Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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An Efficient Plant Regeneration System for Sorghum bicolor - a Valuable Major Cereal Crop

  • Baskaran P. (Plant Biotechnology unit, Department of Plant Science, School of Life Sciences, Bharathidasan University) ;
  • Jayabalan N. (Plant Biotechnology unit, Department of Plant Science, School of Life Sciences, Bharathidasan University)
  • Published : 2005.12.01
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Abstract

An efficient, rapid and large-scale in vitro clonal propagation of agronomically important Indian cereal crop genotypes (NSH27 & K5) of Sorghum bicolor (L.) Moench. by enhanced shoot proliferation in shoot tip segments was designed. MS medium fortified with plant growth regulators and coconut water markedly influenced in vitro propagation of Sorghum bicolor. In vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), 5% coconut water and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of shoot tip segments on a similar medium enabled continuous production of more than 100 healthy shoots with similar frequency. When the healthy shoot clumps were cultured on MS medium fortified with 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), ${\alpha}$-naphthaleneacetic acid ($2.7\;{\mu}M$), ascorbic acid ($30.0\;{\mu}M$) and 5% coconut water, a rapid production of axillary and adventitious buds was developed after 8 wk culture. More than 300 shoots were produced 10 wk after culture. Rooting was highest (100%) on half strength MS medium containing 22.8 mM IAA. Micropropagated plants established in garden soil, farmyard soil and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics. These plants grew normally without showing any traits.

