• 한국연초학회지
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• 제23권1호
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• pp.65-70
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• 2001

In order to compare the extraction patterns of main components from the raw material between the extraction methods, the aerial parts (dried stem, leaves, and flowers) of Agastache rugosa were extracted by SDE simultaneous steam distillation & extraction) and SFE (supercritical fluid extraction). Volatile components of essential oil and extract were identified by GC and GC-MSD. The contents of essential oil extracted by SDE were 0.49% in aerial part of Agastache rugosa on dry basis. Major components were methyl chavicol(27.2%), isomenthone(24.6%), hexadecanoic acid(13.0%). menthone (5.5%) among 32 kinds of components confirmed in essential oil. On the other hand, the contents of SFE extracts revealed 3.21% on dry basis, 6 times higher than those of SDE. Major components were isomenthone(15.3%), hexadecanoic acid(13.7%), methyl chavicol(12.6%), benzoic acid(3.8%) among 33 kinds of components identified in extract.

• 에너지공학
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• 제2권1호
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• pp.68-74
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• 1993

본 연구에서는 감압잔사유의 활용을 극대화하는 것을 목적으로 초임계 용매 추출법을 이용하여 탈아스팔트오일을 추출하였다. 초임계 용매로 n-pentane을 사용하였으며 온도와 압력 변화에 따른 추출수율과 추출된 오일 중 금속성분 및 황성분 함량의 변화가 조사되었다. 탈아스팔트오일의 추출수율은 사용한 용매의 초임계 영역에서 밀도에 크게 좌우됨이 조사되었고 추출유의 금속성분 함량은 감압잔사유에 비하여 크게 제거되었으나, 황성분 제거율은 낮았다.

• 한국식품영양학회지
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• 제12권4호
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• pp.358-363
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• 1999

This study was conducted to evaluate the extraction methods for the determination of antioxidants in soybean oil. Recovery rates of various antioxidants in soybean oil showed similar rates as 80.4~102.1% by solvent/solvent extraction method and 89.9~106.4% by sweep co-distillation method except 46.6~61.2% of PG at corresponding spiked concentractions. The maximun recovery rates of antioxidnts were obtained when extraction time and extraction temperature used in UNITREX were 20min and 21$0^{\circ}C$ respectively. In the recovery rates with the activation of florisil when 2% ofwater was added to florisil the highest recovery rates for TBHQ, BHA, BHT were obtained by sweep co-distillation met-hod. Therefore sweep co-distillation method showed less solvent simple operation and high recovery rate compared with solvent/solvent extraction method.

• Journal of Ginseng Research
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• 제41권3호
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• pp.428-433
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• 2017

Background: In this study, the fermentation of ginseng seeds was hypothesized to produce useful physiologically-active substances, similar to that observed for fermented ginseng root. Ginseng seed was fermented using Bacillus, Pediococcus, and Lactobacillus strains to extract ginseng seed oil, and the extraction yield, color, and quantity of phenolic compounds, fatty acids, and phytosterol were then analyzed. Methods: The ginseng seed was fermented inoculating 1% of each strain on sterilized ginseng seeds and incubating the seeds at $30^{\circ}C$ for 24 h. Oil was extracted from the fermented ginseng seeds using compression extraction, solvent extraction, and supercritical fluid extraction. Results and Conclusion: The color of the fermented ginseng seed oil did not differ greatly according to the fermentation or extraction method. The highest phenolic compound content recovered with the use of supercritical fluid extraction combined with fermentation using the Bacillus subtilis Korea Food Research Institute (KFRI) 1127 strain. The fatty acid composition did not differ greatly according to fermentation strain and extraction method. The phytosterol content of ginseng seed oil fermented with Bacillus subtilis KFRI 1127 and extracted using the supercritical fluid method was highest at 983.58 mg/100 g. Therefore, our results suggested that the ginseng seed oil fermented with Bacillus subtilis KFRI 1127 and extracted using the supercritical fluid method can yield a higher content of bioactive ingredients, such as phenolics, and phytosterols, without impacting the color or fatty acid composition of the product.

• 한국태양에너지학회 논문집
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• 제31권6호
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• pp.66-71
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• 2011

Various single solvents were tested to find the effective solvent for the extraction of algae oil from wet-form Chlorella minutissima. In the case of single solvents, their extractabilities for algae oil were increased with their polarity because the water in wet algae cell is to form a solvent shell around the lipids. Based on these results, the wet-form algae samples were treated with a polar alcohol solvent and then a nonpolar solvent was added in algae residue. In the algae oil extraction by ethanol/n-hexane, total lipid contents were 40-50% and composition of triglyceride in extracted oil was 46.50%. Considering solvent toxicity of conventional solvent mixture such as chloroform and methanol for algae oil extraction, the ethanol/n-hexane system was identified as the effective one for the oil extraction from wet-form Chlorella minutissima.

• Fisheries and Aquatic Sciences
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• 제25권2호
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• pp.76-89
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• 2022

The Lophius litulon liver was used as raw material for the extraction of fish oil via various extraction methods. The extraction rate by water extraction, potassium hydroxide (KOH) hydrolysis and protease hydrolysis were compared and the results revealed the protease hydrolysis extraction had a higher extraction rate with good protein-lipid separation as observed by optical microscope. Furthermore, subsequent experiments determined neutrase to be the best hydrolytic enzyme in terms of extraction rate and cost. The extraction conditions of neutrase hydrolysis were optimized by single-factor experiment and response surface analysis, and the optimal extraction rate was 58.40 ± 0.25% with the following conditions: enzyme concentration 2,000 IU/g, extraction time 1.0 h, liquid-solid ratio 1.95:1, extraction temperature 40.5℃ and pH 6.5. The fatty acids composition in fish oil from optimized extraction condition was composed of 19.75% saturated fatty acids and 80.25% unsaturated fatty acids. The content of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were 8.06% and 1.19%, respectively, with the ratio (6.77:1) surpassed to the recommendation in current researches (5:1). The results in this study suggest protease treatment is an efficient method for high-quality fish oil extraction from Lophius litulon liver with a satisfactory ratio of DHA and EPA.

