• 제목/요약/키워드: on-line laboratory

검색결과 953건 처리시간 0.029초

The Study of Mutation Spectrum in Iac / Gene of Transgenic Big Blue$\textregistered$ Cell Line Following Short-Term Exposure to 4-Nitroquinoline N-oxide

  • Youn, Ji-Youn;Kim, Kyung-Ran;Cho, Kyung-Hea;Ryu, Jae-Chun
    • 한국환경독성학회:학술대회논문집
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    • 한국환경독성학회 1996년도 제19회정기학술대회(The 19th Symposium of the Korean Society of Environmental Toxicology)
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    • pp.64-64
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    • 1996
  • Transgenic animal and cell line models which are recently developed in toxicology field combined with molecular biological technique, are powerful tools for studying of mutation in vivo and in vitro, respectively. The Big Blue mutagenesis assay system is one of the most widely used transgenic systems. Especially, for the study of direct acting mutagens, Big Blue cell line is very useful and powerful to evaluate mutagenicity because the mutation frequency and mutationspectrlun showed no distinct differences between cell line and animal. The Big Blue cell lines carry stably integrated copies of lambda shuttle vector containing lac I gene as a mutational target. These lambda shuttle vectors are useful for various mutagenesis related studies in eukaryotic system due to their ability to be exposed mutagen and then transfer a suitable target DNA sequence to it convenient organism for analysis. We tried to assess the mutagenic effect of 4-NQO with Big Blue cell line. After the treatment of 4-NQO, genomic DNA was isolated and lambda shuttle vector was packaged by in Vitro packaging and then these were plated on bacterial host in the presence of X-gal to screen mutation in the lac I. We determined MF as a ratio of blue plaques versus colorless plaques and now undergoing the mutation spectrum of 4-NQO in lac J gene sequence.

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Nondestructive sensing technologies for food safety

  • Kim, M.S.;Chao, K.;Chan, D.E.;Jun, W.;Lee, K.;Kang, S.;Yang, C.C.;Lefcourt, A.M.
    • 한국환경농학회:학술대회논문집
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    • 한국환경농학회 2009년도 정기총회 및 국제심포지엄
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    • pp.119-126
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    • 2009
  • In recent years, research at the Environmental Microbial and Food Safety Laboratory (EMFSL), Agricultural Research Service (ARS) has focused on the development of novel image-based sensing technologies to address agro-food safety concerns, and transformation of these novel technologies into practical instrumentation for industrial implementations. The line-scan-based hyperspectral imaging techniques have often served as a research tool to develop rapid multispectral methods based on only a few spectral bands for rapid online applications. We developed a newer line-scan hyperspectral imaging platform for high-speed inspection on high-throughput processing lines, capable of simultaneous multiple inspection algorithms for different agro-food safety problems such as poultry carcass inspection for wholesomeness and apple inspection for fecal contamination and defect detection. In addition, portable imaging devices were developed for in situ identification of contamination sites and for use by agrofood producer and processor operations for cleaning and sanitation inspection of food processing surfaces. The aim of this presentation is to illustrate recent advances in the above agro.food safety sensing technologies.

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모델급 TCSC의 설계 및 구현 (Design and Implementation of a Laboratory Scale TCSC)

  • 딘민차우;박상민;김성규;박민원;유인근
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2015년도 제46회 하계학술대회
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    • pp.351-352
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    • 2015
  • Thyristor-controlled series capacitor (TCSC) is a power electronic-based device that provides a fast and controllable series compensation of transmission line reactance. To match with laboratory facilities and for further research initiatives, a practical laboratory scale TCSC was designed and fabricated in this paper. The TCSC parameters were designed based on the terminologies such as percentage of compensation, boost factor and resonance factor. According to the design parameters, a prototype laboratory scale TCSC with a constant reactance controller was fabricated and tested. The measured results from the laboratory scale TCSC demonstrate the ability of the TCSC to provide rapid control of series reactance of a transmission line.

