• Title/Summary/Keyword: one stage culture

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An Analysis on the Change of Behavioral Affordance of the Livingroom in a Residence Depending on the Child's Developmental Stage - Focused on Double-Income Families with One Child Residing in Apartment Housing in Seoul - (자녀의 성장에 따른 주거 내 거실 공간의 행태지원성 변화 연구 - 서울시의 아파트에 거주하는 1자녀 맞벌이 가구를 중심으로 -)

  • Lee, Eun Joo;Chong, Kyong-Suk;Kang, Hye-Yoen
    • Journal of the Korean housing association
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    • v.27 no.6
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    • pp.9-18
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    • 2016
  • This research was aimed at finding clues of improving behavioral affordance of the residential environment by scrutinizing change of the residents' behavior in a livingroom depending on the child's development stage. 30 double-income families with one child were the subjects of depths interview, survey, observation of this research, who were residents of 3-bedroom apartment housing in Seoul. They presented obvious different behavioral patterns according to their child's development stage. Breeding and playing behavior were prior to the others when their child was in childhood, then relaxing with TV and PC behavior were getting superior when their child was in school period. It is important that residents perceive that the numerous types of behavior may take place in a livingroom, some of which has originated in our long residential culture. And then real housing should be provided to them that has proper behavioral affordance to support all those behavior. Housings with minimum walls and rooms would make it possible. A family has its inner dynamics can live a sustainable life in that housing as a home, that lasts along their whole life.

Effect of Cell Stage on Development of Mice Embryo After Cryopreservation and Thawing (생쥐초기배아 세포기가 동결보존 및 융해후 생존에 미치는 영향)

  • Han, Hyuck-Dong;Kim, Young-Dae;Shon, Sung-Wook;Kwon, Jang-Yeon;Lee, Young-Jin;Chung, In-Bae;Cha, Dong-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.20 no.2
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    • pp.161-164
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    • 1993
  • To forsee appropriate developmental cell stage in human embryos for cryopreservation, we observed blastocyst development in culture medium after cryopreservatlon and thawing of one cell, two cell, four cell stage of mice embryos. According to our results, development of the blastocyst of cryopreserved two cell mice embryos was significantly higher than that of cryopreserved one cell mice zygotes or four cell mice embryos.

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Development of Continuous Culture Process for Economic Production of Hyaluronic Acid (HA) Biosynthesized by Streptococcus zooepidemicus (Streptococcus zooepidemicus 유래 히알루론산의 경제적 생산을 위한 연속배양 공정 개발)

  • Kim, Soo Yeon;Chun, Gie-Taek
    • Microbiology and Biotechnology Letters
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    • v.48 no.4
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    • pp.525-532
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    • 2020
  • A continuous fermentation process was carried out to enhance hyaluronic acid (HA) production using Streptococcus zooepidemicus cells. During the 1st stage continuous operation from 8 h with a dilution rate of 0.029/h (D1), HA was produced in the range of 7.5-10 g/l. During the 2nd stage from 44 h with a dilution rate of 0.036/h (D2), HA production (8.28 g/l) was initially reduced to a small extent due to increase of dilution rate from D1 to D2, and then a new pseudo-steady state was formed within a few hours with a concurrent small variations of HA production. The HA amount produced during the latter part of the 2nd stage was stably maintained in the range of 8.28-9.48 g/l, about 4.7% less amount compared to the 1st stage. Due to 24% increase of dilution rate from D1 to D2, however, maximum volumetric productivity (DP) amounting to 0.341 g/l/h was obtained at 96 h during the 2nd stage. This maximum productivity obtained from the continuous culture turned out only a small increase (3%) as compared to the corresponding batch fermentation. However, it should be noted that, in the case of batch process, one run typically consists of serial stages of growth culture plus one final production culture. This implies that, if the continuous fermentation that practically needs no dead time necessary for the multi-stage growth cultures is run for longer period, the total amount of the accumulated HA would be far greater than the amount obtained from the corresponding batch culture performed for the identical period.

