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SNP discovery and applications in Brassica napus

  • Hayward, Alice (Centre for Integrative Legume Research and School of Agriculture and Food Sciences, University of Queensland) ;
  • Mason, Annaliese S. (Centre for Integrative Legume Research and School of Agriculture and Food Sciences, University of Queensland) ;
  • Dalton-Morgan, Jessica (Centre for Integrative Legume Research and School of Agriculture and Food Sciences, University of Queensland) ;
  • Zander, Manuel (Centre for Integrative Legume Research and School of Agriculture and Food Sciences, University of Queensland) ;
  • Edwards, David (Australian Centre for Plant Functional Genomics, School of Agriculture and Food Sciences, University of Queensland) ;
  • Batley, Jacqueline (Centre for Integrative Legume Research and School of Agriculture and Food Sciences, University of Queensland)
  • Received : 2012.03.05
  • Accepted : 2012.03.15
  • Published : 2012.03.31

Abstract

This review summarises the biology, discovery and applications of single nucleotide polymorphisms in complex polyploid crop genomes, with a focus on the important oilseed crop $Brassica$ $napus$. $Brassica$ $napus$ is an allotetraploid species, and along with soybean and oil palm is one of the top three most important oilseed crops globally. Current efforts are well underway to $de$ $novo$ assemble the $B.$ $napus$ genome, following the release of the related $B.$ $rapa$ 'A' genome last year. The next generation of genome sequencing, SNP discovery and analysis pipelines, and the associated challenges for this work in $B.$ $napus$, will be addressed. The biological applications of SNP technology for both evolutionary and molecular geneticists as well as plant breeders and industry are far-reaching, and will be invaluable to our understanding and advancement of the $Brassica$ crop species.

Keywords

References

  1. Appleby N, Edwards D, Batley J (2009) New technologies for ultra-high throughput genotyping in plants, in: D Somers, et al. (Eds.), Plant genomics, Methods in Molecular Biology: Humana Press, USA. pp 19-40
  2. Atwell S, Huang YS, Vilhjálmsson BJ, Willems G, Horton M, Li Y, Meng D, Platt A, Tarone AM, Hu TT, Jiang R, Muliyati NW, Zhang X, Amer MA, Baxter I, Brachi B, Chory J, Dean C, Debieu M, Meaux Jd, Ecker JR, Faure N, Kniskern JM, Jones JDG, Michael T, Nemri A, Roux F, David E. Salt, Tang C, Todesco M, Traw MB, Weigel D, Marjoram P, Borevitz JO, Bergelson J, Nordborg M (2010) Genome-wide association study of 107 phenotypes in Arabidopsis thaliana inbred lines. Nature 465:627-631 https://doi.org/10.1038/nature08800
  3. Barker G, Batley J, O'Sullivan H, Edwards KJ, Edwards D (2003) Redundancy based detection of sequence polymorphisms in expressed sequence tag data using AutoSNP. Bioinformatics 19:421-422 https://doi.org/10.1093/bioinformatics/btf881
  4. Batley J, Edwards D (2007) SNP applications in plants, in: NC Oraguzie, et al. (Eds.), Association mapping in plants, Springer, New York. pp 95-102
  5. Batley J, Edwards D (2009) Genome sequence data: management, storage and visualization. Biotechniques 46:333-336 https://doi.org/10.2144/000113134
  6. Batley J, Jewell E, Edwards D (2007a) Automated discovery of Single Nucleotide Polymorphism (SNP) and Simple Sequence Repeat (SSR) molecular genetic markers, in: D Edwards (Ed.), Plant bioinformatics, Methods in Molecular Biology, Humana Press, USA. pp 473-494
  7. Batley J, Barker G, O'Sullivan H, Edwards KJ, Edwards D (2003a) Mining for Single Nucleotide Polymorphisms and insertions/ deletions in maize Expressed Sequence Tag data. Plant Physiol 132:84-91. https://doi.org/10.1104/pp.102.019422
