Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isolation, characterization and expression of transcription factor ScDREB2 from wild, commercial and interspecific hybrid sugarcane in salinity condition

  • Chanprame, Sontichai (Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus) ;
  • Promkhlibnil, Tanawan (Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus) ;
  • Suwanno, Sakulrat (Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus) ;
  • Laksana, Chanakan (Faculty of Agricultural Technology, Burapha University Sakaeo Campus)
  • Received : 2019.04.26
  • Accepted : 2019.05.27
  • Published : 2019.06.30
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Abstract

Dehydration Responsive Element Binding (DREB) gene is one of the essential transcription factors plants use for responding to stress conditions including salinity, drought, and cold stress. The purpose of this study was to isolate the full length and characterize the DREB gene from three different genotypes of sugarcane, wild, commercial cultivar, and interspecific hybrid sugarcane. The length of the gene, designated ScDREB was 789 bp, and coding for a putative polypeptide of 262 amino acid residues. Sequences of the gene were submitted to the GenBank database with accession numbers of KX280722.1, KX280721.1, and KX280719.1 for wild sugarcane, commercial cultivar (KPS94-13), and interspecific hybrid (Biotec2), respectively. In silico characterization indicated that the deduced polypeptide contains a putative nuclear localization signal (NLS) sequence, and a conserved AP2/ERF domain of the DREB family, at 82-140 amino residues. Based on multiple sequence alignment, sequences of the gene from the three sugarcane genotypes were classified in the DREB2 group. Gene expression analysis indicated, that ScDREB2 expression pattern in tested sugarcane was up-regulated by salt stress. When the plants were under 100 mM NaCl stress, relative expressions of the gene in leaves was higher than those in roots. In contrast, under 200 mM NaCl stress, relative expressions of the gene in roots was higher than those in leaves. This is the first report on cloning the full length and characterization, of ScDREB2 gene of sugarcane. Results indicate that ScDREB2 is highly responsive to salt stress.

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References

  1. Agarwal P, Agarwal PK, Nair S, Sopory SK, Reddy MK (2007) Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and its phosphorylation negatively regulates its DNA-binding activity. Mol Genet Genomics 277:189-98 https://doi.org/10.1007/s00438-006-0183-z
  2. Armas C, Padilla FM, Pugnaire FI, Jackson RB (2010) Hydraulic lift and tolerance to salinity of semiarid species: Consequences for species interactions. Oecologia 162:11-21 https://doi.org/10.1007/s00442-009-1447-1
  3. Arunin S (1984) Characteristics and management of salt affected soil in the Northeast of Thailand, p.336-351. In: Ecology and Management of Problem Soils in Asia, Food and Fertilizer Technology Center for the Asian and Pacific Region, Taipei, Rep of China
  4. Ba ANN, Pogoutse A, Provart N, Moses AM (2009) NLStradamus: A simple hidden markov model for nuclear localization signal prediction. BMC Bioinform 10(1):202 https://doi.org/10.1186/1471-2105-10-202
  5. Bouaziz D, Pirrello J, Amor HB, Hammami A, Charfeddine M, Dhieb A, Bouzayen M, Gargouri-Bouzid R (2012) Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato. Plant Physiol Biochem 60:98-108 https://doi.org/10.1016/j.plaphy.2012.07.029
  6. Chen J, Xia X, Yin W (2009) Expression profiling and functional characterization of a DREB2-type gene from Populus euphratica. Biochem Biophys Res Commun 378:483-487 https://doi.org/10.1016/j.bbrc.2008.11.071
