• Title/Summary/Keyword: optimum medium

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Proliferation of Embryogenic Callus of Kalopanax pictus through Suspension Culture System (현탁배양을 통한 음나무(Kalopanax pictus) 배발생 캘러스의 증식)

  • Kim, Hye-Jin;Kim, Won-Bea;Yoo, Dong-Lim;Kim, Su-Jeong;Lee, Jun-Gu
    • Korean Journal of Plant Resources
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    • v.21 no.1
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    • pp.60-65
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    • 2008
  • Kalopanax pictus was cultured in vitro to find out optimal condition for embryogenic cells proliferation in liquid media rapidly. Embryogenic cells were induced from leaves and petiols of Kalopanax pictus. Optimum culture medium appeared to be a 1/2MS medium supplemented with 2.0mg/L 2,4-D and 0.1mg/L BA. To find out optimal conditions, embryogenic cells were cultured some condition as different concentrations of 2,4-D, medium and sucrose. There was cultured on 1/2MS liquid medium containing different concentration of 2,4-D. When embryogenic cells were cultured on 1/2MS liquid medium supplemented with 1.0mg/L 2,4-D, cell propagation rate was higher than other concentration of 2,4-D. When embryogenic cells were cultured on different media that MS, Gambols B5, N6, White, SH medium, observed the highest multiplication rate among Gambols B5 and White medium. To find out of effect of sucrose to embryogenic cells propagation, we tested cells under different concentrations. Optimal concentration of sucrose appeared to be a basal medium added 3% sucrose. Above results suggest that optimal conditions for proliferation of embryogenic cells were established Gambols B5 and White medium added 1.0mg/L 2,4-D and 3% sucrose. There is every possibility achieving embryogenic cells proliferation via bioreactor culture system in Kalopanax pictus.

Nondestructive Evaluation of Tempering Process of S45C Steel using DC Potential Drop Measurement (DC Potential Drop 측정을 이용한 S45C강의 템퍼링과정 비파괴 평가)

  • Park, Y.K.;Ahn, B.Y.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.9 no.1
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    • pp.77-82
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    • 1989
  • DC potential drop(DCPD) measurement system was made and the optimum measuring conditions have been studied. The room temperature resistivity of commercially available medium carbon steel(S45C) was measured using this technique. DCPD was measured in the specimens which were hardened and tempered at different temperatures. It was found that DCPD reflected the change of the microstructure during tempering very sensitively. It was possible to monitor the stage of tempering of S45C steel nondestructively using DCPD measurement.

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Enzymatic Glycosylation of Acrylic Acid and Methacrylic Acid (아크릴산과 메타크릴산의 효소적 배당화)

  • 박돈희;김해성
    • KSBB Journal
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    • v.16 no.1
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    • pp.82-86
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    • 2001
  • Glyco-acrylate and methacrylate were synthesized by lipase-catalyzed glycosylation of acrylic acid, methacrylic acid and their vinyl esters with $\beta$-methyl fructoside and glycerol in t-butanol as a reaction medium. At the optimum conditions for enzymatic glycosylation of acrylic acid and vinyl methacrylate, we attained up to 80% conversion for glyco-acrylate from acrylic acid and 90% conversion for glyco-methacrylate from vinyl methacrylate. The polymerizable glyco-acrylates and methacrylate have biomedical application as hydrophilic monomers and hydration modifiers to be use for hydrogel contact lens formulation.

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A Study of the Integrated Seakeeping Performance Index in Seaways (선박의 항해중 종합내항성능지표에 관한 연구)

  • 김순갑;공길영
    • Journal of the Korean Institute of Navigation
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    • v.21 no.2
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    • pp.1-9
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    • 1997
  • This paper presented a method of evaluating navigational safety of a ship by means fo the integrted seakeeping performance index(ISPI). The ISPI is defined by measuring only any one factor through the medium of relative dangerousness of the other factors for evaluating seakeeping performance. This evaluation method can be applied to the ships for any types and loading conditions. In devloping the practical evaluation system of navigational safety, it is expected to be useful to solve the difficulties in measuring factors by sensors. The results are also useful for devloping the optimum type of ship by applying at the initial design phase.

