• KSBB Journal
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• 제25권6호
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• pp.547-552
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• 2010

The recent bloom of a very large jellyfish Nemopilema nomurai has caused a danger to sea fishery and sea bathers. Presently, Nemopilema nomurai is thrown away through a separator system in the sea. The objective of this work was to produce bio-gas from Nemopilema nomurai by using anaerobic digestion. The bio-gas includes the hydrogen or the methane gases. It relates that Nemopilema nomurai is effectually changed into the renewable energy. When the jellyfish biomass was used as an organic carbon source the bio-gases were evolved. The aim of this study was to determine the optimal conditions for hydrogen and methane gases production according to the substrate concentrations of Nemopilema nomurai, optimal culture condition and the sludge-pretreatment without pH control. The optimal culture condition was found to be $35^{\circ}C$ and the heat-treatments of jellyfish was done at $120^{\circ}C$ for 30 min. The production rate of hydrogen and methane gas were found to be 8.8 mL/L/h, 37.2 mL/L/h from 1.5 g of dry Nemopilema nomurai.

• 한국균학회지
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• 제20권1호
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• pp.58-64
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• 1992

Trichoderma 속의 제균종중에서 10균주를 선별하여 protoplast 형성에 관한 제반조건을 검토하였으며 그 결과는 다음과 같았다. 보편적으로 protoplast 형성에 있어서 삼투안정제의 효과는 ${KCI}>(NH_4)_2{SO_4}>NaCl>mannitol>{MgSO}_4$ 순이었으며, 최적농도는 0.6-0.9M 사이에서 결정되었다. Protoplast형성을 위한 DRISELASE의 최적조건은 pH5.0, 효소농도 2%. 반응시간 4시간, 반응온도 $30^{\circ}C$ 이었다. NOVOZYM 234의 최적조건은 pH 5.5, 효소농도 1%,반응시간 3시간, 반응온도 $30^{\circ}C$ 이었다. Protoplast 생성은 대수증식기 초기와 증기사이의 균사체가 최적이었다. Protoplast 생성에 있어서는 DRISELASE가 효과적이었으나 T. longibruchiatum IAM 13107과 T. viride IAM 5141의 경우에는 NOVOZYM 234가 보다 효과적이었다.

• 한국미생물·생명공학회지
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• 제21권1호
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• pp.1-5
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• 1993

춘천시 근교의 토양으로부터 glutathione 생산균주를 분리, 동정하고 glutathione 생산 최적조건을 검토하였다. 분리된 균주는 동정을 행한 결과 Candida sp.로 판단되었다. Glutathione 생산을 위한 Candida sp의 배지조성은 fructose 1.0, yeast extract 4.0, NaCl 0.04, thiamine-HCl5{\mu}g/ml$ 및 L-cysteine 0.04 이었으며 온도는 $25^{\circ}C$, pH 6.0에서 36시간 배양하였을 때 glutathione 생산이 가장 좋았다. Glutathion 생산을 위한 최적배지에서 Candida sp. 균주에의한 glutathione 생산량은 $92{\mu}g/ml$로 YM 배지보다 약 2.5배 정도 증가하였다.

• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.69-74
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• 2000

The medium for erythritol production by Torula sp. in a 500-ml baffled flask was optimized to be 300 g/I sucrose, 10 g/I yeast extract, 3 g/I $KH_2PO_4$, and 10 mg/I $CuSO_4{\cdot}5H_2O{\;}at{\;}34^{\circ}C$ with initial pH of 5.5. Using this optimal medium, erythritol of 166 g/I was obtained after 140 h of cultivation, corresponding to 55.3% of the erythritol yield from sucrose with a productivity of 1.11 g/I/h. Optimal concentrations of carbbon and nitrogen sources in a fermentor were higher than that in a flask due to the higher oxygen supply of the fermentor. Employing the medium containing 300 g/I or 400 g/I sucrose for the determination of optimal C/N ratio, the C/N ratio was found to be more important than the nitrogen concentration for effective erythritol production, The optimal ratio of yeast extract to sucrose (g/g) was 20. The yield and productivity of erythritol were maximal in the medium containing 400 g/I sucrose and 20 g/I yeast extract. when dissolved oxygen in the culture was increased, the cell mass increased but the erythritol production was manimal in the range of 5 to 10% of dissolved oxygen. Under the optimal the rane of 5 to 10% of dissolved oxygen. Under the optimal culture condition of the fermentor, a final erythritol concentration of 200 gI was obtained after 120 h with a yield of 50% and the productivity was 1.67 g/I/h. The yield was the highest among erythritol-producting microorganisms

• 생명과학회지
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• 제23권3호
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• pp.368-376
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• 2013

Helicobacter pylori 억제효과가 우수한 청목노상의 캘러스 배양을 위한 최적조건은 MS 고체배지에서 $27^{\circ}C$에서 20일간 배양하였을 때 6.4 mm의 크기로 가장 크게 자랐으며, 청목노상의 callus 형성에 미치는 생장조절제로는 NAA, 2,4-D, BA 및 kinetin 등을 2 mg/l의 농도로 첨가하여 $27^{\circ}C$에서 20일간 배양했을 때 높은 캘러스 성장률을 확인 할 수 있었고, 생장조절제의 혼합처리구가 단독처리구 보다 캘러스 형성율이 높은 것을 확인 할 수 있다. 따라서 본 연구에 사용된 청목노상 품종의 평판기내 배양을 위한 direct callogenesis의 최적 조건은 생장호르몬으로 2,4-D/NAA를 2 mg/l의 농도로 혼합 처리하여 $27^{\circ}C$에서 20일간 배양이 최적조건이었다. 청목노상 callus로부터 Helicobacter pylori 억제물질의 대량생산을 위한 방법인 biomass를 위한 bioreactor배양은 MS 액체배지에 호르몬으로 2,4-D와 BA를 각각 1 mg/l의 농도로 처리하여 20일간 배양하였을 때가 최적조건이었다. 최적조건에서 배양한 callus 추출물의 Helicobacter pylori 에 대한 억제효과는 16 mm의 clear zone으로 가장 높은 저해율을 확인할 수 있었다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 춘계학술대회
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• pp.403-407
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• 2005

