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http://dx.doi.org/10.5352/JLS.2013.23.3.368

Production of Inhibitory Compounds against Helicobacter pylori by Culture Condition of Morus alba cv. Cheongmoknosang Callus  

Cho, Young-Je (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University)
Cha, Won-Seup (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University)
Kang, Sun-Ae (School of Applied Bioscience, Kyungpook National University)
An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Hanny University)
Ahn, Dong-Hyun (Department of Food Science & Technology, Institute of Food Science, Pukyong National University)
Kim, Myung-Uk (Gyungbuk Institute for Marine Bio-Industry)
Chae, Jung-Woo (Gyeonggi-do Forest Environment Research Institute)
Publication Information
Journal of Life Science / v.23, no.3, 2013 , pp. 368-376 More about this Journal
Abstract
The optimal condition for Morus alba cv was an MS culture medium at $27^{\circ}C$ for 20 days. Cheongmoknosang callus showed inhibitory activity against Helicobacter pylori at 1.05 g of wet weight of the cultured callus. The callus formation of Morus alba cv. Cheongmoknosang was influenced by naphthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), 6-benzylaminopurine (BA) and kinetin at concentrations of 2 mg/l. The growth rate of callus was higher than it was when these hormones were mixed with a single hormone. Thus, the optimal condition for direct callogenesis was to incubate with mixture (2,4-D/NAA) of 2 mg/l concentration at $27^{\circ}C$ for 20 days. Moreover, the optimal culture condition of the biomass in the mass production of inhibitory compounds against Helicobacter pylori from Morus alba cv. Cheongmoknosang callus was to incubate in an MS broth (each concentration 1 mg/l of 2,4-D and BA). When Morus alba cv. Cheongmoknosang callus were incubated for 20 days in a bioreactor, Helicobacter pylori inhibition of callus extracts was the highest at a clear zone of 16 mm.
Keywords
Callus; Morus alba cv. Cheongmoknosang; culture condition; inhibitory activity; Helicobacter pylori;
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