• Journal of Microbiology and Biotechnology
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• v.31 no.1
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• pp.104-114
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• 2021

Petroleum-contaminated soil is considered among the most important potential anthropogenic atmospheric methane sources. Additionally, various rhizoremediation factors can affect methane emissions by altering soil ecosystem carbon cycles. Nonetheless, greenhouse gas emissions from soil have not been given due importance as a potentially relevant parameter in rhizoremediation techniques. Therefore, in this study we sought to investigate the effects of different plant and soil amendments on both remediation efficiencies and methane emission characteristics in diesel-contaminated soil. An indoor pot experiment consisting of three plant treatments (control, maize, tall fescue) and two soil amendments (chemical nutrient, compost) was performed for 95 days. Total petroleum hydrocarbon (TPH) removal efficiency, dehydrogenase activity, and alkB (i.e., an alkane compound-degrading enzyme) gene abundance were the highest in the tall fescue and maize soil system amended with compost. Compost addition enhanced both the overall remediation efficiencies, as well as pmoA (i.e., a methane-oxidizing enzyme) gene abundance in soils. Moreover, the potential methane emission of diesel-contaminated soil was relatively low when maize was introduced to the soil system. After microbial community analysis, various TPH-degrading microorganisms (Nocardioides, Marinobacter, Immitisolibacter, Acinetobacter, Kocuria, Mycobacterium, Pseudomonas, Alcanivorax) and methane-oxidizing microorganisms (Methylocapsa, Methylosarcina) were observed in the rhizosphere soil. The effects of major rhizoremediation factors on soil remediation efficiency and greenhouse gas emissions discussed herein are expected to contribute to the development of sustainable biological remediation technologies in response to global climate change.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.9
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• pp.659-666
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• 2014

The purpose of this study was to suggest a standard design procedure of landfarming for clean-up of oil-contaminated soils. The standard design procedure consisted of four main phases; soil characterization, determination of contaminated soil volume, determination of nutrient and microbial doses, and estimation of the total remedial period. This study selected standard design parameter values or ranges among various forms used in environmental engineer communities. Those were determination procedures for the contaminated soil volume, the initial contamination concentration and nutrient doses. The suggested standard design procedure were applied for a landfarm design for remediation of a real oil-contaminated site. Soil texture of the site was classified as sandy clay loam and sandy loam. Total nitrogen and total phosphorus were estimated to be 57.01 mg/kg and 83.40 mg/kg, respectively. Also the viable bacterial numbers was assessed to be $1.78{\times}10^4CFU/g$ dry soil. The amount of TPH contaminated soil was estimated to be $4,092m^3$. With the application of remedial factors, it was estimated that the contaminated soil could be treated through 9 batches with a duration of 315 days for a landfarming unit of $15m{\times}40m{\times}1m$. The amount of liquid microorganisms and fertilizers were recommended to be 4,025L and 4,641kg, respectively.

• Journal of Environmental Science International
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• v.13 no.12
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• pp.1059-1065
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• 2004

Bio sparging experiments were conducted in a laboratory column to investigate the potential removal of diesel contaminated groundwater. The objectives in this study were (a) to determine the extent of diesel degradation in laboratory columns under supplement of nutrient; (b) to determine the effect of variation of air flow in the removal of diesel and (c) to evaluate the potential enhancement of diesel degradation as a function of temperature. Our results showed that the nutrient supplement and higher air flow greatly enhanced diesel degradation. However, the variation of water temperature examined slightly increased degradation rate of diesel fuel.