  8. Batley J, Mogg R, Edwards D, O'Sullivan H, Edwards KJ (2003b) A high-throughput SNuPE assay for genotyping SNPs in the flanking regions of Zea mays sequence tagged simple sequence repeats. Mol Breeding 11:111-120 https://doi.org/10.1023/A:1022446021230
  9. Batley J, Hopkins CJ, Cogan NOI, Hand M, Jewell E, Kaur J, Kaur S, Li X, Ling AE, Love C, Mountford H, Todorovic M, Vardy M, Walkiewicz M, Spangenberg GC, Edwards D (2007b) Identification and characterisation of Simple Sequence Repeat (SSR) markers from Brassica napus expressed sequences. Mol Ecol Notes 7:886-889 https://doi.org/10.1111/j.1471-8286.2007.01738.x
  10. Berkman PJ, Lai K, Lorenc MT, Edwards D (2012a) Next generation sequencing applications for wheat crop improvement. Am J Bot 99:365-371 https://doi.org/10.3732/ajb.1100309
  11. Berkman PJ, Skarshewski A, Manoli S, Lorenc MT, Stiller J, Smits L, Lai K, Campbell E, Kubaláková M, Simkova H, Batley J, Dolezel J, Hernandez P, Edwards D (2012b) Sequencing wheat chromosome arm 7BS delimits the 7BS/4AL translocation and reveals homoeologous gene conservation. Theor Appl Genet 124:423-432. https://doi.org/10.1007/s00122-011-1717-2
  12. Berkman PJ, Skarshewski A, Lorenc MT, Lai K, Duran C, Ling EYS, Stiller J, Smits L, Imelfort M, Manoli S, McKenzie M, Kubaláková M, Simkova H, Batley J, Fleury D, Dolezel J, Edwards D (2011) Sequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS. Plant Biotech J 9:768-775 https://doi.org/10.1111/j.1467-7652.2010.00587.x
  13. Bhattramakki D, Rafalski A (2001) Discovery and application of single nucleotide polymorphism markers in plants, in: RJ Henry (Ed.), Plant genotyping: the DNA fingerprinting of plants, CABI Publishing, Wallingford, Oxon. pp 179-193
  14. Bhattramakki D, Dolan M, Hanafey M, Wineland R, Vaske D, Register JC, Tingey SV, Rafalski A (2002) Insertion-deletion polymorphisms in 3' regions of maize genes occur frequently and can be used as highly informative genetic markers. Plant Mol Biol 48:539-547 https://doi.org/10.1023/A:1014841612043
  15. Cardon LR, Bell JI (2001) Association study designs for complex diseases. Nat Rev Genet 2:91-99
  16. CCC (2010) Canola Council of Canada - Official definition of canola, http://www.canolacouncil.org/ind_definition.aspx.
  17. Chagne D, Batley J, Edwards D, Forster JW (2007) Single Nucleotide Polymorphisms genotyping in plants, in: NC Oraguzie, et al. (Eds.), Association mapping in plants, Springer, New York. pp 77-94
  18. Chen S, Nelson MN, Chèvre AM, Jenczewski E, Li Z, Mason AS, Meng J, Plummer JA, Pradhan A, Siddique KHM, Snowdon RJ, Yan G, Zhou W, Cowling WA (2011) Trigenomic bridges for Brassica improvement. Crit Rev Plant Sci 30:524-547 https://doi.org/10.1080/07352689.2011.615700
  19. Chevre AM, Barret P, Eber F, Dupuy P, Brun H, Tanguy X, Renard M (1997) Selection of stable Brassica napus-B. juncea recombinant lines resistant to blackleg (Leptosphaeria maculans). 1. Identification of molecular markers, chromosomal and genomic origin of the introgression. Theor Appl Genet 95:1104-1111 https://doi.org/10.1007/s001220050669
  20. Ching A, Caldwell KS, Jung M, Dolan M, Smith OSH, Tingey S, Morgante M, Rafalski AJ (2002) SNP frequency, haplotype structure and linkage disequilibrium in elite maize inbred lines. BMC Genet 3:19 https://doi.org/10.1186/1471-2156-3-19
  21. Choi SR, Teakle GR, Plaha P, Kim JH, Allender CJ, Beynon E, Piao ZY, Soengas P, Han TH, King GJ, Barker GC, Hand P, Lydiate DJ, Batley J, Edwards D, Koo DH, Bang JW, Park BS, Lim YP (2007) The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theor Appl Genet 115:777-792 https://doi.org/10.1007/s00122-007-0608-z
