• 한국수정란이식학회지
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• 제17권3호
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• pp.195-201
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• 2002

본 실험은 원시난포의 체외배양 체계를 확립할 수 있는 가능성을 검토하기 위해 0, 5, 10, 15 및 20일령 마우스에서 분리한 난소를 20, 15, 10, 5 및 0일동안 organ culture 하여 난소의 성장률, 난자의 회수율과 성장률 및 난자의 핵성숙 단계를 조사하여, 비교 ·검토하였다. 그 결과를 요약하면 다음과 같다. 1. Organ culture 전과 후의 각 일령에 따른 난소의 면적 차이는 0일령 35.9%, 5일령 8.7%, 10 일령 1.2% 및 15일령 14.4%로 15일령을 제외 하고는 배양일령이 증가할수록 면적 차이는 감소하였다. 2. Organ culture 후 난자의 회수율과 난자의 직경은 배양일령이 증가할수록 증대되었다. 3. GV기 이상의 핵성숙은 organ culture 후 배양 일령이 증가할수록 진행되는 단계에 있는 것으로 나타났다.

• Toxicological Research
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• 제16권4호
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• pp.275-284
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• 2000

The organ culture model of the whole mammary gland has many advantages for the study of branching morphogenesis and biological characteristics, including tumorigenesis. Prior to whole gland organ culture, rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA) or N-methyl-N-nitrosourea (MNU) for one week. The tramdorming effect and the morphological changes were assessed by the whole mount preparations and histopathological examination in terminal end buds (TEB), terminal ducts (TD), alveolar buds (AB), alveolar lobules (AL) and hyperplastic alveolar nodules (HAN) of the mammary gland. Grossfindings of the mammary glands at dissection were higher branching morphogenesis and larger volume in carcinogen-treated groups than in carcinogen-non-treated groups. Results of the whole mount method were coincided with those of the histopathological observations. Circular TEB, normally maintained AB, AL, and high cellular density were more frequently observed in carcinogen-treated groups than in carcinogen-nan-treated groups. Histopathologically, as a preneoplastic marker, HAN was maintained only in mammary organ culture of the carcinogen-treated groups. These findings suggest that in vivo trans-formation effects by carcinogens persisted during the mammary organ culture. These results were more characteristic in DMBA than in MNU-treated group. Ducts and terminal ducts appeared to have lost morphology during their growths in case of without diethylstilbestrol (DES). The fact that in vitro organ culture without DES was resulted in abnormal ductular morphogenesis confirms that DES is a physiological regulator of ductular epithelial cell growth.

• KSBB Journal
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• 제18권6호
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• pp.435-439
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• 2003

형질 전환된 담배 seed에서 담배 식물체를 유도하여 White 액체 배지에서 기관 배양하였다. 암조건, sucrose 2%의 조건에서 좋은 growth pattern을 나타내었고, 계대 배양은 외마디법을 이용하여 2주마다 하였다. 기관 배양에서 hGM-CSF production pattern을 보면, intracellular에서는 큰 변화 없이 약 30 ng/g의 일정한 농도를 나타내었다. Extracellular에서 hGM-CSF 농도는 배양 6일 이후부터 급속하게 증가하기 시작하여 배양 12일째에 약 0.2ng/$m\ell$의 농도를 나타낸다. 기관배양은 다른 식물세포 배양 시스템에 비해 생산되어진 단백질의 안정성이 크다는 장점에 비해 세포 내에서 배지 내로의 단백질 분비가 적다는 단점이 있다. 이를 극복하기 위해 다양한 permeabilizing agents를 투여하여 담배 세포의 permeability를 증가시키고자 하였다. 그 결과, Pluronic F-68과 PEG8000을 첨가한 경우 담배 세포에서 배지 내로의 단백질 분비가 원활해졌음을 확인할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.187-193
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• 2009

The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for long periods of time in culture. The colonies displayed morphology similar to miceSSC and were positive for markers of SSC (PGP9.5), proliferating germ cell (PigVASA), pre-meiotic germ cell (DAZL) and pluripotency (OCT4, SSEA-1, NANOG, and SOX2) based on immuno-cytochemistry and RT-PCR. The purity of these colonies was confirmed by negative expression of markers for sertoli cell (GATA4 and SOX9), peritubular myoid cell (${\alpha}$-SMA), differentiating spermatogonial and germ cells (c-KIT). The colonies could be maintained with undifferentiated morphology for more than two months and passaged more than 8 times with doubling time between 6-7 days. Taken together, we conclude that pigSSC could be successfully isolated and cultured in vitro and they possess characteristics similar to miceSSC.

