A Study on the Production of $\alpha$-amylase from Bacillus licheniformis Expressed in E. coli

대장균에서 발현된 B. licheniformis의 $\alpha$-amylase 생성에 관한 연구

For the production of ${\alpha}$-amylase cloned from Bacillus licheniformis expressed in E. coli, cultivating factors including the concentrations of glucose, maltose and acetic acid were investigated. The results were as follows: 1) Maximum ${\alpha}$-amylase yield and maximum specific production rate obtained from glucose source were better than those achieved from maltose source. 2) The optimum production yield of ${\alpha}$-amylase was obtained at 1.0ml/$\ell$ or less of initial acetic acid concentration.