  22. Cowling WA (2007) Genetic diversity in Australian canola and implications for crop breeding for changing future environments. Field Crops Res 104:103-111 https://doi.org/10.1016/j.fcr.2006.12.014
  23. Cowling WA, Balazs E (2010) Prospects and challenges for genome-wide association and genomic selection in oilseed Brassica species. Genome 53:1024-1028 https://doi.org/10.1139/G10-087
  24. Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Heled J, Kearse M, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A (2011) Geneious v5.5, Available from http://www.geneious.com
  25. Duran C, Edwards D, Batley J (2009a) Genetic maps and the use of synteny, in: DJ Somers, et al. (Eds.), Plant genomics, Methods in Molecular Biology, Humana Press, USA. pp 41-56
  26. Duran C, Edwards D, Batley J (2009b) Molecular marker discovery and genetic map visualisation, in: D Edwards, et al. (Eds.), Applied Bioinformatics, Springer, USA. pp 165-189
  27. Duran C, Appleby N, Edwards D, Batley J (2009c) Molecular genetic markers: discovery, applications, data storage and visualisation. Curr Bioinform 4:16-27. https://doi.org/10.2174/157489309787158198
  28. Duran C, Boskovic Z, Batley J, Edwards D (2011) Role of bioinformatics as a tool for vegetable Brassica species, in: J Sadowski (Ed.), Vegetable Brassicas, Science Publishers Inc., New Hampshire. pp 406-418
  29. Duran C, Boskovic Z, Imelfort M, Batley J, Hamilton NA, Edwards D (2010a) CMap3D: A 3D visualisation tool for comparative genetic maps. Bioinformatics 26:273-274 https://doi.org/10.1093/bioinformatics/btp646
  30. Duran C, Appleby N, Clark T, Wood D, Imelfort M, Batley J, Edwards D (2009d) AutoSNPdb: an annotated Single Nucleotide Polymorphism database for crop plants. Nucleic Acids Res 37:951-953 https://doi.org/10.1093/nar/gkn650
  31. Duran C, Eales D, Marshall D, Imelfort M, Stiller J, Berkman P, Clark T, McKenzie M, Appleby N, Batley J, Basford K, Edwards D (2010b) Future tools for association mapping in crop plants. Genome 53:1017-1023 https://doi.org/10.1139/G10-057
  32. Durstewitz G, Polley A, Plieske J, Luerssen H, Graner EM, Wieseke R, Ganal MW (2010) SNP discovery by amplicon sequencing and multiplex SNP genotyping in the allopolyploid species Brassica napus. Genome 53:948-956 https://doi.org/10.1139/G10-079
  33. Edwards D (2007) Bioinformatics and plant genomics for staple crops improvement, in: MS Kang and PM Priyadarshan (Eds.), Breeding major food staples, Blackwell. pp 93-106
  34. Edwards D, Batley J (2004) Plant bioinformatics: from genome to phenome. Trends Biotechnol 22:232-237 https://doi.org/10.1016/j.tibtech.2004.03.002
  35. Edwards D, Batley J (2008) Bioinformatics: fundamentals and applications in plant genetics, mapping and breeding, in: C Kole and AG Abbott (Eds.), Principles and practices of plant genomics, Science Publishers Inc., USA. pp 269-302
  36. Edwards D, Batley J (2010) Plant genome sequencing: applications for crop improvement. Plant Biotech J 8:2-9 https://doi.org/10.1111/j.1467-7652.2009.00459.x
  37. Edwards D, Wang X (2012) Genome sequencing initiatives, in: D Edwards, et al. (Eds.), Genetics, genomics and breeding of oilseed Brassicas, Science Publishers Inc., New Hampshire, USA. pp 152-157
  38. Edwards D, Hansen D, Stajich J (2009) DNA sequence databases, in: D Edwards, et al. (Eds.), Applied bioinformatics, Springer, USA. pp 1-11
