• Title/Summary/Keyword: optimal composition rate

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Growth and fatty Acid Composition with Growth Conditions for Spirulina platensis platensis (배양 조건에 따른 Spirulina platensis의 성장 및 지방산 조성)

  • Joo Dong-Sik;CHO Man-Gi;Buchholz Rainer;LEE Eung-H
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.3
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    • pp.409-416
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    • 1998
  • Owing to their high growth rate, marine microalgae such as Chlorella, Spirulina, Porphpidium and Dunaliella have been believed to be potentially useful for the production of foods, drugs and energy from light, $CO_2$ and minerals. In this study, we investigated the relationship between the growth and fatty acid composition in the blue green alga Spirulina platensis when the temperature and light intensity of culture conditions were changed. The optimal growth conditions for Spirulina platensis from the biomass and lipid contents were $30^{\circ}C$ on 6391 $\mu$E/$m^3$/sec and $35^{\circ}C$ on 4235 $\mu$E/$m^3$/sec. The difference of lipid contents between exponential phase and stationary phase were very large according to growth conditions. According to growth conditions the fatty acid compositions of Spirulina platensis differed, but regardless of growth conditions the main fatty acids were C16 : 0 and C20 : 0 in saturated fatty acid, C16 : 1, C18 : 1 and C 18 : 2 in unsaturated fatty acid.

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Optimization of biomass production of Acetobacter pasteurianus SRCM101388 (Acetobacter pasteurianus SRCM101388 바이오매스 생산 최적화)

  • Jun-Tae Kim;Sung-Ho Cho;Do-Youn Jeong;Young-Soo Kim
    • Food Science and Preservation
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    • v.30 no.1
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    • pp.132-145
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    • 2023
  • In this study, culture conditions were optimized to confirm the feasibility of Acetobacter pasteurianus as a starter for fermentation vinegar. Acetobacter pasteurianus strain can be used as a food ingredient. The optimal temperature and pH conditions of the selected Acetobacter pasteurianus SRCM101388 were 28℃ and pH 6.00, respectively. The response surface methodology (RSM) was used to optimize the composition of the medium, and Plackett-Burman design (PBD) was used to obtain the effective selection of culture medium, resulting in that glucose, sucrose, and yeast extract had the highest effect on increasing biomass. The optimal concentration, which was performed by central composite design (CCD), were determined to be 10.73 g/L of glucose, 3.98 g/L of sucrose, and 18.73 g/L of yeast extract, respectively. The optimal concentrations of trace elements for the production of biomass were found to be 1 g/L of ammonium sulfate, 0.5 g/L of magnesium sulfate, 2 g/L of sodium phosphate monobasic, 2 g/L of sodium phosphate dibasic, and the final optimized medium was pH 6.10. When incubated in a 5 L jar fermenter, the SRCM101388 strain showed a faster-dissolved oxygen (DO) reduction at a lower agitation rate (rpm), and it was able to grow even at reduced DO level when aeration was maintained. The amount of final biomass produced was 2.53±0.12×109 CFU/mL (9.40±0.02 log CFU/mL) when incubated for 18 hours at 150 rpm, 0.5 vvm, pH 6.0, and 28℃.

An Experimental Study on the Two Stage-Ignition of Cool Flame and Hot Flame in HCCI Engine According to Fuel Composition (연료조성에 따른 HCCI 엔진의 냉염 및 열염의 2단연소 특성에 관한 실험적 연구)

  • Kim, Hyung-Min;Ryu, Jea-Duk;Lee, Ki-Hyung;Lee, Chang-Sik
    • 한국연소학회:학술대회논문집
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    • 2003.05a
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    • pp.13-19
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    • 2003
  • As the environmental pollution becomes serious global problem, the regulation of emission exhausted from automobiles is strengthen. Therefore, it is very important to know how to reduce the NOx and PM simultaneously in diesel engines, which has lot of merits such as high thermal efficiency, low fuel consumption and durability. By this reason, the new concept called as Homogeneous Charge Compression Ignition(HCCI) engines are spotlighted because this concept reduced NOx and P.M. simultaneously. However, it is well known that HCCI engines increased HC and CO. Thus, the investigation of combustion characteristics which consists cool and hot flames for HCCI engines were needed to obtain the optimal combustion condition. In this study, combustion characteristics for direct inject type HCCI engine such as quantity of cool flame and hot flame, ignition timing and ignition delay were investigated to clarify the effects of these parameters on performance. The results revealed that diesel combustion showed the two-stage ignition of cool flame and hot flame, the rate of cool flame increase and hot flame decrease with increasing intake air temperature. On the other hand, the gasoline combustion is the single-stage ignition and ignition timing is near the TDC. In addition mixed fuel combustion showed different phenomenon, which depends on the ratio of gasoline component. Ignition timing of mixed fuel is retarded near the TDC and the ignition delay is increased according to ratio of gasoline.

