• Title/Summary/Keyword: oilseed crops

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Biosynthesis of Polyunsaturated Fatty Acids: Metabolic Engineering in Plants (고도불포화지방산 생합성: 식물에서의 대사공학적 응용)

  • Kim, Sun-Hee;Kim, So-Yun;Kim, Jong-Bum;Roh, Kyung-Hee;Kim, Young-Mi;Park, Jong-Sug
    • Journal of Applied Biological Chemistry
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    • v.52 no.3
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    • pp.93-102
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    • 2009
  • Polyunsaturated fatty acids (PUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have significantly beneficial effects on health in relation to cardiovascular, immune, and inflammatory conditions and they are involved in determining the biophysical properties of membranes as well as being precursors for signaling molecules. PUFA biosynthesis is catalyzed by sequential desaturation and fatty acyl elongation reactions. This aerobic biosynthetic pathway was thought to be taxonomically conserved, but an alternative anaerobic pathway for the biosynthesis of PUFA is now known to contain analogous polyketide synthases (PKS). Certain fish oil can be a rich source of PUFA although processed marine oil is generally undesirable as food ingredients because of the associated objectionable flavors that are difficult and cost-prohibitive to remove. Oil-seed plants contain only the 18-carbon polyunsaturated fatty acid alpha-linolenic acid, which is not converted in the human body to EPA and DHA. It is now possible to engineer common oilseeds which can produce EPA and DHA and this has been the focus of a number of academic and industrial research groups. Recent advances and future prospects in the production of EPA and DHA in oilseed crops are discussed here.

Evaluation on the Effects of Deicing Salts on Crop using Seedling Emergence Assay of Oilseed Rape (Brassica napus) (유채의 출아 검정을 통한 제설제의 작물 영향 평가)

  • Lim, Soo-Hyun;Yu, Hyejin;Lee, Chan-Young;Gong, Yu-Seok;Lee, Byung-Duk;Kim, Do-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.1
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    • pp.72-79
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    • 2021
  • The increasing use of deicing salts has caused various environmental problems, including crop damage along the motorway where deicing salts are sprayed during winter. Deicing salts used on roads have been reported to negatively affect crops, but little information is known about their impact on crops. A seedling emergence assay was conducted to evaluate the effects of deicing salts on crops using oilseed rape (Brassica napus) as a model plant. We tested five chloride deicing salts consisting of NaCl, CaCl2, or MgCl2 and 1 non-chloride deicing salt (SM-3) at a range of concentrations (25, 50, 100, 200, and 400 mM), and untreated control. Regardless of deicing salts, they significantly delayed and reduced seedling emergence of oilseed rape with increasing salt concentration. Non-linear regression analysis of seedling emergence with a range of salt concentrations by fitting to the log-logistic model revealed that the chloride deicing salts reduced seedling emergence more than the non-chloride deicing salt SM-3. The GR50 value, the concentration causing 50% seedling emergence, of SM-3 was 47.1 mM, while those of the chloride deicing salts ranged from 30.7 mM (PC-10) to 37.5 mM (ES-1), showing approximately 10 mM difference between non-chloride and chloride deicing salts. Our findings suggest that seedling emergence assay is a useful tool to estimate the potential damage caused by deicing salts on crops.

SNP discovery and applications in Brassica napus

  • Hayward, Alice;Mason, Annaliese S.;Dalton-Morgan, Jessica;Zander, Manuel;Edwards, David;Batley, Jacqueline
    • Journal of Plant Biotechnology
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    • v.39 no.1
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    • pp.49-61
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    • 2012
  • This review summarises the biology, discovery and applications of single nucleotide polymorphisms in complex polyploid crop genomes, with a focus on the important oilseed crop $Brassica$ $napus$. $Brassica$ $napus$ is an allotetraploid species, and along with soybean and oil palm is one of the top three most important oilseed crops globally. Current efforts are well underway to $de$ $novo$ assemble the $B.$ $napus$ genome, following the release of the related $B.$ $rapa$ 'A' genome last year. The next generation of genome sequencing, SNP discovery and analysis pipelines, and the associated challenges for this work in $B.$ $napus$, will be addressed. The biological applications of SNP technology for both evolutionary and molecular geneticists as well as plant breeders and industry are far-reaching, and will be invaluable to our understanding and advancement of the $Brassica$ crop species.

