• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.1
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• pp.26-33
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• 1989

This pot experiments were conducted to find out the optimal fertilization ratios of the N:S:P anions and K:Ca:Mg cations of major nutrients in an orchardgrass/ladino clover mixed sward. The optimum ratios in equivalent basis were computed by the Homes systematic variations technique. The soil samples were collected from the newly reclaimed soils, which are located on colluvial hilly area with a good consideration for the hilly pasture development. The results were summarized as follows; 1, the optimum fertilization ratios of major nutrients for the high yields by the forage species in a mixed sward were obtained (Table 4 in detail); N:S:P = 3:l: 1 and K:Ca:Mg = 1:l: 1 for grass and grass plus legume, and N:S:P = 1:6:43 and K:Ca:Mg = 1: 3: 1 for legume in general. 2. The yield increases from the systematic variations in this mixed sward were laid in following order; N > P >S-group and K >Mg >Ca-group for grass and grass plus legume, and P >S >N-group and Ca >Mg > K-group for legume. Especially, the yields were greatly increased at the N-group for grass, and at the Pand Ca-groups for legume. 3. Soil pH-value was descreased at the Sgroup, and somewhat increased at the Ca- and Mg-groups. The content of available $P_2O_5$, CEC and base saturation were greatest with the Ca-group. 4. At the N-group, the N-contents were highest and the P-contents were lowest in grass and grass plus legume, which resulted in the highest Ca/P ratio of 2.15 among the anion groups. Whereas the highest Ca/P ratio of 9.20 in legume was obtained at the Ca-group. Legume showed in general higher Ca/P ratio and lower K/(Ca+Mg) ratio than these in grass. 5 . There were differences in the effects of systematic variations of major nutrients on the dry matter yields and the mineral yields. The optimum fertilization ratios of anions and cations for the high mineral yields were obtained (Table E), which showed differences comparing with the ratios for the high dry matter yields. The antagonis between the cations K and Mg was known from the point of mineral yields of mixed forages.

• Journal of Bio-Environment Control
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• v.29 no.2
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• pp.161-170
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• 2020

There is systematic spatial variations in environmental properties due to sensitive reaction to external conditions at plastic greenhouse occupied 99.2% of domestic agricultural facilities. In order to construct 3 dimensional distribution of temperature, relative humidity, CO₂ and illuminance, measurement matrix as 3 by 3 by 5 in direction of width, height and length, respectively, dividing indoor space of greenhouse was designed and tested at experimental site. Linear regression analysis was conducted to evaluate optimal estimation method in terms with horizontal and vertical variations. Even though sole measurement point for temperature and relative humidity could be feasible to assess indoor condition, multiple measurement matrix is inevitably required to improve spatial precision at certain time domain such as period of sunrise and sunset. In case with CO₂, multiple measurement matrix could not successfully improve the spatial predictability during a whole experimental period. In case with illuminance, prediction performance was getting smaller after a time period of sunrise due to systematic interference such as indoor structure. Thus, multiple sensing methodology was proposed in direction of length at higher height than growing bed, which could compensate estimation error in spatial domain. Appropriate measurement matrix could be constructed considering the transition of stability in indoor environmental properties due to external variations. As a result, optimal measurement matrix should be carefully designed considering flexibility of construction relevant with the type of property, indoor structure, the purpose of crop and the period of growth. For an instance, partial cooling and heating system to save a consumption of energy supplement could be successfully accomplished by the deployment of multiple measurement matrix.

• Korean Journal of Remote Sensing
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• v.39 no.5_1
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• pp.715-727
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• 2023

To provide detailed and appropriate meteorological information in mountainous areas, the Korea Forest Service has established an Automatic Mountain Meteorology Observation Station (AMOS) network in major mountainous regions since 2012, and 464 stations are currently operated. In this study, we proposed an optimal kriging technique with lapse rate correction to produce gridded temperature data suitable for Korean forests using AMOS point observations. First, the outliers of the AMOS temperature data were removed through statistical processing. Then, an optimized theoretical variogram, which best approximates the empirical variogram, was derived to perform the optimal kriging with lapse rate correction. A 500-meter resolution Kriging map for temperature was created to reflect the elevation variations in Korean mountainous terrain. A blind evaluation of the method using a spatially unbiased validation sample showed a correlation coefficient of 0.899 to 0.953 and an error of 0.933 to 1.230℃, indicating a slight accuracy improvement compared to regular kriging without lapse rate correction. However, the critical advantage of the proposed method is that it can appropriately represent the complex terrain of Korean forests, such as local variations in mountainous areas and coastal forests in Gangwon province and topographical differences in Jirisan and Naejangsan and their surrounding forests.

• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.56-62
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• 2011

We investigated quality characteristics of Seoktanju (one of the Korean traditional rice wine) which was fermented using five kinds of Korean commercial Nuruks. The purpose of this study was to research what effects on the quality of Seoktanju by using different Nuruks. We analyzed general component such as each mash's temperature change patterns, pH, titrable acidities, reducing sugar contents, volatile acids, and sugar contents during fermentation periods and studied sensory evaluation of produced Seoktanju (10 days). On the whole, temperature change patterns in the each mashes were depend on room temperature. All Seoktanju's pH was reduced rapidly up to three days after first mashing (pH 3.13-3.57) and after that was increased gradually. The end of fermentation pH was pH 3.6-4.05. Mostly, acidities were indicated high(0.59%) and Nuruk-B was showed highest acid value. These results seems to be different as occasion organic acids producing activity depend on the number of yeast, material contents, optimal temperature in the each mashes by fungi and lactic acid bacteria in Nuruks. In reducing sugar contents and sugar contents, Nuruk-C treatment were showed the highest value with 5.36%, $23^{\circ}brix$, respectively and alcohol content was lowest with 8.6%. In the five kinds of reproduced Seoktanju, alcohol content was the highest in the treated Nuruk-A group. Volatile acid value was the highest with 132.6~263.7 ppm at the 3 day after first mashing day but as the fermentation time goes on, it was reduced sharply by 5.25~5.94 ppm. Sensory evaluation was performed with 5 point scale, the Seoktanju using Nuruk-D was presented by 4 point, while Nuruk-A was presented lowest by 2.77 point on overall acceptability.

• Journal of Life Science
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• v.30 no.3
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• pp.313-319
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• 2020

Recently, cattle epidemic diseases are caused by a pathogen such as a virus or bacterium. Such diseases can spread through various pathways, such as feed intake, respiration, and contact between livestock. Diagnosis based on the ELISA (Enzyme-linked immunosorbent assay) and PCR (Polymerase chain reaction) methods has limitations because these traditional diagnostic methods are time consuming assays that require multiple steps and dedicated equipment. In this review, we propose the use of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Cas system based on DNA and RNA levels for early point-of-care diagnosis in cattle. In the CRISPR/Cas system, Cas effectors are classified into two classes and six subtypes. The Cas effectors included in class 2 are typically Cas9 in type II, Cas12 in type V (Cas12a and Cas12b) and Cas13 in type VI (Cas13a and Cas13b). The CRISPR/Cas system uses reporter molecules that are attached to the ssDNA strands. When the Cas enzyme cuts the ssDNA, these reporters either fluoresce or change color, indicating the presence of a specific disease marker. There are several steps in the development of a CRISPR/Cas system. The first is to select the Cas enzyme depending on DNA or RNA from pathogens (viruses or bacteria). Based on that, the next step is to integrate the optimal amplification, transducing method, and signal reporter. The CRISPR/Cas system is a powerful diagnostic tool using a gene-editing method, which is faster, better, and cheaper than traditional methods. This system could be used for early diagnosis of epidemic cattle diseases and help to control their spread.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.31 no.1
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• pp.28-39
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• 2019

