• 농업생명과학연구
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• 제44권6호
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• pp.39-44
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• 2010

An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, $0.5mgL^{-1}$ 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins ${\alpha}-napthalene$ acetic acid (NAA), indole acetic acid (IAA) and ${\beta}-indolebutyric$ acid (IBA) (0.5 and $1.0mgL^{-1}$). The $1.0mgL^{-1}$ IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.

• Journal of Plant Biotechnology
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• 제39권2호
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• pp.114-120
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• 2012

본 실험은 약리적 효능이 뛰어난 홍경천의 기관 (액아가 포함된 줄기 조직)배양을 통한 대량증식 방법의 일환으로 액아배양을 통한 다신초를 유도, 증식조건에 관해 BA와 GA3의 영향을 조사하였고 신초증식에 필요한 적정 sucrose의 농도를 확인하였다. 그 후 적절한 기내 발근 및 토양순화 조건을 탐색하여 최적의 홍경천의 생산 조건을 확립하였다. 다신초의 유도 및 증식은 $GA_3$와 BA를 혼합 처리가 효과적이었다. 신초증식에 효과적인 sucrose의 농도는 50 g/L가 첨가된 배지였으며, 평균 7 cm 이상의 신장을 보였다. 기내 발근은 옥신류 식물생장조절물질 무첨가 배지에서 가장 높은 발근길이와 개수를 나타냈다. 기내발근된 유식물체의 토양순화는 모래상을 제외하고는 모두 양호한 생장을 보였고, 특히 모래, 피트모스, 펄라이트가 모두 첨가된 배합토에서 가장 양호한 생장을 보였다. 이상의 결과는 수년전 국내에 도입하려다 실패한 약용식물인 홍경천의 기내배양을 통한 재배의 가능성을 제시하는 것이고 더 나아가 다양한 생물소재의 활용 방안의 기초재료로 활용될 것으로 보여진다.

• 한국환경농학회지
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• 제34권4호
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• pp.288-293
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• 2015

한반도에 태풍내습이 빈번한 7, 8, 9월에 참다래(키위)가 조기낙엽되었을 때 당년 액아의 재발아, 익년발아, 개화 등 수체생장에 미치는 영향을 알아보고자 하였다. 비가림조건에서 포트재배(13L)된 골드키위 신품종 '골드러쉬' 착과유목 1-2년생을 대상으로 하고, 적엽처리는 7, 8, 9월에 각각 0(무처리), 50, 100% 잎을 제거하는 것으로 하였다. 적엽처리 30 일뒤 액아 재발아율은 적엽수준에 비례하여 증가하였으며, 시기적으로는 7월이 가장 높았고 8월과 9월로 갈수록 낮았다. 전년도 조기낙엽에 따른 이듬해 발아율은 처리간에 차이가 없었다. 무처리구의 주당 평균 결과지율과 결과지당 꽃수는 각각 27.4%와 2-3개였다. 그러나 전년도 8월과 9월의 100% 적엽구의 결과지율과 결과지당 꽃수는 무처리에 비해 크게 감소하였다. 특히, 8월 16일 100% 적엽구에서는 전혀 꽃이 형성되지 않아 태풍에 의해 조기낙엽이 이 시기에 발생할 경우 개화량 감소로 인한 생산량 감소는 불가피할 것으로 판단된다. 참다래 생산농가에서는 7월 이후에 낙엽피해가 발생하지 않도록 반드시 파풍망, 비가림 등 바람피해 예방시설을 갖추어야 할 것으로 판단된다.

• Journal of Plant Biotechnology
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• 제44권1호
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• pp.42-48
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• 2017

본 연구는 윤판나물의 효과적인 기내 증식을 위한 배양 최적 조건을 구명하기 위해 수행되었다. 신초 유도 및 생장에 영향하는 배지(MS, B5 및 WPM) 및 절편체 종류에 따른 실험에서 줄기 유도는 액아 절편을 식물생장조절물질 무첨가 MS 배지에 배양 시 절편 당 2.5개로 가장 좋았고, 줄기 신장은 정아 절편을 식물생장조절물질 무첨가 MS 배지 배양 시 7.2 cm로 가장 효과적이었다. BA 침지 처리 시 신초 유도 효과는 액아배양 유래 신초를 BA 침지 무처리구에서 신초 유도(2.29개/절편) 및 신초 신장(7.28 cm)이 가장 효과적이었으며, BA 침지 시간이 길어짐에 따라 신초 유도 및 길이 생장은 감소하였다. 발근에 영향하는 IBA 농도 효과는 정아유래 신초를 1.0 mg/L 처리구에서 배양 시 최대 발근율(100%) 및 뿌리 유도수(21.3개/절편)를 보였으며, 정아 혹은 액아 유래 신초지 종류에 따른 발근율의 차이는 크지 않은 것으로 관찰되었다. 발근된 식물체는 상토로 이식하여 형태적인 변이 없이 정상적인 생장을 보였다.