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Evaluation of Self-Healing Performance Using Hydration Model of Portland Cement and Clinker (포틀랜드시멘트와 클링커의 수화모델을 이용한 자기치유 성능평가)

  • Choi, Sang-Hyeon;Park, Byoung-Sun;Cha, Soo-Won
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.8 no.1
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    • pp.81-87
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    • 2020
  • Crack control is essential to increase the durability of concrete significantly. Healing of crack can be controlled by rehydration of unreacted clinkers at the crack surface. In this paper, by comparing the results of isothermal calorimetry test and regression analysis, the Parrot & Killoh's cement hydration model was verified and clink er hydration model was proposed. The composition and quantification of hydration products were simulated by combining kinematic hydration model and thermodynamic model. Hydration simulation was conducted using the verified and proposed hydration model, and the simulation was performed by the substitution rate of clink er. The type and quantity of the final hydration product and healing product were predicted and, in addition, the optimal cementitious material of self-healing concrete was selected using the proposed hydration model.

Effect of Controlled Light Environment on the Growth and Ginsenoside Content of Panax ginseng C. A. Meyer (광환경 조절이 인삼의 생육과 진세노사이드 함량에 미치는 영향)

  • Jang, In Bae;Yu, Jin;Kweon, Ki Bum;Suh, Su Jeoung
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.4
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    • pp.277-283
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    • 2016
  • Background: The photosynthetic efficiency cool-season, semi-shade ginseng is normal at low morning temperatures, but drops at high afternoon temperatures. Therefore, optimal plant performance would be ensured if it were possible to control daily light transmission rates (LTR). Methods and Results: Plants were grown in a controlled light environment that replicated 11 AM conditions and comparatively analyzed against plant grown under normal conditions. Growth in the controlled light environment resulted in a 2.81 fold increase in photosynthetic efficiency with no change in chlorophyll content, although LTR were high due to low morning temperatures. Increased aerial plant growth was observed in the ginseng plants adapted to the controlled light environment, which in turn influenced root weight. An 81% increase in fresh root weight (33.3 g per plant on average) was observed in 4-year-old ginseng plants grown in controlled light environment compared to the plants grown following conventional practices (18.4 g per plant on average). With regard to the inorganic composition of leaves of 4-year-old ginseng plants grown in controlled light environment, an increased in Fe content was observed, while Mn and Zn content decreased, and total ginsenoside content of roots increased 2.37 fold. Conclusions: Growth of ginseng under a favorable light environment, such as the condition which exist naturally at 11 AM and are suitable for the plant's photosynthetic activity creates the possibility of large scale production, excellent-quality ginseng.

CFD Performance Analysis and Design of a 8kW Class Radial Inflow Turbine for Ocean Thermal Energy Conversion Using a Working Fluid of Ammonia (암모니아 작동유체를 이용한 해수온도차발전용 8kW급 구심터빈의 설계 및 CFD 성능해석)

  • Mo, Jang-Oh;Cha, Sang-Won;Kim, You-Taek;Lim, Tae-Woo;Lee, Young-Ho
    • Journal of Advanced Marine Engineering and Technology
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    • v.36 no.8
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    • pp.1030-1035
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    • 2012
  • In this research, we analysed design and CFD analysis of an inflow radial turbine for OTEC with an output power of 8kW using an working fluid of ammonia. The inflow radial turbine consists of scroll casing, vain nozzle with 18 blade numbers and rotor blade with 13 blade numbers. Mass flow rate, and inlet temperature are 0.5kg/s and $25^{\circ}C$ respectively, and variable rotational speeds were applied between 12,000 and 36,000 with 3,000 rpm intervals. As the results according to the rotational speeds, the designed speed is 24,000 rpm where maximum efficiency exists. The maximum efficiency and output power are 88.66% and 8.52kW, respectively. Through this study, we expect that the analysed results will be used as the design material for the composition of the turbine optimal design parameters corresponding to the target output power under various working material conditions.

Properties in Formation of Protein-Binding Polysaccharide in Cordyceps militaris (동충하초(冬蟲夏草)의 단백다당류 생성 특성)

  • 최용욱;이영엽;정용영;권태영;정용준
    • The Korean Journal of Food And Nutrition
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    • v.17 no.2
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    • pp.177-185
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    • 2004
  • The effects of liquid culture conditions and nutrient sources on the formation of protein-binding polysaccharide (PS) in Cordyceps militaris were examined. The formation amount of PS was increased in proportion to the growth rate of mycelium, in case of higher aeration or lower acidity. The optimum growth temperature of the mycelia was 25$^{\circ}C$ for the formation of PS. The optimum carbon source and nitrogen source were glucose and peptone, respectively. The ratio of C/N was optimal with 3% glucose to 0.5 % peptone. The sugar composition in the PS was greatly changed according to the carbon sources. The mycelium of Cordyceps militaris by liquid culture showed a higher electron donating ability than that by solid culture.