In the design process of counter measures against the beach erosion, information like the main sediment transport mode and yearly net amount of longshore and cross shore transport is of great engineering value. In this rationale, we numerically analyzed the yearly sediment budget of the Mang-Bang beach which is suffering from erosion problem. For the case of cross sediment transport, Bailard's model (1981) having its roots on the Bagnold's energy model (1963) is utilized. In doing so, longshore sediment transport rate is estimated based on the assumption that longshore transport rate is determined by the available wave energy influx toward the beach. Velocity moments required for the application of Bailard's model (1981) is deduced from numerical simulation of the nonlinear shoaling process over the Mang-Bang beach of the 71 wave conditions carefully chosen from the wave records. As a wave driver, we used the consistent frequency Boussinesq Eq. by Frelich and Guza (1984). Numerical results show that contrary to the Bailard's study (1981), Irribaren NO. has non negligible influence on the velocity moments. We also proceeds to numerically simulate the yearly sediment budget of Mang-Bang beach. Numerical results show that for ${\beta}=41.6^{\circ}$, the mean orientation of Mang-Bang beach, north-westwardly moving longshore sediment is prevailing over the south-eastwardly moving sediment, the yearly amount of which is simulated to reach its maxima at $125,000m^3/m$. And the null pint where north-westwardly moving longshore sediment is balanced by the south-eastwardly moving longshore sediment is located at ${\beta}=47^{\circ}$. For the case of cross shore sediment, the sediment is gradually moving toward the shore from the April to mid October, whereas these trends are reversed by sporadically occurring energetic wind waves at the end of October and March. We also complete the littoral drift rose of the Mang-Bang beach, which shows that even though the shore line is temporarily retreated, and as a result, the orientation of Mang-Bang beach is larger than the orientation of null pont, south-eastwardly moving longshore sediment is prevailing. In a case that the orientation of Mang-Bang beach is smaller than the orientation of null pont, north-westwardly moving longshore sediment is prevailing. And these trend imply that the Mang-Bang beach is stable one, which has the self restoring capability once exposed to erosion.

• Journal of Intelligence and Information Systems
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• v.25 no.2
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• pp.39-55
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• 2019

Recently banks and large financial institutions have introduced lots of Robo-Advisor products. Robo-Advisor is a Robot to produce the optimal asset allocation portfolio for investors by using the financial engineering algorithms without any human intervention. Since the first introduction in Wall Street in 2008, the market size has grown to 60 billion dollars and is expected to expand to 2,000 billion dollars by 2020. Since Robo-Advisor algorithms suggest asset allocation output to investors, mathematical or statistical asset allocation strategies are applied. Mean variance optimization model developed by Markowitz is the typical asset allocation model. The model is a simple but quite intuitive portfolio strategy. For example, assets are allocated in order to minimize the risk on the portfolio while maximizing the expected return on the portfolio using optimization techniques. Despite its theoretical background, both academics and practitioners find that the standard mean variance optimization portfolio is very sensitive to the expected returns calculated by past price data. Corner solutions are often found to be allocated only to a few assets. The Black-Litterman Optimization model overcomes these problems by choosing a neutral Capital Asset Pricing Model equilibrium point. Implied equilibrium returns of each asset are derived from equilibrium market portfolio through reverse optimization. The Black-Litterman model uses a Bayesian approach to combine the subjective views on the price forecast of one or more assets with implied equilibrium returns, resulting a new estimates of risk and expected returns. These new estimates can produce optimal portfolio by the well-known Markowitz mean-variance optimization algorithm. If the investor does not have any views on his asset classes, the Black-Litterman optimization model produce the same portfolio as the market portfolio. What if the subjective views are incorrect? A survey on reports of stocks performance recommended by securities analysts show very poor results. Therefore the incorrect views combined with implied equilibrium returns may produce very poor portfolio output to the Black-Litterman model users. This paper suggests an objective investor views model based on Support Vector Machines(SVM), which have showed good performance results in stock price forecasting. SVM is a discriminative classifier defined by a separating hyper plane. The linear, radial basis and polynomial kernel functions are used to learn the hyper planes. Input variables for the SVM are returns, standard deviations, Stochastics %K and price parity degree for each asset class. SVM output returns expected stock price movements and their probabilities, which are used as input variables in the intelligent views model. The stock price movements are categorized by three phases; down, neutral and up. The expected stock returns make P matrix and their probability results are used in Q matrix. Implied equilibrium returns vector is combined with the intelligent views matrix, resulting the Black-Litterman optimal portfolio. For comparisons, Markowitz mean-variance optimization model and risk parity model are used. The value weighted market portfolio and equal weighted market portfolio are used as benchmark indexes. We collect the 8 KOSPI 200 sector indexes from January 2008 to December 2018 including 132 monthly index values. Training period is from 2008 to 2015 and testing period is from 2016 to 2018. Our suggested intelligent view model combined with implied equilibrium returns produced the optimal Black-Litterman portfolio. The out of sample period portfolio showed better performance compared with the well-known Markowitz mean-variance optimization portfolio, risk parity portfolio and market portfolio. The total return from 3 year-period Black-Litterman portfolio records 6.4%, which is the highest value. The maximum draw down is -20.8%, which is also the lowest value. Sharpe Ratio shows the highest value, 0.17. It measures the return to risk ratio. Overall, our suggested view model shows the possibility of replacing subjective analysts's views with objective view model for practitioners to apply the Robo-Advisor asset allocation algorithms in the real trading fields.