Objective of the research is to demonstrate solar thermal space and ground heating system which is integrated to a greenhouse culture facility for reducing heating cost, increasing the value of product by environment control, and developing advanced culture technology by deploying solar thermal system. Field test for the demonstration was carried out in horticulture complex in Jeju Island. Medium scale solar hot water system was installed in a ground heating culture facility. Reliability and economic aspect of the system which was operated complementary with thermal storage and solar hot water generation were analyzed by investigating collector efficiency, operation performance, and control features. Short term day test on element performance and Long term test of the whole system were carried out. Optimum operating condition and its characteristics were closely investigated by changing the control condition based on the temperature difference which is the most important operating parameter. For establishing more reliable and optimal design data regarding system scale and operation condition, continuous operation and monitoring on the system need to be further carried out. However, it is expected that, in high-insolation areas where large-scale ground storage is adaptable, solar system demonstrated in the research could be economically competitive and promisingly disseminate over various application areas.

• 신재생에너지
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• 제1권2호
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• pp.53-59
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• 2005

Objective of the research is to demonstrate solar thermal space and ground heating system which is integrated to a green-house culture facility for reducing healing cost, Increasing the value of product by environment control, and developing advanced culture technology by deploying solar thermal system. Field test for the demonstration was carried out in horticulture complex In Jeju Island. Medium scale solar hot water system was installed in a ground heating culture facility. Reliability and economic aspect of the system which was operated complementary with thermal storage and solar hot water generation were analyzed by investigating collector efficiency, operation performance, and control features. Short term day test on element performance and Long term test of the whole system were carried out. Optimum operating condition and its characteristics were closely Investigated by changing the control condition based on the temperature difference which Is the most important operating parameter For establishing more reliable and optimal design data regarding system scale and operation condition, continuous operation and monitoring on the system need to be further carried out. However, It is expected that, in high-insolation areas where large-scale ground storage is adaptable, solar system demonstrated in the research could be economically competitive and promisingly disseminate over various application areas.

• Environmental Engineering Research
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• 제20권2호
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• pp.121-125
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• 2015

Biohydrogen production from food waste via dark fermentation was conducted by using mixed culture under various environmental conditions (initial pH, initial F/M ratio, initial ferrous iron ($Fe^{2+}$), and temperature condition) in batch reactor. The results revealed that the maximum hydrogen yield of $46.19mL\;H_2/g\;COD_{add}$ was achieved at the optimal conditions (initial pH 8.0, initial F/M ratio 4.0, initial iron concentration 100 mg $FeSO_4/L$ and thermophilic condition ($55{\pm}1^{\circ}C$)). Furthermore, major volatile fatty acid (VFA) productions of butyrate (765.66 mg/L) and acetate (324.69 mg/L) were detected and COD removal efficiency was detected at 66.00%. Therefore, these optimal conditions could be recommended to operate a system.

• 상하수도학회지
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• 제12권4호
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• pp.78-85
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• 1998

Strains degrading and decolorizing reactive dyes, Procion blue HEGN and Procion red HE7B were isolated from water system, are named as RBK1 and RRK, the growth characteristics of which were investigated. Decolorization efficiencies after 42 hrs in batch culture were 95% and 77%, respectively. and the optimal culture condition of temperature and pH were $30^{\circ}C$, 7.0. Decolorization efficiencies in condition of aerobic shaking culture by strains RBK1 and RRK conspicuously increased, and culture by strain RBK1 was found as 95% after 42 hrs, while standing culture was 64%, Optimum nitrogen source was peptone, and It was found that decolorization efficiencies by strains RBK1 and RRK increased up to 4,000mg/l of peptone concentration as nitrogen source, but peptone concentration did' nt influence the decolorization efficiency in above 4,000mg/l. When the concentration of dyes were more than 800 mg/l and 400 mg/l respectively, the strains RBK1 and RRK, which degrade Procion bule HEGN and Procion red HE7B, showed a sharply decreased decolorization efficiencies; then the specific growth rate were $0.25hr^{-1}$ and $0.09hr^{-1}$.

• KSBB Journal
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• 제14권1호
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• pp.71-75
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• 1999

인 축적능이 우수한 균주를 자연계로부터 위노그라드스키 칼럼을 이용해 분리하여 Acinetobacter 속으로 동정하였고, Aci-netobacter CW3로 망명하였다. 인 축적균주의 배양초기조건은 $20^{\circ}C$. pH 7, 200rpm, 18.5mg $FO_4-P/\ell$가 최적임을 보였다. Acinetobacter CW3은 회분배양에서는 최적조건에서 12시간만에 인의 제거가 완료되었다. 이 균주는 호기조건에서는 인을 균주 내부에 과량 축적하고 첨가조건에서 인을 다시 방출함을 보였다.