• Journal of Plant Biotechnology
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• 제41권4호
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• pp.236-241
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• 2014

An efficient regeneration protocol for stem disc culture of Populus euramericana, which is important species for bioenergy resource in agroforestry, was established. The number of explants that were obtained and the number of explants that regenerated varied with the genotypes. However, in all the genotypes, stem disc culture produced more regenerated shoots than did in axillary bud culture. A comparison of the effects of cytokinin type and concentration on shoot regeneration in different explants (i.e., petiole, leaf, and root segments of P. euramericana) revealed that a concentration of $0.002mg\;l^{-1}$ thidiazuron (TDZ) used on petiole segments resulted in the greatest shoot regeneration (95.83%). The hormonal requirements for the greatest shoot regeneration in the three explant types varied. Different concentrations of $AgNO_3$ and $CoCl_2$ were added separately to the medium to stop the yellowing and subsequent necrosis of the regenerated shoots. Lower concentrations (3 and $5mg\;l^{-1}$) of these compounds improved shoot regeneration and elongation, compared with the control. The in vitro-regenerated shoots were transferred to rooting medium and subsequently acclimatized. The highly efficient regeneration system of P. euramericana reported here can be used for mass propagation of this recalcitrant for regeneration, economically important tree species.

• Journal of Plant Biotechnology
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• 제30권3호
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• pp.263-267
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• 2003

This study was conducted to evaluate the effect of thidazuron(TDZ) on shoot proliferation and growth from axillary buds of 20-years-old Corylopsis coreana. Shoots proliferation was effectively achieved on WPM(Woody Plant Medium) supplemented with 0.03∼0.1mg/L TDZ. The highest shoot number(6.5$\pm$0.7) was obtained on 0.1mg/L TDZ treatment. On the TDZ medium shoots formed as clusters less than 1cm in height and therefore needed to subculture on GA$_{3}$ containing medium to induce elongation. In consecutive cultures, phenolic compounds were excreted at the proximal part of the explants and inhibited growth of the explants. Growth inhibition by the compounds was overcome using liquid and paper bridge culture system. About 60％ of the elongated shoots rooted on half- strength MS medium containing IBA. Generally, IBA was mire effective on in vitro rooting than NAA with optimal range of 0.5mg/L to 1.0mg/L. Rooted plantlets were transferred in an artificial soil(vermculite) and acclimatized in high humidity greenhouse condition. Survival rate differed greatly depending on rooting types of the explants. Two types of rooting were observed. The first type was direct rooting from the explants. The second type was callus formation followed by rooting from the callus. The explants showing the 1st type rooting survived can be multiplicated in vitro by TDZ treatment followed by elongation with GA$_{3}$ and rooting with IBA.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.24-24
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• 2021

The Cassava (Manihot esculenta Crantz) is one of the major food crops in the tropical or subtropical regions. Recently, clean planting materials of improved cassava cultivars are in high demand. Problems in the propagation of cassava are virus vulnerable and low rates of seed germination. Thus, the study was undertaken to develop an efficient in vitro mass propagation protocol of Manihot esculenta Crantz. So we tried to optimize protocols for mass production from axillary buds of Cassava. Young and actively growing stem segments were excised from adult plants of cassava. Samples were cut into a 3~4 cm nodal segments with axillary buds, and cultivated in the different medium supplemented with various plant growth regulators for 4 weeks. For shoot multiplication, axillary buds approximately 1 cm in length were taken from in vitro derived shoots and subcultured. After 4~6 weeks, the shoot generation rate showed 55.6%. The shoot number and its length was 1.0/explant and 2.3 cm in the most favorable medium composition. The auxin β-indolebutyric acid(IBA) 0~2.0 mg/L was proved to be effective on root development. Plantlets with fibrous roots easily generated tuberous roots in vitro. The tuberous roots were induced only when both kinetin and IBA were used in combination. after 8 weeks, the root generation rate showed 100%. The root number and its length was 17.2/explant and 2.2 cm in the most promising medium composition. Our experiments confirmed that in vitro growth and multiplication of plantlets could depend on its reaction to the different medium composition, and this micropropagation techniques could be a useful system for healthy and vigorous plant production.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.247-257
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• 2005

