• Biotechnology and Bioprocess Engineering:BBE
• /
• 제7권4호
• /
• pp.201-205
• /
• 2002

The growth conditions of Pantoea aggicmerans, a phosphate solubilizing organism, were studied In our laboratory to determine the optimal conditions. Pantoea aggionerans showed the highest growth rate at 30$\^{C}$, pH 7.0 and 2 vvm, after 50 h cultivation. A certain relationship between pH and phosphate concentration was evident when the glucose concentration in the me dium was changed. Increasing glucose concentration increased the pH buffer action of the broth. At glucose concentrations higher than the optimum concentration of 0.2 M, the cell growth was retarded. P. agglomerans consumed glucose as a substrate to produce organic acids which caused the pH decrease in the culture medium. The phosphate concentration in the medium was increased by the presence of the organic acids, which solubilized insoluble phosphates such as hydroxyapa-tite.

• 한국식품영양학회지
• /
• 제8권3호
• /
• pp.253-261
• /
• 1995

A strain of alkalophilic Bacillus sp. C-21 has been Isolated from sold. The strain was capable of producing large amount of cyclodextrin glycosyltransferase (CGTase) in the high alkaline pH medium. The preferable medium composition was determined to be as follows : 1.0% soluble starch, 1.0% peptone, 0.5% yeast extract, 0.1% K2HP04, 0.02% MgSO4.7H2O and 1.0% Na2CO3(pH 10.0) The highest enzyme production was observed after 30hours of cultivation at 33$^{\circ}C$. The optimum temperature and pH for the activity of crude enzyme were 6$0^{\circ}C$ and 6.0, respectively. The enzyme was stable between pH 6.0 and 9.6, and up to 55$^{\circ}C$.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2006년도 추계학술대회논문집
• /
• pp.840-843
• /
• 2006

The determination of firing order is very significant procedure in initial stage of design for medium speed diesel engine. Generally, the selection of firing order has been accomplished in view of minimum excitation forces condition. In this paper all possible firing orders under the given number of cylinder were considered to decide the optimum. Meanwhile torsional vibration characteristics using the phase vector sum method and minimum excitation force concept were applied. From these results, some superior cases were selected. And then, the torsional vibration response analysis and the resonance characteristics of engine structure were investigated for the final decision.

• 한국신재생에너지학회:학술대회논문집
• /
• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
• /
• pp.251-254
• /
• 2009

Thermal performance of a thermosiphon for medium-temperature solar thermal application was investigated. The working fluid was Dowtherm A and the container was made of STS 316L. The thermosiphon had a outer diameter of 12.7 mm and a total length of 2 m, where the evaporator and the condenser had the same length of 0.3 m and the adiabatic section was 1.4 m. Both the evaporator and the condenser were aligned horizontal with an elevation difference of 0.18 m to utilize the gravitational force for the working-fluid return. The optimum fill charge ratio of the working fluid was investigated to obtain the maximum heat transport with the lowest thermal resistance. The maximun input thermal load was 500 W and thermal resistance was $0.60^{\circ}C/W$.

• Journal of Microbiology and Biotechnology
• /
• 제7권4호
• /
• pp.278-281
• /
• 1997

Streptomyces sp. MCY-846 selected by in vitro cytotoxicity assay produced elaiophylin. Individual characteristics of the strains such as spore morphology, and physiological characteristics indicated that the strain is resembled to Streptomyces hygroscopicus. The time course of cell growth and antibiotic production was observed in the medium containing 0.5% trehalose and 0.5% soybean meal as carbon and nitrogen sources, respectively. The optimum production of elaiophylin was tested with different combinations of carbon and nitrogen sources and reached a maxima of $470{\mu}$/ml in the PC-II medium.

