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Open Pulled Straws, EM Grid, Nylon Loop법에 의한 돼지성숙난자의 유리화동결 비교

  • 안미현;김인덕;석호봉
    • Proceedings of the KSAR Conference
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    • 2003.06a
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    • pp.83-83
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    • 2003
  • 돼지 난자의 유리화 동결 처리 방법 중 난자를 담는 용기(loading vessel)의 재료로 최근에 알려진 것으로 open-pulled straws(OPS)[Vajta등, Mol Reprod Dev, 51:53-58, (1998)], electron microscope grids(EMG) [Martino등,Biol Reprod, 54:1059-1069, (1996)〕, nylon loop system(NLS) [Lane등, Fertil Steril,72: 1073-1078, (1999)] 등이 보고되고 있다. OPS는 1/4cc straws를 열을 가하여 길게 뽑아 내벽을 얇게 함으로써 filing된 난자나 수정란이 액체 질소와 접촉했을 때 유리화가 신속하게 되도록 하는 방법으로 돼지에서는 별로 보고된 것이 없다. EMG는 열전도가 예민한 전자현미경용 copper grid를 이용한 방법으로 최근 국내 기술진의 연구성적을 포함한 몇몇 학자들에 의하여 보고되었고 NLS는 0.5mm직경의 nylon loop를 이용하여 급속 동결한 성적이 보고되었으나, 돼지 난자에 응용 된 것은 없다. 따라서 이와 같은 동결 재료는 사람과 반추류, mouse외에 돼지 난자에 대하여는 전혀 시도되지 않았지만 유리화 동결기술에서 가장 중요한 실험으로 생각된다. 성공적인 유리화 동결을 위해서는 수정란이 냉각의 전도성이 빠르고, 작은 용액을 수정란과 같이 filling해야 하며 모든 동작이 신속 간편해야 하며 융해 방법도 초급속도의 융해가 요구되므로 이에 부합되어야 한다. 연구 목적은 돼지 난자를 유리화 동결/융해 시 동결 재료-straw/glass, copper grid, nylon 3가지에 대한 제작 방법, 난자 loading, 동결 처리, 보관 방법, 융해 방법 등을 난자의 회수, 수정 후 생존율을 비교 조사하여 가장 우수한 방법을 선택할 목적이었다. 수행 내용은 3가지의 재료의 sample을 제작하고 소독한 다음 준비된 돼지 COCs을 40시간동안 IVM한 후 난자를 5~l5개 정도로 선정 하여 준비된 VS 용액에 평형처리 하였다. 각 재료의 용기에 loading 한 후 동결/보관하였고, 융해는 역순으로 평형하여 maturation 배지에 3~4시간 배양한 다음 경검하고 IVF한 후 NCSU-23 배지에 담아 IVC 배양하면서 cell cleavage상태를 확인하였다.

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Study of Vitrification of Immatured Pig Oocytes: Compared with Open Pulled Straw(OPS), Electron Microscopic Grid(EMG) and Nylon Loop System(NLS) (미성숙 돼지 난자의 유리화 동결에 관한 연구: Open Pulled Straw(OPS), Electron Microscopic Grid(EMG) 및 Nylon Loop System(NLS)의 비교)

  • 김인덕;안미현;석호봉
    • Journal of Embryo Transfer
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    • v.19 no.1
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    • pp.27-34
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    • 2004
  • This study evaluated the efficiency and compared with different materials of loading vessels for vitrification-plastic/glass, copper grid and nylon. The loading method, vitrification, cryop-reservation and warming method of the oocytes were examined. The loading samples prepared in manual or company-made and sterilized, loaded the COCs selected on each samples and cultured for maturation during 40 hours, and then exposed sequentially to ethylene glycol solution. Thawing method was reversely treated and exposed for warmed oocytes. After oocytes were thawed, fertilized and cultured in vitro for 3-4 hours, rates of development and morphological appearance were examined. The results were as summarized: ㆍOPS from company-made or hand-made of the hematocrit micropipettes, NLS from fishing line and EMG from company-made for EM were used for loading oocytes, respectively. ㆍThe efficiency of freezing method and loading convenience were orderly higher in OPS, NLS and EMG. The optimal capacity per vessel was orderly lowered in NLS, EMG and OPS, respectively. ㆍAfter oocytes were warmed, the recovery rate, morphology and rate of development were orderly higher in OPS, NLS and EMG, respectively. ㆍIn conclusion, OPS has the advantages of achieving a little more survival and preserving results than other two loading methods.