Current status on plant molecular farming via chloroplast transformation (엽록체 형질전환 유래 분자 농업의 연구 동향)

  • Min, Sung-Ran;Jeong, Won-Joong;Kim, Suk-Weon;Lee, Jeong-Hee;Chung, Hwa-Jee;Liu, Jang-R.
    • Journal of Plant Biotechnology
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    • v.37 no.3
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    • pp.275-282
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    • 2010
  • Chloroplast transformation in higher plants offers many attractive advantages over nuclear transformation, including a high-level accumulation of foreign proteins, multi-gene expression in single transformation event via transgene stacking in operons and no position effect due to site-specific integration of transgenes by homologous recombination. Most importantly, chloroplast transgenic plants are eco-friendly because their transgenes are maternally inheritance in most crop plants. However, chloroplast transformation system has limited success in crops alike nuclear transformation. In the past two decades, great progress has been made to overcome the limitations of chloroplast transformation, thus expending chloroplast bioreactor to several important crops including soybean, carrot, lettuce, and oilseed. Therefore, it has become possible that chloroplast transformation of crops can be used not only for the improvement of agronomic traits, but also for the production of vaccines and high valuable therapeutic proteins in pharmaceutical industry.

Effect of Soil Salinity and Flooding on Plant Growth and Yield of Rape-Castor Bean Cropping System in the Newly Reclaimed Tidal Land of Western Seaside of Korea (서해안 신간척지에서 유채-피마자 작부체계시 토양염농도 및 침수가 생육 및 수량에 미치는 영향)

  • Sohn, Yong-Man;Jeon, Geon-Yeong;Song, Jae-Do;Lee, Jae-Hwang;Park, Moo-Eon
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.355-363
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    • 2009
  • Double cropping system of oilseed crops introduced rape and castor bean was studied in the newly reclaimed tidal land of Korea. Surface soil EC to reach at 50% of growth reduction to the tallest height of crops was estimated $4dS\;m^{-1}$ for castor bean and $6dS\;m^{-1}$ for rape by logarithmic function. The castor bean cultivated during rainy summer much more suffered serious growth and yield reduction by flooding damage in the Hwaong and Yeonsangang reclaimed lands having low soil conductivity and finer textured soil than in the Iweon reclaimed land having higher soil conductivity and coarse sandy textured soil. Rape cultivated during dry winter-spring much more suffered serious growth and yield reduction by high soil salinity come from re-salting process. 50% yield reduction was estimated at $2.0\;2.5dS\;m^{-1}$ of surface soil EC by logarithmic function. Consequentially, it was concluded that oilseed production for energy by double cropping system of rape and castor bean might be possible under good controling soil salinity below $4dS\;m^{-1}$ for castor and $3dS\;m^{-1}$ for rape in the newly reclaimed land of Korea.

Development of Selectable Marker of High Oleate Trait in Peanut (Arachis hypogaea L.) (땅콩에서 고 올레인산 형질관련 분자마커의 선발)

  • Yang, Kiwoung;Pae, Suk-Bok;Park, Chang-Hwan;Lee, Myoung Hee;Jung, Chan-Sik;Son, Jeong-Hee;Park, Keum-Yong
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.507-514
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    • 2010
  • Peanut(Arachis hypogaea L.) is one of the major oilseed crops. The peanut oil consists of palmitic, oleic and linoleic acids, which are present at levels of 10%, 36-67% and 15-43%, respectively. High oleate mutant of peanut F435 contains 80% oleate and as little as 2% linoleate in seed oil. Previous study indicated that delta 12 fatty acid desaturase is a major enzyme controlling the oleate content in seeds of oilseed crops. F435 sequence alignment of their coding regions disclosed that an extra A(adenine) was inserted at the position +2,823 bp of delta 12 fatty acid desaturase gene. This study was to develop molecular marker (SNP marker) co-segregating with the high oleate trait. Chopyeong ${\times}$ F435 $F_2$ 41 population were investigated using molecular marker and fatty acid assay (NIR and gas chromatography). Finally, this marker segregates Chopyeong type 26 lines, heterotype 9 lines and F435 type 6 lines. These results in our study suggested that SNP marker conform fatty acid assay.

