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Study of Vitrification of Immatured Pig Oocytes: Compared with Open Pulled Straw(OPS), Electron Microscopic Grid(EMG) and Nylon Loop System(NLS)  

김인덕 (단국대학교 생명자원과학대학 동물자원과학과)
안미현 (단국대학교 생명자원과학대학 동물자원과학)
석호봉 (단국대학교 생명자원과학대학 동물자원과학과)
Publication Information
Journal of Embryo Transfer / v.19, no.1, 2004 , pp. 27-34 More about this Journal
Abstract
This study evaluated the efficiency and compared with different materials of loading vessels for vitrification-plastic/glass, copper grid and nylon. The loading method, vitrification, cryop-reservation and warming method of the oocytes were examined. The loading samples prepared in manual or company-made and sterilized, loaded the COCs selected on each samples and cultured for maturation during 40 hours, and then exposed sequentially to ethylene glycol solution. Thawing method was reversely treated and exposed for warmed oocytes. After oocytes were thawed, fertilized and cultured in vitro for 3-4 hours, rates of development and morphological appearance were examined. The results were as summarized: ㆍOPS from company-made or hand-made of the hematocrit micropipettes, NLS from fishing line and EMG from company-made for EM were used for loading oocytes, respectively. ㆍThe efficiency of freezing method and loading convenience were orderly higher in OPS, NLS and EMG. The optimal capacity per vessel was orderly lowered in NLS, EMG and OPS, respectively. ㆍAfter oocytes were warmed, the recovery rate, morphology and rate of development were orderly higher in OPS, NLS and EMG, respectively. ㆍIn conclusion, OPS has the advantages of achieving a little more survival and preserving results than other two loading methods.
Keywords
porcine oocytes; vitrification; open pulled straw; EM grid; nylon loop);
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