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http://dx.doi.org/10.47853/FAS.2022.e8

Optimization of fish oil extraction from Lophius litulon liver and fatty acid composition analysis  

Hu, Zhiheng (College of Food Science and Technology, Hainan Tropical Ocean University)
Chin, Yaoxian (College of Food Science and Technology, Hainan Tropical Ocean University)
Liu, Jialin (College of Food Science and Technology, Hainan Tropical Ocean University)
Zhou, Jiaying (College of Food Science and Technology, Hainan Tropical Ocean University)
Li, Gaoshang (College of Food Science and Technology, Hainan Tropical Ocean University)
Hu, Lingping (College of Food Science and Technology, Hainan Tropical Ocean University)
Hu, Yaqin (College of Food Science and Technology, Hainan Tropical Ocean University)
Publication Information
Fisheries and Aquatic Sciences / v.25, no.2, 2022 , pp. 76-89 More about this Journal
Abstract
The Lophius litulon liver was used as raw material for the extraction of fish oil via various extraction methods. The extraction rate by water extraction, potassium hydroxide (KOH) hydrolysis and protease hydrolysis were compared and the results revealed the protease hydrolysis extraction had a higher extraction rate with good protein-lipid separation as observed by optical microscope. Furthermore, subsequent experiments determined neutrase to be the best hydrolytic enzyme in terms of extraction rate and cost. The extraction conditions of neutrase hydrolysis were optimized by single-factor experiment and response surface analysis, and the optimal extraction rate was 58.40 ± 0.25% with the following conditions: enzyme concentration 2,000 IU/g, extraction time 1.0 h, liquid-solid ratio 1.95:1, extraction temperature 40.5℃ and pH 6.5. The fatty acids composition in fish oil from optimized extraction condition was composed of 19.75% saturated fatty acids and 80.25% unsaturated fatty acids. The content of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were 8.06% and 1.19%, respectively, with the ratio (6.77:1) surpassed to the recommendation in current researches (5:1). The results in this study suggest protease treatment is an efficient method for high-quality fish oil extraction from Lophius litulon liver with a satisfactory ratio of DHA and EPA.
Keywords
Fish oil; Lophius litulon liver; Protease; Response surface methodology;
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1 Vazquez JA, Menduina A, Nogueira M, Duran AI, Sanz N, Valcarcel J, et al. Optimal production of protein hydrolysates from monkfish by-products: chemical features and associated biological activities. Molecules. 2020;25:4068.   DOI
2 Wang YH, Kuo CH, Lee CL, Kuo WC, Tsai ML, Sun PP, et al. Enzyme-assisted aqueous extraction of cobia liver oil and protein hydrolysates with antioxidant activity. Catalysts. 2020;10:1323.   DOI
3 Wenwei C, Guangrong H, Zhenbao J, Yao H. Optimization of aqueous enzymatic extraction of oil from shrimp processing by-products using response surface methodology. Food Sci Technol. 2019;39:231-6.   DOI
4 Forsyth S, Gautier S, Salem N Jr. Global estimates of dietary intake of docosahexaenoic acid and arachidonic acid in developing and developed countries. Ann Nutr Metab. 2016;68:258-67.   DOI
5 Lunn J, Theobald HE. The health effects of dietary unsaturated fatty acids. Nutr Bull. 2006;31:178-224.   DOI
6 Loftsson T, Ilievska B, Asgrimsdottir GM, Ormarsson OT, Stefansson E. Fatty acids from marine lipids: biological activity, formulation and stability. J Drug Deliv Sci Technol. 2016;34:71-5.   DOI
7 Stollewerk K, Jofre A, Comaposada J, Arnau J, Garriga M. Food safety and microbiological quality aspects of QDS process® and high pressure treatment of fermented fish sausages. Food Control. 2014;38:130-5.   DOI
8 Fang X, Fei X, Sun H, Jin Y. Aqueous enzymatic extraction and demulsification of camellia seed oil (Camellia oleifera Abel.) and the oil's physicochemical properties. Eur J Lipid Sci Technol. 2016;118:244-51.   DOI
9 Maqsood S, Benjakul S, Kamal-Eldin A. Extraction, processing, and stabilization of health-promoting fish oils. Recent Pat Food Nutr Agric. 2012;4:141-7.   DOI
10 Marsol-Vall A, Aitta E, Guo Z, Yang B. Green technologies for production of oils rich in n-3 polyunsaturated fatty acids from aquatic sources. Crit Rev Food Sci Nutr. 2021:1-21.
11 Adeoti IA, Hawboldt K. A review of lipid extraction from fish processing by-product for use as a biofuel. Biomass Bioenergy. 2014;63:330-40.   DOI
12 Qi-yuan L, Jun-qing Q, Xiao-ge W. Optimization of enzymatic fish oil extraction from mackerel viscera by response surface methodology. Int Food Res J. 2016;23:992-7.
