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http://dx.doi.org/10.7841/ksbbj.2015.30.2.53

Optimum Reaction Condition of Enzymatic Hydrolysis for Production of Reducing Sugar from Enteromorpha intestinalis  

Kim, A-Ram (Department of Biotechnology, Pukyong National University)
Kim, Dong-Hyun (Department of Biotechnology, Pukyong National University)
Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University)
Publication Information
KSBB Journal / v.30, no.2, 2015 , pp. 53-57 More about this Journal
Abstract
In this study, the production of total reducing sugar from macro green-algae Enteromorpha intestinalis by enzymatic hydrolysis was investigated. As a result of enzymatic hydrolysis using 13 kind commercial enzymes, the highest yield of 8.75% was obtained from Viscozyme L, which is multi-enzyme complex such as cellulase, arabanase, beta-glucanase, hemicellulase and xylanase. As a control, only 0.33% and 0.27% yield were obtained from 1% sulfuric acid and 0.05 M citrate buffer (pH 4.8), respectively. In the case of enzyme mixture, the mixture of $Viscozyme^{(R)}$ L and $Cellic^{(R)}$ CTec2 (1:1) was presented the highest yield of 10.67%. Finally, the 14.99% yield was obtained at 36 hr under the condition of 10% biomass and 30% enzyme mixture.
Keywords
Enteromorpha intestinalis; Enzymatic hydrolysis; Reducing sugar;
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Times Cited By KSCI : 9  (Citation Analysis)
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