• KSBB Journal
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• v.13 no.2
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• pp.181-186
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• 1998

In order to obtain high-level production of recombinant human thrombopoietin (rhTPO) in insect cell line, HTI-TN-5B1-4 (TN5), conditions for optimal rhTPO expression such as multiplicity of infection (MOI), the cell density at infection, harvesting time and type of culture method as well as growth media were determined. When TN5 cells were cultured as anchorage-dependent state in 60-mm dish, cell density $2\times^6$ cells,MOI of 10 and Garvesting the culture media at 72 hr post-infection wrere the cinditions for highest rh TPO production. High production of rhTPO was also achieved by using EXPRESS FIVE serum free media rather than SF900II serum free media-1. Anchorage-dependent TN5 cells were adapted as a suspension culture when they were grown in the presence of heparin. TN5 cells were successfully cultured at 0.2 L scale in suspension culture without having aggregation. When TN5 cells were cultured as suspension state, cell density of $0.6\times10^6$ cells/mL, MOI of 1 and harvesting the culture media at 72 hr post-infection, gave the highest yield of rhTPO.

• Parasites, Hosts and Diseases
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• v.26 no.2
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• pp.95-106
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• 1988

Specific or non-specific cytolytic processes of free-living amoebae causing meningoencephalitls have been emphasized and the cytolytic ability related to hydrolases in Entantoeba sp. and Naegleria sp. has also been reported since the latter half of 1970's. However, no information on hydrolase activities in Acanthamoeba sp. is available. Hydrolases in Acanthamoeba culbertsoni, a pathogenic species of free-living amoebae, were assayed and compared with those in a non-pathogenic species, A. royreba. Pathogenicity of these two species was confirmed through experimental infection to BALB/c mice. Hydrolase activities and cytotoxic effects between pathogenic and non.pathogenic species were compared in the trophozoites cultured in CGV media and in CHO cell line, respectively. The results are summarized as follows: 1. The mice infected with A. culbertseni were all dead 15 days after nasal inoculation, and the mean survival time was 8.5 days. Also the mice infected with this pathogenic species manifested typical meningoencephalitis, whereas the mice infected with A. royreba did not. 2. Hydrolases detected both in the cell extracts and culture media were acid phosphatase, ${\beta}-N-acetyl$ galactosaminidase, ${\beta}-N-acetyl$ glucosaminidase, ${\alpha}-mannosidase$, neutral proteinase and acid proteinase, all of which were detected with remarkably higher rate in A. culbertsoni than in A. royreba. 3. A. cuzbertsoni revealed strong cytotoxicity for the target CHO cells, whereas A. royreba did not show any specific cytotoxicity. About 80% of the target cells mixed with A. culbertsoni were dead 48 hours after cultivation, and more than 95% of the target cells were dead 72 hours after cultivation. 4. Hydrolase activities in A. culbertsoni cultured with the target cell line were assayed according to the culture time. The activities of acid phosphatase, ${\beta}-N-acetyl$ galactosaminidase, ${\beta}-N-acetyl$ glucosaminidase, ${\alpha}-mannosidase$ and acid proteinase in this pathogenic amoeba were detected higher in amoeba extracts than in culture media up to 120 hours after cultivation, but after 120 hours of cultivation those activities were detected higher in culture media than in the amoeba Iysates. Neutral proteinase activity in A. culbertsoni increased more in EBSS medium than in the Iysate specimens although the activity in the extracts was generally steady according to the cultivation time. Summarizing the above results, it is concluded that there were differences in hydrolase activities between Pathogenic A. culbertsoni and non-pathogenic A. royreba, and that some hydrolase activities were detected remarkably higher in A. culbertsoni which revealed strong cytotoxicity to the target CHO cell line.

• Journal of the Korea Convergence Society
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• v.12 no.7
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• pp.133-142
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• 2021

This study aims to expand the effect of online education into the aspect of active exploration and sharing of class-related issues by learners. Based on theoretical discussions, Two types of motivation (personal and social) to explore issues, engagement, attitude toward issue content, and eWOM model were verified. As a result of the study, it was found that the impact of personal and social motivations that online education has on engagement on specific issues, and the positive(+) influence on attitudes toward issue content and word of mouth intentions on SNS, considering engagement as a parameter. In this study, the role of engagement in inducing the next learning by oneself was confirmed, and it can be seen that social and personal motives for issues and class content should be utilized to increase engagement.

• The Transactions of the Korea Information Processing Society
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• v.4 no.9
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• pp.2378-2390
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• 1997

We, in this paper, have implemented E1 HDSL(high-bit-rate digital subscriber line) function over an ATM switching system. The maximum loop lengths for subscriber service and cell loss rates to meet the bit error rate of $10^{-7}$ at transmission of 2B1Q HDSL data of E1 rate over existing telephone copper wires in the presense of the significant impairments such as crosstalk, impulse noise, power line noise and longitudinal over the CSAs environment consisting of 26 gauge and 24 gauge unloaded copper telephone lines has assessed. We have confirmed the typical media services function such as video on demand(VOD) service for MPEG-1, image conference service and high-speed Internet access service over ATM switching system.