A Study on Postmodernism Style of Fashion - Concentrating on the 1996-2000- (현대 복식에 나타난 포스트모더니즘 양식 연구 -90년대 후반 복식을 중심으로-)

  • Kim Hee-Kyun;Chung Hei-Jung
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.3_4 s.141
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    • pp.569-575
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    • 2005
  • The objective of this study is to consider the concept of postmodernism and the characteristics concerning the stage of the development, and to investigate the effect of the postmodernism on the clothing. Postmodernism is the result of the late capitalism and its characteristics are the narrative, mixture and deconstruction. First, since it is impossible to express the social culture of various society through one theoretical disclosure, the novel value on the narrative is provided and its characteristics arise. Hence, the society becomes afford to accept the other and provides the novel value to the absolute sub-concept. Second, the recognization and emphasis of the narrative in the post modem society bring about the repulsion as to the strict classification between the high culture and low one established from modernism and the closure between each genre of the art, and this causes to raise the composed characteristics which borrow or mix the style and image of different age and culture. Third, the deconstructive feature arises which eliminates the boundary between the high and low class, the past and future and the destruction and construction. This means not only anther recomposition but also skeptical attitude about it.

Replication and Sequential Development of Adherent Mycoplasma Pneumoniae Studied by Light and Electron Microscopies (광학(光學) 및 전자현미경기술(電子顯微鏡技術)에 의(依)한 Mycoplasma pneumoniae의 분열(分裂)과 연속분화(連續分化)에 관(關)한 연구(硏究))

  • Kim, C.K.;Pfister, Robert M.
    • Applied Microscopy
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    • v.12 no.2
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    • pp.11-22
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    • 1982
  • The morphological development of Mycoplasma pneumoniae attached to solid surfaces was examined by light and electron microscopies. Critical point drying and carbon replication techniques revealed that during the growth cycle of developing microcolonies, the morphological form coincided with the pH of the culture. M. pneumoniae appeared to have a well defined morphology associated with age of the culture. The organisms were dimorphic, with round cells capable of reproduction and segments consisting of a spindle shaped body with one pointed and one knob-like end. Starting with single cells, there were the following stages in the development of a culture: replication stage through binary fission and segmentation, stage of confluency, and a degeneration stage into rough spherical forms. The round cells appearrd to replicate by binary fission during the lag and early log phases of growth, while spindle segments replicated by segmentation during most of the logarithmic growth. The growth of the filaments and replication of the segments occured at the knob-like ends, showing a type of polarity, and formed a meshwork across the surface. This development could be cycled under favorable growth conditions, but the culture aged and when the conditions became adverse(e.g. pH 6.8 or lower), filamentous cells converted to spherical forms, losing their reproductive capability.

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In vitro Fertilization and Culture of Rabbit Egg (가토난자의 체외수정과 체외배양)

  • 박흠대;이경광;정길생
    • Korean Journal of Animal Reproduction
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    • v.4 no.1
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    • pp.75-82
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    • 1980
  • This experiment was carried out to improve a simple and effective procedure of egg transfer which is considered to be the most useful technique for the improvementand proliferation of domestic animals. Several experiment procedures such as superovulation, surgical recovery of ovulated egg, in vitro capacitation of ejaculated spermatozoa, in vitro fertilization and culture of embryo were conducted. The results obtained in this experiment were summarized as follows: 1. In rabbits treated with PMS in combination with estradiol and HCG, 10 to 43 eggs were obtained in one rabbit and the average ovulation number of 20 rabbits was 21 eggs. 2. Six to 24 eggs (average 13 eggs) were recovered from the removed oviducts by the methods of flushing technique and the average recovery rate of 20 rabbits was 61.9 percent. 3. Most rabbit spermatozoa ejaculated by artificial vagina were capacitated in vitro by the culture of the spermatozoa with PBI medium at 37$^{\circ}C$ for 4 hours after removal of seminal plasma. 4. Normal fetilization following in vitro fetilization was observed in 88 (42.7%) of 206 eggs. 5. The number of eggs developed to 2-, 4-, and 8-cell stage following in vitro culture with PBI medium at 37$^{\circ}C$ was 26 (70.3%), 20 (54.1%) and 17 (45.9%) of 37 eggs used for in vitro culture.

