• Ocean Systems Engineering
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• v.2 no.3
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• pp.205-215
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• 2012

Recently, interest of offshore structure construction in South Korea is growing as the land space becomes limited for further development and the renewable energy grows to be more attractive for the replacement of the fossil energy. In order for the optimal construction of optimum offshore floating structures, development of safe and economical offshore foundation technologies is a priority. In this study, the large-deformation behavior of a suction pile, which markets are rapidly growing nowadays, is analyzed for three different loading locations (top, middle, and bottom of the suction pile) with three different displacement inclinations (displacement controlled with displacement inclinations of 0, 10, and 20 degrees from the horizontal). The behavior analysis includes quantifications of maximum resistances, translations, and rotation angles of the suction pile. The suction pile with its diameter of 10 m and height of 25 m is assumed to be embedded in clay, sand, and multi layers of subsea foundation. The soil properties of the clay, sand, and multi layers were determined based on the results of the site investigations performed in the West sea of South Korea. As analyses results, the maximum resistance was observed at the middle of the suction pile with the displacement inclination of 20 degrees, while the translations and rotations resulting from the horizontal and inclined pullouts were not significant until the horizontal components of movements at the loading points reach 1.0 m.

• Journal of Korea Water Resources Association
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• v.44 no.6
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• pp.439-447
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• 2011

This study aims to test the feasibility of combined use of EFDC (Environmental Fluid Dynamics Code) hydrodynamic model and WASP7.3 (Water Quality Analysis Program) model to improve accuracy of water quality predictions of the Yongdam Lake, Korea. The orthogonal curvilinear grid system was used for EFDC model to represent riverine shape of the study area. Relationship between volume, surface and elevation results were checked to verify if the grid system represents morphology of the lake properly. Monthly average boundary water quality conditions were estimated using the monthly monitored water quality data from Korean Ministry of Environment DB system. Monthly tributary flow rates were back-routed using dam discharge data and allocated in proportion to each basin area as direct measurements were not available. The optimum number of grid system was determined to be 372 horizontal cells and 10 vertical layers of the site for 1 year simulation of hydrodynamics and water quality out of iterative trials. Monthly observed BOD, TN, TP and Chl-a concentrations inside the lake were used for calibration of WASP7.3 model. This study shows that EFDC and WASP can be used in series successfully to improve accuracy in water quality modeling. However, it was observed that the amount of data to develop inflow water quality and flow rate boundary conditions and water quality data inside lake for calibration were not enough for accurate modeling. It is suggested that object-oriented data collection systems would be necessary to ensure accuracy of EFDC-WASP model application and thus for efficient lake water quality management strategy development.

• Journal of Life Science
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• v.21 no.9
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• pp.1323-1328
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• 2011

We herein report the development of an agarose-gel-immobilized recombinant bacterial biosensor simple system for the field monitoring of phenolic compounds. Escherichia coli cells harboring the pLZCapR plasmid, which was previously designed to express the ${\beta}$-galactosidase reporter gene in the presence of phenolic compounds, were co-immobilized with a substrate [chlorophenol red ${\beta}$-galactopyranoside (CPRG) in agarose gel, and dispensed to the wells of a 96-well plate. Field samples were added to the wells and color development was monitored. In the presence of 5 ${\mu}M$ to 10 mM of phenol, the biosensor developed a red (representing hydrolysis of CPRG) color. Other phenolic compounds were also detected by this immobilized system, with the pattern resembling that previously reported for the corresponding non-immobilized biosensor. The immobilized cells showed optimum activity when the gel was simultaneously supplemented with 6% dimethyl formamide (DMF), 0.1% SDS and 10 mM $CaCl_2$. The immobilized biosensor described herein does not require the addition of a substrate or the use of unwieldy instruments or sample pretreatments that could complicate field studies.

• Geotechnical Engineering
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• v.13 no.1
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• pp.59-74
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• 1997

This paper presents the results of the geotechnical investigation and settlement analysis of a finished waste landfill to find the possibility of the site as a construction area. Also, the variations of the strength of the municipal waste after mixing with the several types of the particulate grouts are investigated. The materials of the grouts used in the experiment are Quick Lime, Portland Cement, Slag Cement and Geocrete Cement. The results of the geotechnical investigation show that the maximum dry unit weight of the waste becomes lower and optimum moisture content higher as the age of the disposed waste is younger and the organic content is higher. The thickness of the predicted differential settlements of the waste fill has large difference from location to location and the unconfined compression strength of the grout mixed waste from the experiment was higher in the order of Geocrete Cement, Slag Cement, Portland Cement and Quick Lime.