• Journal of The Korean Astronomical Society
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• v.48 no.3
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• pp.195-202
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• 2015

Star forming galaxies found in the early universe exhibit asymmetric Lyα emission line that results from multiple scattering in a neutral thick medium surrounding the Lyα emission source. It is expected that emergent Lyα will be significantly polarized through a large number of resonance scattering events followed by a number of successive wing scatterings. In this study we adopt a Monte Carlo method to calculate the polarization of Lyα transferred in a very thick static slab of H_I. Resonantly scattered radiation associated with transitions between is only weakly polarized and therefore linear polarization of the emergent Lyα is mainly dependent on the number of off-resonant wing scattering events. The number of wing scattering events just before escape from the slab is determined by the product of the Doppler parameter a and the line center optical depth τ₀, which, in turn, determines the behavior of the linear polarization of Lyα. This result is analogous to the study of polarized radiative transfer of Thomson scattered photons in an electron slab, where the emergent photons are polarized in the direction perpendicular to the slab when the scattering optical depth is small and polarized in the parallel direction when the slab is optically thick. Our simulated spectropolarimetry of Lyα shows that the line center is negligibly polarized, the near wing parts polarized in the direction parallel to the slab and the far wing parts are polarized in the direction perpendicular to the slab. We emphasize that the flip of polarization direction in the wing parts of Lyα naturally reflects the diffusive nature of the Lyα transfer process in thick neutral media.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.05a
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• pp.420-424
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• 2016

Recently, with the development of Internet technology and multi-media technology, the Internet is going to develop in many areas, a new application areas. In particular, in the area of education, has made a Epoch-making development in the Internet applications, it has presented the instructional methods of the new paradigm. Study using the online learning, instructional method of a conventional traditional new proposal that deviates from the off-line teaching, Unlike the existing off-line learning, without being bound by time and space, in terms of anytime, anywhere it is possible to attend the lecture, is a very efficient learning. Online lectures Despite many advantages, and containing a number of problems. In terms of space of the learning is performed on-line, there is a disadvantage that the student management and learning, the reliability of evaluation missing number. In this study, out of such a variety of problems, concentration to induce an active learning attitude of learners, learners of learning who attempt to increase the reliability and using the face detection system of attendance learning It proposed a degree system.

• Journal of Digital Convergence
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• v.19 no.3
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• pp.177-185
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• 2021

This paper aimed to grasp what the percentage in the types of news stories on the online broadcast is, which was conducted by analyzing the news stories of 9 broadcasting websites on the Naver newsstand. For the analysis, a total of 270 days' samples were selected, including 30 days per broadcast on 9 broadcasting websites. For a method of analysis, One-way ANOVA was used to examine the difference among broadcasting websites. The analysis was made centering with priorities given to the type of news stories by the composition of language, the type of genre as a standard of stories, and so on. As a result of analysis, all the programs in the off-line broadcast have been produced and transmitted as a video-typed story, but a half of those in on-line broadcast have been made up of the stories composed of photo and text. The online newspaper has been producing a new type of news' story using video-typed story or computer graphic while the online broadcast has actively been utilizing stories composed of photos and text, which are types of newspaper's stories. From above-mentioned results, it can be understood that the boundary among media is getting more and more indistinct on the environment of online media, showing the phenomenon that the type of broadcast's stories is becoming old-fashioned.

• Journal of the Korea Convergence Society
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• v.8 no.11
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• pp.233-240
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• 2017

In recent years, graphics authoring tools have evolved into a variety of formats in line with the rapidly changing digital design field. However, in the field of interface design, screen shape and size depending on the user's environment, it needs more flexible and scalable authoring tools. Along with the emergence of variety of new tools, work processes are also changing. This study investigates what kinds of graphic authoring tools are currently used by designers and compared the result to the case of overseas and examined the difference in the environment of graphic authoring tool. This study looked at criteria and satisfaction of designers choosing authoring tools and how the choice depends on the type of company we are working in and the type of project we are involved in. Research shows that the choice of authoring tools depends on the type and efficiency of the project. Based on this, a reference is provided to establish the standard and working environment for designers and companies to select graphic authoring tools and propose the future direction of graphic authoring tool environments.

• Journal of the Korea Convergence Society
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• v.11 no.8
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• pp.183-194
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• 2020

Recently, in Korea, financial institutions such as banks are most severely affected by the universalization of the Internet and smartphones. On the other hand, the use of online systems by financial institutions keeps increasing; the convenience of online services has a significant influence on the attraction and the retention of financial customers; consumer needs are also diversely expressed. This paper deduces from the precedent researches a mechanism that online financial system enhances the trust of customers -the medium of the online system and other customers- and its perceived easiness affects its perceived effectiveness and then all these internal variables induce satisfaction. Plus, this paper aims at verification of the hypothesis in terms of an extended technology acceptance model, based on the hypothesis that word of mouth and repurchase are significantly linked to this mechanism. Through this study, the researchers tried to check how the online service quality and emotional factors of financial institutions affect the users in accordance with the trend of changes in the service usage method of financial institutions, and confirmed that the hypothesis was not rejected.

• Journal of the Korea Convergence Society
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• v.8 no.8
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• pp.231-237
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• 2017

The purpose of this study is to investigate the need for smart home service based on the lifestyle of a rapidly growing single household. When we compare the need and the arbitrary service according to the pattern of the lifestyle of the consumer, we try to analyze whether there is an effect on the future use. In the questionnaire survey of 1 person, 8 questions were selected such as food, state, line, leisure, communication, consumption, mobile life, Single household characterization question should be added so that concrete measures can be presented to future services. Competitive smart home services will be presented by examining the lifestyle of the 20th and 30th generation of single households, It is intended to be used as basic data on the direction of development of smart home service of the rapidly increasing single person households, and it is aimed to be a direction of improvement and development direction of the existing plan.