  39. Edwards D, Burton WA, Hopkins CJ, Batley J (2007a) Indian mustard, in: C Kole (Ed.), Genome mapping and molecular breeding in plants, Springer, New York. pp 179-210
  40. Edwards D, Forster JW, Chagné D, Batley J (2007b) What are SNPs?, in: NC Oraguzie, et al. (Eds.), Association mapping in plants, Springer, New York. pp 41-52
  41. Edwards D, Batley J, Cogan NOI, Forster JW, Chagne D (2007c) Single Nucleotide Polymorphism discovery, in: NC Oraguzie, et al. (Eds.), Association mapping in plants, Springer, New York. pp 53-76
  42. Edwards D, Batley J, Cogan NOI, Forster JW, Chagne D (2007d) Single Nucleotide Polymorphism discovery, in: NC Oraguzie, et al. (Eds.), Association mapping in plants, Springer, New York
  43. Erwin T, Jewell E, Love C, Lim G, Li X, Chapman R, Batley J, Stajich J, Mongin E, Stupka E, Ross B, Spangenberg GC, Edwards D (2007) BASC: an integrated bioinformatics system for Brassica research. Nucleic Acids Res 35:D870-D873 https://doi.org/10.1093/nar/gkl998
  44. Fan JB, Chee MS, Gunderson KL (2006) Highly parallel genomic assays. Nat Rev Genet 7:632-644 https://doi.org/10.1038/nrg1901
  45. Flint-Garcia SA, Thornsberry JM, Buckler ES (2003) Structure of linkage disequilibrium in plants. Ann Rev Plant Biol 54: 357-374 https://doi.org/10.1146/annurev.arplant.54.031902.134907
  46. Fourmann M, Barret P, Froger N, Baron C, Charlot F, Delourme R, Brunel D (2002) From Arabidopsis thaliana to Brassica napus: development of amplified consensus genetic markers (ACGM) for construction of a gene map. Theor Appl Genet 105:1196-1206 https://doi.org/10.1007/s00122-002-1040-z
  47. Friedt W, Snowdon R (2009) Oilseed rape, in: J Vollmann and I Rajcan (Eds.), Oil crops, Springer Science & Business Media.
  48. Hansen M, Hallden C, Nilsson N-O, Sall T (1997) Marker-assisted selection of restored male-fertile Brassica napus plants using a set of dominant RAPD markers. Mol Breeding 3:449-456 https://doi.org/10.1023/A:1009647417936
  49. Hayward A, McLanders J, Campbell E, Edwards D, Batley J (2012) Genomic advances will herald new insights into the Brassica:Leptosphaeria maculans pathosystem. Plant Biol 14:1-10
  50. Hernandez P, Martis M, Dorado G, Pfeifer M, Gálvez S, Schaaf S, Jouve N, Simková H, Valárik M, Dolezel J, Mayer KFX (2012) Next generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content. Plant J 69:377-386 https://doi.org/10.1111/j.1365-313X.2011.04808.x
  51. Hong CP, Piao ZY, Kang TW, Batley J, Yang TJ, Hur YK, Bhak J, Edwards D, Lim YP (2007) Genomic distribution of Simple Sequence Repeats in Brassica rapa. Mol Cells 23:349-356
  52. Hong CP, Plaha P, Koo DH, Yang TJ, Choi SR, Lee YK, Uhm T, Bang JW, Edwards D, Bancroft I, Park BS, Lee J, Lim YP (2006) A survey of the Brassica rapa genome through BAC-end sequence analysis, and comparative analysis with Arabidopsis thaliana. Mol Cells 22:300-307
  53. Hopkins C, Mogg R, Gororo N, Salisbury PA, Burton WA, Love C, Spangenberg GC, Edwards D, Batley J (2006) An assessment of genetic diversity within and between Brassica napus and Brassica juncea lines from international germplasm collections. Acta Hortic 706:115-119
  54. Hopkins CJ, Cogan NOI, Hand M, Jewell E, Kaur J, Li X, Lim GAC, Ling AE, Love C, Mountford H, Todorovic M, Vardy M, Spangenberg GC, Edwards D, Batley J (2007) Sixteen new simple sequence repeat markers from Brassica juncea expressed sequences and their cross-species amplification. Mol Ecol Notes 7:697-700 https://doi.org/10.1111/j.1471-8286.2007.01681.x
  55. Hou H, Zhao F, Zhou L, Zhu E, Teng H, Li X, Bao Q, Wu J, Sun Z (2010) MagicViewer: integrated solution for next-generation sequencing data visualization and genetic variation detection and annotation. Nucleic Acids Res 38:732-736 https://doi.org/10.1093/nar/gkq302
