• KSBB Journal
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• 제11권5호
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• pp.565-571
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• 1996

Aspergillus oryzae A Tee 2114를 사용하여 $\beta$-mannanase의 생산에 영향을 주는 배지성분의 최적 화를 수행하였다. 탄소원인 locust bean gum의 농 도를 달리하여 발효를 수행하였다. Locust bean gum의 농도가 20 g/L 이상일 때는 초기의 점도가 높아 혼합이 거의 되지 않아 초기에 $\beta$-mannanase 의 생산이 지 연되는 현상이 나타났고. Locust bean g gum의 농도가 증가할수록 $\beta$-mannanase의 역가와 균체농도가 비례척으로 증가하였다. Locust bean gum 10 g/L 배지에서 여러 가지 질소원이 $\beta$-mann anase의 생산에 미치는 영향을 살펴 본 결과,B-man n nanase의 역가는 무기질소원 중에서는 $(NH-4)_2SO_4$가 좋았으며 유기질소원 중에서는 malt extract가 가장 좋았다. 여러 가지 무기엽의 최적화를 수행한 결과 KH,PO.가 $\beta$-mannanase의 생산에 중요한 인자임을 알 수 있었다. 배지최적화 결과 최적배지 로 locust bean gum 10 giLt malt extract 3 g/L, $(NH-4)_2SO_4 2 g/L, KH_2PO_4$ 10 g/L이 결정되었으며 이때 $\beta$mannanase의 역가는 거의 6 unit/mL에 접 근하였다. 최적배지를 사용하여 발효조에서 배양을 수행하였다. 발효조의 흔합효과로 배양초기에 나타 나는 $\beta$-mannanase 생산 지 연현상을 감소시 킬 수 있었고 배양시간도 단축할 수 있었다. 27시간 배양 한 후 $\beta$mannanase의 역가 9.7 unit/mL와 비 $\beta$-mannanase의 역가 1.9 unit/mg-cell-을- 얻었다. 이 때, 생산성은 0.36 unit/mL. h이었다.

• 한국산림과학회지
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• 제101권4호
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• pp.640-647
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• 2012

잣나무(Pinus koraiensis) 4년생의 생장특성인자와 관련이 있는 주요 대사물질을 확인하기위한 대사체 분석을 실시하였다. GC/MS로 분리한 105종을 대상으로 길이생장 및 건중량의 생장 특성과의 상관이 있는 대사물질을 분석하였다. Alanine, threonine, oleic acid 및 butanoic acids는 길이생장 및 건중량과 고도의 부의 상관관계가 있었고, 반면 malic acid, xylose, glucose, inositol 및 sucrose는 생장특성과 정의 상관관계가 있었다. 생장이 왕성한 시기에는 생장이 우수한 그룹에서 주지의 아미노산류나 유기산류 함량이 줄어드는 것으로 추정되었으며, 반면 단당류나 sucrose 같은 광합성 산물은 중위 및 하위 그룹에 비교해서 함량이 높은 것으로 나타났다. 특이하게도 박테리아나 균류에서 생성되는 d-turanose의 함량이 4년생 잣나무 묘고생장과 고도의 상관관계(r=0.896, p<0.001)가 있었으며, 이는 근균류와의 관계가 있는 것으로 추정된다. 이러한 인자들은 잣나무 우량개체 선발을 위한 대사 표지자 개발의 기초자료로 이용될 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권10호
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• pp.1513-1520
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• 2014

The present aim was to investigate the effects of traditional Chinese medicine prescriptions (TCM) on body temperature, blood physiological parameters, nutrient apparent digestibility and growth performance of beef cattle under heat stress conditions. Twenty-seven beef cattle were randomly divided into three groups as following; i) high temperature control (HTC), ii) traditional Chinese medicine prescriptions I+high temperature (TCM I) and iii) traditional Chinese medicine prescriptions II+high temperature (TCM II) (n = 9 per group). The results showed that the mean body temperature declined in TCM II treatment (p<0.05). Serum $T_3$ and $T_4$ levels with TCM I and TCM II treatments elevated (p<0.05), and serum cortisol levels of TCM I treatments decreased (p<0.05), compared with the HTC group. Total protein, albumin, globulin in TCM II treatments elevated and blood urea nitrogen levels of both TCM treatments increased, but glucose levels of both TCM treatments decreased, compared with the HTC group (p<0.05). The apparent digestibility of organic matter and crude protein with TCM I treatment increased, and the apparent digestibility of acid detergent fiber elevated in both TCM treatments (p<0.05). Average daily feed intake was not different among three groups, however average daily gain increased and the feed:gain ratio decreased with both TCM treatments, compared with the HTC group (p<0.05). The present results suggest that dietary supplementation with TCM I or TCM II improves growth performance of heat stressed beef cattle by relieving heat stress responses and increasing nutrient apparent digestibility.

