• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.813-818
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• 2003

This study was carried out to survey operation system of self-making TMR and particle size of self-making and commercial TMR, then to compare TMR particle size recommended by Penn State Particle Size Separator. Daily mixing time in self-making TMR averaged 48.6${\pm}$28.0min., 2 augar type of mixer was most by 55.5% and daily 2 times of mixing in summer and other season was 44.4 and 22.2%, respectively. Percentage of residual feed in upper sieve(19mm diameter), middle sieve(8 to 18mm diameter) and lower pen was 21.5~25.6%, 16.0~25.8%, 52.8~61.4%, respectively, at self-making TMR mixer. Percentage of particle sizes of more than 19mm in self-making and commercial TMR was 24.9${\pm}$1.4 and 26.2${\pm}$1.7%, that of 8-10mm 22.8${\pm}$1.0 and 12.8${\pm}$1.2 and that of less than 8mm 52.3${\pm}$1.7 and 61.0${\pm}$1.5, respectively, which in particle length of 8~13mm in self-making and commercial TMR was lower by 82.6 and 100% compared to that recommended by Penn State particle separator, respectively. Especially there was not particle length of more than 19mm in commercial TMR at all. It is necessary to check the number of cows ruminating in a farm in order to estimate the particle size of TMR; it is recommended to change the TMR mixing time or the TMR formulae if the proportion of ruminating cows in a farm is less than 40 %.

• Journal of Animal Science and Technology
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• v.55 no.3
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• pp.195-202
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• 2013

The present study was undertaken to investigate the effects of increasing pig market weight on the incidence of the 'caky-fatty' belly, carcass grade and carcass quality traits. To this end, 500 (Yorkshire ${\times}$ Landrace) ${\times}$ Duroc market pigs consisting of equal numbers of barrows and gilts were slaughtered at body weights ranging from 85 to 150 kg and their carcasses were analyzed. Backfat thickness (BFT; mm) of the barrows and gilts regressed on live weight (kg) linearly as follows: BFT (y) = 0.1827x + 3.4825 ($r^2$ = 0.4671) and y = 0.2015x-0.6972 ($r^2$ = 0.4736), respectively. The caky-fatty belly, which, by definition of the present study, had = 55% fat by weight in the dorsal-side half of a 5 cm-wide belly strip between the $11^{th}$ and $12^{th}$ ribs, was found in 10 carcasses from barrows. However, no belly was condemned as 'caky-fatty' by the retailers. Total number of carcasses which were down-graded on account of overweight was as many as 101 (20.2%). The $1^+A$-grade carcasses exhibited a superior intramuscular fat score to all other grades, but except for this and the defective quality traits, no visible differences were found in carcass quality among different carcass grades. In conclusion, the current carcass grading system, which is sparingly reflective of the carcass quality and also under-evaluates the larger but otherwise normal carcasses, may as well be revised. Moreover, the incidence of the caky-fatty belly appears not to be a critical factor to be considered when increasing the pig market weight.

• Journal of Animal Science and Technology
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• v.55 no.3
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• pp.185-194
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• 2013

Microsatellite markers have been a useful genetic tool in determining diversity, relationships and individual discrimination studies of livestock. The level of genetic diversity, relationships among two Korean indigenous chicken brand populations (Woorimatdag: WR, Hanhyup3: HH) as well as two pure populations (White Leghorn: WL, Rhode Island Red: RIR) were analyzed, based on 26 MS markers. A total of 191 distinct alleles were observed across the four chicken populations, and 47 (24.6%) of these alleles were unique to only one population. The mean $H_{Exp}$ and PIC were estimated as 0.667 and 0.630. Nei's $D_A$ genetic distance and factorial correspondence analysis (FCA) showed that the four populations represented four distinct groups. However, the genetic distance between each Korean indigenous chicken brand (WR, HH) and the pure population (WL, RIR) were threefold that among the WR and HH. For the STRUCTURE analyses, the most appropriate number of clusters for modeling the data was determined to be three. The expected probabilities of identity among genotypes of random individuals (PI) were calculated as $1.17{\times}10^{-49}$ (All 26 markers) and $1.14{\times}10^{-15}$, $7.33{\times}10^{-20}$ (9, 12 with the highest PI value, respectively). The results indicated that the brand chicken breed traceability system employing the own highest PI value 9 to 12 markers, and might be applicable to individual identification of Korean indigenous chicken brand.