Analysis on Physical Property of Para-aramid/Nylon Hybrid filaments according to the ATY nozzle Diameter (ATY Nozzle 직경변화에 따른 Aramid/Nylon Hybrid사의 ATY 물성)

  • Park, Mi-Ra;Choi, La-Hee;Ma, Hye-Young;Park, Sung-Woo;Kim, Seung-Jin
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.64-64
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    • 2012
  • 아라미드는 일반적인 유기섬유와는 다른 우수한 역학적 성질을 바탕으로 보호의류 중에서 방탄방호 및 방검보호 의류에 사용되는 고부가 소재이다. 현재까지 ATY기계에서 사의 구조와 물성에 큰 영향을 미치는 Nozzle의 구조에 대한 연구결과는 많이 발표되어왔다. 그러나 최근 들어 소방방화방 검용 보호의류에 많이 사용되는 아라미드사에 ATY 공정 중에서 Nozzle의 직경이 ATY사의 물성에 어떤 영향을 미치는가에 대한 연구는 발표된 바가 없다. 따라서 본 연구에서는 Para-aramid/Nylon hybrid사를 이용하여 ATY로 제조할 경우, 표면에 생기는 loop로 인하여 타 소재와 접착시, 접착제 담지 성능이 향상되어 접착력이 상승되는 반면 아라미드 Hybrid사의 역학물성은 ATY가 가공되기 전의 물성보다 저하되는 약점을 가지고 있다. 따라서 본 연구에서는 ATY 제조공정에서 Nozzle의 직경을 달리할 때 Aramid/Nylon Hybrid ATY사의 물성변화를 분석함으로서 방화복과 방검용 보호의류에 적합한 아라미드 ATY사를 개발하고자 한다. 본 연구에서는 ATY 제조공정 중 다른 공정조건은 동일하게 하고 Nozzle의 직경을 0.6, 0.75, 1, 1.2mm로 변경하여 4가지 시료를 준비하고 물성분석을 위하여 제조된 시료의 강신도, 초기탄성률을 각각 측정하여 인장특성을 확인하였으며, 건열수축률과 습열수축률을 측정하여 시료의 열 수축률을 측정 분석하였다. 표면의 루프 발현 정도를 보기위하여 형태 불안정성을 측정 평가하였으며 영상현미경시스템을 사용하여 표면특성을 측정 평가하여 다음과 같은 결론을 얻었다. Nozzle의 직경이 증가함에 따라 절단강도는 30% 감소하였고 초기탄성률은 3배 가까이 감소하였다. 그리고 절단신도는 2배정도 증가하는 경향을 나타내었다. 또한 Nozzle의 직경이 증가함에 따라 ATY hybrid사의 건 습열수축률이 증가하다가 직경이 1.2mm일 때 감소하는 경향을 나타내었고 직경 변화에 따라 4~6%의 열 수축률의 분포를 보였다. Para-aramid/Nylon hybrid사의 형태불안정성은 0.3~0.5%를 분포를 나타내었고 Nozzle의 직경이 0.6, 1mm일 때 상대적으로 낮은 ATY의 불안정성이 확인되었다. Nozzle의 직경이 감소할수록 loop의 엉킴이 적으며 flat하였으며 직경이 1.2mm일 때 가장 조밀하고 표면에 loop가 많이 형성된 것을 확인하였다.

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Compressional Properties of PTT BCF and Nylon BCF Carpets (PTT BCF카펫과 나일론 BCF카펫의 압축특성(壓縮特性)에 관(關)한 연구(硏究))

  • Yun, Myung-Hui;Kim, Jong-Jun;Jeon, Dong-Won
    • Journal of Fashion Business
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    • v.8 no.5
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    • pp.115-124
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    • 2004
  • PTT(polytrimethylene terephthalate) is a thermoplastic that can be melt-spun into fibers and has extensive applications in carpets, textiles and apparel, engineering thermoplastics, nonwovens, and films or sheets. This polymer combines the good properties of nylon and polyester. Compared with other synthetic fibers such as nylon and acrylic, the PTT fibers feel softer, dye easier with vibrant colors, stretch and recover better. Moreover, the PTT fibers for carpets resist most stainings, clean better, and dry faster. The PTT was first patented in 1941, but it was not until the 1990's, when Shell Chemicals developed the practical method of producing PDO, the raw material for PTT. Many studies have been done including the retention of carpet texture using an image analysis technique, or compressional resilience of the carpet for long term use. In this study, PTT and nylon BCF carpets were compared in terms of the compressional properties including the resilience, using one of the KES system for repetitive measurements. The compression resilience(RC) values of the PTT BCF carpets far exceed those of nylon 6 BCF carpets. The RC values of the PTT BCF carpet(cut) specimens are $42{\sim}45%$ for 5 successive compression deformations, while those of the nylon BCF carpet specimens(cut) are $26{\sim}28%$. There is also a similar trend in the RC values for the other type of carpet which is the loop type. This resilience is one of the important factors of carpet usage evaluation.