• 대한치과교정학회지
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• 제19권1호
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• pp.61-75
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• 1989

Recently, indirect evidences suggest that Na-Ca exchange mechanism is involved in bone resorption. To study this suggestion, effects of several drugs which increase the intracellular sodium concentration by different mechanisms on the PTH-induced bone resorption were analysed employing organ culture. Ulnae and radii were removed from 19-day fetal rats, prelabelled by subcutaneous injection of $200{\mu}\;Ci^{45}CaC1_2$ on the 17th day of gestation, and then explanted on the membrane filters in organ culture dishes. For studying the effects of amiloride, ouabain, monensin, and veratridine on the PTH-induced bone resorption, control group was cultured in BGJb media containing PTH (0.4U/ml) while experimental group was cultured in BGJb media containing PTH and drugs. The effects of drugs on the PTH-induced bone resorption were observed by the ratios of $\%-release$ of $^{45}Ca$ between paired control and experimental groups. The results were as follows: 1. $^{45}Ca$ release was significantly increased by PTH (0.4U/ml) at 48 and 72 hours of culture. 2. Amiloride, at concentration of $500{\mu}M$, significantly inhibited the PTH-induced bone resorption after 48 and 72 hours of culture. 3. Ouabain, at concentration of 0.1 mM, presented significant inhibition of PTH-induced bone resorption after 48 and 72 hours of culture, and at 0.5mM and 1mM, presented significant inhibition of PTH-induced bone resorption after 72 hours of culture. 4. Monensin, at concentration of 500nM, significantly inhibited PTH-induced bone resorption after 72 hours of culture. 5. Veratridine, at concentration of 0.5mM, presented significant inhibition of PTH-induced bone resorption after 48 and 72 hours of culture, and at 1mM, presented significant inhibition of PTH-induced bone resorption after 72 hours of culture. Taken altogether, these results suggest that Na-Ca exchange mechanism play a role in PTH-induced bone resolution.

• Clinical and Experimental Pediatrics
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• 제52권8호
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• pp.856-861
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• 2009

Cardiopulmonary arrest has long been accepted as an unquestionable definition of death. An advent of cardiopulmonary resuscitation and artificial ventilation along with the development of organ transplantation has prompted the emergence of the concept of brain death. The criteria for brain death are based mainly on the clinical examination of coma, apnea and total loss of brain stem function. Although organ transplantation by donor brain death has increased in Korea over recent years, there is still a substantial shortage of donor organs compared to the demand. Improvement of government policies and changes of social culture for organ donation are needed for the activation of organ transplantation by donor brain death. Pediatricians have an important role for the search of potential donors in cases of brain death and optimal medical care for successful organ transplantation.

• Korean Chemical Engineering Research
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• 제44권1호
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• pp.58-64
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• 2006

본 연구에서는 인체 두피조직에서 미세수술법을 이용하여 모낭을 성공적으로 분리하였으며 다양한 조건으로 액침기관배양을 수행하였다. 우태아 혈청첨가시 모낭의 길이 성장이 저해되는 것으로 확인되어 무혈청 배지 조성을 시도하였다. 무혈청 배지로는 모낭 기관배양에 널리 이용되는 Williams'E medium을 기본으로 하는 Philpott medium과 자체 개발한 고농도 아미노산과 비타민(B군) 조성의 DHGM(Dongguk hair growth medium)을 이용하였다. 그리고 IMDM은 DHGM의 비교 대조군으로 이용하였다. 연구 결과 Philpott medium과 IMDM으로 배양한 모낭은 구조상으로는 길이 성장이 각각 9일과 12일 정도에 멈추며, 낮은 알카라인 포스파테이즈 발현, CK19 발현이 거의 없는 것으로 보아 세포사멸에 의한 퇴화(regression)가 빠르게 일어나는 것으로 판단되었다. 반면, DHGM으로 배양한 모낭은 상대적으로 긴 기간 동안 성장기의 구조를 보이며 25일까지 지속적인 길이 증가 및 3배 높은 알카라인 포스파테이즈 발현, 전반적인 CK19 발현을 나타내었다. 따라서 고농도의 아미노산 및 비타민 배지 조성이 생체 외에서 모낭을 장기간 배양하는데 중요한 역할을 하는 것으로 판단된다. 이러한 배양 방법은 장기간 검사를 필요로 하는 모낭에 대한 기초 생물학적 연구뿐만 아니라 새로운 탈모치료제의 효능 평가 분야에 이용될 수 있을 것이다.