Fatty Acid and Carotenoid Production by Sporobolomyces ruberrimus when Using Technical Glycerol and Ammonium Sulfate

  • Razavi, Seyed Hadi;Mousavi, Seyed Mohammad;Yeganeh, Hassan Mehrabani;Marc, Ivan
    • Journal of Microbiology and Biotechnology
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    • v.17 no.10
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    • pp.1591-1597
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    • 2007
  • The production of carotenoids, lipid content, and fatty acid composition were all studied in a strain of Sporobolomyces ruberrimus when using different concentrations of technical glycerol as the carbon source and ammonium sulfate as the nitrogen source. The total lipids represented an average of 13% of the dry weight, and the maximum lipids were obtained when using 65.5 g/l technical glycerol (133.63 mg/g). The optimal conditions for fatty acid production were at $27^{\circ}C$ using 20 g of ammonium sulfate and a pH range from 6 to 7, which produced a fatty acid yield of $32.5{\pm}1\;mg/g$, including $1.27{\pm}0.15\;mg$ of linolenic acid (LNA), $7.50{\pm}0.45\;mg$ of linoleic acid (LLA), $5.50{\pm}0.35\;mg$ of palmitic acid (PA), $0.60{\pm}0.03\;mg$ of palmitoleic acid (PAL), $1.28{\pm}0.11\;mg$ of stearic acid (SA), $9.09{\pm}0.22\;mg$ of oleic acid, $2.50{\pm}0.10\;mg$ of erucic acid (EA), and $4.25{\pm}0.20\;mg$ of lignoceric acid (LCA), where the palmitic, oleic, and linoleic acids combined formed about 37% of the total fatty acids. The concentration of total carotenoids was 2.80 mg/g when using 20 g of ammonium sulfate, and consisted of torularhodin (2.70 mg/g) and $\beta$-carotene (0.10 mg/g), at $23^{\circ}C$ and pH 6. However, the highest amount with the maximum specific growth rate was obtained (${\mu}_{max}=0.096\;h^{-1}$) with an ammonium sulfate concentration of 30 g/l.

The Comparative Study on Compositional Pattern Analysis of Decoction of Extracted Artemisia argyi by Different Extraction Time (전탕 시간에 따른 애엽의 성분패턴 비교연구)

  • Yoon, Jun-Geol;Kim, Mean-Sun;Han, Seong-Min;Hwang, Deok-Sang;Lee, Jin-Moo;Lee, Chang-Hoon;Jang, Jun-Bock
    • The Journal of Korean Obstetrics and Gynecology
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    • v.33 no.2
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    • pp.1-12
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    • 2020
  • Objectives: This study was conducted to find out the optimal extraction time for Artemisia argyi. Methods: The compositional pattern was compared with HPLC (High Performance Liquid Chromatography) and GC (Gas-Chromatography) by decocting Artemisia argyi 10, 60, 120 minutes respectively. Results: With longer extraction time, the contents of reference compounds were extracted 1.1 times more when 3,4-dicaffeoylquinic acid was extracted for 60 minutes than when extracted for 10 minutes in HPLC test, but the contents were reduced when extracted for 120 minutes compared to 60 minutes extraction time. 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, jaceosidin, and eupatilin showed the largest yield rate when extracted for 10 minutes, and it decreased as time passed. The contents of chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, jaceosidin, scoparone, and eupatilin were detected only in 10 minutes extraction but not in 60 or 120 minutes extraction according to GC test. Conclusions: The results show that extraction time could affect the physicochemical characteristic or composition of Artemisia argy extracted. Thus, short extraction time could be useful for decoction of Artemisia argyi.

A Study on Performance Evaluation for the Bio-retention Non-point Source Pollution Treatment System (생물 저류 방법 적용을 통한 비점오염원 처리시설의 성능평가에 관한 연구)

  • Lee, Jang-Soo;Park, Yeon-Soo;Cho, Wook-Sang
    • Clean Technology
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    • v.19 no.3
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    • pp.295-299
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    • 2013
  • This study was purposed and performed to evaluate removal efficiency of non-point source pollution in the process and system based on bio-retention design criteria regulated by EPA. Basic Column Reactors (BCR) were prepared for optimal determinations of inflow rate of first rainfall runoff and composition and ratio of soil layers. Removal efficiencies of non-point source pollution from synthetic runoff and real first rainfall runoff, directly sampled from motor way and parking lot, were analyzed, respectively. Removal efficiency of SS, BOD, COD, T-N, and T-P were all shown to be more than 80%.