  56. Howell PM, Marshall DF, Lydiate DJ (1996) Towards developing intervarietal substitution lines in Brassica napus using marker-assisted selection. Genome 39:348-358 https://doi.org/10.1139/g96-045
  57. Imelfort M, Edwards D (2009) De novo sequencing of plant genomes using second-generation technologies. Brief Bioinform 10:609-618 https://doi.org/10.1093/bib/bbp039
  58. Imelfort M, Duran C, Batley J, Edwards D (2009a) Discovering genetic polymorphisms in next-generation sequencing data. Plant Biotech J 7:312-317 https://doi.org/10.1111/j.1467-7652.2009.00406.x
  59. Imelfort M, Batley J, Grimmond S, Edwards D (2009b) Genome sequencing approaches and successes, in: D Somers, et al. (Eds.), Plant Genomics, Methods in Molecular Biology, Humana Press, USA
  60. Kaur S, Cogan NOI, Ye G, Baillie RC, Hand ML, Ling AE, Mcgearey AK, Kaur J, Hopkins CJ, Todorovic M, Mountford H, Edwards D, Batley J, Burton W, Salisbury P, Gororo N, Marcroft S, Kearney G, Smith KF, Forster JW, Spangenberg GC (2009) Genetic map construction and QTL mapping of resistance to blackleg (Leptosphaeria maculans) disease in Australian canola (Brassica napus L.) cultivars. Theor Appl Genet120:71-83 https://doi.org/10.1007/s00122-009-1160-9
  61. Lai K, Berkman PJ, Lorenc MT, Duran C, Smits L, Manoli S, Stiller J, Edwards D (2012) WheatGenome.info: An integrated database and portal for wheat genome information. Plant Cell Physiol (In Press, accepted October 2011)
  62. Lee H, Lai K, Lorenc MT, Imelfort M, Duran C, Edwards D (2012) Bioinformatics tools and databases for analysis of next generation sequence data. Briefings in Functional Genomics 11:12-24 https://doi.org/10.1093/bfgp/elr037
  63. Lim GAC, Jewell EG, Li X, Erwin TA, Love C, Batley J, Spangenberg G, Edwards D (2007) A comparative map viewer integrating genetic maps for Brassica and Arabidopsis. BMC Plant Biol 7:40 https://doi.org/10.1186/1471-2229-7-40
  64. Lorenc M, Boskovic Z, Stiller J, Duran C, Edwards D (2012) Role of bioinformatics as a tool for oilseed Brassica species, in: D Edwards, et al. (Eds.), Genetics, genomics and breeding of oilseed Brassicas, Science Publishers Inc., New Hampshire. pp. 194-205
  65. Love C, Logan E, Erwin T, Spangenberg G, Edwards D (2006a) Analysis of the Brassica A and C genomes and comparison with the genome of Arabidopsis thaliana. Acta Hort 706: 99-104
  66. Love C, Robinson A, Lim G, Hopkins C, Batley J, Barker G, Spangenberg GC, Edwards D (2005) Brassica ASTRA: an integrated database for Brassica genomic research. Nucleic Acids Res 33:W493-W495 https://doi.org/10.1093/nar/gki462
  67. Love C, Logan E, Erwin T, Kaur J, Lim GAC, Hopkins C, Batley J, James N, May S, Spangenberg G, Edwards D (2006b) Integrating and interrogating diverse Brassica data within an EnsEMBL structured database. Acta Hort 706:77-82
  68. Love CG, Edwards D (2007) Accessing integrated Brassica genetic and genomic data using the BASC server, in: D Edwards (Ed.), Plant Bioinformatics, Methods in Molecular Biology, Humana Press, USA. pp 229-244
  69. Love CG, Batley J, Lim G, Robinson AJ, Savage D, Singh D, Spangenberg GC, Edwards D (2004) New computational tools for Brassica genome research. Comp Funct Genom 5:276-280 https://doi.org/10.1002/cfg.394
  70. Lysak MA, Koch MA (2011) Phylogeny, genome, and karyotype evolution of crucifers (Brassicaceae), in: R Schmidt and I Bancroft (Eds.), Genetics and Genomics of the Brassicaceae, Plant Genetics and Genomics: Crops and Models 9, Springer Science + Business Media, LLC