• 문화기술의 융합
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• 제8권1호
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• pp.75-83
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• 2022

본 연구는 간호대학생의 생명의료윤리의식, 간호전문직관이 장기이식 태도에 미치는 영향을 파악하기 위해 시도되었다. 연구 대상자는 G, P시에 소재하고 있는 4년제 간호대학에 재학중인 간호대학생 202명으로 자료수집기간은 2021년 10월 12일부터 10월 22일까지였다. 자료는 SPSS 20.0 통계프로그램의 빈도분석, 평균과 표준편차, t-test와 One way ANOVA, Pearson's correlation coefficient, Stepwise multiple regression으로 분석하였다. 본 연구대상자의 생명의료윤리의식은 3.39±0.44, 간호전문직관은 3.84±0.56, 장기이식 태도는 3.67±0.25였다. 장기이식 태도에 영향을 미치는 요인으로 생명의료윤리의식의 신생아의 권리, 안락사영역, 간호전문직관의 사회적 인식, 간호의 전문성, 간호계의 역할영역이 장기이식 태도를 52.4% 설명하는 것으로 나타나 본 연구결과를 토대로 간호대학생의 장기이식 활성화를 위한 간호교육 자료로 활용 할 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.483-491
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• 2009

In vitro produced porcine embryos have potential application in reproductive biotechnology. However, their development potential has been very low. This study evaluated the in vitro developmental ability and quality of cloned and parthenogenetic porcine embryos having 2-4 cells or 5-8 cells on Day 2 of in vitro culture. Analysis of results showed that 2 to 4 cell embryos had higher ability to form blastocysts than 5 to 8 cell embryos (p<0.05). Blastocysts produced from culture of 2 to 4 cell embryos also contained higher cell numbers and had lower BAX:BCLxL transcript ratio than those produced from 5 to 8 cell embryos (p<0.05), thereby suggesting 2 to 4 cell embryos have higher development potential. Further investigation revealed that 5 to 8 cell embryos had higher incidence (100${\pm}$0.0%) of blastomeric fragmentation than 2 to 4 cell embryos (15.2${\pm}$5.5% for parthenogenetic and 27.7${\pm}$7.1% for cloned embryos). This suggests that low development potential of 5 to 8 cell embryos was associated with blastomeric fragmentation. In conclusion, we have shown that morphological selection of embryos based on cell number on Day 2 of in vitro culture could offer a practical and valuable non-invasive means to select good quality porcine embryos.

• Journal of Pharmaceutical Investigation
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• 제36권4호
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• pp.263-269
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• 2006

Deficiencies in the early ADMET(absorption, distribution, metabolism, elimination and toxicity) information on drug candidate extract a significant economic penalty on pharmaceutical firms. Microscale cell culture analogue-microscale gastrointestinal(${\mu}CCA-{\mu}GI$) device using Caco 2, L2 and HEp G2/C3A cells, which mimic metabolic process after absorption occurring in humans was used to investigate the toxicity of the model chemical, acetaminophen(AAP). The toxicity of acetaminophen determined after induction of CYP 1A1/2 in Caco 2 cells was not significant. In a coculture system, although no significant reduction in viability of HEp G2/C3A and L2 cells was found, approximately 5 fold increase in the CYP 1A1/2 activity was observed. These results appear to be related to organ-organ interaction. The oral administration of a drug requires addition of the absorption process through small intestine to the current ${\mu}CCA$ device. Therefore, a perfusion coculture system was employed for the evaluation of the absolution across the small intestine and resulting toxicity in the liver and lung. This system give comprehensive and physiologic information on oral uptake and resulting toxicity as in the body. The current ${\mu}CCA$ device can be used to demonstrate the toxic effect due to organ to organ interaction after oral administration,