 쇠고기원산지표시제가 올바르게 정착될 수 있도록 정부차원의 지속적인 교육과 관리감독이 이루어진다면, 소비자들의 고객만족과 신뢰도는 높아질 것으로 판단되며, 한우 브랜드 육으로 성장하기 위해서는 맛과 안전성이 가장 중요한 것으로 판단된다.

• 동국한의학연구소논문집
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• 제6권1호
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• pp.1-34
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• 1997

동양의학에서 "상한론(傷寒論)"의 발전은 대체로 삼단계(三段階)의 과정을 거쳤으나 "삼강정립(三綱鼎立)"설이 대세를 이루고 있었다. 이러한 관점에 대항하여 변증논치규율(辯證論治規律)을 연구한 학파(學派)가 나타나게 되었는데 우이(尤怡)가 그 중 한사람이다. 우이(尤怡)의 생애(生涯), 저서(著書), 학술사상(學術思想) 및 후세에 미친 영향 등을 조사하고 특히 "상한론(傷寒論)"을 안법류증(按法類?)하여 육경(六經)에 따른 정치법(正治法) 권변법(權變法) 알선법(斡旋法) 구역법(救逆法) 유병법(類病法) 명변법(明辨法) 잡치법(雜治法) 등의 치법(治法)에 대하여 연구하여 보고하는 바이다.