• Title/Summary/Keyword: origin identification

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First Morphological Description of Ophichthus asakusae (Ophichthidae: Anguilliformes) Leptocephali Collected from the Waters South of Jeju Island (제주도 남방해역에서 채집된 제주바다뱀(Ophichthus asakusae) 엽상자어(Leptocephalus)의 첫 형태기재)

  • Ji, Hwan-Sung;Choi, Jung Hwa;Choi, Kwang Ho;Lee, Dong Woo;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.46 no.5
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    • pp.594-597
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    • 2013
  • Two leptocephali [total length (TL) 68.2-80.5 mm] from the family Ophichthidae, collected from the waters south of Jeju Island, Korea, were identified using morphology. The two ophichthid leptocephali were identified as belonging to the genus Ophichthus based on morphological characters: 131-132 myomeres; nine gut swellings; dorsal fin origin above the middle of the body; five postanal pigments present from the anus to the caudal margin. Ultimately, both leptocephali were identified as Ophichthus asakusae, as the total number of myomeres was consistent with those of adult O. asakusae. Here, we first describe the morphological characteristics of the O. asakusae leptocephalus, and suggest diagnostic characters for leptocephali belonging to the genus Ophichthus.

Identification of the Capsid Protein-binding Region of the SL1(+) RNA Located at the 5' Region of the Potato virus X Genome

  • Cho, Sang-Yun;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.28 no.1
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    • pp.75-80
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    • 2012
  • $Potato$ $virus$ $X$ (PVX) contains $cis$-acting elements including stem-loop 1 (SL1) RNA at the 5' region; SL1 is conserved among all potexviruses. The SL1 at the positive-sense RNA, SL1(+), is required for PVX RNA replication, cell-to-cell movement, and translation. Previous research demonstrated that SL1(+) RNA also serves as the origin of assembly for encapsidation of PVX RNA. To identify the essential sequences and/or regions for capsid protein (CP) subunit recognition within SL1(+) RNA, we used electrophoretic mobility shift assays (EMSA), UV cross-linking, and yeast three-hybrid analyses. The EMSA and UV cross-linking analyses with PVX CP subunits and RNA transcripts corresponding to the SL1(+) RNA showed that the SL1(+) RNA formed complexes with CP subunits. We also conducted EMSA and yeast three-hybrid analyses with RNAs containing various mutations of SL1(+) RNA elements. These analyses indicated that SL1(+) RNA is required for the interaction with PVX CP and that the RNA sequences located at the loop C and tetra loop of the SL1(+) are crucial for CP binding. These results indicate that, in addition to being important for RNA accumulation, the SL1(+) RNA from the 5' region of the PVX genome is also required for specific binding of PVX CP.

Multidrug resistance of coagulase-negative staphylococci isolated from rescued wild animals

  • Rhim, Haerin;Kim, Hong-Cheul;Na, Ki-Jeong;Han, Jae-Ik
    • Korean Journal of Veterinary Service
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    • v.42 no.4
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    • pp.251-255
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    • 2019
  • Wildlife is a bio-indicator of environmental pollution by antimicrobial resistant bacteria or genes, however, there is no information on antimicrobial resistance in wildlife-origin bacteria. This study aimed to investigate the normal microbiota of staphylococci and their antimicrobial resistance in wildlife that did not take any antimicrobials. After sampling and bacterial isolation/identification, antimicrobial resistance profiles were examined by broth microdilution test, Kirby-Bauer disc diffusion test and mecA genetargeted PCR. Of 90 isolates from wildlife, 83 were coagulase-negative staphylococci while only 7 were coagulase-positive staphylococci. Methicillin-resistance was found in 63 (70%) isolates and 35 of 90 (38.9%) isolates were multidrug-resistant staphylococci. When considering that all of the animals did not take any medication or contacted any medical device before the sampling, the results indicate significantly high prevalence of antimicrobial resistance in wild environments. Further study would be necessary to investigate the transmission route of antimicrobial resistance.