• Applied Microscopy
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• v.7 no.1
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• pp.21-43
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• 1977

This experiment was undertaken in order to localize the labeled dbcAMP (dibutyryl cyclic AMP) in oocytes whose development has been suppressed by cold dbcAMP for 6 or 19 hours in vitro. Mouse oocytes were obtained from the ovaries of 3-4 week old A strain female mice, by puncturing the Graafian follicles in the modified Krebs-Ringer bicarbonate salt solution under the dissecting microscope. Those oocytes which have intact germinal vesicle were cultured in the basic culture medium supplemented with 0.4% bovine serum albumin (BSA). Cultivation of the oocytes was carried out in a microtube developed by Cho (1974). The cultures were then incubated in a humidified 5% $CO_2$ incubator maintained at $37^{\circ}C$ for 6 or 19 hours (Donahue, 1968). DbcAMP was added to culture medium for a final concentration of 100ug/ml, and $^3H-dbc$ AMP (specific activity 13 Ci/mM) for a final concentration of $40{\mu}Ci/ml$ was also added to the medium. For electron microscopic autoradiography, those oocytes recovered from the culture were washed with phosphate buffer (pH 7.4), and immediately prefixed in a 2.5% glutaraldehyde overnight and postfixed for 2 hours at $4 ^{\circ}C$ in 1% osmium tetroxide in phosphate buffer with pH 7.4 (Palade, 1952). After fixation, the materials were dehydrated in graded alcohol series and embedded in Epon 812 mixture based on the standard procedures (Luft, 1961). The thin sections $600-700{\AA}$ thick were mounted on the grids of 200 meshes. The grids containing sections were coated with a nuclear emulsion Kodak NTB-3 and stored in a cold dark box (at $4^{\circ}C$) for 3 weeks. After exposure, the samples were developed with Kodak D-19 and stained with uranyl acetate and lead citrate. Routine observation was made with Hitachi HU-11E electron microsocope. The results of the observation were as followings: 1. It was found that the labeled dbcAMP penetrated the egg plasma membrane and dispersed at random in the cytoplasm. 2. It was also observed that most of the labeled dbcAMP was attached to microfibrillar lattices portion of the oocyte cytoplasm. There fore, it is presumed that the receptor of the dbcAMP is localized in the microfibrillar lattices of the oocyte. 3. It also seems that some other cell organells such as mitochondria, Golgi complex, cortical granules are not directly related to the action of the dbcAMP. 4. The labeled dbcAMP was neither observed in the membrane nor in the nucleus. Therefore, it seems that there is no relationship between the concentration of dbcAMP and the nuclear membranous permeability. 5. There was no difference in number of dbcAMP particles when oocytes were cultured for 6 hours and 19 hours. 6. However, it was observed that, in same of the oocytes suppressed in germinal vesicle by dbcAMP for 19 hours, cell organells were moved and concentrated to a small portion of the cytoplasm, and that the morphology of the organells greatly changed to an abnormal. form. Therefore, it is supposed that those oocytes were in the process of degeneration. From the above results, it is expected that dbcAMP penetrated the egg membrane and was bound to the receptor which seems to be located in the microfibrillar lattiees portion, and that this dbcAMP-receptor complex inhibited some enzyme system of the oocytes which are essential for the germinal vesicle breakdown.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.437-441
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• 2012

This study was conducted to find out the optimum container for increasing acclimatization rate of in vitro mass propagated plantlets of Ever-bearing strawberry (Fragaria ${\times}$ ananassa Duch.) via bioreactor. Four types of containers were used such as transparent plastic container (TPC), plug tray (PT), I-pot (IP), and black vinyl pot (BVP). Number of date maintaining soil water content above 10% was five days in TPC, three to four days in BVP, two days in PT, and one day in IP. Survival rate of plantlets was 80% in BVP, 70% in TPC, 55% in IP, and 15% in PT. In TPC, growth increment of plantlets was the greatest among all the tested containers and the lowest in IP. Numbers of runner per plant were 3.3 in BVP, 2.9 in TPC, 1.6 in PT, and 1.2 in IP. Total cost was 44,405,300 won/10 a in BVP, resulting in reducing more 6,659,400 won/10 a than IP's (51,064,700 won/10 a). Around 102,718 plants/10 a were produced by using BVP, suggesting that 30,265.1 plants/10 a more could be produced than IP (72,452.9 plants/10 a). Production cost per plant was 432.3 won in BVP, resulting in reducing 272.5 won than IP's (704.8 won). As a result, BVP was appropriate for acclimatization of in vitro plantlets through bioreactor system.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.3
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• pp.254-260
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• 2015

