• Molecules and Cells
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• v.37 no.4
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• pp.286-294
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• 2014

Mammalian polo-like kinase 1 (Plk1) has been studied intensively as a key regulator of various cell cycle events that are critical for proper M-phase progression. The polobox domain (PBD) present in Plk1's C-terminal noncatalytic region has been shown to play a central role in targeting the N-terminal kinase domain of Plk1 to specific subcellular locations. Subsequent studies reveal that PBD binds to a phosphorylated motif generated by one of the two mechanisms - self-priming by Plk1 itself or non-self-priming by a Pro-directed kinase, such as Cdc2. Here, we comparatively review the differences in the biochemical steps of these mechanisms and discuss their physiological significance. Considering the diverse functions of Plk1 during the cell cycle, a better understanding of how the catalytic activity of Plk1 functions in concert with its cisacting PBD and how this coordinated process is intricately regulated to promote Plk1 functions will be important for providing new insights into different mechanisms underlying various Plk1-mediated biological events that occur at the multiple stages of the cell cycle.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.1070-1083
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• 2015

A gene (GT-SM3B) encoding a thermostable secreted oligoendopeptidase (GT-SM3B) was cloned from the thermophile Geobacillus thermoleovorans DSM 15325. GT-SM3B is 1,857 bp in length and encodes a single-domain protein of 618 amino acids with a 23-residue signal peptide having a calculated mass of 67.7 kDa after signal cleavage. The deduced amino acid sequence of GT-SM3B contains a conservative zinc metallopeptidase motif (His⁴⁰⁰-Glu⁴⁰¹-X-XHis⁴⁰⁴). The described oligopeptidase belongs to the M3B subfamily of metallopeptidases and displays the highest amino acid sequence identity (40.3%) to the oligopeptidase PepF_Ba from mesophilic Bacillus amyloliquefaciens 23-7A among the characterized oligopeptidases. Secretory production of GT-SM3B was used, exploiting successful oligopeptidase signal peptide recognition by Escherichia coli BL21 (DE3). The recombinant enzyme was purified from the culture fluid. Homodimerization of GT-SM3B was determined by SDS-PAGE. Both the homodimer and monomer were catalytically active within a pH range of 5.0–8.0, at pH 7.3 and 40℃, showing the K_m, V_max, and k_cat values for carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala-OH peptidolysis to be 2.17 ± 0.04 × 10^-6 M, 2.65 ± 0.03 × 10^-3 µM/min, and 5.99 ± 0.07 s^-1, respectively. Peptidase remained stable at a broad pH range of 5.0–8.0. GT-SM3B was thermoactive, demonstrating 84% and 64% of maximum activity at 50℃ and 60℃, respectively. The recombinant oligopeptidase is one of the most thermostable M3B peptidase, retaining 71% residual activity after incubation at 60℃ for 1 h. GT-SM3B was shown to hydrolyze a collagenous peptide mixture derived from various types of collagen, but less preferentially than synthetic hexapeptide. This study is the first report on an extracellular thermostable metallo-oligopeptidase.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.905-912
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• 2013

A novel species, Metschnikowia cibodasensis, is proposed to accommodate eight strains (ID03-$0093^T$, ID03-0094, ID03-0095, ID03-0096, ID03-0097, ID03-0098, ID03-0099, and ID03-0109) isolated from flowers of Saurauia pendula, Berberis nepalensis, and Brunfelsia americana in Cibodas Botanical Garden, West Java, Indonesia. The type strain of M. cibodasensis is ID03-$0093^T$ (= NBRC $101693^T$ =UICC $Y-335^T$ = BTCC-$Y25^T$). The common features of M. cibodasensis are a spherical to ellipsoidopedunculate shaped ascus, which contains one or two needle-shaped ascospores, and lyse at maturity. Asci generally develop directly from vegetative cells but sometimes from chlamydospores. The neighbor-joining tree based on the D1/D2 domain of nuclear large subunit (nLSU) ribosomal DNA sequences strongly supports that M. cibodasensis (eight strains) and its closest teleomorphic species, M. reukaufii, are different species by a 100% bootstrap value. The type strain of M. cibodasensis, ID03-$0093^T$, differed from M. reukaufii NBRC $1679^T$ by six nt (five substitutions and one deletion) in their D1/D2 region of nLSU rDNA, and by 18 nt (five deletions, four insertions, and nine substitutions) in their internal transcribed spacer regions of rDNA, respectively. Four strains representative of M. cibodasensis (ID03-$0093^T$, ID03-0095, ID03-0096, and ID03-0099) showed a mol% G+C content of $44.05{\pm}0.25%$, whereas that of M. reukaufii NBRC $1679^T$ was 41.3%. The low value of DNA-DNA homology (5-16%) in four strains of M. cibodasensis and M. reukaufii NBRC $1679^T$ strongly supported that these strains represent a distinct species.

• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.97-105
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• 2019

Dehydration Responsive Element Binding (DREB) gene is one of the essential transcription factors plants use for responding to stress conditions including salinity, drought, and cold stress. The purpose of this study was to isolate the full length and characterize the DREB gene from three different genotypes of sugarcane, wild, commercial cultivar, and interspecific hybrid sugarcane. The length of the gene, designated ScDREB was 789 bp, and coding for a putative polypeptide of 262 amino acid residues. Sequences of the gene were submitted to the GenBank database with accession numbers of KX280722.1, KX280721.1, and KX280719.1 for wild sugarcane, commercial cultivar (KPS94-13), and interspecific hybrid (Biotec2), respectively. In silico characterization indicated that the deduced polypeptide contains a putative nuclear localization signal (NLS) sequence, and a conserved AP2/ERF domain of the DREB family, at 82-140 amino residues. Based on multiple sequence alignment, sequences of the gene from the three sugarcane genotypes were classified in the DREB2 group. Gene expression analysis indicated, that ScDREB2 expression pattern in tested sugarcane was up-regulated by salt stress. When the plants were under 100 mM NaCl stress, relative expressions of the gene in leaves was higher than those in roots. In contrast, under 200 mM NaCl stress, relative expressions of the gene in roots was higher than those in leaves. This is the first report on cloning the full length and characterization, of ScDREB2 gene of sugarcane. Results indicate that ScDREB2 is highly responsive to salt stress.

• Steel and Composite Structures
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• v.41 no.5
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• pp.761-773
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• 2021

This study presents a 3D non-linear finite element (FE) assessment of dynamic soil-structure interaction (SSI). The numerical investigation has been performed on the time domain through a Finite Element (FE) system, while considering the nonlinear behavior of soil and the multi-directional nature of genuine seismic events. Later, the FE outcomes are analyzed to the recorded in-situ free-field and structural movements, emphasizing the numerical model's great result in duplicating the observed response. In this work, the soil response is simulated using an isotropic hardening elastic-plastic hysteretic model utilizing HSsmall. It is feasible to define the non-linear cycle response from small to large strain amplitudes through this model as well as for the shift in beginning stiffness with depth that happens during cyclic loading. One of the most difficult and unexpected tasks in resolving soil-structure interaction concerns is picking an appropriate ground motion predicted across an earthquake or assessing the geometrical abnormalities in the soil waves. Furthermore, an artificial neural network (ANN) has been utilized to properly forecast the non-linear behavior of soil and its multi-directional character, which demonstrated the accuracy of the ANN based on the RMSE and R² values. The total result of this research demonstrates that complicated dynamic soil-structure interaction processes may be addressed directly by passing the significant simplifications of well-established substructure techniques.

• The Transactions of the Korea Information Processing Society
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• v.5 no.11
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• pp.2845-2854
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• 1998

Median filter can be implemented in the binary domain based on threshold decomposition, stacking property, and linear separability. In this paper, we develop one-dimensional and two-dimensional parallel algorithms for the median filter on a reconfigurable mesh with buses(RMESH) which is suitable for VLSI implementation. And we evaluate their performance by comparing the time complexities of RMESH algorithms with those of algorithms on mesh-connected computer. When the length of M-valued 1-D signal is N and w is the window width, the RMESH algorithm is done in O(Mw) time and mesh algorithm is done in $O(Mw^2)$ time. Beside, when the size of M-valued 2-D image is $N{\times}N$ and the window size is $w{\times}w$, our algorithm on $N{\times}N$ RMESH can be computed in O(Mw) time which is a significant improvement over the $O(Mw^2)$ complexity on $N{\times}N$ mesh.