  71. Marshall DJ, Hayward A, Eales D, Imelfort M, Stiller J, Berkman PJ, Clark T, McKenzie M, Lai K, Duran C, Batley J, Edwards D (2010) Targeted identification of genomic regions using TAGdb. Plant Methods 6:19 https://doi.org/10.1186/1746-4811-6-19
  72. MBGP Multinational Brassica Genome Project - www.Brassica.info
  73. Milne I, Bayer M, Cardle L, Shaw P, Stephen G, Wright F, Marshall D (2010) Tablet - next generation sequence assembly visualization. Bioinformatics 26:401-402 https://doi.org/10.1093/bioinformatics/btp666
  74. Mogg R, Batley J, Hanley S, Edwards D, O'Sullivan H, Edwards KJ (2002) Characterisation of the flanking regions of Zea Mays microsatellites reveals a large number of useful sequence polymorphisms. Theor Appl Genet 105:532-543 https://doi.org/10.1007/s00122-002-0897-1
  75. Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia CR, Sasaki T (1997) Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol Breeding 3:87-103 https://doi.org/10.1023/A:1009651919792
  76. Morinaga T (1934) Interspecific hybridisation in Brassica VI. The cytology of F1 hybrids of B. juncea and B. nigra. Cytologia 6:62-67 https://doi.org/10.1508/cytologia.6.62
  77. Mun JH, Kwon SJ, Seol YJ, Kim JA, Jin M, Kim JS, Lim MH, Lee SI, Hong JK, Park TH, Lee SC, Kim BJ, Seo MS, Baek S, Lee MJ, Shin JY, Hahn JH, Hwang YJ, Lim KB, Park JY, Lee J, Yang TJ, Yu HJ, Choi SR, Ramchiary N, Lim YP, Fraser F, Drou N, Soumpourou E, Trick M, Bancroft I, Parkin IAP, Batley J, Edwards D, Park BS (2010) Sequence and structure of Brassica rapa chromosome A3. Genome Biol 11:R94 https://doi.org/10.1186/gb-2010-11-9-r94
  78. Neale DB, Savolainen O (2004) Association genetics of complex traits in conifers. Trends Plant Sci 9:325-330 https://doi.org/10.1016/j.tplants.2004.05.006
  79. Oraguzie NC (2007) An overview of association mapping, in: NC Oraguzie, et al. (Eds.), Association mapping in plants, Springer Science + Business Media, New York
  80. Orsouw NJv, Hogers RCJ, Janssen A, Yalcin F, Snoeijers S, Verstege E, Schneiders H, Poel Hvd, Oeveren Jv, Verstegen H, Eijk MJTv (2007) Complexity reduction of polymorphic sequences ($CRoPS^{TM}$): A Novel Approach for Large-Scale Polymorphism Discovery in Complex Genomes. PLoS ONE 2:e1172 https://doi.org/10.1371/journal.pone.0001172
  81. Picoult-Newberg L, Ideker TE, Pohl MG, Taylor SL, Donaldson MA, Nickerson DA, Boyce-Jacino M (1999) Mining SNPs from EST databases. Genome Res 9:167-174
  82. Pilet ML, Delourme R, Foisset N, Renard M (1998) Identification of loci contributing to quantitative field resistance to blackleg disease, causal agent Leptosphaeria maculans (Desm.) Ces. et de Not., in Winter rapeseed (Brassica napus L.). Theor Appl Genet 96:23-30 https://doi.org/10.1007/s001220050704
  83. Qiu D, Morgan C, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I (2006) A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theor Appl Genet 114:67-80 https://doi.org/10.1007/s00122-006-0411-2
  84. Rahman M, Sun Z, McVetty PBE, Li G (2008) High throughput genome-specific and gene-specific molecular markers for erucic acid genes in Brassica napus (L.) for marker-assisted selection in plant breeding. Theor Appl Genet117:895-904 https://doi.org/10.1007/s00122-008-0829-9
  85. Raymer PL (2002) Canola: an emerging oilseed crop, in: J Janick and A Whipkey (Eds.), Trends in new crops and new uses, ASHS Press, Alexandria
  86. Schranz ME, Song B-H, Windsor AJ, Mitchell-Olds T (2007) Comparative genomics in the Brassicaceae: a family-wide perspective. Curr Opin Plant Biol10:168-175 https://doi.org/10.1016/j.pbi.2007.01.014
