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Development of Selectable Marker of High Oleate Trait in Peanut (Arachis hypogaea L.)  

Yang, Kiwoung (Department of Functional Crop, NICS, RDA)
Pae, Suk-Bok (Department of Functional Crop, NICS, RDA)
Park, Chang-Hwan (Department of Functional Crop, NICS, RDA)
Lee, Myoung Hee (Department of Functional Crop, NICS, RDA)
Jung, Chan-Sik (Department of Functional Crop, NICS, RDA)
Son, Jeong-Hee (Department of Functional Crop, NICS, RDA)
Park, Keum-Yong (Department of Functional Crop, NICS, RDA)
Publication Information
Korean Journal of Breeding Science / v.42, no.5, 2010 , pp. 507-514 More about this Journal
Abstract
Peanut(Arachis hypogaea L.) is one of the major oilseed crops. The peanut oil consists of palmitic, oleic and linoleic acids, which are present at levels of 10%, 36-67% and 15-43%, respectively. High oleate mutant of peanut F435 contains 80% oleate and as little as 2% linoleate in seed oil. Previous study indicated that delta 12 fatty acid desaturase is a major enzyme controlling the oleate content in seeds of oilseed crops. F435 sequence alignment of their coding regions disclosed that an extra A(adenine) was inserted at the position +2,823 bp of delta 12 fatty acid desaturase gene. This study was to develop molecular marker (SNP marker) co-segregating with the high oleate trait. Chopyeong ${\times}$ F435 $F_2$ 41 population were investigated using molecular marker and fatty acid assay (NIR and gas chromatography). Finally, this marker segregates Chopyeong type 26 lines, heterotype 9 lines and F435 type 6 lines. These results in our study suggested that SNP marker conform fatty acid assay.
Keywords
High oleate peanut; Delta 12 fatty acid desaturase; SNP (single nucleotide polymorphism) marker; MAS (marker assisted selection);
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