• BMB Reports
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• 제52권11호
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• pp.641-646
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• 2019

The Ron proto-oncogene is a human receptor for macrophage-stimulating protein (MSP). The exclusion of exon 11 in alternative splicing generates ${\Delta}RON$ protein that is constitutively activated. Heterogenous ribonucleaoprotein (hnRNP) $C_1/C_2$ is one of the most abundant proteins in cells. In this manuscript, we showed that both hnRNP $C_1$ and $C_2$ promoted exon 11 inclusion of Ron pre-mRNA and that hnRNP $C_1$ and hnRNP $C_2$ functioned independently but not cooperatively. Moreover, hnRNP $C_1$ stimulated exon 11 splicing through intron 10 activation but not through intron 11 splicing. Furthermore, we showed that, whereas the RRM domain was required for hnRNP $C_1$ function, the Asp/Glu domain was not. In conclusion, hnRNP $C_1/C_2$ promoted exon 11 splicing independently by stimulating intron 10 splicing through RRM but not through the Asp/Glu domain.

• Molecules and Cells
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• 제24권2호
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• pp.276-282
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• 2007

Protein phosphorylation is one of the major mechanisms by which eukaryotic cells transduce extracellular signals into intracellular responses. Calcium/calmodulin ($Ca^{2+}/CaM$)-dependent protein phosphorylation has been implicated in various cellular processes, yet little is known about $Ca^{2+}/CaM$-dependent protein kinases (CaMKs) in plants. From an Arabidopsis expression library screen using a horseradish peroxidase-conjugated soybean calmodulin isoform (SCaM-1) as a probe, we isolated a full-length cDNA clone that encodes AtCK (Arabidopsis thaliana calcium/calmodulin-dependent protein kinase). The predicted structure of AtCK contains a serine/threonine protein kinase catalytic domain followed by a putative calmodulin-binding domain and a putative $Ca^{2+}$-binding domain. Recombinant AtCK was expressed in E. coli and bound to calmodulin in a $Ca^{2+}$-dependent manner. The ability of CaM to bind to AtCK was confirmed by gel mobility shift and competition assays. AtCK exhibited its highest levels of autophosphorylation in the presence of 3 mM $Mn^{2+}$. The phosphorylation of myelin basic protein (MBP) by AtCK was enhanced when AtCK was under the control of calcium-bound CaM, as previously observed for other $Ca^{2+}/CaM$-dependent protein kinases. In contrast to maize and tobacco CCaMKs (calcium and $Ca^{2+}/CaM$-dependent protein kinase), increasing the concentration of calmodulin to more than $3{\mu}M$ suppressed the phosphorylation activity of AtCK. Taken together our results indicate that AtCK is a novel Arabidopsis $Ca^{2+}/CaM$-dependent protein kinase which is presumably involved in CaM-mediated signaling.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.61-61
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• 2015

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive diseases of rice worldwide. The rice - M. oryzae pathosystem has become a model in the study of plant - fungal interactions due to its economic importance and accumulating knowledge. During the evolutionary arms race with M. oryzae, rice plants evolved a repertoire of Resistance (R) genes to protect themselves from diseases in a gene-for-gene fashion. M. oryzae secretes a battery of small effector proteins to manipulate host functions for its successful infection, and some of them are recognized by host R proteins as avirulence effectors (AVR), which turns on strong immunity. Therefore, the analysis of interactions between AVRs and their cognate R proteins provide crucial insights into the molecular basis of plant - fungal interactions. Rice blast resistance genes Pik, Pia, Pii comprise pairs of protein-coding ORFs, Pik-1 and Pik-2, RGA4 and RGA5, Pii-1 and Pii-2, respectively. In all three cases, the paired genes are tightly linked and oriented to the opposite directions. In the AVR-Pik/Pik interaction, it has been unraveled that AVR-Pik binds to the N-terminal coiled-coil domain of Pik-1. RGA4 and RGA5 are necessary and sufficient to mediate Pia resistance and recognize the M. oryzae effectors AVR-Pia and AVR1-CO39. A domain at the C-terminus of RGA5 characterized by a heavy metal associated domain was identified as the AVR-binding domain of RGA5. Similarly, physical interactions among Pii-1, Pii-2 and AVR-Pii are being analyzed.