  7. Chen M, Wang Q-Y, Cheng X-G, Xu Z-S, Li L-C, Ye X-G, Xia L-Q, Ma Y-Z (2007) GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants. Biochem Biophys Res Commun 353:299-305 https://doi.org/10.1016/j.bbrc.2006.12.027
  8. Chung E, Kim K, Lee JH (2012) Molecular cloning and characterization of a soybean GmMBY184 induce by abiotic stress. J Plant Bitechnol 39:175-181 https://doi.org/10.5010/JPB.2012.39.3.175
  9. Cui M, Zhang W, Zhang Q, Xua Z, Zhu Z, Duanb F, Wu R (2011) Induced over-expression of the transcription factor OsDREB2A improves drought tolerance in rice. Plant Physiol Biochem 49:1384-1391 https://doi.org/10.1016/j.plaphy.2011.09.012
  10. de Lira RM, Silva EF de F e Neto, Simoes DE, Santos Junio JA, Lima BL de C, da Silva JS (2018) Growth and yield of sugarcane irrigated with brackish water and leaching fractions. Agriambi 22:170-175
  11. Guo J, Ling H, Wu Q, Xu L, Que Y (2014) The choice of reference genes for assessing gene expression in sugarcane under salinity and drought stresses. Sci Rep 4: 7042 DOI: 101038/srep07042 https://doi.org/10.1038/srep07042
  12. Hoagland, DR, Arnon DI. (1950) The Water Culture Method for Growing Plants without Soil. Circular 347, University of California, College of Agriculture, Berkeley, USA
  13. Huang L, Wang Y, Wang W, Zhao X, Qin Q, Sun F, Hu F, Zhao Y, Li Z, Fu B, Li Z (2018) Characterization of transcription factor gene OsDRAP1 conferring drought tolerance in rice. Front Plant Sci 9:94 DOI: 103389/fpls201800094 https://doi.org/10.3389/fpls.2018.00094
  14. Kaewjiw N, Laksana C, Chanprame S (2018) Cloning and identification of salt overly sensitive (SOS1) gene of sugarcane. Int J Agric Biol 20:1569-1574
  15. Laksana C, Chanprame S (2015) A simple and rapid method for RNA extraction from young and mature leaves of oil palm (Elaeis guineensis Jacq). ISSAAS 21:96-106
  16. Laksana C, Chanprame S (2017) Partial cloning and expression of ScBADH and ScMIPS gene in wild and cultivated sugarcane under mimicking saline soil conditions. ISSAAS 23:183-192
  17. Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391-1406 https://doi.org/10.1105/tpc.10.8.1391
  18. Livak, KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402-408 https://doi.org/10.1006/meth.2001.1262
  19. Maas EV, Hoffman GJ (1977) Crop salt tolerance - current assessment. J Irrig Drain Eng 103:115-134 https://doi.org/10.1061/JRCEA4.0001137
  20. Mahajan, ST, Naik RM, Dalvi US (2013) Assessment of biochemical markers in differentiating sugarcane genotypes for salt tolerance. Sugar Tech 15: 116-121 https://doi.org/10.1007/s12355-013-0207-z
  21. Nakashima K, Fujita Y, Katsura K, Maruyama K, Narusaka Y, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2006) Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis. Plant Mol Biol 60:51-68 https://doi.org/10.1007/s11103-005-2418-5
  22. Nakano, T, Suzuki K, Fujimura T, Shinshi H (2006) Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol 140:411-432 https://doi.org/10.1104/pp.105.073783
  23. Office of the Cane and Sugar Board (2017) Report on Sugarcane Planting Areas in 2016/17 Planting Crop Year. 127p
  24. Passamani LZ, Barbosa RR, Reis RS, Heringer AS, Rangel PL, Santa-Catarina C, Grativol C, Veiga CFM, Souza-Filho GA, Silveira V (2017) Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots. PLOS ONE 12:1-21
  25. R Core Team (2013) R: A language and environment for statistical computing R. Foundation for Statistical Computing. Vienna, Austria. 409p
  26. Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi-Shinozaki K (2002) DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun 290:998-1009 https://doi.org/10.1006/bbrc.2001.6299