• Asian-Australasian Journal of Animal Sciences
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• 제26권4호
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• pp.529-536
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• 2013

The objective of this study was to investigate the effect of levels of cottonseed meal with various carbohydrate sources in concentrate on feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in dairy bulls. Four, 6 months old dairy bulls were randomly assigned to receive four dietary treatments according to a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design. Factor A was carbohydrate source; cassava chip (CC) and cassava chip+rice bran in the ratio of 3:1 (CR3:1), and factor B was cotton seed meal levels in the concentrate; 109 g CP/kg (LCM) and 328 g CP/kg (HCM) at similar overall CP levels (490 g CP/kg). Bulls received urea-lime treated rice straw ad libitum and were supplemented with 10 g of concentrate/kg BW. It was found that carbohydrate source and level of cotton seed meal did not have significant effects on ruminal pH, ammonia nitrogen concentration, microbial protein synthesis or feed intake. Animals which received CC showed significantly higher BUN concentration, ruminal propionic acid and butyric acid proportions, while dry matter, organic matter digestibility, populations of total viable bacteria and proteolytic bacteria were lower than those in the CR3:1 treatment. The concentration of total volatile fatty acids was higher in HCM than LCM treatments, while the concentration of butyric acid was higher in LCM than HCM treatments. The population of proteolytic bacteria with the LCM treatments was higher than the HCM treatments; however other bacteria groups were similar among the different levels of cotton seed meal. Bulls which received LCM had higher protein digestibility than those receiving HCM. Therefore, using high levels of cassava chip and cotton seed meal might positively impact on energy and nitrogen balance for the microbial population in the rumen of the young dairy bull.

• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1619-1626
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• 2018

Objective: This study was an attempt to investigate the effect of levels of fungal (Lentinus sajor-caju) treated oil palm fronds (FTOPF) on digestibility, rumen fermentation, and nitrogen balance in goats. Methods: Four 16 month old male crossbred (Thai Native${\times}$Anglo Nubian) goats with initial body weights of $33.5{\pm}1.7kg$ were randomly assigned according to a $4{\times}4$ Latin square design. Four levels of FTOPF were assigned for feed intake. The experimental treatments consisted of 0%, 33%, 67%, and 100% of oil palm fronds (OPF) being replaced by FTOPF. Results: The results revealed that total dry matter intake and nutrient intake were not influenced (p>0.05) by the inclusion of FTOPF. However, the efficiency values of the digestibility of the dry matter, organic matter, crude protein, neutral detergent fiber, acid detergent fiber, and acid detergent lignin on FTOPF were higher (p<0.05) in treatments with 33%, 67%, and 100% of FTOPF compared with 0% of FTOPF. FTOPF feeding did not change the rumen pH, temperature, and $NH_3-N$. However, the FTOPF levels did affect the total volatile fatty acid (VFA), molar proportion of acetate, propionate, butyrate, ratio of acetic (propionic acid and acetic) plus butyric (propionic acid), and production of $CH_4$. The totals of VFA and propionate was lower in goat fed with 0% of FTOPF than in those of the other groups (p<0.05). The amount of nitrogen retention based on g/d/animal or the percentage of nitrogen retained was the lowest the goat fed with 0% of FTOPF (p<0.05), whereas nitrogen intake, excretion, and absorption were not changed among treatments. Conclusion: Based on this study, FTOPF could be effectively used as an alternative roughage source in total mixed ration diets, constituting at least up to 100% of OPF.