Changes of the growth, quality and physiological response of Chinese cabbage cv 'Chunkwang' in response to five different temperature treatments based on climate change scenario were investigated during the growing season. The treatments consisted of normal year temperature $-2.0^{\circ}C$ (I), normal year temperature (II; Control group), normal year temperature $+2.0^{\circ}C$ (III), normal year temperature $+4.0^{\circ}C$ (IV), and normal year temperature $+6.0^{\circ}C$ (V). Regarding fresh weight, number of leaves, and leaf area were high in group IV, and V before the head formation stage, but it has decreased during the later growth period. Rate of frangibleness sympton was the highest in group V as 85.7%, and it was decreased in group IV (64.3%), group III (28.6%), group II (14.3%), and group I (7.1%). Regarding photosynthetic rate, group III, IV, and V showed relatively high photosynthetic rate at 20 DAP but it was reduced dramatically during the later growth period. Transpiration and stomatal conductance showed the similar trend with the photosynthetic rate. When comparing the chlorophyll fluorescence reaction of each treatment group at 50 DAP, Fv/Fm in group I was highest as 8.04 among all treatment groups and the lowest in group IV as 7.15.

• Korean Journal of Ecology and Environment
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• v.39 no.2 s.116
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• pp.167-177
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• 2006

Both fish community and water quality in Lake Doam were investigated from September 2004 to August 2005. The turbidity of Lake Doam located in the upper region of the Songchun River in the South River system, Korea was high whole year due to the effects of distributed non point source pollutions in the watersheds. During the experimental periods, mean concentration of chlorophyll-a in epilimnetic layer (0 ${\sim}$ 5 m) was 18.5 ${\mu}g\;L^{-1}$ and transparency ranged from 0.3 m to 2.4 m. Average TP and TN concentrations were 111 ${\mu}g\;L^{-1}$ and 4.1 $mg\;L^{-1}$, respectively. Lake was classified as eutrophic state based on the nutrient concentrations suggested by U.S.EPA (1976). Total number of fish collected in Lake Doam was 9,600 individuals in 26 species of 6 family. Both dominant and subdominant species in the lake were P. herzi (34.9%) and Z. platypus (22.5%), respectively. Occurrence of water column species was high at upper region of the lake, whereas benthic type of species highly ,appeared in downstream area. The different fish assemblage between upper and lower area would be considered as the difference of bottom substrate and concentrations of suspended solids. In addition high appearance of Comat type of fish that is hybrid between gold fish (C, auratus) and C. auratus was found in the lake. It was unclear the reasons that high proportion of mutant species appeared in the lake. More researches are required in this area in future.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.455-461
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• 2002

limiting nutrients for phytoplankton were determined by dissored inorganic nitrogen/phosphorous (DIN/DIP) in situ and algal bioassay experiment in the Seomjin River estuary during a study period from March 1999 to October 2001. DIN/DIP ranged from 14.7 to 681.1 during the study period. DIN/DIP was over 16 at the upper and middle estuarine region where salinity was lower than 25 psu and chlorophyll a concentration was high, probably indicating P-limitation in this region while the ratio was less than 16 at the high saline (> 25 psu) region, reflecting the supply of DIP from Gwangyang Bay and thereby indicating N-limitation at the lower estuarine region. These results suggested that the spatial distribution of DIN/DIP in the study region was controlled by the high supply of phosphate from Gwangyang Bay, the low input from Seomjin River and the active uptake by phytoplankton within the estuarine system. The bioassay experiments using Skeletonema costatum, Thalaasiosira rotula and in situ phytoplankton assemblage displayed relatively higher growth of phytoplankton in the P-added culture media, indicating P-limitation. This result was well consistent with the spatial distribution of inorganic nutrients. S. costatum showed a rapid adaptation to the low salinity compared to other phytoplankton species. This phenomenon seemed to account for the strong (> $90\%$ in total cell number) S. costatum bloom in autumn in this estuary. Moreover, although phytoplankton growth rate was higher in the P-added culture media at the end of culture experiment of in situ phytoplankton, the fast growth in the trace metal-added media at the beginning of the experiment suggested a possibility of limitation by other micro-nutrients such as trace metal and vitamin etc.

