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Proliferation of Embryogenic Callus of Kalopanax pictus through Suspension Culture System  

Kim, Hye-Jin (National Institute of Highland Agriculture, RDA)
Kim, Won-Bea (National Institute of Highland Agriculture, RDA)
Yoo, Dong-Lim (National Institute of Highland Agriculture, RDA)
Kim, Su-Jeong (National Institute of Highland Agriculture, RDA)
Lee, Jun-Gu (National Institute of Highland Agriculture, RDA)
Publication Information
Korean Journal of Plant Resources / v.21, no.1, 2008 , pp. 60-65 More about this Journal
Abstract
Kalopanax pictus was cultured in vitro to find out optimal condition for embryogenic cells proliferation in liquid media rapidly. Embryogenic cells were induced from leaves and petiols of Kalopanax pictus. Optimum culture medium appeared to be a 1/2MS medium supplemented with 2.0mg/L 2,4-D and 0.1mg/L BA. To find out optimal conditions, embryogenic cells were cultured some condition as different concentrations of 2,4-D, medium and sucrose. There was cultured on 1/2MS liquid medium containing different concentration of 2,4-D. When embryogenic cells were cultured on 1/2MS liquid medium supplemented with 1.0mg/L 2,4-D, cell propagation rate was higher than other concentration of 2,4-D. When embryogenic cells were cultured on different media that MS, Gambols B5, N6, White, SH medium, observed the highest multiplication rate among Gambols B5 and White medium. To find out of effect of sucrose to embryogenic cells propagation, we tested cells under different concentrations. Optimal concentration of sucrose appeared to be a basal medium added 3% sucrose. Above results suggest that optimal conditions for proliferation of embryogenic cells were established Gambols B5 and White medium added 1.0mg/L 2,4-D and 3% sucrose. There is every possibility achieving embryogenic cells proliferation via bioreactor culture system in Kalopanax pictus.
Keywords
Liquid culture; Medium strength; Callus growth;
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