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http://dx.doi.org/10.5010/JPB.2017.44.1.042

Effect of kinds of medium and plant growth regulators for plantlets regeneration by bud culture in Disporum sessile  

Lee, Na-Nyum (Forest Biotechnology Division, National Institute of Forest Science)
Kim, Ji-Ah (Forest Biotechnology Division, National Institute of Forest Science)
Kim, Tae Dong (Forest Biotechnology Division, National Institute of Forest Science)
Kim, Yong Wook (Forest Biotechnology Division, National Institute of Forest Science)
Publication Information
Journal of Plant Biotechnology / v.44, no.1, 2017 , pp. 42-48 More about this Journal
Abstract
This study was conducted to establish the optimal condition for in vitro propagation of Disporum sessile. In the experiments with different kinds of media (MS, B5 and WPM) and explant types for shoot induction and elongation, the highest number of shoot inductions (2.5/explant) was shown when the axillary bud explants were cultured on MS medium without plant growth regulators (PGRs). The best shoot elongation (7.2 cm) was also obtained when the apical buds were cultured on MS medium. The effect of BA pulse treatments with in vitro shoots was also examined. The highest in shoot induction (2.29/explant) and elongation (7.28 cm) was observed when the shoots from axillary buds were cultured on the media without PGRs. However, it decreased with increasing duration of BA pulse-treatment. The highest rooting rate (100%) and number of root inductions (21.3/explant) were achieved with 1.0 mg/L indole-3-butyric acid (IBA) treatment, whereas no differences were observed by different shoot types. The regenerated in vitro plantlets were hardened and successfully established in soil.
Keywords
Axillary bud; BA pulse treatment; Micropropagation; Plant regeneration; Shoot elongation;
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Times Cited By KSCI : 6  (Citation Analysis)
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