• Food Science and Biotechnology
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• 제17권3호
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• pp.470-474
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• 2008

The whole berry, pulp, and seed of sea buckthorn fruit were extracted with supercritical $CO_2$ to produce edible oils. The effects of extraction pressure, temperature, and $CO_2$ flow rate on the oil yield and extraction rate were investigated, and the fatty acid composition, tocopherol, and carotenoid contents of the oils were compared. The results showed that the extraction rate was affected by pressure, temperature, and $CO_2$ flow rate and, in general, the yield increased with a rise in any of the 3 variables. Fatty acids in the whole berry and pulp oil were dominated by monounsaturated fatty acids (>64%), followed by saturated fatty acids (about 30%). In contrast, fatty acids in the seed oil consisted mainly of polyunsaturated (>60%) and monounsaturated fatty acids (>24%). The seed oil had a slightly higher content of tocopherols, but a much lower content of carotenoids, compared with the whole berry or pulp oil.

• 한국식품영양과학회지
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• 제43권3호
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• pp.439-445
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• 2014

본 연구에서는 추출방법에 따른 인삼씨유(ginseng seed oil)의 이화학적 품질특성과 저장 중 산화안정성을 살펴보았다. 인삼씨유를 용매추출, 초임계유체추출, 압착추출 방법으로 추출한 결과 초임계추출 한 인삼씨유의 수율이 가장 높았다. 인삼씨유의 L값은 8.70~11.38의 범위였고, a값은 초임계유체추출 오일 처리구에서, b값은 압착추출 오일에서 유의적으로 크게 나타났다. 지방산 조성은 불포화지방산이 대부분을 차지했으며, oleic acid(C18:1)의 함량이 80.13~81.16%로 가장 많았고 linoleic acid(C18:2)는 14.98~15.69%를 차지했다. 인삼씨유의 포화지방산으로는 유일하게 palmitic acid(16:0)만 검출되었다. 총 폴리페놀 함량은 압착추출 오일이 $56.32{\pm}1.47$ mg GAE/100g oil로 가장 높게 나타났다. 인삼씨유에서는 ${\gamma}$-토코페롤만 검출되었으며, 압착추출 오일의 ${\gamma}$-토코페롤 함량이 $56.32{\pm}1.47$ mg GAE/100g oil로 가장 높았다. 인삼씨유의 토코페롤 산화 유도시간은 압착추출 오일이 16.58시간으로 가장 길게 나타났다. 인삼씨유의 산가와 과산화물가는 저장기간에 따라 모두 증가하는 경향을 나타냈다. 결과적으로 압착추출 오일의 산화안정성이 가장 우수하게 나타났으며, 이는 항산화 활성을 지닌 총 페놀함량과 ${\gamma}$-토코페롤 함량이 초임계유체추출 오일과 용매추출 오일에서보다 압착추출 오일에서 가장 높게 나타났기 때문이라 판단된다.

• KSBB Journal
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• 제28권2호
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• pp.115-122
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• 2013

Volatile flavor compounds of Chamaecyparis obtuse essential oil were extracted by simultaneous steam distillation extraction (SDE) and supercritical fluid extraction (SFE) and analyzed by GC-MS. A total of 48 and 50 components were identified in essential oil by SDE and SFE, respectively. Monoterpenes, oxygenated monoterpenes, sesquiterpenes, oxygenated sesquiterpenes, and diterpenes in essential oil by SDE were 37.24, 10.9, 9.61, 0.22, and 0.22%, respectively. In the case of SFE, they were 19.1, 23.3, 22.66, 1.31, and 10.57%, respectively. Antioxidant activities were increased with the increase of essential oil up to $80{\mu}L/mL$, irrespective of extraction method. Especially, when the essential oil concentration extracted by SDE was increased from 20 to $80{\mu}L/mL$, the antioxidant activity was increased from 10.5 to 55.1%. However, over $80{\mu}L/mL$ of essential oil, an equilibrium state was maintained. In the case of essential oil extracted by SFE, it was decreased compared to that of SDE. For the improvement of atopic dermatitis, various cosmetics such as an ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE were tested. About over 90% was useful for the improvement of atopic dermatitis after 4 weeks of clinical trial targeting 40 female adults. These results demonstrate that ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE could be used in functional cosmetics.

• KSBB Journal
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• 제20권5호
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• pp.383-386
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• 2005

압착이나 유기용매 추출방식으로 생산된 포도씨유에는 resveratrol이 함유되어있지 않다 초임계 이산화탄소로 추출하는 경우 포도씨에 존재하는 resveratrol의 약 $1.6\%$가 추출 함유됨을 확인하였다. 초임계 추출 효율은 포도씨 건조 정도에 따라서 달라졌는데, 시료에 수분이 전혀 있지 않은 경우는 resvertrol을 추출되지 않았다. 수분함량이 증가할수록 추출 효율이 증가하는데, 수분이 resvertrol을 추출에 중요한 역할을 하였다. 초임계유체 추출 시간에 따른 추출된 포도씨 유의 resvertol 함량은 초반보다 후반으로 갈수록 증가하는 경향을 나타내었다. 초반에 오일 이 많이 추출되고 이후 수분이 빠져나오는 추출성향을 볼 때, resvertrol추출에는 수분이 초임계유체의 성질에 큰 영향을 미쳐 추출 효율을 변화시키는 것으로 생각된다.