• Journal of the Korea institute for structural maintenance and inspection
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• v.12 no.2
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• pp.149-155
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• 2008

The road construction, as part of effort to ease the worsening traffic, has been underway throughout the nation, while the existing road has been increasingly losing its load carrying capacity due to such factors as heavy traffic and weathering. In the case of site, the soil type, plasticity index, and specific gravity were SC, 12.2%, and 2.66, respectively. The maximum dry density, optimum moisture content and modified CBR were $1.895g/cm^3$ (Modified Compaction D), 13.6%, and 16.2%, respectively. A correlation of coefficient expressed good interrelationship by 0.90 between the CBR estimated from a dynamic penetration index of dynamic cone penetrometer test and a deformation modulus converted from a dynamic deflection modulus obtained from a portable FWD test.

• Journal of the Korean Recycled Construction Resources Institute
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• v.7 no.4
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• pp.358-366
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• 2019

The purpose of this study is to explore the possibility of estimating optimum surface finishing work time of the fresh concrete placed at the job site by applying a surface hardness test meter(Durometer). Tests are carried out by measuring and comparing the Proctor penetration resistance test and hardness test by Durometer. Correlations between Procter penetration test and hardness test by Durometer were obtained. Two different types Durometer were applied to estimate setting time. Test results indicate that the measurement of the Durometer and the test of the Proctor penetration resistance are highly correlated. When measuring the initial setting time with Durometer, initial setting time is reached when the hardness value by the type C Durometer is reached around 42HD, and when final setting is measured with the type D Durometer, the surface finishing work time limit and curing time can be estimated with 10HD of Durometer.

• Explosives and Blasting
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• v.32 no.3
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• pp.18-25
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• 2014

Ground vibration and noise from blasting operation are known to be the most representative constituents which can cause human and material damage. In this study, the effect of delay time on ground vibration is investigated by adopting seven different delay times in bench blasting. For each delay time, three blasting operations were performed. The prediction equations for blasting vibration are derived from 50 sets of measurement and the time theory of Langefors is evoked in the analysis of the blasting vibrations and frequencies. For the delay times of 8 ms and 28 ms, the average values of ground vibration are 5.76 cm/sec and 5.75 cm/sec, respectively, which are considerably low. Also the cyclic variation in the vibration measurements with the delay time confirms the interference effect. From the application of the measurements of blasting vibration and frequency to the time theory of Langefors, it is concluded that the optimum delay times are 8 ms and 24 ms for the test site.

• The Journal of Engineering Geology
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• v.1 no.1
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• pp.11-18
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• 1991

A series of laboratory tests were conducted to verify the unhardening phenomena of Soil-Cement Wall (SCW) and the results are presented in this paper. Specimens are prepared by mixing the site soil with cement and additives at a various ratio. The hydration of the cement mixed with the in-situ soil was retarded due to the higher organic content of the soil. In order to remove the influence of the organic matters in hydration reaction, calcium chloride (CaCI$_2$) was added as an acceleration additive at a different ratio. The optimum ratio of the calcium chloride for the higher SCW strength was determined as 2% of cement weight. The strength, however, was decreased by adding 4 and 6% of the additives. The effect of other additives, NaOH and NaSiO$_2$, were also investigated and the results are included. The strength of SCW by adding sodium hydroxide was lowered. And the short term strength by adding sodium silicate was increased but the long term strength was decreased.

• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1221-1226
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• 2008

The soft rot bacterium Pectobacterium wasabiae is an economically important pathogen of many crops. A new phytase gene, appA, was cloned from P. wasabiae by degenerate PCR and TAIL-PCR. The open reading frame of appA consisted of 1,302 bp encoding 433 amino acid residues, including 27 residues of a putative signal peptide. The mature protein had a molecular mass of 45 kDa and a theoretical pI of 5.5. The amino acid sequence contained the conserved active site residues RHGXRXP and HDTN of typical histidine acid phosphatases, and showed the highest identity of 48.5% to PhyM from Pseudomonas syringae. The gene fragment encoding the mature phytase was expressed in Escherichia coli BL21 (DE3), and the purified recombinant phytase had a specific activity of 1,072$\pm$47 U/mg for phytate substrate. The optimum pH and temperature for the purified phytase were pH 5.0 and 50$^{\circ}C$, respectively. The $K_m$ value was 0.17 mM, with a $V_{max}$ of 1,714 $\mu$mol/min/mg. This is the first report of the identification and isolation of phytase from Pectobacterium.

• Applied Biological Chemistry
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• v.37 no.5
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• pp.356-360
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• 1994

The optimum pH and temperature for endo-polygalacturonase activity from Jujube were 5.0 and $50^{\circ}C$. The range of its stability to pH was 4.0 to 5.0. The enzyme was inactivated about 35% by treatment at $70^{\circ}C$ for 1 hr. It was found that $Ag^+$, $Zn^{++}$ and $Mg^{++}$ increased the enzyme activity. In contrast, $Ba^{++}$, $Hg^{++}$, $Pb^{++}$, $Ca^{++}$, $Mn^{++}$, $Cu^{++}$, $Fe^{+++}$, $Na^+$ and $K^+$ decreased it. The enzyme was inactivated by treatment with maleic anhydride, iodine and 2,4-dinitrophenol. The results indicate that active site is a imidazole group on the enzyme.