• 한국수산과학회지
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• 제49권3호
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• pp.301-309
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• 2016

To facilitate the effective use of butter clam shell as a natural calcium resource, we determined the optimal conditions for calcium lactate (BCCL) preparation with high solubility using response surface methodology (RSM). The polynomial models developed by RSM for pH, solubility and yield were highly effective in describing the relationships between factors (P<0.05). Increased molar ratios of calcined powder (BCCP) from butter clam shell led to reduced solubility, yield, color values and overall quality. The critical values of multiple response optimization to independent variables were 1.75 M and 0.94 M for lactic acid and BCCP, respectively. The actual values (pH 7.23, 97.42% for solubility and 423.22% for yield) under optimization conditions were similar to the predicted values. White indices of BCCLs were in the range of 86.70–90.86. Therefore, organic acid treatment improved color value. The buffering capacity of BCCLs was strong, at pH 2.82 to 3.80, upon the addition of less than 2 mL of 1 N HCl. The calcium content and solubility of BCCLs were 6.2–16.7 g/100 g and 93.6-98.5%, respectively. Fourier transform analysis of infrared spectroscopy data identified BCCL as calcium lactate pentahydrate, and the analysis of microstructure by field emission scanning electron microscopy revealed an irregular form.

• 한국대기환경학회지
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• 제33권2호
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• pp.87-96
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• 2017

In this study, 4 gases containing typical chlorinated volatile organic compounds (VOCs) were treated by ultraviolet (UV) irradiation. The typical chlorinated VOCs are dichloromethane (DCM), trichloromethane (TCM), carbon tetrachloride (CTC) and trichloroethylene (TCE). The removal efficiency (RE) and the products of chlorinated VOCs by UV irradiation are investigated. At this time, 2 types of background gas (air and nitrogen) were used to figure out the RE by photooxidation and photolysis. The specification of UV-lamp used in this study was low-pressure mercury lamp emitting wavelength of 185~254 nm. The experimental conditions were set as initial VOC concentration of $180{\pm}10ppm$, empty bed retention time (EBRT) of 53 s, temperature of $23{\pm}2^{\circ}C$ and relative humidity of $65{\pm}5%$. In the photolysis condition with nitrogen ($N_2$) as background gas, the averaged RE of the 4 types of chlorinated VOCs was about 24% higher than that with photooxidation; and the REs of VOCs except CTC were confirmed as >99%. The composition of off-gases after UV photooxidation in air was investigated and several intermediates from DCM, TCM and TCE were detected by GC/MS. Among them, phosgene which is a toxics was detected as an intermediate of TCM. In addition, the concentration of carbon dioxide ($CO_2$) in the off-gases was measured to calculate the mineralization rate (MR). With the photooxidation, TCE showed the highest RE (>99%) while MR was the lowest (17%); and the MR of DCM was the highest (86%). In addition, particulate matters (PM) in the off-gases was also detected and high concentrated $PM_{10}$ ($21,580{\mu}g{\cdot}m^{-3}$) and $PM_{2.5}$ ($6,346{\mu}g{\cdot}m^{-3}$) were detected in TCE off-gas. More than 99% of the chlorinated VOCs could be removed using UV254-185 nm lamp, while it is necessary to conduct further studies on the production and treatment of secondary pollutants.

• 한국산림과학회지
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• 제95권4호
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• pp.423-428
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• 2006