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Locally Initiating Line-Based Object Association in Large Scale Multiple Cameras Environment

  • Cho, Shung-Han;Nam, Yun-Young;Hong, Sang-Jin;Cho, We-Duke
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제4권3호
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    • pp.358-379
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    • 2010
  • Multiple object association is an important capability in visual surveillance system with multiple cameras. In this paper, we introduce locally initiating line-based object association with the parallel projection camera model, which can be applicable to the situation without the common (ground) plane. The parallel projection camera model supports the camera movement (i.e. panning, tilting and zooming) by using the simple table based compensation for non-ideal camera parameters. We propose the threshold distance based homographic line generation algorithm. This takes account of uncertain parameters such as transformation error, height uncertainty of objects and synchronization issue between cameras. Thus, the proposed algorithm associates multiple objects on demand in the surveillance system where the camera movement dynamically changes. We verify the proposed method with actual image frames. Finally, we discuss the strategy to improve the association performance by using the temporal and spatial redundancy.

Estimation of damping induced by taut mooring lines

  • Xiong, Lingzhi;Lu, Wenyue;Li, Xin;Guo, Xiaoxian
    • International Journal of Naval Architecture and Ocean Engineering
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    • 제12권1호
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    • pp.810-818
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    • 2020
  • A moored floating structure may exhibit resonant motion responses to low-frequency excitations. Similar to the resonant responses of many vibration systems, the motion amplitude of a moored floating structure is significantly affected by the damping of the entire system. In such cases, the damping contributed by the mooring lines sometimes accounts for as much as 80% of the total damping. While the damping induced by catenary mooring lines is well-investigated, few studies have been conducted on the damping induced by taut mooring lines, especially one partly embedded in soil. The present study develops a simple but accurate model for estimating the damping contributed by mooring lines. A typical type of taut mooring line was used as the reference and the hydrodynamic drag force and soil resistance were taken into consideration. The proposed model was validated by comparing its predictions with those of a previously developed model and experimental measurements obtained by a physical model. Case studies and sensitivity studies were also conducted using the validated model. The damping induced by the soil resistance was found to be considerably smaller than the hydrodynamic damping. The superposition of the wave frequency motion on the low-frequency motion was also observed to significantly amplify the damping induced by the mooring lines.

A Mathematical Model for Converting Conveyor Assembly Line to Cellular Manufacturing

  • Kaku, Ikou;Gong, Jun;Tang, Jiafu;Yin, Yong
    • Industrial Engineering and Management Systems
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    • 제7권2호
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    • pp.160-170
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    • 2008
  • This paper proposes a mathematical model for converting conveyor assembly line to cellular manufacturing in complex production environments. Complex production environments refer to the situations with multi-products, variant demand, different batch sizes and the worker abilities varying with work stations and products respectively. The model proposed in this paper aims to determine (1) how many cells should be formatted; (2) how many workers should be assigned in each cell; (3) and how many workers should be rested in shortened conveyor line when a conveyor assembly line should be converted, in order to optimize system performances which are defined as the total throughput time and total labor power. We refer the model to a new production system. Such model can be used as an evaluation tool in the cases of (i) when a company wants to change its production system (usually a belt conveyor line) to a new one (including cell manufacturing); (ii) when a company wants to evaluate the performance of its converted system. Simulation experiments based on the data collected from the previous documents are used to estimate the marginal impact that each factor change has had on the estimated performance improvement resulting from the conversion.