An efficient, rapid and large-scale in vitro clonal propagation of agronomically important Indian cereal crop genotypes (NSH27 & K5) of Sorghum bicolor (L.) Moench. by enhanced shoot proliferation in shoot tip segments was designed. MS medium fortified with plant growth regulators and coconut water markedly influenced in vitro propagation of Sorghum bicolor. In vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), 5% coconut water and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of shoot tip segments on a similar medium enabled continuous production of more than 100 healthy shoots with similar frequency. When the healthy shoot clumps were cultured on MS medium fortified with 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), ${\alpha}$-naphthaleneacetic acid ($2.7\;{\mu}M$), ascorbic acid ($30.0\;{\mu}M$) and 5% coconut water, a rapid production of axillary and adventitious buds was developed after 8 wk culture. More than 300 shoots were produced 10 wk after culture. Rooting was highest (100%) on half strength MS medium containing 22.8 mM IAA. Micropropagated plants established in garden soil, farmyard soil and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics. These plants grew normally without showing any traits.

• 한국자원식물학회지
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• 제28권6호
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• pp.690-696
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• 2015

We micropropagated pear (Pyrus species) using shoot tips and nodal explants from three pear genotypes. The ability to establish shoot tip cultures, proliferate shoots, induce rooting, and acclimatize the resulting plantlets are all elements of in vitro micropropagation. Shoots were induced from shoot tips on Murashige and Skoog medium (MS) with five different plant growth regulator combinations. The highest shoot formation rates were achieved for the three genotypes using MS supplemented with 1.0 mg/L N⁶-benzyladenine (BA) and 0.1 mg/L gibberellic acid (GA₃). The maximum shoot number and shoot length for the three cultivars were recorded with 2.0 mg/L BA and 0.2 mg/L indole-3-butyric acid (IBA) in multiplication medium using nodal explants produced from microshoots. Nodal explants with one or two axillary buds cultured for three weeks initiated roots on medium supplemented with various concentrations of 1-naphthaleneacetic acid (NAA) or/and IBA in half-strength MS medium for adventitious rooting. The highest rooting response was with the combination of 0.2 mg/L NAA and 0.2 mg/L IBA. A combination of NAA and IBA resulted in a significant increase in the rooting ratio over NAA or IBA alone. In this medium, the root formation rate according to ranged from 68.9% for the BaeYun No. 3 genotype to 51.8% for the Hwanggeum genotype. We also investigated the influence of the concentration the polyamine phloroglucinol in rooting medium. For all three genotypes, the highest rooting ratio, longest root length, and greatest root number were observed in the treatments with 75-150 mg/L phloroglucinol. Most rooted plants were acclimatized successfully.

• 식물조직배양학회지
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• 제25권1호
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• pp.33-36
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• 1998

온실에서 생육하고 있는 Guzmaniz cv. Cherry의 신초를 채취하여 기내에서 대량번식하였다. 온실에서 생장한 신초는 초대배양에서 약 80% 정도로 오염율이 매우 높았다. Cytokinin이 단용으로 첨가된 배지에서 재생된 신초의 증식은 BA 2.0mg/L 첨가배지가 신초수 6.6개로 가장 좋았으며, kinetin 0.5mg/L 첨가배지와 BA 0.5~1.0 mg/L 첨가배지도 증식이 양호하였다. 신초증식에 양호한 cytokinin 과 농도를 선택하여 IAA와 혼용첨가한 배지에서는 kinetin보다 BA를 첨가한 배지가 전반적으로 신초증식이 높았으며, BA 2.0mg/L 단용첨가보다 BA 2.0mg/L 와 IAA 0.1~0.5mg/L를 혼용첨가하였을 때, 신초의 증식이 증가하였다. BA 2.0mg/L와 IAA 0.1mg/L 를 첨가한 배지에서 8.7개의 가장 높은 신초증식율을 나타냈었고, BA 2.0mg/L 와 IAA 0.5~1.0mg/L를 첨가한 배지도 신초증식이 매우 높았다. BA 0.5 mg/L와 IAA 0.1mg/L를 첨가한 배지에서, 80rpm으로 액체회전배양하는 것이 신초수가 12.4개로 액체정체배양이나 고체배양보다 증식율이 월등히 높았다. 신초의 발근은 IBA 2.0 mg/L첨가한 배지가 발근율 100%, 뿌리수 4.14개, 뿌리길이 1.53 cm로 발근이 가장 좋았다.