  87. Smooker AM, Wells R, Morgan C, Beaudoin F, Cho K, Fraser F, Bancroft I (2011) The identification and mapping of candidate genes and QTL involved in the fatty acid desaturation pathway in Brassica napus. Theor Appl Genet 122:1075-1090 https://doi.org/10.1007/s00122-010-1512-5
  88. Snowdon RJ, Friedt W (2004) Molecular markers in Brassica oilseed breeding: current status and future possibilities. Plant Breeding 123:1-8 https://doi.org/10.1111/j.1439-0523.2003.00968.x
  89. Somers DJ, Rakow G, Prabhu VK, Friesen KRD (2001) Identification of a major gene and RAPD markers for yellow seed coat colour in Brassica napus. Genome 44:1077-1082 https://doi.org/10.1139/gen-44-6-1077
  90. Song K, Osborn TC (1992) Polyphyletic origins of Brassica napus: new evidence based on organelle and nuclear RFLP analyses. Genome 35:992-1001 https://doi.org/10.1139/g92-152
  91. Stokes D, Fraser F, Morgan C, O'Neill CM, Dreos R, Magusin A, Szalma S, Bancroft I (2010) An association transcriptomics approach to the prediction of hybrid performance. Mol Breeding 26:91-106 https://doi.org/10.1007/s11032-009-9379-3
  92. Syvanen A-C (2001) Accessing genetic variation: genotyping single nucleotide polymorphisms. Nat Rev Genet 2:930-942 https://doi.org/10.1038/35103535
  93. Taiyan Z, Lianli L, Guang Y, Al-Shehbaz IA (2001) Brassicaceae, in: Z Wu and PH Raven (Eds.), Flora of China, Science Press, Beijing
  94. Tanhuanpaa PK, Vilkki JP, Vilkki HJ (1995) Association of a RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.). Genome 38:414-416 https://doi.org/10.1139/g95-053
  95. The Brassica rapa Genome Sequencing Project Consortium, Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun J-H, Bancroft I, Cheng F, Huang S, Li X, Hua W, Wang J, Wang X, Freeling M, Pires JC, Paterson AH, Chalhoub B, Wang B, Hayward A, Sharpe AG, Park B-S, Weisshaar B, Liu B, Li B, Liu B, Tong C, Song C, Duran C, Peng C, Geng C, Koh C, Lin C, Edwards D, Mu D, Shen D, Soumpourou E, Li F, Fraser F, Conant G, Lassalle G, King GJ, Bonnema G, Tang H, Wang H, Belcram H, Zhou H, Hirakawa H, Abe H, Guo H, Wang H, Jin H, Parkin IAP, Batley J, Kim J-S, Just J, Li J, Xu J, Deng J, Kim JA, Li J, Yu J, Meng J, Wang J, Min J, Poulain J, Wang J, Hatakeyama K, Wu K, Wang L, Fang L, Trick M, Links MG, Zhao M, Jin M, Ramchiary N, Drou N, Berkman PJ, Cai Q, Huang Q, Li R, Tabata S, Cheng S, Zhang S, Zhang S, Huang S, Sato S, Sun S, Kwon S-J, Choi S-R, Lee T-H, Fan W, Zhao X, Tan X, Xu X, Wang Y, Qiu Y, Yin Y, et al. (2011) The genome of the mesopolyploid crop species Brassica rapa. Nat Genet 43:1035-1039 https://doi.org/10.1038/ng.919
  96. Trick M, Long Y, Meng J, Bancroft I (2009) Single nucleotide polymorphism (SNP) discovery in the polyploid Brassica napus using Solexa transcriptome sequencing. Plant Biotech J 7:334-346 https://doi.org/10.1111/j.1467-7652.2008.00396.x
  97. U N (1935) Genome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jap J Bot 7:389-452
  98. UNFAO, http://www.fao.org/index_en.htm, Food and Agriculture Organization of the United Nations
  99. Westermeier P, Wenzel G, Mohler V (2009) Development and evaluation of single-nucleotide polymorphism markers in allotetraploid rapeseed (Brassica napus L.). Theor Appl Genet 119:1301-1311 https://doi.org/10.1007/s00122-009-1135-x
  100. Zou J, Zhu J, Huang S, Tian E, Xiao Y, Fu D, Tu J-x, Fu T-d, Meng J (2010) Broadening the avenue of intersubgenomic heterosis in oilseed Brassica. Theor Appl Genet 120:283-290 https://doi.org/10.1007/s00122-009-1201-4

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