Treatment of Painful Hand Neuroma : To Make a Loop to Transpose the Nerve Ending to the Side of its Proximal Stump - Case Report - (수부 신경종의 치료 : 고리 모양의 단.측 신경봉합술의 이용 - 증례 보고 -)

  • Ko, Ra-Yong;Oh, Kap-Sung
    • Archives of Reconstructive Microsurgery
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    • v.8 no.1
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    • pp.92-96
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    • 1999
  • Neuroma is formed by abnormal, incomplete nerve regeneration after nerve injury. A painful neuroma in the hand can be psychologically and physically disabling. The goal of treating painful neuroma is to relieve pain and to restore nerve function. A numerous treatment modality was reported for alleviating the problem. These treatments include crushing the neuroma, ligating it, burying in soft tissue, bone, and muscle, injecting it with alcohol, phenol, and steroid, capping it with silicone cuff. But, none of these methods has been uniformly successful, although each has its advocates. No one technique reliably prevents formation of a painful neuroma. However, the principles of treatment is resection of neuroma and proximal stump of the nerve is transposed to appropriate adjacent tissue. Our current technique was resection of neuroma with partial normal neural tissue, and then the nerve ending was transposed and sutured to the side of the proximal stump with 10-0 nylon, so end-to-side neurorrhaphy was made. The nerve ending had to be placed and fixed into the proximal nerve epineurium like as a figure of a loop. We believe this technique is another useful method for the treatment of painful neuroma.

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The Modified Outside-In Technique for Meniscal Suture - Technical Note - (변형된 Outside-In 기법을 이용한 반월상 연골판 봉합술 - 수술 수기 -)

  • Lee Kee-Byoung;Kwon Duck-Joo;Lee Young-Gyun;Song Young-Joon
    • Journal of the Korean Arthroscopy Society
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    • v.7 no.1
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    • pp.96-99
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    • 2003
  • As arthroscopy is more advanced and the importance of meniscal function is more emphasized, there have been more advanced en meniscal repair technique. However conventional technique require the use of special instruments and extensive skin incision to protect the neurovasculatures and soft tissues. Also these have the potential problem of damage in articular surfaces by the knot. So, we develop a modified outside-in technique using spinal needles and nylon loops. Our method have many advantages not only in stability but simplicity, and there is no need of additional skin incision.

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Comparisons of Electrical Conductivity between Polyester/Polyurethane and Nylon/Polyurethane Woven or Knitted Fabrics with Silver Paste Patterns in Elongation-Strain test (폴리에스터/폴리우레탄 및 나일론/폴리우레탄에 은 문양을 입힌 편직물의 신장-변형 시 전기 전도도 비교)

  • Kim, Hyejin;Yun, Changsang;Kim, Jongjun
    • Journal of Fashion Business
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    • v.23 no.2
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    • pp.1-17
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    • 2019
  • The objective of this study was to investigate electrical conductivity of fabrics from polyester (PET) and Nylon (N) containing polyurethane (PU), with silver paste patterns screen-stenciled in three directions. The PET/PU and N/PU fabrics knitted or woven were uniaxially strain-recovered up to 22.5% in three times when each change in electrical resistance was simultaneously measured. This study established four variables that complexly affected electrical conductivity of these specimens; fabric structures, components, cover factors, and the percolation of silver particles. The woven or knitted fabric structures did not distinctively cause the changes in electrical resistance, however, the woven fabrics with the diagonal patterns showed their relatively high electrical resistance. The PET/PU fabrics with increasing the PET proportion generally presented the opposite propensity to its electrical conductivity. The changes in electric resistance of the PET/PU 85/15 2/1 twill and double plain fabrics instantaneously responded to the rate of elongation. The PET/PU group exhibited a reverse correlation between its cover factor and electrical resistivity. The highest electrical conductivity of the PET/PU 95/5 interlock fabric, with very few fluctuations, was attributed to the deep percolation of the silver particles that bridged the gaps between one loop and another. On the other hand, the occurrence of the silver cracks along with the elongated direction led to the immeasurably high change in electrical resistance as the strain increased.

Redoable Tie-Over Dressing Using Multiple Loop Silk Threads

  • Jo, Hyeon Jong;Kim, Jun Sik;Kim, Nam Gyun;Lee, Kyung Suk;Choi, Jae Hoon
    • Archives of Plastic Surgery
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    • v.40 no.3
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    • pp.259-262
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    • 2013
  • After skin grafting, to prevent hematoma or seroma collection at the graft site, a tie-over dressing has been commonly used. However, although the conventional tie-over dressing by suture is a useful method for securing a graft site, refixation is difficult when repeated tie-over dressing is needed. Therefore, we recommend a redoable tie-over dressing technique with multiple loops threads and connecting silk threads. After the raw surface of each of our cases was covered with a skin graft, multiple loop silk thread attached with nylon at the skin graft margin. We applied the ointment gauze and wet cotton/fluffy gauze over the skin graft, then fixed the dressing by connecting cross-counter multiple loop thread with connecting silk threads. When we opened the tie-over dressing by cutting the connecting silk threads, we repeated the tie-over dressing with the same method. The skin graft was taken successfully without hematoma or seroma collection or any other complications. In conclusion, we report a novel tie-over dressing enabling simple fixation of the dressing to maintain proper tension for wounds that require repetitive fixation. Further, with this reliable method, the skin grafts were well taken.