• KSBB Journal
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• 제9권4호
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• pp.418-427
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• 1994

For the production of ${\alpha}$-amylase cloned from Bacillus licheniformis expressed in E. coli, cultivating factors including the concentrations of glucose, maltose and acetic acid were investigated. The results were as follows: 1) Maximum ${\alpha}$-amylase yield and maximum specific production rate obtained from glucose source were better than those achieved from maltose source. 2) The optimum production yield of ${\alpha}$-amylase was obtained at 1.0ml/$\ell$ or less of initial acetic acid concentration.

• KSBB Journal
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• 제13권3호
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• pp.284-288
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• 1998

Production of inulo-oligosaccharides from inulin was carried out with the maximum yield of 94% using a purified endoinulinase from Aspergillus ficcum. The optimum reaction temperature and pH were 55-60$^{\circ}C$ and pH 5.5-6.0, respectively. The Michaelis constant and maximum reaction velocity of the endoinuinase for inulin were 13.27 g/L and 0.13 g/L$.$min at 55$^{\circ}C$, pH 5.5, respectively. Inulin source had no significant effect on oligosaccharide yield and product composition, although initial production rate differed according to inulin origins. The reaction pH was a critical factor in inulo-oligosaccharide production because considerable free monosaccharides were released, decreasing oligosaccharide yield at low pH conditions. An empirical relationship describing the reaction performance was developed from kinetic data: the time to reach maximum oligosaccharide yield (tw) as a function of initial concentration of inulin (lo) and enzyme (Eo); i.e., log tM = -1.025 log Eo - 0.011 l0 + 2.655.

• 한국작물학회지
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• 제42권4호
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• pp.424-428
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• 1997

This study was carried out to investigate the effects of sowing dates, Apr. 25, May 15, June 5 and June 25, on sex ratio, growth and seed yield of "Chonnam species", local variety of hemp plant. The results were following as. : In sex ratio according to the different seeding dates, the revealating rate of female and male plants were highest on June 25, and Apr. 25, respectively. The length of stem, the number of branches, nodes, and seed yield were increased on May 15, but these charateristics were rapidly reduced on June 25 because of short growing period. Yield of seeds was increased by 24％ on May 15 comparing with 1.30ton /ha on Apr. 25.

• KSBB Journal
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• 제30권2호
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• pp.53-57
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• 2015

In this study, the production of total reducing sugar from macro green-algae Enteromorpha intestinalis by enzymatic hydrolysis was investigated. As a result of enzymatic hydrolysis using 13 kind commercial enzymes, the highest yield of 8.75% was obtained from Viscozyme L, which is multi-enzyme complex such as cellulase, arabanase, beta-glucanase, hemicellulase and xylanase. As a control, only 0.33% and 0.27% yield were obtained from 1% sulfuric acid and 0.05 M citrate buffer (pH 4.8), respectively. In the case of enzyme mixture, the mixture of $Viscozyme^{(R)}$ L and $Cellic^{(R)}$ CTec2 (1:1) was presented the highest yield of 10.67%. Finally, the 14.99% yield was obtained at 36 hr under the condition of 10% biomass and 30% enzyme mixture.

• Journal of Microbiology
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• 제34권3호
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• pp.255-262
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• 1996

The lipopolysaccharide (LPS) yields were measured in Rhodobacter capsulatus under several conditions by the ELISA method. The purification of LPS was done by affinity chromatography of IgG coupled CNBr-activated sepharose-4B instead of ultra-centrifugation. The purity of the LPS didn't show much difference between affinity chromatography and ultra-centrifugation method, but affinity chromatography method required much fewer organisms and was more convenient. LPS yield was measured in ng units by the ELISA method. Mannitol was a better single carbon source than other sugars, but mixing two carbon sources resulted in greater LPS yields than any sugar alone. LPS yield was directly proportional to $NH_ 4CI$ concentration, with optimum yields at 0.05% nitrogen. In contrest to LPS yields, which decreased at 0.005% nitrogen concentration total protein was increased 16 times. Calcium influenced LPS yields. At 0.7 mM $CaCI_ 2$, the LPS yield was 16.5 $\mu$g/mg DW, five times the yield without calcium.

• 한국자원식물학회지
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• 제17권2호
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• pp.102-106
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• 2004

남부 지방의 답전작 재배에 적합한 왕골의 재식밀도를 구명코자 다수성 품종으로 선발된 광산조생종을 공시하여 시험했다. 그 결과, 경장, 분얼수, 출수된 경수, 경태 등의 수량구성형 질은 18${\times}$18 cm 이식구에서 가장 우수하였고, 건피수량, 건수 수량에서도 18${\times}$18 cm 이식구에서 가장 우수하였다.

• 한국자원식물학회지
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• 제17권2호
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• pp.135-138
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• 2004

남부지방에서 답전작 재배로 적합한 이식기를 구명코자 다수성 품종으로 선발된 광산조생 재래종을 공시하여 시험을 수행하였다. 경장, 분얼수는 5월 10일과 5월 20일 이식구에서 가장 우수하였으며 출수된 경수와 경태(장경, 중경, 단경)은 5월 20일 이식구에서 가장 우수하였다. 또한 건피 수량(장경, 중경, 단경)과 건수수량은 5월 20일 이식구에서 가장 우수하였다.

• Plant Resources
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• 제8권1호
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• pp.67-70
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• 2005

To determine the optimal fertilizer level of wanggol in southeren areas of Korea, Gwangsan ealy local, the highest yielding variety was grown under nine different fertilezer levels. Yield components such as stem length, number of tillers, stem diameter were the highest at the treatments of fertilizer levels, $N-P_{2}O_{5}-K_{2}O=7-2-3$ and 9-2-3 kg/10a plots. Dry cortex and medulla yield were the highest at the treatments of $N-P_{2}O_{5}-K_{2}O=7-2-3$ and 9-2-3 kg/10a fertilizer levels. Judging from the results, an optimum fertilizer level of wanggol seemed to be $N-P_{2}O_{5}-K_{2}O=7-2-3$ and 9-2-3 kg/10a.

• 소성∙가공
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• 제10권1호
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• pp.53-58
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• 2001

Set-up reference values, used in determining the optimum downcoiling tension, we experimentally verified in this study. During the actual downcoiling, the strip suffers both tension and bending force through the rotation of mandrel. Therefore, simulative test which can measure both tension and bending resistance of strip was performed to estimate set-up reference value for strip tension during downcoiling operations. The values obtained from the simulative test were correlated with the yield stress which has conventionally been used as reference values for downcoiling tension. The correlative analysis showed that the yield stress of strip can be a good reference value for downcoiling tension. Furthermore, the bending load also shows strong correlation with simulated values due to the close relationship between yield stress and bending load.

• Archives of Pharmacal Research
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• 제25권4호
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• pp.453-456
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• 2002

Tonin(Persicae Semen) is the herb medicine that contains amygdalin as a major ingredient. Amygdalin in water is decomposed into benzaldehyde, HCN, and glucose by emulsin, a hydrolysis enzyme in tonin. A useful and practical method for the optimum extraction condition of amygdalin without enzymatic hydrolysis is required. The extraction yield of amygdalin of natural formula to nin was 0.1 % from crude powders, 1.4% from small pieces, 3.5% from half pieces and 2.4% from whole pieces. The extraction yield of amygdalin of outer shell-eliminated to nin was 0.3% from crude powders, 1.4% from small pieces, and 3.5% from half pieces and whole pieces respectively. The extraction yield of amygdalin was most high when using the size larger than half.