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http://dx.doi.org/10.5483/BMBRep.2019.52.11.080

RRM but not the Asp/Glu domain of hnRNP C1/C2 is required for splicing regulation of Ron exon 11 pre-mRNA  

Moon, Heegyum (School of Life Sciences, Gwangju Institute of Science and Technology)
Jang, Ha Na (School of Life Sciences, Gwangju Institute of Science and Technology)
Liu, Yongchao (School of Life Sciences, Gwangju Institute of Science and Technology)
Choi, Namjeong (School of Life Sciences, Gwangju Institute of Science and Technology)
Oh, Jagyeong (School of Life Sciences, Gwangju Institute of Science and Technology)
Ha, Jiyeon (School of Life Sciences, Gwangju Institute of Science and Technology)
Kim, Hyeon Ho (Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University)
Zheng, Xuexiu (School of Life Sciences, Gwangju Institute of Science and Technology)
Shen, Haihong (School of Life Sciences, Gwangju Institute of Science and Technology)
Publication Information
BMB Reports / v.52, no.11, 2019 , pp. 641-646 More about this Journal
Abstract
The Ron proto-oncogene is a human receptor for macrophage-stimulating protein (MSP). The exclusion of exon 11 in alternative splicing generates ${\Delta}RON$ protein that is constitutively activated. Heterogenous ribonucleaoprotein (hnRNP) $C_1/C_2$ is one of the most abundant proteins in cells. In this manuscript, we showed that both hnRNP $C_1$ and $C_2$ promoted exon 11 inclusion of Ron pre-mRNA and that hnRNP $C_1$ and hnRNP $C_2$ functioned independently but not cooperatively. Moreover, hnRNP $C_1$ stimulated exon 11 splicing through intron 10 activation but not through intron 11 splicing. Furthermore, we showed that, whereas the RRM domain was required for hnRNP $C_1$ function, the Asp/Glu domain was not. In conclusion, hnRNP $C_1/C_2$ promoted exon 11 splicing independently by stimulating intron 10 splicing through RRM but not through the Asp/Glu domain.
Keywords
Exon 11 inclusion; hnRNP $C_1/C_2$; Pre-mRNA splicing; Ron proto-oncogene; RRM domain;
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