• 한국자기공명학회논문지
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• 제19권2호
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• pp.74-82
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• 2015

Ryanodine receptor (RyR) is one of the two major $Ca^{2+}$ channels in membranes of intracellular $Ca^{2+}$ stores and is found in sarcoplasmic reticulum (SR), endoplasmic reticulum (ER). RyR1 is also the major calmodulin-binding protein of sarcoplasmic reticulum membranes. Residues 4064-4210 in the RyR1 polypeptide chain has similar primary sequence with calmodulin (CaM) and was designated as CaM-like domain (CaMLD). When expressed as a recombinant peptide, CaMLD showed several CaM-like properties in previous studies. Still, previous studies of CaMLD were focused on protein-protein interactions rather than its own properties. Here, we studied the expression of CaMLD and its sub-domains corresponding to each lobe of CaM in Escherichia coli. CaMLD could be obtained only as inclusion body, and it was refolded using urea solubilization followed by dialysis. Using spectroscopic approaches, such as NMR, circular dichroism, and gel filtration experiment, we found that the refolded CaMLD exists as nonspecific aggregate, even though it has alpha helical secondary structure. In comparison, the first half of CaMLD (R4061-4141) could be obtained as natively soluble protein with thioredoxin fusion. After the removal of the fusion tag, it exhibited folded and helical properties as shown by NMR and circular dichroism experiments. Its oligomeric status was different from CaMLD, existing as dimeric form in solution. However, the second half of the protein could not be obtained as soluble protein regardless of fusion tag. Based on these results, we believe that CaMLD, although similar to CaM in sequence, has quite different physicochemical properties and that the second half of the protein renders it the aggregative properties.

• 대한수학회논문집
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• 제37권2호
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• pp.585-594
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• 2022

In this article we classify four dimensional gradient Ricci solitons (M, g, f) with half harmonic Weyl curvature and at most two distinct Ricci-eigenvalues at each point. Indeed, we showed that, in a neighborhood V of each point in some open dense subset of M, (V, g) is isometric to one of the following: (i) an Einstein manifold. (ii) a domain in the Riemannian product (ℝ², g₀) × (N, ${\tilde{g}}$), where g₀ is the flat metric on ℝ² and (N, ${\tilde{g}}$) is a two dimensional Riemannian manifold of constant curvature λ ≠ 0. (iii) a domain in ℝ × W with the warped product metric $ds^2+h(s)^2{\tilde{g}}$, where ${\tilde{g}}$ is a constant curved metric on a three dimensional manifold W.

• 한국토양환경학회지
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• 제3권1호
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• pp.83-92
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• 1998

본 연구는 가스 포집관을 설치한 쓰레기 매립지에서의 가스 유동에 따른 모델 해석으로 부터 다음의 결과를 얻었다. 1) 저항이 없는 유량 계측기의 오차는, 투기계수 $10^{-14}$ $m^{2}$(실트; 포화투수계수 $10^{-7}$ m/s)보다 작은 복토를 쓰고 가스 발생량을 계측한다면, \circled1 직경 l0m 내에서는 0.5 mL/s 이상, \circled2 직경 20m 내에서는 2mL/s 이상, \circled3 직경 50m 내에서는 10 mL/s 이상의 유량범위에서, 정확한 계측치를 얻을 수 있다. 2)복토층의 \circled1투기계수가 $10^{-16}$$m^{2}$(점토; 포화투수계수 $10^{-9}$( m/s)에서는, 3종(bubblemeter, water head indicator and rotor meter)의 유량 계측기 모두 50m까지 유효한 계측이 가능하다. \circled2투기계수가 1$10^{-14}$$m^{2}$에서는, bubble meter and water head indicator는 5m 이하, rotor meter는 15m 내의 범위에서 유효한 계측이 가능하다. \circled3투기계수가 $10^{12}$$m^{2}$에서는 가스 포집관의 2m범위 이내에서만 유효히 계측된다. 3) 가스 포집관에 유량 계측기를 설치하면, 계측조건의 변화로 정상조건까지의 도달시간은 bubble meter와 water head indicator는 약 1일, roter meter는 약 1시간 정도가 소요된다.