Effect of Particulate Matter on Human Health, Prevention, and Imaging Using PET or SPECT

  • Zaheer, Javeria;Jeon, Jongho;Lee, Seung-Bok;Kim, Jin Su
    • Progress in Medical Physics
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    • v.29 no.3
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    • pp.81-91
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    • 2018
  • Particulate matter (PM) in dust causes serious pathological conditions, and it has been considered a critical health issue for many years. Respiratory disorders such as bronchitis, asthma, and chronic inflammation, are the most common illnesses due to PM that appears as dust. There is evidence that cardiovascular and neurological abnormalities are caused by PM. Although an extensive amount of work has been conducted on this topic, including studies on the nature of the particles, particle size measurements, particle distribution upon inhalation, the health effects of fine particles, disease prevention, diagnosis, and treatment, to this date, there is still a considerable lack of knowledge in these areas. Therefore, the identification of the key components that cause diseases owing to PM, and the specific diagnoses of the diseases, is important. This review will explore the current literature on the origin and nature of PM and their effects on human health. In addition, it will also highlight the approaches that have been adopted in order to diagnose the effects of PM using positron emission tomography (PET) or single-photon emission computerized tomography (SPECT).

Persistent Trigeminal Artery Detected by Conventional Angiography and Magnetic Resonance Angiography

  • Kim, Myoung-Soo;Hur, Jin-Woo;Lee, Jong-Won;Lee, Hyun-Koo
    • Journal of Korean Neurosurgical Society
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    • v.37 no.2
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    • pp.101-104
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    • 2005
  • Objective: A persistent trigeminal artery (PTA) may be found incidentally on conventional cerebral angiography and magnetic resonance(MR) angiography. Our goal is to examine the course and relationships of the vessel to the surrounding structures. Methods: Cerebral angiography was performed in 494 patients and MRA in 880; the patients had or were suspected to have cerebrovascular disease. In the images, the incidence, origin, course, and relationships of the PTA were evaluated. Results: A PTA was found in two (0.4%) of the patients undergoing cerebral angiography and three (0.34%) receiving an MR angiography. In four patients, the PTA arose from the lateral part of the cavernous segment of the internal carotid artery, then passed caudally and around the base of the dorsum sellae. In the other patient, the PTA arose from the medial aspect of the siphon, and ascended sharply to pierce the dorsum sellae and join the basilar artery. In four cases, there was hypoplasia of a proximal basilar artery below the abnormal communication; the vessel was of increased diameter above the communication. Conclusion: Identification of a PTA with a trans-sellar course is crucial if trans-sphenoidal surgery is planned. Hypoplasia of a proximal basilar artery should not be mistaken for an acquired narrowing.

Identification of 26 Germplasms of Safflower (Carthamus tinctorius L.) with ISSR and SCAR Markers

  • Sung, Jung-Sook;Cho, Gyu-Taek;Lee, Suk-Young;Baek, Hyung-Jin;Park, So-Hye;Huh, Man-Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.4
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    • pp.319-326
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    • 2010
  • Safflower (Carthamus tinctorius L.) is a herb primarily distributed throughout in the world. We have used the inter-simple sequence repeats (ISSR) technique to investigate the phylogenetic relationships and genetic diversity of C. tinctorius. Of all germplasms, 88.7% were polymorphic among all germplasms. Mean genetic diversity within germplasms was very low (0.048). The Turkey germplasm had the highest expected diversity (0.082) and Australia germplasm was the lowest (0.020). These values indicate that most of the genetic diversity of safflower is found among germplasms and there is a high among-germplasm differentiation. We found eight phenetic bands for determining the specific marker of germplasm with SCAR markers. The regions of the Mediterranean Sea and India may be the most probable candidates for the origin of safflower. The tree showed four major clades: (1) European germplasms, (2) Azerbaijan, Egypt, and Ethiopia, (3) Australia, and (4) America.

Human-ware Approach on the Difference between Industrial Relations of Korea and Japan -Based on the Identification of the Gene- (한국과 일본의 기업풍토 및 경영성과 차이에 대한 휴먼웨어적 접근 -동일 유전자 측면을 기초로 하여-)

  • 이철우;서윤정
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.23 no.55
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    • pp.97-106
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    • 2000
  • In this paper, we would be searching for the complemental aspects in industrial relations of Korea compared with that of Japan. It is very reasonable that historical, geographical, and especially gene analysis data are almost identical, considering that the general evaluations to the motive of Japan's industry are $\ulcorner$the modernization of the tradition$\lrcorner$. Although it is reported Korea accomplished the high economic growth rate, the economic growth rate(efficiency) could not be continued. This reason looks like that Korean proper(eigen) traits(:Gene-ware) have been oppressed and concealed by social climate such as jealousy, stratagem and hatred, we can find one of this facts in the criminal and complaint numbers as social trust measurement. There are many cases that Korean industry was distorted by the superiority/inferiority relations, for examples, the loss of the native(eigen or proper) resources, a distorted introduction of the production method and the unfolding of the industry which be not related to the characteristics of region. In this reasons, Korean industry could not play the role of Human-ware function which activates Gene-ware or, Gene-Nascence. In this Human-ware aspects, Japanese characteristics, such as $\ulcorner$Getting the basic right$\lrcorner$ and $\ulcorner$Tendency to the origin$\lrcorner$, are to be useful in the Nascence of Gene-ware in Korean industry.