• Journal of Ecology and Environment
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• v.41 no.9
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• pp.235-245
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• 2017

Background: Loading of excess nutrient via bioremediation of polluted sediment to overlying water could trigger anoxia and eutrophication in coastal area. The aim of this research was to understand the changes of overlying water features such as dissolved oxygen (DO); pH; oxidation reduction potential (ORP); $chlorophyll-{\alpha}$ ($Chl-{\alpha}$); and nitrogen nutrients ammonia ($N-NH_4{^+}$), nitrate ($N-NO_3{^-}$), and nitrite ($N-NO_2^-$) when the sediment was not treated (control) and treated by calcium peroxide for 5 weeks. Methods: The water samples were analyzed for measuring physical and chemical properties along with the sediment analyzed by polymerase chain reaction (PCR) including denaturing gradient gel electrophoresis (DGGE) for identifying the phylogenetic affiliation of microbial communities. Results: Results showed that due to the addition of calcium peroxide in sediment, the overlying water exposed the rise of dissolve oxygen, pH, and ORP than control. Among the nitrogen nutrients, ammonia inhibition was higher in calcium peroxide treatment than control but in case of nitrate inhibition, it was reversed than control. $Chlorophyll-{\alpha}$ was declined in treatment column water by 30% where it was 20% in control column water. Actibacter and Salegentibacter group were detectable in the calcium-peroxide-treated sediment; in contrary, no detectable community ware found in control sediment. Both phylogenetic groups are closely related to marine microflora. Conclusions: This study emphasizes the importance of calcium peroxide as an oxygen release material. Interaction with peroxide proved to be enhancing the formation of microbial community that are beneficial for biodegradation and spontaneity of nutrient attenuation into overlying water.

• ALGAE
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• v.31 no.3
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• pp.189-204
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• 2016

Microalgal bioremediation of CO₂, nutrients, endocrine disruptors, hydrocarbons, pesticides, and cyanide compounds have evaluated comprehensively. Microalgal mitigation of nutrients originated from municipal wastewaters, surface waters, and livestock wastewaters has shown great applicability. Algal utilization on secondary and tertiary treatment processes might provide unique and elegant solution on the removing of substances originated from various sources. Microalgae have displayed 3 growth regimes (autotrophic, heterotrophic, and mixotrophic) through which different organic and inorganic substances are being utilized for growth and production of different metabolites. There are still some technology challenges requiring innovative solutions. Strain selection investigation should be directed towards identification of algal that are extremophiles. Understanding and manipulation of metabolic pathways of algae will possible unfold solution to utilization of algae for mitigation of dissolve organic nitrogen in wastewaters.

• Journal of Soil and Groundwater Environment
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• v.14 no.4
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• pp.15-22
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• 2009

The remediation efficiency for a large scale petroleum-contaminated site was evaluated by using the Engineered Land-farming system which was consists of the following parameters; moisture & nutrient injector data, blower system, HDPE sheet and sump system. To enhance the degradation ability in the early stage, main nutrients such as nitrogen (N) and phosphorus (P) were adjusted for the site condition. As a result of the periodic tilling process, the concentration of contaminated soil was decreased to 348 mg/kg, which was lower than 500 mg/kg (regal standards) while satisfying remediation Efficiency of 82% (the maximum concentration of 1,893 mg/kg). The appropriate temperature range for an active operation was investigated between $28.9{\sim}35.6^{\circ}C$. For the contaminated soils having different initial concentration, the TPH (Total Petroleum Hydrocarbons) concentration was decreased evenly along with the CFU (Colony Forming Unit), moisture content and contaminant concentration after 38days of gratifying the legal standards of under 500 mg/kg.

• Journal of Environmental Impact Assessment
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• v.32 no.2
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• pp.112-122
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• 2023

To restore the ecological function of contaminated soil and maximize the ecological services provided by the soil, besides the toxicity orrisk caused by pollutants, the functional aspects of the soil ecosystem should be considered. In this study, a method for evaluating the health of cleaned soil was presented, and the applicability of the proposed evaluation method was examined by applying it to soil treated with washing and landfarming. Productivity, habitat, water retention capacity, nutrient cycling, carbon retention capacity, and buffering capacity were used as soil health evaluation indicators. The results showed that the soil health was not completely recovered after remediation, and even in the case of the washed soil, the health was lower than before remediation. On the other hand, there was no significant change in soil quality due to oil pollution, but soil health deteriorated. Unlike the slightly improved soil quality after landfarming treatment, soil health was not completely restored. Therefore, the results of this study indicate that it is desirable to consider both soil quality and health when evaluating the remediation effect. The soil health evaluation method proposed in this study can be usefully utilized for the sustainable use of cleaned soil and to promote ecosystem services.