Plant Toxins and Detoxification Methods to Improve Feed Quality of Tropical Seeds - Review -

  • Makkar, H.P.S.;Becker, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.3
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    • pp.467-480
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    • 1999
  • Many antinutritional and toxic factors abound in tropical seeds, which are also generally rich in nutrients and therefore more prone to attack from herbivores. Antinutritional and toxic factors are considered to defend seeds against environmental vagaries and thus help to protect them. These factors though good for the plant, cause deleterious effects or are even toxic to animals and man. The conventional seeds cultivated for oil or non-oil purposes, and general aspects of antinutritional factors are not presented here as these have already been discussed widely by many workers. Deficits in conventional protein and energy sources in the tropics have stimulated a quest for alternative feeds both for animals and humans. This article attempts to highlight two new oilseed crops, Jatropha curcas and Moringa oleifera, and in addition deals with some under-utilized seeds with potential as animal feed. Most of these seed plants are adapted to various marginal growing conditions in the tropics and can help to mitigate the prevailing deficit in protein and energy sources. Antinutritional and toxic factors in seed or seed meal, various approaches to detoxify seed meal, and future research and development priorities for their exploitation as animal feeds are presented.

Metabolic engineering for production of industrial oils in transgenic plants (식물 대사공학에 의한 산업용 지방산 생산연구 현황)

  • Lee, Kyeong-Ryeol
    • Journal of Plant Biotechnology
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    • v.36 no.2
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    • pp.97-105
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    • 2009
  • Seed storage lipids of plants, essential for seed germination as energy supplier, have been used for humankind and animal as nutrition sources. Fatty acids of vegetable oils have the characters appropriate for industry based on their chain length, the position and the number of double bonds. So they are used as raw materials for lubricants, cosmetics, soaps, paints and plastics or as energy source such as bio-diesel. However, there is a limit that applies vegetable oils from typical oil crops for industrial uses, mainly because of the mixture of five common fatty acids. Therefore, identification of unusual fatty acids for industrial uses from diverse plant resources and metabolic engineering to produce unusual fatty acids have been carried out in Arabidopsis as a model for the study of oilseed biology. Here, we discuss the unusual fatty acids for industrial uses, the genes synthesizing them in lipid metabolism, and the current limits in production of transgenic plants accumulating unusual fatty acid in their seeds. In addition, we describe our work on metabolic engineering of Brassica napus for the production of the unusual fatty acid ricinoleic acid in the seed, because of its industrial uses.

Recent advances in tissue culture and genetic transformation system of switchgrass as biomass crop (바이오에너지 개발용 스위치그라스의 조직배양 및 형질전환 최근 연구동향)

  • Lee, Sang Il;Lim, Sung-Soo;Roh, Hee Sun;Kim, Jong Bo
    • Journal of Plant Biotechnology
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    • v.40 no.4
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    • pp.185-191
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    • 2013
  • Over the past decades, carbon dioxide concentration of the atmosphere of the world has increased significantly, and thereby the greenhouse effect has become a social issue. To solve this problem, new renewable energy sources including solar, hydrogen, geothermal, wind and bio-energy are suggested as alternatives. Among these new energy sources, bio-energy crops are widely introduced and under rapid progress. For example, corn and oilseed rape plants are used for the production of bio-ethanol and bio-diesel, respectively. However, grain prices has increased severely because of the use of corn for bio-ethanol production. Therefore, non-edible switchgrass draws attention as an alternative source for bio-ethanol production in USA. This review describes the shortage of fossil energy and an importance of switchgrass as a bio-energy crop. Also, some characteristics of its major cultivars are introduced including growth habit, total output of biomass yields. Furthermore, biotechnological approaches have been conducted to improve the productivity of switchgrass using tissue culture and genetic transformation.

Analysis of Production and Trade of Agro-products in Myanmar (미얀마 주요 농산물의 생산 및 수출입 동향 분석)

  • Yim, Duk-Soon
    • Journal of the Korean Society of International Agriculture
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    • v.23 no.5
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    • pp.485-490
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    • 2011
  • In Southeast Asia, Myanmar is the largest country of the continent with land area 676,577 km2 that is three times higher than Korea. Nonetheless, a lot of diversity in agricultural enterprises is available in Myanmar as different climatic zones are available due to its unique location from north to south and east to west. Agriculture is considered the most important industry in Myanmar that largely relies on rice production. During 2009-10, rice and black gram are also among the major export items of Myanmar. Wheat, maize and sorghum are among the other major crops of the country and cultivation area of wheat and corn has been steadily increasing. Sesame is an important oilseed crop of Myanmar that is cultivated on the largest area. Presently, Myanmar has trade relations with Thailand, India and China.