녹화식물의 조기녹화와 자연식생으로의 천이를 빠르게 유도하기 위하여 임도 비탈면에 미생물제재의 사용을 통한 산림토양의 개선효과 및 녹화식물의 초기생육 상태를 분석한 결과는 다음과 같다. 토양의 pH는 미생물 처리구에서 5.69로 많이 중화되어 약산성을 보였고, 치환성 양이온도 대조구에서의 1.59(me/kg)보다 처리구에서 비교적 높은 수치인 5.04(me/kg)로 나타났다. 전질소함량(T-N) 및 유기물 함량도 처리구에서 훨씬 높게 나타나 산림토양의 비옥도가 아주 높게 개선되었음을 알 수 있었다. 미생물제재로 처리된 실험구의 평균 피복율은 대조구보다 2.5배 높게 나타났으며, 각 소형방형구의 발아 평균본수는 미생물 처리구가 대조구보다 2.7배나 높은 발생율을 보였다. 처리구별 목본인 참싸리의 초기 평균 생장량의 차이는 없는 것으로 나타났으며 참싸리의 초장은 발아 초기에 빠른 신장을 보이므로 외래초종과 혼파하면 사면안정 및 경관보전에 유리함을 알 수 있었다. 따라서 현재 외래종 위주의 단순한 종자뿜어붙이기공법의 녹화개념에서 초본 및 목본식물을 혼용한 외래종과 재래종의 사용과 미생물제재를 이용한 미생물 녹화공법의 도입은 산림토양의 개선과 사면안정 및 경관보전 효과를 높이면서 임도 비탈면의 조기녹화에 크게 기여할 수 있을 것으로 기대된다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.362-362
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• 2014

Atomic layer deposition (ALD) can be regarded as a special variation of the chemical vapor deposition method for reducing film thickness. ALD is based on sequential self-limiting reactions from the gas phase to produce thin films and over-layers in the nanometer scale with perfect conformality and process controllability. These characteristics make ALD an important film deposition technique for nanoelectronics. Tantalum pentoxide ($Ta_2O_5$) has a number of applications in optics and electronics due to its superior properties, such as thermal and chemical stability, high refractive index (>2.0), low absorption in near-UV to IR regions, and high-k. In particular, the dielectric constant of amorphous $Ta_2O_5$ is typically close to 25. Accordingly, $Ta_2O_5$ has been extensively studied in various electronics such as metal oxide semiconductor field-effect transistors (FET), organic FET, dynamic random access memories (RAM), resistance RAM, etc. In this experiment, the variations of chemical and interfacial state during the growth of $Ta_2O_5$ films on the Si substrate by ALD was investigated using in-situ synchrotron radiation photoemission spectroscopy. A newly synthesized liquid precursor $Ta(N^tBu)(dmamp)_2$ Me was used as the metal precursor, with Ar as a purging gas and $H_2O$ as the oxidant source. The core-level spectra of Si 2p, Ta 4f, and O 1s revealed that Ta suboxide and Si dioxide were formed at the initial stages of $Ta_2O_5$ growth. However, the Ta suboxide states almost disappeared as the ALD cycles progressed. Consequently, the $Ta^{5+}$ state, which corresponds with the stoichiometric $Ta_2O_5$, only appeared after 4.0 cycles. Additionally, tantalum silicide was not detected at the interfacial states between $Ta_2O_5$ and Si. The measured valence band offset value between $Ta_2O_5$ and the Si substrate was 3.08 eV after 2.5 cycles.

• 한국균학회지
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• 제26권4호통권87호
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• pp.562-573
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• 1998

간척연도가 다른 두 간척지의 고등식물의 근권에 존재하는 arbuscular mycorrhizal fungi(AMF)의 포자밀도, 공생강도와 토양의 물리 화학적 특성 및 AMF포자의 수직분포 등을 조사하여 토양특성에 따른 AMF의 공생생활성의 반응, 식물의 인 흡수에 미치는 AMF의 영향 등을 규명하였다. 간척지에서 AMF 포자의 수직분포는 포자가 표토로부터 20cm 깊이 이내에 분포하는데 그 원인은 뿌리의 수직분포, 지하수위 및 토양의 통기(aeration)와 관계가 있을 것이다. 토양 특성의 수직분포 중에서 토심이 깊어짐에 따라 증가되는 토양요인을 W.C., A-P 및 K이었고, 감소하는 요인은 O.M, Ca 및 Zn이었다. AMF의 공생생활성인 포자밀도와 공생강도는 토양의 pH, O.M., T-N 함량이 증가함에 따라 증가되고 W.C., A-P, Na 함량이 증가함에 따라 감소되는 두 반응 패턴이 나타났다. 간척지 식물의 인 흡수는 AMF의 공생활성이 커짐에 따라 증가되고, AMF의 공생활성은 토양의 인 함량이 적음에 따라 커짐으로써 식물은 AMF의 공생활성에 의하여 인 함량이 적은 토양에서 많은 양의 인을 흡수하였다.