Keywords

References

  1. Ahmad A, Zhong H, Wang W, Sticklen MB (2002) Shoot apical meristem: in vitro regeneration and morphogenesis in wheat (Triticum aestivum L.). In Vitro Cell. Dev Biol-Plant 38: 163-167 https://doi.org/10.1079/IVP2001267
  2. Alexandrova KS, Denchev PD, Conger BV (1996) Micropropagation of switchgrass by node culture. Crop Sci 36: 1709-1711 https://doi.org/10.2135/cropsci1996.0011183X003600060049x
  3. Archana M, Paramjit K (2003) Genotype dependent somatic embryogenesis and regeneration from leaf base cultures of Sorghum bicolor. J Plant Biochem. Biotech 12: 53-56
  4. Bajaj YPS, Reghunath BR, Gopalakrishnan PK (1993) Elettaria cardamomum Maton (cardamom): Aromatic compounds, in vitro culture studies and clonal propagation. In: Bajaj YPS (ed), Biotechnology in Agriculture and Forestry: Medicinal and Aromatic Plants IV. Springer-Verlag, Berlin, pp 132-147
  5. Baskaran P, Jayabalan N (2005a) A simple approach to improve plant regeneration from callus culture of Sorghum bicolor for crop improvement. J Agri Biotech 1: 179-192
  6. Baskaran P, Jayabalan N (2005b) An efficient micropropagation system for Eclipta alba - A valuable medicinal herb. In Vitro Cell Dev Biol-Plant 41: 532-539 https://doi.org/10.1079/IVP2005667
  7. Brain KW, Richard AC (1993) Clonal propagation of pink ginger in vitro. Hort Sci 28: 1203
  8. Choi YE, Yang DC, Yoon ES, Choi KT (1998) Plant regeneration via adventitious bud formation from cotyledon explants of Panax ginseng C. A. Meyer. Plant Cell Rep 17: 731-736 https://doi.org/10.1007/s002990050474
  9. Constantine D (1978) Round Table Conference. Gembloux, Belgium, pp134
  10. Deshpande SR, Josekutty PC, Prathapasenan G (1998) Plant regeneration from axillary buds of a mature tree of Ficus religiosa. Plant Cell Rep 17: 571-573 https://doi.org/10.1007/s002990050444
  11. Dunstan DI, Short KC, Thomas E (1978) The anatomy of secondary morphogenesis in culuted scutellum tissues of Sorghum bicolor. Protoplasma 97: 251-260 https://doi.org/10.1007/BF01276697
  12. Elkonin LA, Lopushanskaya RF, Pakhomova NV (1996) Embryogenic callus of Sorghum (Sorghum bicolor (L.) Moench) by amino acids. Maydica 40: 153-157
  13. Gamborg OL, Shyluk JP, Brar DS, constable F (1977) Morphogenesis and plant regeneration from callus of immature embryos of sorghum. Plant Sci Lett 10: 67-74 https://doi.org/10.1016/0304-4211(77)90051-7
  14. Gomez KA, Gomez AA (1976) Statistical procedures for agricultural research with emphasis on rice. International Rice Research Institute. Los Banos, Philippines
  15. Haberlandt G (1902) Culturversuche mit isolierten Pfanzenzellen. Sitzungsber. Math-Naturwiss. KI.Kais. Akad Wiss Wien 111: 69-92
  16. Hagio T (1994) Varietal difference of plant regeneration from callus of sorghum mature seed. Breed Sci 44: 121-126
  17. Heng Z, Wenling W, Mariam S (1998) In vitro morphogenesis of Sorghum bicolor (L.) Moench. Efficient plant regeneration from shoot apices. J Plant Physiol 153: 719-726 https://doi.org/10.1016/S0176-1617(98)80226-5
  18. Inoue M, Maeda E (1980) Effects of auxins and cytokinins on the occurrence of green regions in rice callus cultures. Jpn. J Crop Sci 49: 167-174 https://doi.org/10.1626/jcs.49.167
  19. Kumar UK (1999). Methods in plant tissue culture. Agro Botanica, Bikanes, India, pp 66
  20. Latha R, Ajith A, Srinivasa RC, Eganathan P, Balakrishna P (1998) In Vitro Propagation of Salt-Tolerant Wild Rice Relative, Porteresia coarctata Tateoka. J Plant Growth Regul 17:231-235 https://doi.org/10.1007/PL00007039
  21. Ma H, GU M, Liang GH (1987) Plant regeneration from cultured immature embryos of Sorghum bicolor (L.) Moench. Theor App Genet 73: 389-394
  22. Madhumita J, Mukhopadhyay, Sandip Mukhopadhyay, Sumitra Sen (2002) In vitro propagation of Iphigenia indica, an alternative source of colchicines. Plant Cell TissOrg Cult 69: 101-104 https://doi.org/10.1023/A:1015056803480
  23. Medina R, Faloci M, Marassi MA, Mroginski LA (2004) Genetic stability in rice micropropagation. Biacell 28: 13-20