13 Miyashita K, Uemura M, Hosokawa M. Effective prevention of oxidative deterioration of fish oil: focus on flavor deterioration. Annu Rev Food Sci Technol. 2018;9:209-26.   DOI
14 Mozaffarian D, Wu JHY. (n-3) fatty acids and cardiovascular health: are effects of EPA and DHA shared or complementary? J Nutr. 2012;142:614S-25S.   DOI
15 Menegazzo ML, Petenuci ME, Fonseca GG. Production and characterization of crude and refined oils obtained from the co-products of Nile tilapia and hybrid sorubim processing. Food Chem. 2014;157:100-4.   DOI
16 Rebah FB, Miled N. Fish processing wastes for microbial enzyme production: a review. 3 Biotech. 2013;3:255-65.   DOI
17 Haddar A, Fakhfakh-Zouari N, Hmidet N, Frikha F, Nasri M, Kamoun AS. Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone. J Biosci Bioeng. 2010;110:288-94.   DOI
18 Han Z, Xu S, Sun J, Yue X, Wu Z, Shao JH, et al. Effects of fatty acid saturation degree on salt-soluble pork protein conformation and interfacial adsorption characteristics at the oil/water interface. J. Food Hydrocoll. 2021;113:106472.   DOI
19 Jamshidi A, Cao H, Xiao J, Simal-Gandara J. Advantages of techniques to fortify food products with the benefits of fish oil. Food Res Int. 2020;137:109353.   DOI
20 Aitta E, Marsol-Vall A, Damerau A, Yang B. Enzyme-assisted extraction of fish oil from whole fish and by-products of baltic herring (Clupea harengus membras). Foods. 2021;10:1811.   DOI
21 Castejon N, Senorans FJ. Enzymatic modification to produce health-promoting lipids from fish oil, algae and other new omega-3 sources: a review. N Biotechnol. 2020;57:45-54.   DOI
22 Guo X, Zou X, Sun M. Optimization of extraction process by response surface methodology and preliminary characterization of polysaccharides from Phellinus igniarius. Carbohydr Polym. 2010;80:344-9.   DOI
23 Lin N, Mo X, Yang Y, Zhang H. Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes. Glycoconj J. 2017;34:241-53.   DOI
24 Kaur N, Chugh V, Gupta AK. Essential fatty acids as functional components of foods: a review. J Food Sci Technol. 2014;51:2289-303.   DOI
25 Koletzko B, Bergmann K, Brenna JT, Calder PC, Campoy C, Clandinin MT, et al. Should formula for infants provide arachidonic acid along with DHA? A position paper of the European Academy of Paediatrics and the Child Health Foundation. Am J Clin Nutr. 2020;111:10-16.
26 Laurenson CH, Priede IG. The diet and trophic ecology of anglerfish Lophius piscatorius at the Shetland islands, UK. J Mar Biol Assoc UK. 2005;85:419-24.   DOI
27 Luque de Castro MD, Priego-Capote F. Soxhlet extraction: past and present panacea. J Chromatogr A. 2010;1217:2383-9.   DOI
28 Rubio-Rodriguez N, Beltran S, Jaime I, de Diego SM, Sanz MT, Carballido JR. Production of omega-3 polyunsaturated fatty acid concentrates: a review. Innov Food Sci Emerg Technol. 2010;11:1-12.   DOI
29 Alfio VG, Manzo C, Micillo R. From fish waste to value: an overview of the sustainable recovery of omega-3 for food supplements. Molecules. 2021;26:1002.   DOI
30 Hathwar SC, Bijinu B, Rai AK, Narayan B. Simultaneous recovery of lipids and proteins by enzymatic hydrolysis of fish industry waste using different commercial proteases. Appl Biochem Biotechnol. 2011;164:115-24.   DOI
31 Sargent J, Bell G, McEvoy L, Tocher D, Estevez A. Recent developments in the essential fatty acid nutrition of fish. Aquaculture. 1999;177:191-9.   DOI
32 Swart J, Bordoloi A, Goosen NJ. Optimization of phosphate recovery from monkfish, Lophius vomerinus, processing by-products and characterization of the phosphate phases. J Sci Food Agric. 2019;99:2743-56.   DOI
33 Jensen CL, Maude M, Anderson RE, Heird WC. Effect of docosahexaenoic acid supplementation of lactating women on the fatty acid composition of breast milk lipids and maternal and infant plasma phospholipids. Am J Clin Nutr. 2000;71:S292-9.   DOI
34 Zhang J, Yi C, Han J, Ming T, Zhou J, Lu C, et al. Novel high-docosahexaenoic-acid tuna oil supplementation modulates gut microbiota and alleviates obesity in high-fat diet mice. Food Sci Nutr. 2020;8:6513-27.   DOI
35 Sahena F, Zaidul ISM, Jinap S, Saari N, Jahurul HA, Abbas KA, et al. PUFAs in fish: extraction, fractionation, importance in health. Compr Rev Food Sci Food Saf. 2009;8:59-74.   DOI
36 Shao JH, Deng YM, Zhou GH, Xu XL, Liu DY. A Raman spectroscopic study of meat protein/lipid interactions at protein/oil or protein/fat interfaces. Int J Food Sci Technol. 2015;50:982-9.   DOI
37 de Oliveira DASB, Minozzo MG, Licodiedoff S, Waszczynskyj N. Physicochemical and sensory characterization of refined and deodorized tuna (Thunnus albacares) by-product oil obtained by enzymatic hydrolysis. Food Chem. 2016;207:187-94.   DOI
38 Blum JM, Su Q, Ma Y, Valverde-Perez B, Domingo-Felez C, Jensen MM, et al. The pH dependency of N-converting enzymatic processes, pathways and microbes: effect on net N2O production. Environ Microbiol. 2018;20:1623-40.   DOI
39 Brault H, Miketinas D. Adequate intake of docosahexaenoic acid for adults: NHANES 2015-2016 (P18-032-19). Curr Dev Nutr. 2019;3:nzz039.