• Radiation Oncology Journal
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• v.23 no.3
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• pp.143-156
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• 2005

Background: The best dose-fractionation regimen of the definitive radiotherapy for cervix cancer remains to be clearly determined. It seems to be partially attributed to the complexity of the affecting factors and the lack of detailed information on external and intra-cavitary fractionation. To find optimal practice guidelines, our experiences of the combination of external beam radiotherapy (EBRT) and high-dose-rate intracavitary brachytherapy (HDR-ICBT) were reviewed with detailed information of the various treatment parameters obtained from a large cohort of women treated homogeneously at a single institute. Materials and Methods: The subjects were 743 cervical cancer patients (Stage IB 198, IIA 77, IIB 364, IIIA 7, IIIB 89 and IVA 8) treated by radiotherapy alone, between 1990 and 1996. A total external beam radiotherapy (EBRT) dose of $23.4\~59.4$ Gy (Median 45.0) was delivered to the whole pelvis. High-dose-rate intracavitary brachytherapy (HDR-IBT) was also peformed using various fractionation schemes. A Midline block (MLB) was initiated after the delivery of $14.4\~43.2$ Gy (Median 36.0) of EBRT in 495 patients, while In the other 248 patients EBRT could not be used due to slow tumor regression or the huge initial bulk of tumor. The point A, actual bladder & rectal doses were individually assessed in all patients. The biologically effective dose (BED) to the tumor ($\alpha/\beta$=10) and late-responding tissues ($\alpha/\beta$=3) for both EBRT and HDR-ICBT were calculated. The total BED values to point A, the actual bladder and rectal reference points were the summation of the EBRT and HDR-ICBT. In addition to all the details on dose-fractionation, the other factors (i.e. the overall treatment time, physicians preference) that can affect the schedule of the definitive radiotherapy were also thoroughly analyzed. The association between MD-BED $Gy_3$ and the risk of complication was assessed using serial multiple logistic regression models. The associations between R-BED $Gy_3$ and rectal complications and between V-BED $Gy_3$ and bladder complications were assessed using multiple logistic regression models after adjustment for age, stage, tumor size and treatment duration. Serial Coxs proportional hazard regression models were used to estimate the relative risks of recurrence due to MD-BED $Gy_{10}$, and the treatment duration. Results: The overall complication rate for RTOG Grades $1\~4$ toxicities was $33.1\%$. The 5-year actuarial pelvic control rate for ail 743 patients was $83\%$. The midline cumulative BED dose, which is the sum of external midline BED and HDR-ICBT point A BED, ranged from 62.0 to 121.9 $Gy_{10}$ (median 93.0) for tumors and from 93.6 to 187.3 $Gy_3$ (median 137.6) for late responding tissues. The median cumulative values of actual rectal (R-BED $Gy_3$) and bladder Point BED (V-BED $Gy_3$) were 118.7 $Gy_3$ (range $48.8\~265.2$) and 126.1 $Gy_3$ (range: $54.9\~267.5$), respectively. MD-BED $Gy_3$ showed a good correlation with rectal (p=0.003), but not with bladder complications (p=0.095). R-BED $Gy_3$ had a very strong association (p=<0.0001), and was more predictive of rectal complications than A-BED $Gy_3$. B-BED $Gy_3$ also showed significance in the prediction of bladder complications in a trend test (p=0.0298). No statistically significant dose-response relationship for pelvic control was observed. The Sandwich and Continuous techniques, which differ according to when the ICR was inserted during the EBRT and due to the physicians preference, showed no differences in the local control and complication rates; there were also no differences in the 3 vs. 5 Gy fraction size of HDR-ICBT. Conclusion: The main reasons optimal dose-fractionation guidelines are not easily established is due to the absence of a dose-response relationship for tumor control as a result of the high-dose gradient of HDR-ICBT, individual differences In tumor responses to radiation therapy and the complexity of affecting factors. Therefore, in our opinion, there is a necessity for individualized tailored therapy, along with general guidelines, in the definitive radiation treatment for cervix cancer. This study also demonstrated the strong predictive value of actual rectal and bladder reference dosing therefore, vaginal gauze packing might be very Important. To maintain the BED dose to less than the threshold resulting in complication, early midline shielding, the HDR-ICBT total dose and fractional dose reduction should be considered.