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A study on the process of spatial reduction of cotton culture in Korea since 1945 (해방 이후 우리나라 면작농업 소멸의 지역적 전개과정)

  • ;Kim, Kihyuk
    • Journal of the Korean Geographical Society
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    • v.29 no.3
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    • pp.318-339
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    • 1994
  • U.S. had given large amount of cotton to Korea as food aid program since 1945. This cotton aid had negative impact on cotton culture in Korean agriculture. Korean government used counterparts funds (sale proceeds of food aid) not for investment to agriculture sector, but for military budgets. And food aid on program type had influenced general economic policies, which neglected agricultural sector too. Anti-agricultural policy which was helped by U.S. food aid, had caused cotton cultivator an economic loss. So this economic loss had made many farmers abandon cotton culture. But in our times, cotton is cultivated for the purpose of domestic consumption in a few rural villages. The purposes of this study are 1) to analyze the process of spatial reduction of cotton culture since 1945 in regional contexts in Korea, and 2) to identify the function and meaning of cotton culture which does not pay off in agricultural region. Materials for acreage of cotton culture are acquired through the agricultural statistical year book(1952-1989) and census. To clarify the meanings of cotton culture, field survey are conducted in a rural village which is identified as only one where cotton was cultivated in 1993. In these contexts, this study has come to the following conclusions. In the period of under the rule of Japanese Imperialism (1910-1945), G. arboreum, species of cotton which was traditionally cultivated since 1364, had been driven out. And G. hirustun species, which is suitable for the production of highly qualified textile, has been hierarchically diffused by policy. In these period, regional structure of Korean agriculture was reorganized for the provision with food to Japan. Crops leading this dependent spatial structure were rice and cotton. So agricultural region, specialized with cotton, were distributed in the hinterland of the area which is specialized with rice. U.S. cotton aid to Korea began in 1947. U.S. took an interest in agricultural export because of her domestic surplus of cotton. Cotton aid is one mechanism by which U.S government developed agricultural market in recipient countries, Specially in the exchange rates, up-valuation of won to the U.S. dollars made domestic cotton more expensive than cotton imported, Production cost of domestic cotton is higher than Government's purchasing price of cotton which was also more expensive than price of cotton imported. Korean farmer could not help abandoning the cultivation of cotton, and this gave rise to spatial reduction of cotton culture. Spatially, cotton culture was abandoned in early stage of reduction in regions where stand at a disadvantage climatically, and in next stage in regions where other up-land crops which paid off in urban market, eg, fruits, could be cultivated. In the stage of extinction, cotton was cultivated only in area where G. hirustun species was originated in Korean peninsula. This region is not only suitable climatically for cotton culture, but is far away from urban market. Use of cotton produced is not for spinning, but for fillings of comforter. The main purpose of cotton culture in rural village is not for cotton yields, but for increase of production of seasame, which is grown together with cotton as mixed crops. Cotton product are used for domestic consumption and sold out to gin house. Though cotton culture is not paid off, farmer wanted to cultivate continuously for the cultural purpose, and they wanted the cotton culture promotion policy with the goverment subsidy.

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Mass Production of Calla Lily(Zantedeschia spp. Southern Light) by the Immature Zygotic Embryo Culture (유색칼라(Zantedeschia spp. Southern Light) 미숙배 배양에 의한 다량증식)

