Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Development of Selectable Marker of High Oleate Trait in Peanut (Arachis hypogaea L.)

땅콩에서 고 올레인산 형질관련 분자마커의 선발

  • 양기웅 (국립식량과학원 기능성작물부) ;
  • 배석복 (국립식량과학원 기능성작물부) ;
  • 박장환 (국립식량과학원 기능성작물부) ;
  • 이명희 (국립식량과학원 기능성작물부) ;
  • 정찬식 (국립식량과학원 기능성작물부) ;
  • 손정희 (국립식량과학원 기능성작물부) ;
  • 박금룡 (국립식량과학원 기능성작물부)
  • Received : 2010.11.23
  • Published : 2010.12.31
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Abstract

Peanut(Arachis hypogaea L.) is one of the major oilseed crops. The peanut oil consists of palmitic, oleic and linoleic acids, which are present at levels of 10%, 36-67% and 15-43%, respectively. High oleate mutant of peanut F435 contains 80% oleate and as little as 2% linoleate in seed oil. Previous study indicated that delta 12 fatty acid desaturase is a major enzyme controlling the oleate content in seeds of oilseed crops. F435 sequence alignment of their coding regions disclosed that an extra A(adenine) was inserted at the position +2,823 bp of delta 12 fatty acid desaturase gene. This study was to develop molecular marker (SNP marker) co-segregating with the high oleate trait. Chopyeong ${\times}$ F435 $F_2$ 41 population were investigated using molecular marker and fatty acid assay (NIR and gas chromatography). Finally, this marker segregates Chopyeong type 26 lines, heterotype 9 lines and F435 type 6 lines. These results in our study suggested that SNP marker conform fatty acid assay.

땅콩의 delta 12 fatty acid desaturase 유전자의 염기서열에서 고 올레인산을 함유하는 F435를 선발할 수 있는 PCR 기초 분자표지마커를 제작하였다. 본 연구는 포인트 뮤테이션(point mutation)의 결과로 나타나는 고 올레인산을 SNP 마커를 이용하여 하나의 염기서열차이(아데닌 삽입)를 이용하여 판별할 수 있는 분자마커이다. 제작한 마커가 고 올레인산 관련 특이 마커인지 확인하기 위하여, 일반 땅콩 9 품종과 F435를 PCR 후 아가로스 겔 상에서 확인하여 고 올레인산 특이 분자마커임을 확인하였고, 조평 ${\times}$ F435의 $F_2$ 교배조합 41 계통을 이용하여 분리양상을 확인하였다. 그 결과 지방산 분석을 하지 않고도 고 올레인산 함유 계통을 손쉽게 선발할수 있었다. 특히, 헤테로 형질을 나타내는 계통도 선발 가능하였다. 분자마커의 결과가 지방산 수준에서 일치하는지 확인하기 위해 NIR 및 가스크로마토그래피 분석결과를 알아보았데 고 올레인산 관련 분자표지마커가 NIR 및 가스크로마토그래피 분석결과와 일치하였다. 고 올레인산 땅콩의 유전적 차이를 이용하여 분자표지마커를 개발 함으로서 고 올레인산 땅콩의 정확한 선발과 품종을 개발하는데 있어서 세대단축의 효과를 가져올 것이다.

Keywords

Acknowledgement

Supported by : 국립식량과학원

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