  27. Santana MJ, Carvalho JA, Souza KJ, Sousa AMG, Vasconcelos CL, Bastos Andrade LA (2007) Effects of irrigation water salinity upon the sprouting and initial development of sugarcane (Saccharum spp) and on soils with different textural levels. AgroTech J 31:1470-1476
  28. Shen YG, Zhang WK, He SJ, Zhang JS, Liu Q, Chen SY (2003) An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress. Theor Appl Genet 106:923-930 https://doi.org/10.1007/s00122-002-1131-x
  29. Silva EN, Ribeiro RV, Ferreira-Silva SL, Viegas RA, Silveira JAG (2010) Comparative effects of salinity and water stress on photosynthesis, water relations and growth of Jatropha curcas plants. J Arid Environ 74:1130-1137 https://doi.org/10.1016/j.jaridenv.2010.05.036
  30. Singh R, Jones T, Wai CM, Jifon J, Nagai C, Ming R, Yu Q (2018) Transcriptomic analysis of transgressive segregants revealed the central role of photosynthetic capacity and efficiency in biomass accumulation in sugarcane. Sci Rep 8: 1-10 https://doi.org/10.1038/s41598-017-17765-5
  31. Stockinger EJ, Gilmour SJ, Thomashow MF (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain containing transcriptional activator that binds to the C repeat/DRE, a cis acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci USA 94:1035-1040 https://doi.org/10.1073/pnas.94.3.1035
  32. Sun J, Peng X, Fan W, Tang M, Liua J, Shen S (2018) Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant. Broussonetia papyrifera. Gene 535:140-149 https://doi.org/10.1016/j.gene.2013.11.047
  33. Taiz L, Zeiger E (2013) Fisiologia Vegetal. 5a ed. Porto Alegre, ArtMed. 954p
  34. Vasantha S, Venkataramana S, Rao PNG, Gomathi R (2010) Long term salinity effect on growth, photosynthesis and osmotic characteristics in sugar cane. Sugar Tech 12:5-8 https://doi.org/10.1007/s12355-010-0002-z
  35. Wahid A, Rao A, Rasul E (1997) Identification of salt tolerance traits in sugarcane lines. Field Crops Res 54:9-17 https://doi.org/10.1016/S0378-4290(97)00038-5
  36. Wang J, Roe B, Macmil S, Yu Q, Murray JE, Tang H, Chen C, Najar F, Wiley G, Bowers J, van Sluys MA, Rokhsar DS, Hudson ME, Moose SP, Paterson AH, Ming R (2010) Microcollinearity between autopolyploid sugarcane and diploid sorghum genomes. BMC Genomics 11:261 https://doi.org/10.1186/1471-2164-11-261
  37. Weigel D (1995) The APETALA2 domain is related to a novel type of DNA binding domain. Plant Cell 7:388-389 https://doi.org/10.1105/tpc.7.4.388
  38. Willadino L, OliveiraFilho RA, Silva EA, GouveiaNeto AS, Camara TR (2011) Estresse salino em duas variedades de cana-de-acucar: enzimas do sistema antioxidativo e fluorescencia da clorofila. Revis Ciencia Agron 42:417-422 https://doi.org/10.1590/S1806-66902011000200022
  39. Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57:781-803 https://doi.org/10.1146/annurev.arplant.57.032905.105444
  40. Yang G, Yu L, Zhang K, Zhao Y, Guo Y, Gao C (2017) A ThDREB gene from Tamarix hispida improved the salt and drought tolerance of transgenic tobacco and T. hispida. Plant Physiol Biochem 113:187-197 https://doi.org/10.1016/j.plaphy.2017.02.007
  41. Yao W, Yaru F, Zhang Y, Li HE (2016) Cloning of four DREB genes from Tibetan Sophora moorcroftiana and analysis of their expression during abiotic stress. J Forest Res 27:675-683 https://doi.org/10.1007/s11676-015-0179-9
  42. Zhao J, Ren W, Zhi D, Wang L, Xia G (2007) Arabidopsis DREB1A/CBF3 bestowed transgenic tall fescue increased tolerance to drought stress. Plant Cell Rep 26:1521-1528 https://doi.org/10.1007/s00299-007-0362-3
  43. Zhang P, Yang P, Zhang Z, Han B, Wang W, Wang Y, Cao Y, Hu T (2014) Isolation and characterization of a buffalograss (Buchloe dactyloides) dehydration responsive element binding transcription factor, BdDREB2. Gene 536:123-128 https://doi.org/10.1016/j.gene.2013.11.060