• Korean Journal of Adult Nursing
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• v.22 no.2
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• pp.211-220
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• 2010

Purpose: This study was done to identify the relationship of trait anger, health state, physical symptoms. and general characteristics to physical symptoms and to identify factors affecting physical symptoms of elderly in urban areas. Methods: The research design for this study was a descriptive survey design using a convenience sampling. Elders (n=276), who agreed to participate in this study completed a self-reporting questionnaire. The collected data were analyzed by descriptive statistics, t-test, ANOVA, Pearson's correlation coefficients, and multiple regression. Results: Study participants reported low trait anger (M=18.61), physical symptoms (M=7.15), and moderate health state (M=3.30). The 45.4%of variance in physical symptoms was significantly explained by emotional function health state (${\beta}$=-.284, p=.013), which is one of the sub-domain of the elderly health state, and trait anger (${\beta}$=3.841, p<.001). Conclusion: Findings of this study provide that the most important factors in explaining physical symptoms for the elders in Korea were emotional function health state and trait anger. Based on the findings of this study, further nursing practice and nursing research for the elders with physical symptoms should be focused on emotional support.

• Journal of the Korean Magnetics Society
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• v.5 no.1
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• pp.27-33
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• 1995

The microwave absorbing characteristics and complex permeability of $Ni_{2-x}Zn_{x}Y$ - rubber composites with variation of composition and particle size were investigated. With a ferrite particle with a diameter of about $1\mu\textrm{m}$, only spin rotational resonance was observed. This behavior probably due to the particle being sufficiently small to approach single domain characteristics so that only spin rotations can occur. The first matching frequency, found in $Ni_{2-x}Zn_{x}Y$ - rubber composites, which was higher than that of spin rotational resonance, increased with spin rotational resonance frequency. It is also found that the second matching frequency is independent of spin rotational resonance frequency. Based on these findings, it could be concluded that the microwave absorbing characteristics were caused by only one type of resonance, the spin rotational resonance.

• Current Optics and Photonics
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• v.1 no.1
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• pp.65-72
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• 2017

We propose a nearly lossless, compact, electrically modulated vertical directional coupler, which is based on the controllable evanescent coupling in a previously proposed graphene-assisted total internal reflection (GA-FTIR) scheme. In the proposed device, two single-mode waveguides are separate by graphene-$SiO_2$-graphene layers. By changing the chemical potential of the graphene layers with a gate voltage, the coupling strength between the waveguides, and hence the coupling length of the directional coupler, is controlled. Therefore, for a properly chosen, fixed device length, when an input wave is launched into one of the waveguides, the ratio of their output powers can be controlled electrically. The operation of the proposed device is analyzed, with the dispersion relations calculated using a model of a one-dimensional slab waveguide. The supermodes in the coupled waveguide are calculated using the finite-element method to estimate the coupling length, realistic devices are designed, and their performance was confirmed using the finite-difference time-domain method. The designed $3{\mu}m$ by $1{\mu}m$ device achieves an insertion loss of less than 0.11 dB, and a 24-dB extinction ratio between bar and cross states. The proposed low-loss device could enable integrated modulation of a strong optical signal, without thermal buildup.

• Biodesign
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• v.5 no.3
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• pp.122-125
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• 2017

Receptor for advanced glycation end products (RAGE) is one of the single transmembrane domain containing receptors and causes various inflammatory diseases including diabetes and atherosclerosis. RAGE extracellular domain has three consecutive IgG-like domains (V-C₁-C₂ domain) which interact with various soluble ligands including heparan sulfate or HMGB1. Studies have shown that each ligand induces different oligomeric forms of RAGE which results in a ligand-specific signal transduction. The structure of mouse RAGE bound to heparan sulfate has been previously determined but the electron density map of heparan sulfate was too ambiguous that the exact position of heparin sulfate could not be defined. Furthermore, the complex structure of human RAGE and heparin sulfate still remains elusive. Therefore, to determine the structure, human RAGE was overexpressed using bacterial expression system and crystallized using the sitting drop method in the condition of 0.1 M sodium acetate trihydrate pH 4.6, 8 % (w/v) polyethylene glycol 4,000 at 290 K. The crystal diffracted to 3.6 Å resolution and the space group is C121 with unit cell parameters a= 206.04 Å, b= 68.64 Å, c= 98.73 Å, α= 90.00°, β= 90.62°, γ= 90.00°.