• Journal of Home Health Care Nursing
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• v.5
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• pp.84-99
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• 1998

The objectives of this study are to identify the actual educational contents of management for head nurse and to propose the educational subjects according to identity the needs of head, charge, and staff nurses. The subjects were investigated the actual Management Development Programs and educational needs of head nurses and prospective nurse manager(charge nurse, staff nurse with a lot of clinical experiences) in general hospitals. The tools were composed of two questionnaires: One was developed from the literature review for making items to measure actual situation. The other was revised Katz's model for measurement of educational needs. The first respondents of actual situation were 27 general hospitals with over 400 beds in Seoul and the second respondents were 89 head nurses, 67 charge nurses and 136 nurses at 3 hospitals by convenient sampling out of 27 general hospitals. Data were collected by telephone interview, mail questionnaire and visiting from 7th of October through 30th of November in 1997. In data analysis, general characteristics of the respondents and actual status of Management Development Programs were analyzed by frequency and percentage. Educational needs according to general characteristics were analyzed by ANOVA The results were as follows: 1. Actual situation of Management Development Program 1) Seven hospitals(26%) had Management Development Program for prospective managers and 14 hospitals (52%) for head nurses. 2) Education Department existed in 14 hospitals (52%). 3) One hospital(4%) had top level managers took part in the Management Development. 4) Two hospitals selected head nurse, who had finished courses of Management Development. Eight hospitals(30%) assessed educational needs. The assessment tools consisted of making a question via questionnaire(75%), determining at department meeting(12%) and interview(13%). 5) Educational programs had 3 types: 10 lecture type, 7 discussion type and 4 role play type programs. 6) One hospital evaluated the change of learner's attitude. 7) Four hospitals scored educational point, but that was measured only by attending. 8) Actual Management Development Programs were as follows. parenthesis indicates the number of hospitals. (1) Management Development Programs for Prospective manager. Role perception of Middle level Manager (1) . Role reconstruction of Nurse Manager (1). Workshop for Charge Nurse (1). Nursing Delivery System and Nursing Process (1). Communication (1). Motivation (1) (2) Management Development Programs for Head nurse.. Head nurse's Role (5). Administrative Work (7). Service Education (4). Prevention and Countermeasure of Nursing Incidence (3). Appraisal (3) 2. The results of needs on Management Development subject 1) The educational needs of all respondents on 3 skill domains showed positive agreement to strongly positive agreement. 2) High priority(more than 4.5) items were 12 of 24 Human skill items(50%), 1 of 6 Technical skill items(16%), and 2 of 13 Conceptual skill items (15%). 3) Out of high priority items, 8 items were instituted. 4) All respondents showed high needs on 3 skill domains regardless of 3 positions (head nurse, charge nurse, and nurse). Educational needs of Human skill domain, according to position were 108. S, 108.7, 106.8 (mean score = 72) , needs of Technical skill domain were 26.5, 26.6, 26.I(mean score=18), and needs of Conceptual skill domains were 56.9,56.7, 55.1(mean score=39). 5) Needs of 3 skill domains according to clinical career showed significant difference. Out of respondents, nurses with career of over 16years showed lowest degree of needs in Human skill domains(F=4.47, P=.004) and Conceptual skill domain(F=2.93, P=.034). 6) Educational needs according to educational background were not significant difference. But out of respondents, nurses educated at 3-year junior college relatively showed lowest needs in all of the 3 skill domains. With the above-mentioned findings, further study is necessary for generalization of this study at hospitals with different bed size and location. Also it is needed to study about management skill of nurse and charge nurse, and effective educational method.

• Journal of The Korean Association For Science Education
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• v.33 no.7
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• pp.1367-1384
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• 2013

The purpose of this study was to develop a Science Academic Emotion Scale for Elementary Students. To make a scale, authors extract a core of 14 emotions related to science learning situations from Kim & Kim (2013) and literature review. Items on the scale consisted of 14 emotions and science learning situations. The first preliminary scale had 174 items on it. The number of 174 items was reduced and elaborated on by three science educators. Authors verified the scale using exploratory factor analysis, confirmatory factor analysis, inter-item consistency and concurrent validity. The second preliminary scale consisted of 141 items. The preliminary scale was reduced to seven factors and 56 items by applying exploratory factor analysis twice. The seven factors include: enjoyment contentment interest, boredom, shame, discontent, anger, anxiety, and laziness. The 56 items were elaborated on by five science educators. The scale with 56 items was fixed with seven factors and 35 items to get the final scale by applying confirmatory factor analysis twice. Except for Chi-square and GFI (Goodness of Fit Index), other various goodness of fit characteristics of the seven factors and 35 items model showed good estimated figures. The Cronbach of the scale was 0.85. The Cronbach of seven factors are 0.95 in enjoyment contentment interest, 0.81 in boredom, 0.87 in shame, 0.82 in discontent, 0.87 in anger, 0.77 in anxiety, 0.81 in laziness. The correlation coefficient was 0.59 in enjoyment contentment interest, 0.54 in anxiety, 0.42 in shame, and 0.28 in boredom, which were estimated using the Science Academic Emotion Scale and National Assessment System of Science-Related Affective Domain (Kim et al., 1998). Based on the results, authors judged that the Science Academic Emotion Scale for Elementary Students achieved an acceptable validity and reliability.