• The Journal of the Korea Contents Association
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• v.17 no.9
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• pp.184-191
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• 2017

Smartphone has become a part of many people's daily lives. The mobile messenger function on the smartphone in particular is the most frequently used services. The mobile communication tool in the age of digital media can be classified into two big categories: text messages and emoticons, the non-verbal expression tool of one's emotions. This thesis has studied the mobile communications made by the non-verbal tool of emoticons. The history of emoticons traces back to the text-based emoticon created by Scott Fahlman, from which graphical emoticons were developed and the sound and animation effects have been added. Many people use emoticons and diverse companies are developing the tool. In this study, the representative emoticon development case of Line Co., Ltd. is analyzed to see the concept, types, development process, collaborations and merchandising of the emoticons. Based on the case of Line, the development orientation and direction of domestic emoticons will be discussed to be culture contents.

• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.4 no.1
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• pp.11-19
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• 2008

Groundwater flow in confined aquifer and heat transport in underground geologic media are using same governing equation(line source) like well fuction. Therefore the conventional slope method using only later data obtained from in-situ thermal response test to determine the thermal conductivity of vertical geothermal heat exchanger(GHEX) is basically identical with one of Theis straight line method of aquifer test under artesian condition. In case that the pumping rate(Q, $m^3$/d) and drawdown(s,m) which are used for input data of existing hydrogeologic computer programs for aquifer test are replaced and converted to supplying heat energy per unit length of bore hole(Q/L,w/m or Kcal/h.m) and temperatures (T,$^{\circ}C$)measured at in and out-let of GHEX as in put data respectively, thermal conductivity around geothermal heat exchanger can be easily estimated without any special modification of the existing hydrogeologic computer program. Two numbers of time series temperature variation data obtained from in situ geothermal response test are analized using Theismethods(standard curve and straight line method) by using existing aquifer test program and conventional Slope method proposed by ASHRAE. The results show that thermal conductivity values estimated by two straight methods are identical and the difference of estimated values between standard curve methods and Slope method are also within acceptable ranges. In general,the thermal conductivity estimated from Theis straight linemethod gives more accurate value than the one of Slope method due to that Slope method uses only visual matching otherwise Theis method uses automatic curve matching estimation with reducing RSS.

• Journal of Plant Biotechnology
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• v.49 no.4
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• pp.331-338
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• 2022

Sunflower (Helianthus annuus L.) protoplasts were isolated from seven-day-old etiolated hypocotyls of 10 A line and four-week-old fully expanded young leaves of PI 441983 line in vitro seedlings using an enzymatic method. Purified protoplasts were collected by filtration and floatation in sucrose solution. Semi-solid protoplast culture was performed using the L4 regeneration protocol with various culture media and plating densities to achieve the highest efficiencies for protoplast culture of hypocotyl and mesophyll protoplasts of 10 A and PI 441983 lines, respectively. The concentrations in liquid L'4M medium and different plating densities were evaluated in two types of cytokinins, the adenine-type 6-benzyladenine (BA) and the phenylurea-type thidiazuron (TDZ). The highest colony formation was achieved in both sunflower lines when 0.5 mgL^-1 BA and 0.5 mgL^-1 TDZ were applied with a high plating density (3 × 10⁵ protoplasts mL^-1). These conditions led to 38.45% and 39.40% colony formation for hypocotyl protoplasts of the 10 A line and mesophyll protoplasts of the PI 441983 line, respectively. Moreover, many hypocotyl protoplast-derived colonies developed into micro-calli. In addition, superior development of both sunflower protoplasts was observed with all plating densities when BA was used in combination with TDZ. This finding will be applicable to future sunflower hybrid production via somatic hybridization.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.143-146
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• 1989

In order to clarify the characteristics of anthocyanln-producing cell line selected by in vitro grown cultures of ginseng (Panax ginseng C.A. Meyer), the calli of anthocyanin-Producing strain were cultured on media supplemented with different concentrations of 2,4-D and sucrose under light or dark condition. The light was found to be essential for anthocyanin synthesis. Anthocyanin synthesis of ginseng cell line was inhibited by the increase of 2,4-D in the medium. On the other hand, sucrose promoted the anthocyanin-production and the optimum concentration of sucrose for the highest production of anthocyanin was 5%. The growth of anthocyanin-producing tell line was best on the medium supplemented with 0.5 mg/l 2,4-D and 5% sucrose.

• Journal of Broadcast Engineering
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• v.21 no.6
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• pp.977-985
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• 2016

In this paper, we propose a new method for on-line genre classification using spectrogram and deep neural network. For on-line processing, the proposed method inputs an audio signal for a time period of 1sec and classifies its genre among 3 genres of speech, music, and effect. In order to provide the generality of processing, it uses the spectrogram as a feature vector, instead of MFCC which has been widely used for audio analysis. We measure the performance of genre classification using real TV audio signals, and confirm that the proposed method has better performance than the conventional method for all genres. In particular, it decreases the rate of classification error between music and effect, which often occurs in the conventional method.