  24. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  25. Mythili PK, Madhavi A, Reddy VD, Seetharama N (2001) Efficient regeneration of pearl millet (Pennisetum glaucum (L.).R. Br.) from shoot tip cultures. Indian J Exp Biol 39: 1274-1279
  26. Nabors MW, Kroskey CS, McHugh DM (1982) Green spots are predictors of high callus growth rates and shoot formation in normal and in salt stressed tissue cultures of oat (Avenasativa L.). Z Pftanzenphysiol 105: 341-349 https://doi.org/10.1016/S0044-328X(82)80030-5
  27. Ogura H, Shimada T (1978) Preliminary report on shoot redifferentiation from wheat callus. Wheat Inf Serv 45: 26-28
  28. Oldach KH, Morgenstern A, Rother S, Girgi M, Kennedy OM, Lorz H (2001) Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaueum (L.) R.Br.] and Sorghum bicolor (L.) Moench. Plant Cell Rep 20: 416-421 https://doi.org/10.1007/s002990100335
  29. Patil VM, Kuruvinashetli MS (1998) Plant regeneration from leaf sheath cultures of some rabi sorghum cultivars. South African J Bot 64: 217-219 https://doi.org/10.1016/S0254-6299(15)30871-1
  30. Raghavendra Rao RV, Pavan Kumar G, Subba Rao MV, Manga V (2000) Differential in vitro response of genotypes of Sorghum. Phytomorph 50: 253-260
  31. Reghunath BR, Bajaj YPS (1992) Micropropagation of cardamom (Elettaria eardamomum Maton). In: Bajaj YPS (eds), Biotechnology in Agriculture and Forestry, High Tech and Micropropagation III. Springer-Verlag, Berlin, pp 175-198
  32. Sairam RV, Parani M, Franklin G, Lifeng Z, Smith B, MacDougall J, Wilber C, Sheikhi H, Kashikar N, Meeker K, Al-Abed D, Berry K, Vierling R, Goldman SL (2003) Shoot meristem: an ideal explant for Zea mays L. transformation. Genome 46: 323-329 https://doi.org/10.1139/g02-120
  33. Sajina A, Mini PM, John CZ, Babu KN, Ravindran PN, Peter KV (1997) Micropropagation of large cardamom (Amomum subulatum Roxb.). J Spices Aromatic Crops 6: 145-148
  34. Saradamani N, Muralimohan S, Sudhakar R, Pola, Dora, SVVSN (2003) In vitro morphogenesis in cultivated varieties of Sorghum bicolor (L.) Moench. Plant Cell Biol Mol Biol 4: 43-48
  35. Sarma KS, Rogers SMD (1998) Plant regeneration and multiplication of the emergent wetland monocot Juncus accuminatus. Plant Cell Rep 17: 656-660 https://doi.org/10.1007/s002990050460
  36. Seetharama N, Sairam RV, Rani TS (2000) Regeneration of sorghum from shoot tip cultures and field performance of the progeny. Plant Cell Tiss Organ Cult 61: 169-173 https://doi.org/10.1023/A:1006403601498
  37. Sharma VK, Hansch R, Mendel RR, Schulze J (2004) A highly efficient plant regeneration
  38. from commercial cultivars of barley (Hordeum vulgare L.) using meristematic shoot segments excised from germinated mature embryos. Plant Cell Rep 23: 9-16
  39. Shimada T, Yamada Y (1979) Wheat plants regenerated from embryo cell cultures. Jpn J Genet 54: 379-385 https://doi.org/10.1266/jjg.54.379
  40. Skirvin RM, Chu MC, Rukan H (1980) An improved medium for the in vitro rooting of Harbrite Peach. Proc III State Hortic Soc 113: 30-38
  41. Syamala D, Devi P (2003) Efficient regeneration of sorghum, Sorghum bicolor (L.) Moench, from shoot-tip explant. Indian J Exp Biol 41: 1482-1486
  42. Thomas E, King PJ, Portykus I (1977) Shoot and embryo-like structure formation from cultured tissues of Sorghum bicolor. Naturwissenschaften 64: 587 https://doi.org/10.1007/BF00450647
  43. Vasil V, Vasil IK (1981) Somatic embryogenesis and plant regeneration from tissue cultures of Pennisetum americanum and P. americanum $\times$ P. purpureum hybrid. Am. J Bot 68: 864-872 https://doi.org/10.2307/2443193
  44. Vila S.K, Gonzalez AM, Rey HY, Mroginski LA (2003) In vitro Plant Regeneration of Melia azedarach L. Shoot Organogenesis from Leaf Explants. Biologia Plantarum 47: 13-19
  45. Wernicke W, Brettell R (1980) Somatic embryogenesis from Sorghum bicolor leaves. Nature 287: 138-139 https://doi.org/10.1038/287138a0
  46. Wondyifraw T, Surawit W (2004) Micropropagation of Krawan (Amomum krervanh Pierre ex Gagnep). Science Asia 30: 9-150 https://doi.org/10.2306/scienceasia1513-1874.2004.30.009
  47. Zhong H, Wang W, Sticklen M (1998) In vitro regeneration of Sorghum bicolor (L.)
  48. Moench: efficient plant regeneration from shoot apices. J Plant Physiol 153: 719-726