  • 고정애;최소라;김현순
    • Korean Journal of Plant Resources
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    • v.16 no.2
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    • pp.160-167
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    • 2003
  • In order to investigate the effects of developmental stage of embryos and plant growth regulators on mass production of Zantedeschia spp. Southern Light, immature zygotic embryos of Zantedeschia spp. Southern Light were cultured on Murashige and Skoog(1962) basal media or containing 2,4-D, NAA and BA. Globular embryos did not grow on any of the 2,4-D, NAA and BA combinations. The most suitable stage of immature zygotic embryo culture on the induction callus and multiple shoot was at early cotyledonary embryo stage, and at this stage of embryos were germinated up to 87.5%. The whitish watery callus and yellowish compact nodular callus produced on all 2,4-D, NAA and BA media. The best combination for inducing embryogenic callus was 0.5 mgL NAA and 1.0 mg/L BA. Whitish watery calli have been subcultured for more than 8 months and have retained their producing ability, Plant regeneration was only obtained by direct shoot development and yellowish compact nodular calli. Abundant plantlets were regenerated from cotyledonary stage of embryo culture on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA. Supplementation of the media with 10% coconut water showed as the best concentration for plant differentiation from direct developed of shoots. The number of regenerated plants from one embryo could be seperated 25-35s plantlets. All yellowish compact callus-derived plantlets were transferred to pots containing a mixture of vermiculite, perlite and sand(1:1;1 v/v) and 100% of divided plantlets were phenotypically normal.

The Development of a Computer-Assisted HACCP Program for the Microbiological Quality Assurance in Hospital Foodservice Operations (병원급식의 미생물적 품질보증을 위한 HACCP 전산프로그램의 개발)

  • Kwak, Tong-Kyung;Ryu, Kyung;Choi, Seong-Kyung
    • Journal of the Korean Society of Food Culture
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    • v.11 no.1
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    • pp.107-121
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    • 1996
  • This study was carried out to develop the computer-assisted Hazard Analysis and Critical Control Point (HACCP) program for a systematic approach to the identification, assessment and control of hazards for foodservice manager to assure the microbiological quality of food in hospital foodservice operations. Sanitation practices were surveyed and analyzed in the dietetic department of 4 hospitals. Among them, one 762-bed general hospital was selected as standard model to develop computer-assisted HACCP program. All data base files and processing programs were created by using Foxpro package for easy access of HACCP concept. HACCP program was developed based on the methods suggested by NACMCF, IAMFES and Bryan. This program consisted of two parts: the pre-stage for HACCP study and the implementation stage of the HACCP system. 1. Pre-stage for HACCP study includes the selection of menu item, the development of the HACCP recipe, the construction of a product flow diagram, and printing the HACCP recipe and a product flow diagram. A menu item for HACCP study can be selected from the menu item lists classified by cooking methods. HACCP recipe includes ingredients, their amount and cooking procedure. A flow diagram is constructed based on the HACCP recipe. The HACCP recipe and a product flow diagram are printed out. 2. Implementation of HACCP study includes the identification of microbiological hazards, the determination of critical control points, the establishment of control methods of each hazard, and the complementation of data base file. Potentially hazardous ingredients are determined and microbiological hazards are identified in each phase of the product flow. Critical control points (CCPs) are identified by applying CCP decision trees for ingredients and each process stage. After hazards and CCPs are identified, criteria, monitoring system, corrective action plan, record-keeping system and verification methods are established. When the HACCP study is complemented, HACCP study result forms are printed out. HACCP data base file can be either added, corrected or deleted.

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User-friendly adjustable table fan with selective rotation angles (사용 편의성 향상을 위한 선풍기의 효율적 회전구간 선정)

  • Kim, Sang-Hyun
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.2
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    • pp.53-58
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    • 2022
  • Since a general household fan has only one left/right turning stage, the rotation angle cannot be adjusted leading to cases whether the wind reaches to an unnecessary area or vice versa. In this paper, we propose a method to efficiently control the turning section to selectively send wind to a necessary space while reducing energy waste. The minimum rotation angle was obtained by experimentally measured the stationary wind direction angle of the fan, and the optimal number of turning stages was selected by appropriately dividing the space where the wind reaches. Through this, it was confirmed that if the fan has a minimum rotation angle of 45°, a turning section of 3 stages and its rotation angle is increased by twice the stationary wind direction angle at each stage, the wind is distributed efficiently. Therefore, it is considered that the selective turning stage control proposed in this paper can minimize energy waste without significant change of the fan structure.