• Journal of Microbiology and Biotechnology
• /
• 제24권2호
• /
• pp.188-196
• /
• 2014

Extracellular production of recombinant human bone morphogenetic protein-7 (rhBMP-7) was carried out through the fermentation of Bacillus subtilis. Three significant fermentation conditions and medium components were selected and optimized to enhance the rhBMP-7 production by using the response surface methodology (RSM). The optimum values of the three variables for the maximum extracellular production of rhBMP-7 were found to be 2.93 g/l starch, 5.18 g/l lactose, and a fermentation time of 34.57 h. The statistical optimization model was validated with a few fermentations of B. subtilis in shake flasks under optimized and unoptimized conditions. A 3-L jar fermenter using the shake-flask optimized conditions resulted in a higher production (413 pg/ml of culture medium) of rhBMP-7 than in a shake flask (289.1 pg/ml), which could be attributed to the pH being controlled at 6.0 and constant agitation of 400 rpm with aeration of 1 vvm.

• 한국식생활문화학회지
• /
• 제3권3호
• /
• pp.293-299
• /
• 1988

34 packaged meal (Dosirak) manufacturing establishments were assessed in terms of working environment, production and transportation practices. Questionnaires and facility check-lists were developed. Most establishments were small in business, and production personnel as well as production facilities were insufficient compared with production capacity of establishments. Mean production capacity for packaged meals in terms of optimum and maximum levels were 6,500 and 15,166 meals in large sized establishments; 2,662 and 8,301 in medium; and 2,112, and 4,733 in small respectively. Those figures indicate potentially hazardous practices in production especially in small and medium sized establishments. Most meals were produced to order. Transportation facility and kitchen space were assessed as insufficient.

• 한국미생물·생명공학회지
• /
• 제25권5호
• /
• pp.435-441
• /
• 1997

Forty seven strains of yeast were isolated from traditional Meju and were identified as Saccharomyces spp. (7 strains), Zygosaccharomyces spp. (7 strains), Kluyveromyces spp. and Hansenula spp. (each 5 strains), Rhodotorular spp. (8 strains), Candida spp. (12 strains), Pichia spp. and Debaryomyces spp. from results of their microbiological characteristics. The optimal medium for growth of all the yeasts was YM media and the optimal initial pH of the medium was 6.0. The optimum temperature for growth was 30$circ$ and among them, Sacch. exiguus OE-5, Sacch. cerevisiae OE-16, Sacch. kluyveri C-1 strains were thermotolerant yeasts which could grow at 40$circ$C.

• 한국미생물·생명공학회지
• /
• 제23권2호
• /
• pp.214-219
• /
• 1995

In fermentation of dairy products, bifidobacteria is used in conjunction with other lactic acid bacteria, such as L. acidophilus, L. bulgaricus and S. thermophilus, rendering the enumeration of bifidobacteria difficult. In order to develop optimum conditions for selective enumeration of bifidobacteria, we examined MIC of several antibiotics against various bifidobacteria and other lactic acid bacteria. The growth of L. acidophilus, L. bulgaricus and S. thermophilus were inhibited by lithium chloride at the concentration of less than 4 mg/ml, whereas growth inhibition of bifidobacteria occurred at concentrations over 6-10 mg/ml. Tetracycline and chloramphenicol were also found to selectively inhibit growth of other lactic acid bacteria at the concentration of 1-3 $\mu$g/ml. Addition of 6 mg/ml lithium chloride, 1 $\mu$g/ml, tetracycline or 3 $\mu$g/ml chloramphenicol to medium was found to be optimal for selective enumeration of bifidobacteria. By using these three inhibitory chemicals in the TPY medium, higher number of bifidobacteria were selectively isolated than with NPNL agar and LP agar.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVII)
• /
• pp.465-467
• /
• 2005

UIW-10 mutant obtained by UV treatment using sulfur-oxidizing bacteria, Thiobacillus sp. IW was studied. The colony size of UIW-10 was found 2 $^{\sim}$ 3 times bigger in diameter than the parent colony on TAM medium. UIW-10 mutant growth was two times higher than parent strain at 6 h culture in liquid medium containing sulfides such as sulfur and sodium thiosulfate. Initial pH and temperature for the optimum growth of UIW-10 were 6.0 and $35-40^{\circ}C$, respectively. It was found that addition of 0.5% yeast extract and 0.5 to 2.0% tryptone as nitrogen sources and the constant agitation at 150 to 200 rpm had a positive effect and the growth of UIW-10 was increased.