• Journal of Korea Multimedia Society
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• v.6 no.3
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• pp.527-538
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• 2003

The contents of remote lectures are written by authoring tools or animation tools. Written contents then integrate existing medias, and are stored in a file type, which is added to the lecturing activities of lecturers. These files are stored according to the kinds of each authoring tool. So, there is no interchangeability and the efficiency of each media is not so good. Due to these structural features, it is not possible to do streaming lectures in on-line environments. In this study, a technique was suggested, which guarantees the interchangeability of remote lecturing contents using SMIL which is a synchronizing multimedia language. The suggested technique is an extended type of SMIL, and includes tags to represent the lectures of tutors which are the specific feature of remote lectures. Additionally, a model, which does streaming SMIL remote lecture contents through on-line, and which transfers control techniques are suggested. The remote lecture contents established by the proposed method can be applied to all authoring tools for remote lectures just by installing conversion modules, and can be a kind of expression model of the synchronizing model for streaming service in on-line environment.

• Journal of Korean Neurosurgical Society
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• v.49 no.1
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• pp.13-19
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• 2011

Objective: The purpose of this study is to investigate the combined effects of ginkgo biloba extract, ginkgolide A and B and aspirin on SK-N-MC, human neuroblastoma cell viability and mRNA expression of growth associated protein43 (GAP43), Microtubule-associated protein 2 (MAP2), B-cell lymphoma2 (Bcl2) and protein53 (p53) gene in hypoxia and reperfusion condition. Methods: SK-N-MC cells were cultured with Dulbecco's Modified Eagle's Medium (DMEM) media in $37^{\circ}C$, 5% $CO_2$ incubator. The cells were cultured for 8 hours in non-glucose media and hypoxic condition and for 12 hours in normal media and $O_2$ concentration. Cell survival rate was measured with Cell Counting Kit-8 (CCK-8) reagent assay. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate mRNA levels of GAP43, MAP2, Bcl2, and p53 genes. Results: The ginkgolide A and B increased viable cell number decreased in hypoxic and reperfused condition. The co-treatment of ginkgolide B with aspirin also increased the number of viable cells, however, there was no additive effect. Although there was no increase of mRNA expression of GAP43, MAP2, and Bcl2 in SK-N-MC cells with individual treatment of ginkgolide A, B or aspirin in hypoxic and reperfused condition, the co-treatment of ginkgolide A or B with aspirin significantly increased GAP43 and Bcl2 mRNA levels. In MAP2, only the co-treatment of ginkgolide A and aspirin showed increasing effect. The mRNA expression of p53 had no change in all treating conditions. Conclusion: This study suggests that the combined treatments of Ginkgo biloba extracts and aspirin increase the regeneration of neuroblastoma cells injured by hypoxia and reperfusion.

• Journal of Digital Contents Society
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• v.16 no.2
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• pp.189-196
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• 2015

The proposed cooperative 3D modelling system enables more efficient modeling works by improving current cooperation systems. Current 3D modeling tasks work on off-line and most of works are cooperation systems which communicate by files. These cooperation system cannot be interactive, occurs time delay while feedback and must have some programs which can read the files. This paper solves these problems using web that has the characteristics of network and proposes new cooperation system form. Using WebGL(Web Graphics Library) which is proposing technology in technical realization of Web 3D modeling, we make 3D objects in web browser and these objects can be manipulated by server-client communication. Therefore, if people use this system, they can use real time interaction and feedback. Also this system lowers a software installation necessity and can access everytime if web browser is installed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.1
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• pp.61-68
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• 2004

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains $5\textrm{mgL}^{-1}$ kinetin, $0.5\textrm{mgL}^{-1}$ NAA, 2 % sucrose (or maltose), 3% sorbitol, and $500\textrm{mgL}^{-1}$ proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• Journal of Internet Computing and Services
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• v.18 no.5
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• pp.95-102
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• 2017

With the development and popularization of Internet, the competition of SNS market is now getting fiercer and fiercer. SNS service providers need to keep updating their service to sustain their customers' continuance use. However, due to the homogenization of functions and services from different applications provided by different developers, there comes a new phenomenon that users shift from one SNS to another SNS. In line with this, the current study aims to explore factors affecting switching intention among SNS users by applying migration theory which explains people's migration behavior from one place to another. Findings from surveys of Korean and Chinese college students suggest that there was a difference of factors influencing switching intention among the two countries. For Korean college students, alternative attraction was the strongest factor leading to SNS switching intention. On the other hand, peer pressure was the strongest factor leading to SNS switching intention among Chinese college students.