• ALGAE
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• v.29 no.3
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• pp.227-235
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• 2014

The red seaweed, Gracilaria tikvahiae McLachlan, was cultivated in open water farms in urbanized estuaries of Long Island Sound (26-30 psu of salinity) and New York City (20-25 psu), USA in 2011. Plants were harvested monthly from summer (August, $24^{\circ}C$) to fall (November, $13^{\circ}C$) and analyzed for total nitrogen, protein, and amino acid content. On a dry matter (DM) basis, nitrogen and protein significantly increased over the harvest period until October and then plateaued. Nitrogen increased from $22{\pm}1g\;kg^{-1}$ DM in August to $39{\pm}3g\;kg^{-1}$ DM in October (p < 0.001). Protein increased from $107{\pm}13g\;kg^{-1}$ DM in August to $196{\pm}5g\;kg^{-1}$ DM in November (p < 0.001). With two exceptions, amino acid concentrations expressed on a crude protein (CP) basis were similar over the harvest period. Essential amino acids accounted for $48{\pm}1%$ of all amino acids present with lysine and methionine averaging $56{\pm}2g\;kg^{-1}$ CP and $18{\pm}1g\;kg^{-1}$ CP, respectively. Histidine was underrepresented among essential amino acids and averaged $13{\pm}1g\;kg^{-1}$ CP. Taurine ranged from 2.1 to $3.2g\;kg^{-1}$ DM. With its moderate levels of lysine, methionine and taurine, ocean farmed G. tikvahiae has the potential of overcoming many nutrient deficiencies currently associated with terrestrial plant ingredients in alternative feeds for fish and shrimp.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.4
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• pp.411-419
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• 2003

Dredged material during Masan Bay cleaning in 1990-1994 was deposited in Gapo area. The site provides an ideal experimental condition to monitor environmental remediation and benthic ecosystem stabilization processes after the disturbance. Sea water samples were taken during one tidal cycle in one hour interval from Oct. 2001 to Apr. 2002 (4 times) to estimate the organic matter and nutrient fluxes in Gapo area. Hourly material fluxes were estimated from the water balance estimated from 3 dimensional topography of Gapo area and from material concentration. Net material fluxes were estimated from the difference between total influx and total outflux during one tidal cycle. Chemical oxygen demand showed net outflux in Nov. 2001, Dec. 2001 and Apr. 2002 (2.2∼3.9 g m$\^$-2/ h$\^$-1/) and showed net influx in Mar. 2002 (1.4 g m$\^$-2/ h$\^$-1/). Ammonium showed net outflux during the study (0.1∼118 mg m$\^$-2/ h$\^$-1/m-2h-I). According to this investigation, Gapo area was a source rather than a sink of organic matter. However, the variability of the material fluxes was high so that a long term study may be required.

• Journal of Soil and Groundwater Environment
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• v.22 no.5
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• pp.82-88
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• 2017

Contaminated sediment can be treated in order to reuse the treated sediment. Even though the chemical criteria are satisfied, the treated sediment could still impose toxic effects. Therefore, this study investigated the changes in the ecological toxic effects of the contaminated sediment from the J region in Singapore after treatment. The contaminated sediment was subject to sequential soil washing and thermal treatment, followed by pH neutralization. Toxic effects of the contaminated and treated sediments were determined by using Vibrio fischeri ($Microtox^{(R)}$), Triticum aestivum (wheat), and Eisenia foetida (earthworm). After treatment, the concentrations of total petroleum hydrocarbons and heavy metals were decreased by 98% and 59-93%, respectively, and satisfied the Industrial Maximum Values of the Dutch Standard, which were used as the remedial goal. The bioluminescence reduction of V. fischeri decreased significantly, and the earthworm survival increased from 0% to 90% after treatment. The germination rate increased from $0{\pm}0%$ to $75{\pm}13%$ after treatment, but the treated sediment may need additional treatment such as nutrient addition for better plant growth. Overall, this study showed that the treatment of the contaminated sediment satisfactorily removed mixed contaminants, and this led to reduction in toxic effects, suggesting improved potentials for reuse of the treated sediment.