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http://dx.doi.org/10.4014/jmb.1308.08005

Optimization of Extracellular Production of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) with Bacillus subtilis  

Kim, Chun-Kwang (School of Applied Chemical Engineering, Chonnam National University)
Rhee, Jong Il (School of Applied Chemical Engineering, Chonnam National University)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.2, 2014 , pp. 188-196 More about this Journal
Abstract
Extracellular production of recombinant human bone morphogenetic protein-7 (rhBMP-7) was carried out through the fermentation of Bacillus subtilis. Three significant fermentation conditions and medium components were selected and optimized to enhance the rhBMP-7 production by using the response surface methodology (RSM). The optimum values of the three variables for the maximum extracellular production of rhBMP-7 were found to be 2.93 g/l starch, 5.18 g/l lactose, and a fermentation time of 34.57 h. The statistical optimization model was validated with a few fermentations of B. subtilis in shake flasks under optimized and unoptimized conditions. A 3-L jar fermenter using the shake-flask optimized conditions resulted in a higher production (413 pg/ml of culture medium) of rhBMP-7 than in a shake flask (289.1 pg/ml), which could be attributed to the pH being controlled at 6.0 and constant agitation of 400 rpm with aeration of 1 vvm.
Keywords
Bacillus subtilis; optimization; response surface methodology; rhBMP-7;
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