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광합성 홍색세균에 의한 5-Aminolevulinic acid생산에서의 조도의 영향

  • Min, Na-Yeong;Yun, Jong-Seon;Wi, Yeong-Jung;Kim, Jin-Nam;Ryu, Hwa-Won
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.295-296
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    • 2000
  • Effect of light intensity on ALA production was, investigated. The culture condition and medium optimization were also examined for the biosynthesis of ALA using Rhodobacter sphaeroides, non-sulfur bacteria, and investigated for enhancement of the production of ALA. In the dark condition, extracellular ALA formation and cell growth were not observed. Optimum light intensity for cell growth and ALA production were 4 kLux and 5 kLux, respectively.

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Nutritional Reguirements for Growth of Cellulomonas flavigena on cellulosic substrates (Cellulose기질에서 cellulomonas flavigena의 생장에 대한 영양요구성)

  • 한윤우
    • Korean Journal of Microbiology
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    • v.16 no.4
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    • pp.155-160
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    • 1978
  • Nutritional requirements for the growth of Cellulomonas flavigena were studied. C. flavigena grew well on cellulose when 0.005% or more of yeast extract was present in the growth medium. The growth factor in yeast extrct was, in part, thiamine and biotin. Amino acids had little effect on the growth on the organism. The extent of growth on yeast extract was much higher than that obtained on those vitamins, which indicates the presence of growth factors in yeast extract besides the vitamins, among the carbohydrates tested, the organism grew best on glucose and galactose, and the optimum N/P ratio was within the range of 0.75~3.17.

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Optimum Growth Condition of Phase Change GeSbTe Thin Films as an Optical Recording Medium using in situ Ellipsometry (In situ 타원법을 사용한 광기록매체용 GeSbTe 박막의 최적성장조건 연구)

  • 이학철;김상열
    • Proceedings of the Optical Society of Korea Conference
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    • 2003.02a
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    • pp.78-79
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    • 2003
  • 타원법(ellipsometry)을 사용하여 광기록 매체용 Ge$_2$Sb$_2$Te/sub 5/(GST) 박막의 성장과정에 따른 타원상수 Ψ와 $\Delta$를 측정하여, GST 박막의 최적성장조건을 연구하였다. 아르곤기체압력과 DC 출력 그리고 기판의 온도를 변화시키면서 GST 박막을 성장시켰다. 제작된 시료들의 분광타원 데이터를 모델링분석하여 GST 박막의 밀도분포를 구하고 한편으로는 GST 박막이 성장하는 동안 측정한 in situ 타원 성장곡선을 분석하여 박막의 밀도분포의 변화를 추적하였다. (중략)

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Optical IIR lattice fiber filter design for optimum of optical signal energy (광신호 에너지 최적화를 위한 IIR 격자형 광파이버필터 설계)

  • 이채욱;김신환
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.32B no.11
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    • pp.1481-1488
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    • 1995
  • Due to the low loss, broadband and accurate short time delay properties of optical fiber, it has attracted as a delay medium for high speed and broad-band signal processing. In this paper, we consider the coherent optical fiber filter of IIR lattice structure, which uses coherent light sources and consists of directional couplers whose coupling coefficients are restricted between 0 and 1. Considering restrictions of directional coupler, the design formulae and condition for realibility of optical fiber filter of IIR lattice structure which makes the optimal use of optical signal energy are derived.

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A Study of the Integrated Seakeeping Performance Index in Seaways (선박의 항해중 종합내항성능지표에 관한 연구)

  • 김순갑;공길영
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 1997.04a
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    • pp.5-16
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    • 1997
  • This paper presented a method of evaluting navigational safety of a ship by means of the integrated seakeeping performance index(ISPI). The ISPI is defined by measuring only any one factor through the medium of relative dangerousness of the other factors for evaluating seakeeping performance. This evaluation method can be applied to the ships for any types and loading conditions. In developing the practical evaluation system of navigational safety. it is expected to be useful for developing the optimum type of ship by applying at the initial design phase.

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Carbonylation of Benzal Chlorides to Alkyl Phenylacetates using $Fe(CO)_5$

  • Shim, Sang-Chul;Doh, Chil-Hoon;Lee, Sung-Yub;Cho, Chan-Sik
    • Bulletin of the Korean Chemical Society
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    • v.11 no.5
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    • pp.420-422
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    • 1990
  • Various substituted benzal chlorides are converted into alkyl phenylacetates ($ArCH_2COOR$) under CO atmosphere on reaction with a catalytic amount of Fe(CO)$_5$, a base, and an alcohol. The optimum conditions are found as atmospheric pressure of CO, 40$^{\circ}C$, potassium hydroxide as base, and medium of alcohol.