40 Dragalin I, Morarescu O, Sedcenco M, Rosca RM. GC-MS analysis of the fatty acids methyl esters in Japanese quail fat. Chem J Mold. 2015;10:54-7.   DOI
41 Gulzar S, Raju N, Nagarajarao RC, Benjakul S. Oil and pigments from shrimp processing by-products: extraction, composition, bioactivities and its application- a review. Trends Food Sci Technol. 2020;100:307-19.   DOI
42 Aguilera-Oviedo J, Yara-Varon E, Torres M, Canela-Garayoa R, Balcells M. Sustainable synthesis of omega-3 fatty acid ethyl esters from monkfish liver oil. Catalysts. 2021;11:100.   DOI
43 Let MB, Jacobsen C, Pham KA, Meyer AS. Protection against oxidation of fish-oil-enriched milk emulsions through addition of rapeseed oil or antioxidants. J Agric Food Chem. 2005;53:5429-37.   DOI
44 Luo D. Optimization of total polysaccharide extraction from Dioscorea nipponica Makino using response surface methodology and uniform design. Carbohydr Polym. 2012;90:284-8.   DOI
45 Ghasemi Fard S, Wang F, Sinclair AJ, Elliott G, Turchini GM. How does high DHA fish oil affect health? A systematic review of evidence. J Crit Rev Food Sci Nutr. 2019;59:1684-727.   DOI
46 Zhu Y, Yu J, Jiao C, Tong J, Zhang L. Optimization of quercetin extraction method in Dendrobium officinale by response surface methodology. Heliyon. 2019;5:e02374.   DOI
47 Xu J, Li Y, Regenstein J, Su X. In vitro and in vivo anti-oxidation and anti-fatigue effect of monkfish liver hydrolysate. Food Biosci. 2017;18:9-14.   DOI
48 Xie D, Gong M, Wei W, Jin J, Wang X, Wang X, et al. Antarctic krill (Euphausia superba) oil: a comprehensive review of chemical composition, extraction technologies, health benefits, and current applications. Compr Rev Food Sci Food Saf. 2019;18:514-34.   DOI
49 Yang ZH, Amar M, Sampson M, Courville AB, Sorokin AV, Gordon SM, et al. Comparison of omega-3 eicosapentaenoic acid versus docosahexaenoic acid-rich fish oil supplementation on plasma lipids and lipoproteins in normolipidemic adults. Nutrients. 2020;12:749.   DOI
50 Ying Y, Xiang Y, Liu J, Chen X, Hu L, Li Y, et al. Optimization of ultrasonic-assisted freezing of Penaeus chinensis by response surface methodology. Food Qual Saf. 2021;5:fyaa034.   DOI
51 Zhang J, Cui C, Chen H, Liu J. The completion of esterification of free fatty acids in Zanthoxylum bungeanum seed oil with ethanol. Int J Green Energy. 2014;11:822-32.   DOI
52 Zhu BW, Qin L, Zhou DY, Wu HT, Wu J, Yang JF, et al. Extraction of lipid from sea urchin (Strongylocentrotus nudus) gonad by enzyme-assisted aqueous and supercritical carbon dioxide methods. Eur Food Res Technol. 2010;230:737-43.   DOI
53 Zhang Y, Li S, Yin C, Jiang D, Yan F, Xu T, et al. Response surface optimisation of aqueous enzymatic oil extraction from bayberry (Myrica rubra) kernels. Food Chem. 2012;135:304-8.   DOI