• The Journal of the Korean Society for Microbiology
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• v.20 no.1
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• pp.91-102
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• 1985

The advantages of enzyme-linked immunosorbent assay(ELISA) are its senstivity and simplicity in detecting IgG, IgM and IgA antibody. To apply ELISA to diagnosis of typhoid fever, antigen such as lipopolysaccharide of Salmonella typhi or killed whole cell must be coated on solid phase. It is easy to coat lipopolysaccharide on ELISA plate but troublesome to purify it. As it is easy to obtain the killed whole cells, the development of the appropriate method by which those antigens of S. typhi are optimally coated on solid phase is needed. To establish the appropriate method, carbonate buffer, methanol or poly-L-lysine was applied as binding substance on polystyrene or polyvinylchloride plate as solid phase when the killed whole cell antigens of S. typhi varided as follows: $10^6$, $10^7$, $10^8$ and $10^9\;cell/ml$. The criteria of the optimal method were determined as follows: 1. The optical density of positive sera is above 1.0(0.6 in IgM) at 1:10 serum dilution and is 0.3(0.2 in IgM) higher than that of negative sera: 2. The O.D. of sera is flat or lowering according to serum dilution: 3. It must be that the O.D. of negative sera is lower than 0.2 at the point of serum dilution where the O.D. of positive sera is higher than 1.0(0.5 in IgM). The results obtained were summarized as follows: 1. The methods which fitted the above criteria were to use poly-L-lysine as binding substance, polyvinylchloride plate as solid phase and $10^7\;cell/ml$ as antigen concentration of S. typhi(poly-L-lysine/polyvinylchloride/$10^7$) and poly-L-lysine/polyvinylchloride/$10^8$ in detecting IgG antibody, methanol/polystyrene/$10^9$, poly-L-lysine/polyvinylchloride/$10^8$ and poly-L-lysine/polyvinylchloride/$10^9$ in IgM and carbonate buffer/polystyrene/$10^8$, carbonate buffer/polystyrene/$10^9$, methanol/polystyrene/$10^8$, methanol/polyvinylchloride/$10^8$, methanol/polyvinylchloride/$10^9$, poly-L-lysine/polyvinylchloride/$10^8$ and poly-L-lysine/polyvinylchloride/$10^9$ in IgA. 2. The coaling method using poly-L-lysine, polyvinylchloride plate and $10^8\;cell/ml$ was best to assay IgG, IgM and IgA antibody all in one. By this method, to assay the each immunoglobulin calss with an appropriate fixed serum dilution, 1:320 dilution was best.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.05a
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• pp.408-413
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• 2004

High speed planning boats also have been required more and more the rational strength analysis and evaluation for the optimal structural design in respect of the structural lightness according to the high speed trend. Even though the suggestion of the simple type equation for the equivalent dynamic pressure is reasonable to design the scantling of ship structure conveniently, many research activities for more reasonable improvement of the simple design pressure, have been continued to suggest the more accurate equivalent static description of tire structural response such as the deflection and stress of hull structure. In this research, we focus on the aluminum bottom stiffened plate structure in which structural scantling is mainly depend on the local loads such as dynamic or impact pressure without other load effects and structural response for the simple dynamic equivalent pressure was investigated through the structural analysis. In order to investigate the structural response of the bottom stiffened plate structure subjected to the dynamic equivalent design pressure, linear and nonlinear structural analysis of the bottom stiffened plate structure of 4.3 ton aluminum planning boat was performed based on the equivalent static applied loads which were derived from the KR regulation and representative one among various dynamic equivalent pressure equations. From above analysis results, we found that the response such as deflection and stress of plate member was similar with the response results of one plate member model with fixed boundary, which was published previous paper and in case of KR design loading, all response of stiffened plate structure were within elastic limit. Through the nonlinear analysis, nearly elastic behavior including the slight geometrical nonlinear response was dominant but plastic local zone was appeared at $85\%$ limit load. Therefore, we can say that through tire linear and nonlinear analysis, this stiffened plate member has no structural strength problem based on the yield criteria in case within $60\%$ limit load except the other strength point of view such as the fatigue and buckling problem.