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Identification of Soybean Glycinin Precursor In Vitro (대두 세포내에서 Glycinin 전구체의 존재 확인)

  • 김정호
    • Journal of Plant Biology
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    • v.32 no.1
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    • pp.51-65
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    • 1989
  • Glycinin is the major storage protein in soybean. It has been known that a molecule of glycinin is composed of 6 subunits, each of which consists of two different kinds of polypeptides, acidic (A) and basic (B) one (NW 39K and 19K, respectively). To study the molecular origin and the relationship of glycinin subunit polypeptides, antibodies against A-and B-polypeptide were obtained by immunizing rabbits with either of the antigens purified by gel filtration and preparative electrophoresis. Each antibody was not only specific for its own antigen polypeptide in soybeans but also recoginzed the precursor which was synthesized in vivo and in vitro. The polyadenylated mRNAs were isolated from immature seeds and leaves and were translated in vitro using wheat germ extract. One of the seed-specific translation products. MW 60K, was identified to be the precursor of glycinin subunit by immunoprecipitation with antibodies against glycinin A- and B-polypeptide. Mature A- and B-polypeptides were not detected in the translte in vitro. These results suggest that the precursor polypeptide is synthesized from the mRNA and is cleaved to yield A- and B-polypeptides which from a glycinin subunit in the cell. Glycinin genes were expressed with the maturation of soybean seeds in a tissue-specific and developmental stage-specific manner.

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Characterization of the complete mitochondrial genome of Mauritian sardinella, Sardinella jussieu (Lacepède, 1803), collected in the Banten Bay, Indonesia

  • Sektiana, Sinar Pagi;Andriyono, Sapto;Kim, Hyun-Woo
    • Fisheries and Aquatic Sciences
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    • v.20 no.10
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    • pp.26.1-26.9
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    • 2017
  • Fishes in genus Sardinella are small pelagic species, which plays an important role in marine ecosystem as the first consumer. Those species are also commercially important, whose total catch reaches 278,600 tons in 2011 in Indonesia, but their identification has been difficult for their morphological similarity. In this study, we reported Sardinella jussieu for the first time in Indonesian coastal area (Banten Bay, Indonesia, $6^{\circ}\;0^{\prime}\;50.00^{{\prime}{\prime}}\;S-106^{\circ}\;10^{\prime}\;21.00^{{\prime}{\prime}}\;E$). We were able to confirm the species by both its morphological characteristics including the black spot at dorsal fin origin, the dusky pigmentation at caudal fin, 31 total scute numbers, and DNA sequence identity in the GenBank database by the molecular analysis. Its total mitochondrial genome was determined by the combination of next-generation sequencing and typical PCR strategy. The total mitochondrial genome of Sardinella jussieu (16,695 bp) encoded 13 proteins, 2 ribosomal RNAs, 22 transfer RNAs, and the putative control region. All protein-coding genes started with ATG and typical stop codon and ended with TAA or TAG except for ND4 in which AGA is used. Phylogenetic analyses of both COI region and full mitochondrial genome showed that S. jussieu is most closely related to Sardinella albella and Sardinella gibbosa

Understanding Giftedness in a Cognitive Mechanism: A Candidate for a Universally Agreed Definition of Giftedness (인지메카니즘 내에서의 영재성의 이해: 보편적이고 통일된 새로운 영재에 대한 정의)

  • Song, Kwang-Han
    • Journal of Gifted/Talented Education
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    • v.19 no.2
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    • pp.261-277
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    • 2009
  • This article provides a new definition of giftedness drawn from a cognitive mechanism. The mechanism shows how cognitive components are functionally related to each other and cooperatively "work" together, producing the various cognitive phenomena in the social world. The author argues that for a universally agreed definition, giftedness has to be understood in the mechanism, the origin of all the cognitive phenomena in cultural or social contexts. According to the definition drawn form the cognitive mechanism, giftedness is the ability to form a simple and fundamental domains-integrated knowledge of the "whole" world. A new method for identifying gifted students is subsequently suggested.