공연장의 전기설비중 지락보호설비에 대한 현장실태분석 연구 (A Study on the Analysis of Field Condition for Ground Fault Protection Installation among Electrical Installations in the Entertainment Area)

  • 배석명;김한상;길형준;이건호
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2002년도 하계학술대회 논문집 C
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    • pp.1721-1723
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    • 2002
  • This paper deals with the analysis of field condition for earth leakage current alarming system in the stage lighting, stage sound stage machinery installation. These analyses of field condition were carried out in accordance with investigating an installation of earth leakage current alarming system with respect to a main line of power source, dimmer, sound equipment, machinery mobile unit equipment and so on. As a result of analyses. The earth leakage current alarming system has been installed only a part of the main line of power source and the probability of places which were installed was less than 50(%). Therefore, it is desirable that the earth leakage current alarming system is installed at places which are suitable, for example, dimmer, each kind machinery etc. in order to prevent electrical hazard.

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The behavior of adhesive joints affected by the geometry and stacking sequence of composite materials

  • Ait Kaci Djafar;Zagane Mohammed El Sallah;Moulgada Abdelmadjid;Sahli Abderahmane
    • Structural Engineering and Mechanics
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    • 제88권6호
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    • pp.609-623
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    • 2023
  • The objective of this study is to investigate the distribution of von Mises stress, peeling stress, and shear stress in the adhesive layer used to bond two composite panels, considering various parameters using a three-dimensional finite element method. The stiffness of the materials and the effect of the stacking order on the amount of load transferred to the adhesive layer were examined to determine which type of laminate generates less stress at the bond line. The study analyzed six different stacking sequences, all with a common first layer in contact with the adhesive and a 0° orientation. Additionally, the impact of using hybrid composites on reducing bond line stress was investigated.

회전기 고정자 권선에서의 운전 중 절연열화 진단기법 (On-Line Insulation Diagnosis Techniques for Stator Winding of Rotating Machines)

  • 윤대희;황돈하;신병철;김용주;이광식
    • 한국조명전기설비학회:학술대회논문집
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    • 한국조명전기설비학회 2000년도 학술대회논문집
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    • pp.89-93
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    • 2000
  • Recently many research activities on the diagnosis of stator winding insulation of large rotating machines have been reported. Capacitive couplers are widely used as sensors for on-line partial discharge (PD) measurement of high voltage rotating machines. This paper presents laboratory test to compare Stator Slot Coupler (SSC), Resistance Temperature Detector (RTD), 80 [pF] coupler and 500 [pF] coupler for on-line PD measurement of rotating machines. And a novel on-line monitoring system for an insulation diagnosis is proposed.

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Associations of Single Nucleotide Polymorphisms in BMPR-IB Gene with Egg Production in a Synthetic Broiler Line

  • Zhang, N.B.;Tang, H.;Kang, L.;Ma, Y.H.;Cao, D.G.;Lu, Y.;Hou, M.;Jiang, Y.L.
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권5호
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    • pp.628-632
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    • 2008
  • Egg production traits are economically important both for egg-laying and broiler lines of chicken. In sheep, the Q249R mutation in BMPR-IB is associated with ovulation rate. The present study cloned a partial chicken BMPR-IB fragment which contained the corresponding ovine Q249R mutation, including partial exon 6 and exon 7 and full-length intron 6. Five nucleotide changes were identified by alignment of the fragment amplified from Jining Bairi and Zang chickens. Among these nucleotide substitutions, the C/T transition at the base position of 35 and the A/G transition at the base position of 287 were found to be highly polymorphic, and named as SNPs C35T and A287G, respectively. For the SNP C35T, 331 hens of a synthetic broiler line were genotyped by a PCR-SSCP approach and allele C was found to be dominant. For the SNP A287G, 604 birds from the synthetic broiler line, a commercial egg-laying line, as well as three Chinese indigenous chicken breeds were genotyped by a PCR-RFLP technique. The associations of these two SNPs with egg production traits in the broiler line were analyzed. The results indicated that both the C35T and the A287G SNPs were not associated with egg production at 33wks and from 33wks to 42 wks (p>0.1), whereas the SNP A287G was associated with egg production from 47 to 56 wks (p<0.05). The dominance genetic effects on this latter trait and on egg production from 33 to 42 wks were significant (p<0.05).