• Journal of the Optical Society of Korea
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• 제15권2호
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• pp.111-117
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• 2011

Full-range spectral domain optical coherence tomography (SD-OCT) with a 1-${\mu}m$ band light source is shown here. The phase of the reference beam is continuously stepped while the probing beam scans the sample laterally (B-scan). The two dimensional spectral interferogram obtained is processed by a Fourier transform method to obtain a complex spectrum leading to a full-range OCT image. A detailed mathematical explanation of the complex conjugate resolving method utilized is provided. The system's measurement speed was 7.96 kHz, the measured axial resolution was $9.6{\mu}m$ in air and the maximum sensitivity 99.4 dB. To demonstrate the effect of mirror image elimination, In vivo human eye pathology was measured.

• 생명과학회지
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• 제15권3호
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• pp.479-485
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• 2005

2002년 2월부터 2005년 2월까지 성인 및 소아 호홉기질환자 994명의 상기도 도말물에서 M. pneumoniae 균주를 분리하고, 분리 균주의 ciprofloxacin, ofloxacin, minecline, tetracycline, sparfloxacin, josamycin, erythromycin에 대한 감수성 검사를 실시하였으며, 분리된 균주의 235 rRNA domain II와 V에서 erythromycin저항성 변이가 일어났는가를 PCR과 유전자 염기서열분석으로 erythromycin에 감수성인 M. pneumonine균주의 염기서열과 비교분석하여 확인하였다. 호흡기질환자에서 M. pneumoniae의 분리율은 123/994$(12.4\%)$이었으며, 분리된 M. pneumoniae 균주의 minocycline, sparfloxacin, tetracycline, ciprofloxacin, ofloxacin, josamycin, erythromycin MIC 범위는 각각 $0.015\~0.25,\;0.06\~0.5,\;0.06\~0.5,\;0.25\~0.5,\;0.25\~0.5,\;0.015\~8.0,\;0.015\~8.0{\mu}m$이었다. 분리 동정된 M. pneumoniae 균주 중에서 erythromycin에 저항성인 균주가 60주$(48.8\%)$였으며, 모두가 ribosomal protein L4 영역과 23S rRNA domain V에 내성변이가 일어났으며, 이 중 2균주는 23S rRNA domain II에도 변이가 일어난 균주도 있었다. 국내에서 분리되는 M. pneumoniae균주의 $48\%$가 erytomycin에 저항성인 균주이므로 앞으로 이 균에 의한 폐렴의 치료에 주의가 요구된다.

• 한국자기학회지
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• 제5권5호
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• pp.663-666
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• 1995

The domain wall motion coercivity, $H_{c}$, of magnetic materials arises from the dependence of the wall energy on localized changes in material parameters (magnetization, anisotropy, exchange energy densities). However, in an otherwise perfectly homogeneous material, the domain wall energy might change due to the change in the volume of the wall versus the wall position. Thus, any surface roughness contributes to the coercivity. Assuming different two-dimensional surface profiles, characterized by average wavelengths ${\lambda}_{x}$ and ${\lambda}_{y}$, and relative thickness variations dh/h, the coercivity due to the surface roughness has been calculated. Compared to the one dimensional case, the 2D coercivity is reduced. Depending on the ratio of ${\lambda}$ to the domain wall width, $H_{c}$ has a maximum around 2, and increasing with dh/h. With the decreasing thickness of the thin film and GMR heads, it might be the domain factor in determining the coercivity.

• 한국전자파학회논문지
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• 제27권6호
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• pp.531-538
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• 2016

본 논문에서는 2중의 송수신 모듈을 이용하여 2차원 상의 표적 위치를 탐지하고, 실시간으로 추적할 수 있는 근거리 FMCW 레이다를 설계 및 제작하였다. 시분할로 동작하는 송수신 모듈로부터 수집된 1차원 거리 정보를 교차 활용하여 2차원 위치를 추출하는 기법을 제안하였고, 2차원 근거리 레이다에 성공적으로 적용하였다. 개별 레이다의 송수신 모듈에 대한 성능 측정 결과로 10 m와 30 m 위치에 있는 표적에 대한 결과를 제시하였으며, 주파수 분해능에 대응하는 range bin을 이용한 2차원 표적 맵을 활용하여 2차원 레이다의 성능 검증이 수행되었고, 제안된 기법의 유효성을 확인하였다.